In-direct localized surface plasmon resonance (LSPR)-based nanosensors for highly sensitive and rapid detection of cortisol

Jeon, Jinwoo,Uthaman, Saji,Lee, Jiyoung,Hwang, Hyejin,Kim, Gibum,Yoo, Pil J.,Hammock, Bruce D.,Kim, Christine S.,Park, Yeon-Su,Park, In-Kyu Elsevier 2018 Sensors and actuators. B, Chemical Vol.266 No.-

<P><B>Abstract</B></P> <P>Over-secretion of cortisol from the adrenal cortex is closely related to acute and chronic stress; thus, rapid and sensitive detection of cortisol in serum is of critical importance for preventing the progression of stress-related diseases. The binding of a biological molecule to the surface of metallic nanoparticles changes the local refractive index and in turn induces a shift in the localized surface plasmon resonance (LSPR) wavelength. Utilizing this phenomenon, we designed a novel disposable LSPR-based cuvette-type sensor for detecting cortisol in serum. The developed cuvette-type nanosensor consists primarily of an assembly of plastic unit sensors coated with gold nanoparticles on a single layer wherein cortisol-conjugated bovine serum albumin (BSA) is immobilized. In this system, a redshift in LSPR wavelength is induced by the binding of cortisol antibody onto cortisol-conjugated BSA immobilized on a gold nanoparticle surface in the nanosensor. In a competitive assay, the nanosensor could rapidly detect cortisol in both a PBS solution and serum (within 20 min) at concentrations ranging from 1 to 10,000 ng/mL (2.759–3 × 10<SUP>3</SUP> nmol/L), which is comparable to conventional enzyme-linked immunosorbent assay (ELISA) which typically requires longer than 4 h and complex sample preparation. Thus, we demonstrated that the LSPR-based nanosensor system developed in this study can provide a useful toolkit for a rapid, highly sensitive and reliable detection of cortisol hormone in a commercially available manner.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel disposable LSPR-based cuvette-type sensor for detecting cortisol in serum. </LI> <LI> Rapid, convenient, and sensitive method for detecting cortisol hormone in serum. </LI> <LI> Detect cortisol in both PBS solution and serum within 20 min at concentrations ranging from 1 to 10,000 ng/mL. </LI> </UL> </P>

Motion of Charged Micro-particle Immersed in Liquid Crystal Controlled by In-plane Field for Electro Paper Display

In-Su Baik,송은경,이승희,Ju-Hwan Choi,Byoung Sun Jung,Sang Youn Jeon 한국전기전자재료학회 2006 Transactions on Electrical and Electronic Material Vol.7 No.1

We have studied the motion of charged micro-particles that are immersed in a nematic liquid crystal (LC) and controlled by in-plane field. The LC is an anisotropic liquid such that the viscosity of the LC depends on flow direction, phase of the LC, and temperature, which affects the motion of the charged particles under the influence of electric field. This study shows that the motion of charged particles mainly depends on the applied voltage and the LC phase, but does not show any significant influence from the initial alignment of LC, although one may expect directional difference in drag force due to interaction between LC and particle. The viscosity changes due to temperature variations in nematic phase also show no signification influence on particle velocity when compared to the effect from varying in-plane field strength.

Motion of Charged Micro-particle Immersed in Liquid Crystal Controlled by In-plane Field for Electro Paper Display

Baik, In-Su,Choi, Ju-Hwan,Jung, Byoung-Sun,Jeon, Sang-Youn,Song, Eun-Kyoung,Lee, Seung-Hee The Korean Institute of Electrical and Electronic 2006 Transactions on Electrical and Electronic Material Vol.7 No.1

We have studied the motion of charged micro-particles that are immersed in a nematic liquid crystal (LC) and controlled by in-plane field. The LC is an anisotropic liquid such that the viscosity of the LC depends on flow direction, phase of the LC, and temperature, which affects the motion of the charged particles under the influence of electric field. This study shows that the motion of charged particles mainly depends on the applied voltage and the LC phase, but does not show any significant influence from the initial alignment of LC, although one may expect directional difference in drag force due to interaction between LC and particle. The viscosity changes due to temperature variations in nematic phase also show no signification influence on particle velocity when compared to the effect from varying in-plane field strength.

