[당선작] 서울대 대학원 교육연구동

문진호(Moon, Jin-ho),백문기(Baek, Moon-gi),한상욱(Han, Sang-uk),장윤규(Jang, Yoon-gyoo) 현대건축사 2003 월간 CONCEPT Vol.- No.52

Ethanol Extract of Ganoderma lucidum Augments Cellular Anti-oxidant Defense through Activation of Nrf2/HO-1

Lee, Yoo-hwan,Kim, Jung-hee,Song, Choon-ho,Jang, Kyung-jeon,kim, Cheol-hong,Kang, Ji-Sook,Choi, Yung-hyun,Yoon, Hyun-Min KOREAN PHARMACOPUNCTURE INSTITUTE 2016 Journal of pharmacopuncture Vol.19 No.1

Objectives: The mushroom Ganoderma lucidum has been widely used as a traditional herbal medicine for many years. Although several studies have focused on the anti-oxidative activity of this mushroom, the molecular mechanisms underlying its activity have not yet been clearly established. The present study investigated the cytoprotective effect of ethanol extract of Ganoderma lucidum (EGL) against oxidative stress (hydrogen peroxide, $H_2O_2$) and elucidated the underlying mechanisms in a C2C12 myoblast cell line. Methods: Oxidative stress markers were determined by using the comet assay to measure reactive oxygen species (ROS) generation and deoxyribonucleic acid (DNA) damage. Cell viability and Western blotting analyses were employed to evaluate the cellular response to EGL and $H_2O_2$ in C2C12 cells. Transfection with nuclear factor erythroid 2-related factor 2 (Nrf2)-specific small interfering ribonucleic acid (siRNA) was conducted to understand the relationship between Nrf2 expression and $H_2O_2$-induced growth inhibition. Results: The results showed that EGL effectively inhibited $H_2O_2$-induced growth and the generation of ROS. EGL markedly suppressed $H_2O_2$-induced comet-like DNA formation and phosphorylation of histone H2AX at serine 139 ($p-{\gamma}H2AX$), a widely used marker of DNA damage, suggesting that EGL prevented $H_2O_2$-induced DNA damage. Furthermore, the EGL treatment effectively induced the expression of Nrf2, as well as heme oxygenase-1 (HO-1), with parallel phosphorylation and nuclear translocation of Nrf2 in the C2C12 myoblasts. However, zinc protoporphyrin IX, a HO-1 inhibitor, significantly abolished the protective effects of EGL against $H_2O_2$-induced accumulation of ROS and reduced cell growth. Notably, transient transfection with Nrf2-specific siRNA attenuated the cytoprotective effects and HO-1 induction by EGL, indicating that EGL induced the expression of HO-1 in an Nrf2-dependent manner. Conclusion: Collectively, these results demonstrate that EGL augments the cellular anti-oxidant defense capacity through activation of Nrf2/HO-1, thereby protecting C2C12 myoblasts from $H_2O_2$-induced oxidative cytotoxicity.

토끼의 혀혈성 전처치 모델(Ischemic Proconditioning Model)에서 Heat Shock Protein 70의 발현양상과 초산화물 불균등화 효소(Superoxide Dismutase)와의 관계

윤종현,윤호중,전희경,유기동,백상홍,정욱성,채장성,김재형,최규보,홍순조 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9

