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식이 Xylooligo당의 난소화성과 담즙산 흡수 지연효과
주길재(Gil-Jae Joo),이인구(In-Koo Rhee),김성옥(Sung-Ok Kim),이순재(Soon-Jae Rhee) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.4
식이 xylooligo당의 생리적 기능성을 관찰하기 위하여 in vitro 실험에서의 xylooligo당의 난소화성과 담즙산 흡수 지연효과를 관찰하였고 in vivo 실험으로 정상 식이를 공급한 정상군(Normal)과 정상식이에 10% 올리고당을 공급한군(NX군)으로 나누고 다시 고콜레스테롤 식이내에 첨가한 xylooligo당 첨가 식이군(CX군)에서의 소장내 이당류 분해 효소활성을 관찰한 결과는 다음과 같다. 각종 올리고당의 분해 양상 조사에서 사람의 타액, 췌장액, 인공장액 및 대장애에서는 xylooligo당, fructooligo당, isomaltooligo당 등 모두 분해되지 않았다. 소장점액에서의 시간별 올리고당의 분해를 관찰한 결과 isomaltooligo당의 분해는 반응 4시간 후 대부분 분해되었으나 fructooligo당은 극소량 분해되었고 xylooligo당은 전혀 분해되지 않았다. 올리고당의 담즙산 흡수 지연 효과를 관찰한 결과 xylooligo당이 fructooligo당 및 isomaltooligo당에 비해 효과가 높았다. 동물실험에서 체중증가량은 정상식이에 10% xylooligo당 투여군(NX군)이 정상군에 비해 유의적으로 낮았다. 식이섭취량은 정상군에 비해 고콜레스테롤 식이군에서 유의적 차이가 없었다. 식이효율은 정상군에 비해 NX군과 CX군에서 유의적으로 낮았다. 간무게는 콜레스테롤 공급군에서 정상군보다 유의적으로 높았고, 신장무게는 식이군에 차이가 없었다. 소장무게는 정상식이에 10% xylooligo당 공급군(NX군)과 고콜레스테롤식이에 10% xylooligo당 공급군(CX군)에서 각각 비투여군에 비해 유의적으로 높았다. 맹장의 무게는 xylooligo당 비투여군인 C군을 제외한 실험군이 정상군에 비해 높았으며 CX군이 가장 높았다. 소장내 이당류분해 효소활성은 maltase의 활성이 lactase와 sucrase 활성보다 높았고 lactase, sucrase, maltase활성은 고콜레스테롤 식이에 xylooligo당을 첨가한 식이군(CX 군)이 xylooligo당 비투여군(C군)에 비해 유의적으로 낮았다. 이상의 in vitro 및 in vivo 실험결과를 종합해 볼 때 올리고당은 난소화성이며 담즙산의 흡수억제 및 이당류 분해 효소활성 억제작용이 있으며 그중에서 xylooligo당은 고콜레스테롤 식이로 인한 고지혈증을 억제시키는 지질 개선의 가능성과 당의 가수분해를 지연시켜 혈당조절의 가능성이 있다고 볼 수 있다. The digestibility of xylooligosaccharide(XO) by juices of the digestive tract and retardation effect of XO on the adsorption of bile acids were compared with fructooligosaccharide(FO) and isomaltooligosaccharide(IO). In vitro digestion experiments showed that any hydrolyzed products of FO, IO and XO were not detected by HPLC after reaction with saliva, pancreatic, artificial intesteinal, and large intestinal juices, and artificial sera for 4 hours at 37℃. However, IO were mostly digested by the small intestinal juice, and some quantity of FO were digested. XO were not digested at all by any enzyme of digestive tract. In order to investigate retardation effect of XO on the bile acid absorption. In vitro, permeability of bile acid against dialysis membrane was determined in the mixture of bile acid and XO. The per-meability of bile acid against dialysis membrane in control mixture which contained guar gum instead of XO was set 100%. The permeability of bile acid showed about 50% in the FO and IO mixture and 43% in the XO mixture. The activity of lactase in FO group and activity of sucrase and maltase in XO group in rat small intestinal mucosa were significantly decreased. Consequently, the present results indicate that XO is indigestible in digestive tract and has retarding effect of adsorption of bile acid compared with the other oligosaccharides. The disaccharidase activity of the XO dietary group was lower than that of the other oligosaccharides dietary group. Furthermore, it was suggested that hydrolysis of sugar may be retarded in digestive tract and glucose level in blood may be controlled effectively by the XO.
강병태(Byung Tae Kang),노동현(Dong Hyun Roh),주길재(Gil Jae Joo),이인구(In Koo Rhee) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.6
In order to investigate the function of xylA promoter(Pxyl) as regulatory region Pxyl-lacZ fusion gene was constructed by the insertion of xylA promoter to the multiple cloning site of upstream of lacZ gene in a multicopy numbered plasmid pMC1403 containing promoterless lac operon, which was designated pMCX 191, and Pxyl-lacZ fragment from pMCX191 was inserted to low copy numbered plasmid pLG339, designated pLGX191. The expressions of β-galactosidase in these recombinant plasmids containing Pxyl-lacZ fusion gene were induced strongly by the addition of xylose, repressed by the addition of 0.2% glucose in the presence of xylose. The catabolite repressions were derepressed by the addition of 1 mM cAMP as same as native xylA gene. The fragment of xylA promoter was partially deleted from the upstream of xylA promoter by exonuclease Ⅲ to investigate the regulation site of xylA promoter and the degrees of deletion derivatives of xylA promoter were analyzed by the DNA base sequencing. By the investigations of the induction by xylose, repression by glucose and derepression by CAMP on xylose isomerase production, the regulation site of xylA promoter may be located in segment between 165 and -59 by upstream from the initiation site of xylA translation.
Sphingomonas sp.224 균주에 의한 살균제 Tolclofos-methyl의 분해
곽윤영 ( Yun Young Kwak ),신갑식 ( Kab Sik Shin ),이상만 ( Sang Man Lee ),김장억 ( Jang Eok Kim ),이인구 ( In Koo Rhee ),신재호 ( Jae Ho Shin ) 한국환경농학회 2010 한국환경농학회지 Vol.29 No.4
In order to decrease level of an organopho-sphorus fungicide, tolclofos-methyl, from in situ ginseng cultivating soil, we isolated a tolclofos-methyl degrading bacteria from ginseng cultivating soil samples. The bacterial strain removed tolclofos-methyl around 95% after 3 days incubation with complete liquid media. The strain was identified as Sphingomonas sp. by 16S rDNA sequence comparison, and designated as Sphingomonas sp. 224. Through the GC-MS analysis, Sphingomonas sp. 224 was proposed to have an initiative degradation pathway generating the metabolite such as 2,6-dichloro-4-methyl phenol compound from tolclofos-methyl. In addition, Sphingomonas sp. 224 was confirmed representing the effective degrading capability to tolclofos-methyl in situ soil.