한국인에서 혈소판 당단백 Ⅱb/Ⅲa 유전자 다형성과 관동맥 성형술 후 재 협착과의 관계

이민수,이정우,김보영,임대승,강정아,김정희,김윤철,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Platelet aggregation is the final pathway of acute coronary syndrome such as acute myocardial infarction and unstable angina. Platelet glycoprotein IIb/IIIa is a membrane receptor for fibrinogen and yon Willebrand factor and it plays an important role in platelet aggregation and in the pathogenesis of acute coronary syndrome. It is known that polymorphism of the gene that encoding platelet glycoprotein IIb/IIIa(PI^A1/A2) is strongly related to acute coronary syndrome in Caucasian, but not in Koreans. We investigated relationship between platelet glycoprotein llb/Illa gene polymorphism and restenosis of coronary artery after angioplasty in Koreans. Total 371 patients(M=251. F=120) were enrolled. Angioplasty group comprised 143 patients who underwent coronary angioplasty, and in the angioplasty group, restenosis group comprised with the 65 patients who had restenotic lesion over 50% of luminal diameter in follow-up coronary angiography. Normal group comprised 153 patients who had no significant angiographic lesion and variant angina group comprised 75 patients who were positive in ergonovine test. Genomic DNA was extracted from peripheral arterial blood. To determine the frequency of P1^A1/A2 genotype, polymerase chain reaction(PCR) was done and the product was restricted with Mspl. 3%. agarrose gel electrophoresis showed restriction fragment length polymorphism. Clinical profile and risk factor were also reviewed. Among all 371 patients of study group, genotype of only one patients in restenosis group if is proven to be PI^A1/A2 heterozygote. All patients of normal study group, no restenosis group, and the other patients in restenosis group have an PI^A1 homozygote genotype. In our study, platelet glycoprotein IIb/Illa polymorphism has no relationship with restenosis of the coronary artery after angioplasty in Koreans. But the genotypic frequency of platelet glycoprotein IIb/IIIa gene polymorphism in Koreans is concordant with that of previous studies.

태극권 수련이 여성 노인의 보행능력에 미치는 영향

전인수 ( In Su Jeon ),한동욱 ( Dong Wook Han ),박래준 ( Rae Joon Park ),김병로 ( Byung Roh Kim ) 한국스포츠정책과학원(구 한국스포츠개발원) 2006 체육과학연구 Vol.17 No.2