뇌손상 후 운동신경기능 회복 기전 : 기능적 뇌 자기공명영상의 유용성 The Usefulness of fMRI Study

장성호,한봉수,장용민,변우목,김성호,조윤우 한국뇌학회 2001 한국뇌학회지 Vol.1 No.1

목적: 본 연구는 뇌손상환자에서 기능적 뇌 자기공명영상(functional magnetic resonance imaging, fMRI)을 이용한 대뇌운동피질 뇌지도화를 시행하여, 운동신경기능 회복 기전에 대한 기능적 뇌 자기공명영상의 유용성에 대하여 연구하였다. 방법: 신경학적 증상과 병력이 없는 성인 8명과 뇌손상환자 5인을 대상으로 하였다. 뇌손상환자 중 2명은 뇌종양환자이었으며 1명은 뇌출혈, 1명은 뇌경색, 1명은 뇌낭미충증환자이었다. 기능적 자기공명영상은 1.5T MR scanner로 Blood Oxygen Level-Dependent(BOLD) 기법을 적용하였다. 운동과제는 손가락을 1-2Hz의 주기로 쥐었다 펴기를 반복하도록 하였다. 결과: fMRI를 촬영한 결과 정상인 8인, 즉 총 16개의 손중 15개의 손과 환자 5인의 이환되지 않은 손운동시 반대측 일차 운동피질 꼭지(knob)를 중심으로 일차 감각운동피질(SM1)이 활성화되었다. 정상인 중 1인의 한 손에서 반대측 SM1과 동측 일차 운동피질(primary motor cortex, M1) 및 보조운동영역이 동시에 활성화되었다. 뇌손상환자의 이환측 손운동시 환자 1에서는 일차감각피질(primary sensory cortex, S1), 환자 2와 환자 3에서는 양측 SM1, 환자 4와 환자 5에서는 반대측 SM1이 활성화되었다. 환자 1에서는 손상된 M1의 운동신경기능이 S1으로 이전된 것으로 추정되며, 환자 4와 5에서는 손의 운동신경기능이 피질척수로에 의하여 유지되고 있는 것으로 추정되었다. 그러나 환자 2와 환자 3에서 발견된 동측 운동피질 활성화는 본래 정상적으로 존재하던 운동신경 경로인지 뇌손상 후에 발생한 운동신경기능 회복 경로인지 구분할 수는 없었다. 결론: fMRI을 통한 대뇌 운동피질 지도화는 뇌손상 후 병변 주위로의 재조직되는 회복 기전과 피질척수로에 의해 운동신경기능이 유지되고 있음을 입증하는데에 유용할 것으로 생각된다. Objective: This study investigated the usefulness of functional magnetic resonance imaging (fMRI) in the research of motor recovery mechanisms in brain injured patients. Method: Eight control subjects and five brain injured patients were investigated. Out of five patients, two were with brain tumor, one cerebral hemorrhage, one cerebral infarction, and one neurocysticercosis. fMRI was performed using the blood oxygen level-dependent (BOLD) technique at 1.5 TMR scanner with a standard head coil. The motor activation task consisted of finger flexion-extension exercises in 1-2 Hz cycles. Results: fMRI showed activation of the contralateral primary sensori-motor cortex(SM1) by the hand movements of all control subjects and by the unaffected hand movements of all brain-injured patients. Activation of the supplementary motor area(SMA) by one hand movement was also seen in one control subject. Hand movements of the affected hand of the brain injured patients resulted in activation of the contralateral primary sensory cortex(S1) in patient 1, both SM1 in patients 2 and 3, and the contralateral SM1 in patients 4 and 5. Conclusions: It is concluded that fMRI is useful for the research of perilesional reorganization and sparing of the corticospinal tract during the motor recovery mechanisms after brain injury.

삼화화강암의 미량원소 특성과 연대측정에 관한 연구

장윤호 三陟大學校 1996 論文集 Vol.29 No.1

In this researching area, Iron ore deposit of skarn type is distributed in the line with the part of contact with pungchon limestone of great limestone group in relative rock of the cretaceous Samwha granite. The Granite has a high SiO₂, K₂O, ,Na₂O, contents on the contrary A1₂O CaO MgO TiO₂ low and they are of a uniform composition generally. when we investigate the interrelation of trace elements versus D.I, high differentiation have come out. And we found that Zr, th, Rb have a tendency to increase but Ba, Sr is decrease and Nb, Y, Rb has not systematic change and Rb/Sr versus D.I has a positive relation according as differentiation index increase. At the end, the age dating of this granite is not certain through whole rock isochron because of insufficiencey and alteration sample but I infered it was made in Mesozoic cretaceous.

