발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

算數·自然 統合敎科 分析 및 指導硏究

姜錫虞,成濟鉉,徐成輔,朴成澤 부산교육대학교 과학교육연구소 1982 科學敎育硏究 Vol.7 No.-

This study is an analysis and a plan for the improvement on a teaching method, a development of the teaching instruments in integrated subject of mathematics and natural science called "Inquiring Life". Thus this study is summarized as follows: (1) An analysis of the integrated textbook. ① The proportion of the time required for teaching mathematics and natural science is 2:1 in our curriculum. However, in the practical guide book of the Inquiring Life, it has been investigated that the time allotted for mathematics is 12 periods more than the time allotted for natural science, furthermore its significant difference is to appear in x^2=3.86 (p<0.05). ② It has been found that there is a meaning of the integration in the first-term text-book, because the quantity of the contents which is integrated in it is 48.7% of the whole. But there is a problem which must be corrected in the second-term textbook, because such quantity is merely 25.6% of the whole. ③ The time required for teaching a field "figures" was 12 periods (9.2%) of the whole in the textbook of the third revised curriculum (1973). However, since the time required for it in the new textbook is only 2.5 periods (2.0%), this is to show a significant difference in x^2=4.62 (p<0.05). ④ It is because of a emphasis on the problem-solving that the time required for teaching the field "relations" has increased more than that of the preceding textbook. ⑤ There is some resistance in "a reclassification of the shaped boards" and "a learning for preservation of a quantity" to construct a possibility of the learning achievment. ⑥ It is found that the contents and the expressions of the integrated textbook is suitable for our pupils, because, in a questionary investigation for their appropriateness, a significant difference is to appear in x^2=9.49 (p<0.05). (2) The teaching method of the integrated textbook. The integrated teaching method is verified to be effective for a group of the dull pupils by showing a significant difference with CR_M=1.99 (p<0.05). (3) A development of the teaching instruments in the integrated textbook. The integrated teaching instruments that are considered to be a interest of pupils, a acceleration of thoughts, a economic condition, a exactness, a convenience, a firmness and anewness, are efficient for our pupils. (4) An estimation for the integrated textbook. An integration of the curriculum and the revision of the form the pupils' cumulative records are demanded in order to get the good estimations for the integrated concepts.

모세관 전기영동법을 이용한 1,2,4-트리메틸벤젠 대사체의 분석

강종성,홍정희,임정미,이용문,장재연 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

방향족 탄화수소인 tnmethylbenzene (TMB)은 그 사용량이 늘어갈 뿐 아니라 직업적으로 폭로되는 양도 증가하고 있으므로 생물학적 모니터링 및 흡수, 대사, 배설에 관한 연구가 중요시되고 있다. 일반적으로 TMB는 간의 산화효소에 의해 하나의 메틸기가 산화되고 이것은 glycine과 포합되어 배설되는 것으로알려져 있다. 본 연구에서는 1,2,4-TMB의 대사체를 합성하고, 모세관의 전기영동법으로 분석할 수 있는 방법을 개발하였다. 모세관 전기영동법으로 흰쥐의 뇨 중에서 1,2,4-TMB의 대사체인 3,4, 2,4, 2,5-dimethylbenzoic acid 및 3,4 2,4 2,5-dimethylhippuric acid를 분석하기 위하여 내경 75㎛, 총길이 35cm (검출기까지 29cm)인 용융실리카 모세관을 15℃로 유지하면서 양단에 10kV의 전압을 걸어주고, 전해질로는 15mM β-CD, 3% 2-프로판올을 포함하는 01m 인산완충액 (pH 7)을 사용하였으며, 검출신호는 UV 210nm와 254nm에서 동시에 모니터링하였다. 뇨 시료의 분석 결과 배설된 1,2,4-TMB의 대사체의 상대량은 3,4-이성질체가 56.&%, 2,4-이성질체가 30.5%, 2,5-이성질체가 12.8%였다. 이 방법은 노동자의 뇨 분석에도 적용될 수 있을 것으로 생각된다. The metabolites of 1,2,4-trimethylbenzene (TMB) were synthesized and determined by capillary electrophoresis (CE). The optimum conditions of CE for the separation and determination of 3,4-, 2,4-, 2,5-dimethylbenzoic acid and 3,4-, 2,4-, 2,5-dimethylhippuric acid from the rat wine were as following: the fused silica capillary(75μm i.d. X 36 cm length, 29 cm to detector) was used and kept at 15℃ The applied voltage was 10kV and compounds were detected at UV 210 nm and 254 nm. The running electrolyte was 0.1 M phosphate buffer (pH 7) contalI1ing 15 mM of β -CD and 3% of 2-propanol. The relative amount of the metabolite of 1,2.4-TMB in the rat urine was 56.7% of 3,4-isomer, 30.5% of 2,4-lsomer and 12.8% of 2,5-isomer. This method can be applied to the analysis of TMB-metabolites in human wine.