본 연구는 태극권 수련이 여성 노인의 보행능력을 향상시킬 수 있는지 알아보기 위하여 65세 이상 여성 노인 15명(실험군)을 대상으로 태극권 수련 프로그램을 12주 동안 실시하였다. 실험군의 수련 프로그램은 준비운동과 마무리 운동을 포함하여 1회당 50분, 주당 3회를 12주 동안 실시하였다. 태극권 수련이 여성 노인의 보행 능력에 변화가 있었는지를 알아보기 위하여 물리치료 후 태극권을 실시한 실험군과 물리치료만 실시한 대조군의 처치 전과 후의 자료를 근거로 Repeated ANOVA 방법으로 분석하였다. 분석 결과 물리치료 후 태극권을 실시한 실험군의 경우 실험 후, 보행시간 47.5% 감소, 보행속도 54.1% 증가, 분속수 29.2% 증가가 있었고, 대조군의 경우는 보행시간 11.8% 감소, 보행속도 23.9% 증가, 분속수 16.5% 증가가 있었지만 실험군의 변화폭이 컸다. 수련 후 왼쪽다리 보행능력의 경우, 실험군은 한 걸음시간 24.2% 감소, 보폭 21.2% 증가, 보각 15.9% 감소를 보였고, 대조군은 한 걸음시간 6.3% 감소, 보폭 5.1% 증가, 보각 1.7% 증가가 있어 실험군의 변화폭이 컸다. 반면 실험군의 유각기 6.4% 증가, 입각기 3.5% 감소는 대조군의 유각기 3.5% 증가, 입각기 2.0% 감소와 비교해 차이가 없었다. 수련 후의 오른쪽 다리 보행능력의 경우, 실험군은 한 걸음시간 23.4% 감소, 보폭 21.2% 증가를 보였고, 대조군은 한 걸음시간 4.9% 감소, 보폭 5.8% 증가를 보여 실험군의 변화폭이 컸다. 반면 실험군의 유각기 4.8% 증가, 입각기 2.6% 감소, 보각 13.9% 감소의 결과는 대조군의 유각기 5.2% 증가, 입각기 2.8% 감소, 보각 19.0% 감소와 비교해 차이가 없었다. 위의 결과로 볼 때 대조군의 경우도 실험 전에 비해 보행능력이 향상되었지만, 실험군의 변화량이 대조군에 비해 더 큰 것을 볼 수 있다. 따라서 태극권 수련 프로그램이 전반적으로 보행시간 단축, 보행속도 증가, 분속수 증가 등을 포함한 보행능력의 향상에 도움이 되었음을 알 수 있다. The purpose of this study was to investigate the effects of Tai Chi exercise program on the improvement of gait ability of old women. Subjects were fifteen old women living in C-city, above 65 years of age. The subjects were practiced with focusing on the Tai Chi exercise program for 12 weeks. The results of this study were as follows : After exercise program, the experimental group shows ambulation time 47.5% decreased, velocity 54.1% increased and cadence 29.2% increased. The control group shows ambulation time 11.8% decreased, velocity 23.9% increased and cadence 16.5% increased. However, the rate of variance for experimental group is more higher than control group. After exercise program, in case of left leg gait ability, the experimental group shows step time 24.2% decreased, step length 21.2% increased and toe in/out 15.9% decreased. The control group shows step time 6.3% decreased, step length 5.1% increased and toe in/out 1.7% increased. Even the rate of variance for experimental group is more higher than control group, there is no difference between the experimental group for swing 6.4% increased, stance 3.5% decreased and the control group for swing 3.5% increased, stance 2.0% decreased. After exercise program, in case of right leg gait ability, the experimental group shows step time 23.4% decreased, step length 21.2% increased. The control group shows step time 4.9% decreased, step length 5.8% increased. Even the rate of variance for experimental group is more higher than control group, there is no difference between the experimental group for swing 4.8% increased, stance 2.6% decreased, toe in/out 13.9% decreased and control group for swing 5.2% increased, stance 2.8% decreased, toe in/out 19.0% decreased. Even the control group shows the slightly improvement for the gait ability as well, after comparing the rate of variance between two group, we can see that the experimental group has more higher variance than control group. Therefore, the Tai Chi exercise program could be given to the improvement for the changed gait ability of ambulation time, velocity, cadence and etc. in the old woman. Futhermore, it is reasonable program that can continue health care for old woman using Tai Chi exercise program. And then it is necessary to develop much better program than now for the old adult`s health and to provide the Tai Chi exercise.