제주도 고산에서 수용성 에어로졸의 화학적 성분 분석

장광미,이호근,강창희,허철구,박경윤,서명석 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

Atmospheric aerosols were collected by a High Volume Tape Sampler from March 1992 to December 1993 at Kosan, Cheju, korea. The water soluble ion concentrations in aerosol were analyzed. The concentrations of cations(Na+, K+, Ca²+, Mg²+, NH₄+) were determined by an Inductively Coupled Plazma(ICP) or an Atomic Absorption Spectrometer(AAS), and those of anions(Cl-, NO₃-, SO₄²-) were analayzed by the capillary electrophoresis method. The Ca²+, SO₄²-and NO₃- concentrations in spring were higher than those in other seasons. The lowest concentrations of these elements were found in summer, largely due to scavenging by frequent rains. Especially the ca²+ concentration on April was three to four times higher than the annual mean concentration. The enrichment factor(E.F.) of each element was calculated. The annual mean E.F. values of the Ca²+, Mg²+ and Cl- in 1992 were the same as those in 1993 except K+ and SO₄²-. The correlation formula between all cations and anions for the whole period was Anions=0.759xCations+0.066.

청정지역과 도시지역의 오존농도 특성 연구

장광미,강창희,서명석,허철구,박경윤,이호근,김영준 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

A study has been performed on the characteristics of rural and urban surface ozone concentration for the period of March 1992 to February 1993. The monitoring station of rural ozone is located at Kosan, Cheju and other urban monitoring stations are located at Seoul, Pusan and Kwangju. Rural's and urban's ozone data exhibit a distinct features in many ways. First, annual mean of rural ozone concentration is very high(42 ppbv) but urban's are very low(10~15 ppbv). Second, rural ozone data shows a seasonal variation with it's maximum in spring, and minimum in summer, but urban's show a seasonal variation with it's maximum in spring, and minimum in winter. Third, diurnal variation of rural data is very small but that of urban's are very large. Fourth, monthly mean of daily minimum of rural data is not low and shows a large seasonal variation, but those of urban's data are extremely low(<3 ppbv) and have no seasonal variations.

상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol : 식용 식물자원으로부터 활성물질의 탐색 Development of Biologically Active Compound from Edible Plant Sources

장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

현장측정에 의한 공기조화기 에어필터의 성능 평가

정용호,박병윤,손장열 대한건축학회 2004 대한건축학회논문집 Vol.20 No.7

Air filters of air conditioning system play a main role in improving indoor air quality. However there is not enough information about air filters and any official maintenance standards or guidelines for air filters in Korea. The aims of this study are proposal for air filter test methods after installation and basic data about air filter maintenance guidelines or standards. In this study, arrestances, pressure losses and microorganisms between the upstream and the downstream of air filters were measured with digital dust indicators, a micro manometer and blood agar plates. Measurements were conducted before and after washing out pre filters and outdoor air untake ratio varied between 10%, 20% and 30% under actual operating conditions. The results of this study is as follows. Arrestance increased depending on whether the input of outdoor air was added. However some cases did not follow the trend. Pressure loss decreased depending on whether the input of outdoor air was added to AHUs which have built-out type return fans and vice versa in the built-in type. In contrast, the colony counts of microorganisms increased in the downstream of air filters. The rates of increase dropped depending on the addition of the input of OA. Arrestance increased, pressure loss decreased and the rates of increase of microorganisms dropped after washing out prefilters. Medium filter replacement is the main factor for arrestance, prefilter replacement or washing is the main factor for microorganisms but the range of fluctuation in differential pressure was hard to recognize as 1∼4mmAq. As for the results, proper maintenance for air filters, periodical washing out prefilters are important and development air filter performance test methods, standards or guidelines of dust concentration and microorganisms in AHUs after installation are required.

은행잎 추출물이 협심증 환자의 내피 세포 기능에 미치는 장단기 효과

배장호,김기식,이인규,김여희,김민수,김기영,남창욱,김윤년,김권배,정시전 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9