RAPD-PCR을 이용한 누에 계통간 유연관계분석

강은영,김성근,이진성,황재삼,서동상 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

We have analyzed the genetic relationship among the silkworm (Bombyx mori) strains using RAPD-PCR. A total of 45 polymorphic RAPD markers amplified from 9 different primers were used to analyze the genetic relationship among the seven silkworm strains. The genetic similarity coefficients among the seven silkworm strains were estimated using the RAPD markers by UPGMA method. The genetic similarity coefficient between 127 and CS120 was 0.261. This value was lower than any other genetic similarity coefficient among the strains tested. And 127-specific RAPD band patterns were observed in 3 primers from 9 polymorphic primers used. This study makes possible phylogenetic identification among the Bombyx mori strains in the molecular level using RAPD-PCR techniques.

혈관모세포종의 임상 분석

김은성,강삼석,이정길,김태선,정신,김재휴,김수한,이제혁 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.5

입냄새 관련 인지도 조사

강은혜,강성귀,최재홍,성진효 조선대학교 구강생물학연구소 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.1

The purpose of this study was to evaluate obtained information regarding a patient's halitosis and investigate about relationship between halitosis and related factors. Halitosis is general term used to describe any disagreeable odor in expired air. Also Halitosis affects a large proporation of population and may cause a significant social of psychological handicap to those suffering from it. Recently many scientist have concern about halitosis. The clinicians will be tried care to patient of halitosis more exactly, if he or she know patient's information about history, symptom, related factors of halitosis. The subjects were 77 who young peoples with 25 years old and using a questionnaire about self and other perception of halitosis, general and oral status, diet controls and cognition etc. The collected data was to analyzed and tested statistical significant. The results were as follows : 1.In presence of halitosis, the self perception was 26% and other Perception was 44%. 2.The group who was indicated malodorer by others is more sensitive in self-bad breath than other groups (p<0.05) 3.In life style, the other perception group have had more smoking than non-other perception group(p<0.05) . 4.The favorite food with strong smell have investigated kimchi , garlic, onion, coffee, spicy curry. Welsh onion and wine. 5. The kinds of food occurred to odor have investigated garlic, onion, coffee, kinds of kimchi, stew, meat and milk. 6. There was not significant correlation among other perception of halitosis and favorite food with strong smell, kinds of food occurred to odor, medical history, present health condition, food impaction to teeth, drinking habit and menstration.

Ubiquitous Sensor Network을 위한 RFID 개인인증 시스템의 구현

이재용,강대성 동아대학교 정보기술연구소 2006 情報技術硏究所論文誌 Vol.14 No.1

In this paper, communication between M2M(machine to machine) in USN(Ubiquitous Sensor Network) becomes hot issue in IT business recently. We propose RFID(Radio Frequency Identification) biometrics system for personal certification to communicate between M2M. This system stores extracted feature vectors from facial image to each tag. It performs comparison and interpretation when facial image is inputted. If bio-information of the tag accords with the extracted feature vectors from the input facial image, it is possible to access the database. Otherwise, authentication is failed. The proposed system is expected to be available for personal authentication in the USN with combination of biometrics, which is face, fingerprint, iris, etc. and RFID system.

이중면 불연속 구형 도파관의 특성 해석

강부식,이청민,장재성 濟州大學校 工科大學 産業技術硏究所 1993 産業技術硏究報告 Vol.4 No.-

Double-plane steps discontinuity in rectangular waveguide is investigated with TE^(x)_(mn) mode analysis. This method accounts for the influence of evanescent fields and power transmission due to higher order modes. For these discontinuites the electromagnetic fields are derived and the scattering matrix is derived by applying the continuous condition in discontinuity. The scattering coefficients of a X-band to Ku-band waveguide transition as well as of resonant iris with finite thickness (t=2.0mm) are calculated and compared with Patzelt's result. The scattering coefficients of the resonant iris (a= 7.112mm, b= 3.556mm, a_(1) = 4.0mm, b_(1) =1.0mm, t=0.5mm) are calculated and compared with Bornemann's results.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.