유기용제 취급근로자들의 요중대사물질과 말초임파구 자매염색분체교환 발현빈도에 관한 조사연구

김돈균,황인경,류철인,이수일,정갑열,이용환,이충렬,현원일,김석봉,전용덕 大韓産業醫學會 1990 대한직업환경의학회지 Vol.2 No.1

저자들은 유기용제 취급여성근로자 90명을 대상으로 1988년 7월부터 1989년 8월까지 말초혈액임파구에서의 자매염색분체교환의 발현빈도를 조사하고 이들의 업종, 근속연수, 요중마뇨산 농도등이 자매염색분체교환의 발현빈도에 미치는 영향을 조사하였으며 그 결과를 요약하면 다음과 같다. 1. 유기용제 취급근로자들의 말초혈액임파구에서의 자매염색분체교환의 발현빈도는 대조군에 비하여 유의하게 증가되었다. 2. 말초혈액임파구에서의 자매염색분체교환의 발현빈도가 가장 높은 업종은 프라스틱제품 제조업이었다. 3. 근속연수가 말초혈액임파구에서의 자매염색분체교환의 발현빈도에 미치는 영향은 현저하지 않았다. 4. 요중마뇨산농도와 말초혈액임파구에서의 자매염색분체교환의 발현빈도간에는 유의한 상관관계가 있었다. In order to know the possibility of utilizing the sister chromatid exchanges as an index which could evaluate the effect of organic solvents on the health in industrial workers, the authors studied the effects of the inductivity of sister chromatid exchanges in peripheral lymphocytes from 90 female workers expoxed to organic solvents and 20 non-exposed female workers. The results obtained were as follows : 1. The frequency of sister chromatid exchanges in peripheral lymphocytes from 90 female workers exposed to organic solvents was significantly increased in comparison with 20 control subject. 2. The frequency of sister chromatid exchanges was significantly increased in the workers who were employed in the manufacture of plastic materials than the other manufactures. 3. There were no significant differences in the frequency of sister chromatid exchanges by carriers of the exposed workers.

Spontaneous Pt Deposition on Defective Surfaces of In₂O₃ Nanocrystals Confined within Cavities of Hollow Silica Nanoshells: Pt Catalyst-Modified ITO Electrode with Enhanced ECL Performance

Cho, Young Shin,Kim, Soo Min,Ju, Youngwon,Kim, Junghoon,Jeon, Ki-Wan,Cho, Seung Hwan,Kim, Joohoon,Lee, In Su American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.24

<P>Although the deposition of metallic domains on a preformed semiconductor nanocrystal provides an effective pathway to access diverse hybrid nanocrystals with synergistic In2O3 metal/semiconductor heterojunction interface, those reactions that take place on the surface of semiconductor nanoscrystals have not been investigated thoroughly, because of the impediments caused by the surface-capping organic surfactants. By exploiting the interfacial reactions occurring between the solution and nanoparticles confined with the cavities of hollow nanoparticles, we propose a novel nanospace-confined strategy for assessing the innate reactivity of surfaces of inorganic semiconductor nanoparticles. This strategy was adopted to investigate the newly discovered process of spontaneous Pt deposition on In2O3 nanocrystals. Through an in-depth examination involving varying key reaction parameters, the Pt deposition process was identified to be templated by the defective In2O3 surface via a unique redox process involving the oxygen vacancies in the In2O3 lattice, whose density can be controlled by high-temperature annealing. The product of the Pt-deposition reaction inside the hollow silica nanoparticle, bearing In2O3-supported Pt catalysts inside the cavity protected by a porous silica shell, was proved to be an effective nanoreactor system which selectively and sustainably catalyzed the reduction reaction of small-sized aromatic nitro-compounds. Moreover, the surfactant-free and electroless Pt deposition protocol, which was devised based on the surface chemistry of the In2O3 nanoparticles, was successfully employed to fabricate Pt-catalyst-modified ITO electrodes.with enhanced electrogenerated chemiluminescece (ECL) performance.</P>

DYNAMIC RESPONSE OF CARBON NANOTUBES DISPERSED IN NEMATIC LIQUID CRYSTAL

YOUNG HEE LEE,SANG YOUN JEON,KYUNG AH PARK,IN-SU BAIK,SEOK JIN JEONG,SEOK HO JEONG,안계혁,SEUNG HEE LEE 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2007 NANO Vol.2 No.1

The alignment and dynamic response of carbon nanotubes (CNTs) in nematic liquid crystal (NLC) medium induced by strong electric field have been observed through polarizing optical microscope. Density-functional calculations suggest that LC molecule anchors helically to the CNT wall to enhance π-stacking with a binding energy of nearly -2.0 eV due to a considerable amount of charge transfer from LC molecule to CNT, resulting in the formation of excess charges and permanent dipole moment in CNTs. Under strong electric field, the motion of CNTs distorted the director of adjacent LC molecules. Our detailed analysis of dynamics revealed that the four-lobe textures in vertical cell and two vertical stripes in in-plane switching cell were strongly correlated, i.e., the side view of textures by the vertical motion of CNTs in vertical cell was similar to the textures in in-plane switching cell. Interestingly, the magnitude of textures in microscope was strongly dependent on the size of CNTs and theapplied field strength. The statistical size distribution of textures similar to that of CNTs provided information for the degree of dispersion of CNTs.

Low serum bilirubin level predicts the develop-ment of chronic kidney disease in patients with type 2 diabetes mellitus

( Kang Hee Ahn ),( Sang Soo Kim ),( Won Jin Kim ),( Jong Ho Kim ),( Yun Jeong Nam ),( Su Bin Park ),( Yun Kyung Jeon ),( Bo Hyun Kim ),( In Joo Kim ),( Yong Ki Kim ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.5

Background/Aims: We evaluated whether serum bilirubin levels can predict the development of chronic kidney disease (CKD) in patients with type 2 diabetes mellitus (T₂DM). Methods: This was a retrospective observational longitudinal study of patients presenting at the Pusan National University Hospital. A total of₃₄₉ patients with T₂DM and preserved kidney function (estimated glomerular filtration rate ≥ 60 mL/min/1.73 m²) were enrolled. The main outcome was the development of CKD stage 3 or greater. The patients were divided into four groups according to the quartiles of the total serum bilirubin levels at baseline. Results: The group with the lowest range of total serum bilirubin level (Q₁) showed the highest cumulative incidence of CKD stage 3 or greater than that of the other lower quartiles (Q₁ vs. Q₄; hazard ratio [HR], 6.75; 95% confidence in-terval [CI], 1.54 to 29.47; p = 0.011). In multivariate analysis, the risk of developing CKD stage 3 or greater was higher in the second lowest quartile of the serum bili-rubin level than that in the highest quartile of the serum bilirubin level (Q₂ vs. Q₄; HR, 9.36; 95% CI, 1.33 to 65.73; p = 0.024). In the normoalbuminuria subgroup (n = 236), multivariate analysis showed that the risk of developing CKD stage 3 or greater was higher in the lowest quartile of the serum bilirubin level than that in the highest quartile of the serum bilirubin level (Q1 vs. Q4; HR, 7.36; 95% CI, 1.24 to 35.82; p = 0.019). Conclusions: Serum bilirubin might be an early clinical marker for predicting the progression of CKD in patients with T₂DM and preserved renal function.

Mechanistic Insight into the Conversion Chemistry between Au-CuO Heterostructured Nanocrystals Confined inside SiO₂ Nanospheres

Jeon, Ki-Wan,Lee, Dong-Gyu,Kim, You Kyung,Baek, Kangkyun,Kim, Kimoon,Jin, Taewon,Shim, Ji Hoon,Park, Jeong Young,Lee, In Su American Chemical Society 2017 Chemistry of materials Vol.29 No.4

<P>By taking advantage of a nanospace-confined nanocrystal conversion protocol via high-temperature solid-state reaction within the SiO2 nanosphere, an in-depth study was conducted into the unique transformation behavior of the Au-CuO heterostructured nanocrystals (HNCs), which was discovered during the oxidative annealing of the embedded AuCu alloy nanocrystal (NC). The type of heterojuction structure of the oxidized AuCu NCs, between core(a)shell and heterodimer, could be determined by modulating either the annealing temperature (T-ann) or Cu contents (P-cu) in AuCu NCs; Au@CuO was generated only either at low temperature (T-ann <= 250 degrees C) or with very low Cu contents P-cu = 2.1), whereas the Au/CuO heterodimer was obtained as a major product in most of the cases at relatively high heat treatment (>250 degrees C). The systematic investigation of the conversion between HNCs could elucidate the distinct evolution pathway of the Au/CuO heterodimer via the kinetically accessed Au@CuO, revealing the escaping motion of the encapsulated Au core, which is more facilitated through a thicker CuO shell. This also demonstrated the high thermal stability of the Au@CuO with a very thin shell thickness due to the insufficient compressive lattice stain on the CuO shell to drive the morphological transformation into the heterodimer. Moreover, the higher operational stability could be detected for the Au@CuO with the lowest Cu content during catalytic CO oxidation, which correlates with its resistance against the thermal deformation.</P>