폐내 기관지 낭종

이석열,이숭진,박형주,이철세,이길노,김윤정,나주옥 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

A 33-year-old female was admitted to our hospital due to removal of lung mass which indicentally detected Chest radiography. Chest computerized tomography revealed lung parenchymal cystic lesion. Open thoracotomy was done for diagnosis and therapy. Cystic lesion was communicated to bronchus and wedge resection including cystic lesion was done. Bronchogenic cyst is congenital disease and mainly locate in mediastinum. It is rare in lung parenchyme. Herein we report a case of intrapulmonary bronchogenic cyst.

가토의 척수허혈에서 Aprotinin의 척수보호 연구

이석열,이길노,박형주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background : The complications of ischemic spinal cord injury on the descending and thoracoabdominal aorta remain a problem in spite of surgery and anesthesia advance. The protective effect of aprotinin was assessed from a rabbit spinal cord ischemia model in order to prevent ischemia reperfusion injury from the spinal cord. In the mechanism of the pathogenesis of ischemia-reperfusion injury, activated neutrophils are thought to be important in terms of their ability to produce various kinds of proteinase, which can degrade various proteins constructing human tissues. In addition, activation of blood protease cascades may contribute to celluar injury under this condition. Aprotinin is a serine-protease inhibitor and has been shown to inhibit activation of kallikrein-kinin system and plasmin fibrinolysis system. Aprotinin also preserve platelet function and partially inhibit neutrophil activation. such effect of aprotinin observed on ischemia-reperfusion injury of spinal cord after aortic clamping. Methods : In 24 rabbits, left vertical flank incision and retroperitoenal approach were done and ischemia was induced with clamping of the aorta just distal to left renal artery and proximal to aortic bifurcation for 20 min. Aprotinin was given 30,000 KIU/kg as a short intravenous injection after anesthesia, and was followed by 30,000 KIU/hr by continous infusion in study group(n=12). Similar volume of saline solution was used in control group(n=12). Physiological parameters were monitored in animals before aortic occlusion, during aortic occlusion, after aortic occlusion. Their neurological outcome was clinically evaluated up to 48hour postischemia. After 48 hour of the operation, all rabbits' victim were induced and their spinal cord, abdominal aorta, and its branches were processed for histopathological examination. Conclusion ; Mean aortic pressure, heart rate and arterial blood gas analysis showed no statistical difference between study group and control group. Bladder function also revealed no statistical difference. The average motor function score was significantly higher in study group than control group at 24 and 48 hour after the ischemic insult(p<0.05). Histological observations were revealed fewer ischemic damage in study group than control group. This results suggest that aprotinin reduces spinal cord injury and preserves neurologic function in transient spinal cord ischemia of rabbits.

알록산 투여로 유발된 실험적 당뇨병에 대한 사인추출물의 치료 효과

노혜원,이지나,구본선,조정임,박진우,김형로 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.2

연구배경:사인추출물이 알록산에 의한 당뇨 유발을 억제하는 효과가 있음을 이미 확인하였던 바 사인 추출물이 췌장 베타세포가 손상되어 유발된 당뇨병의 치료에 사용할 수 있는지의 가능성을 알아보았다. 방법:실험적 당뇨는 알록산(60㎎/㎏)을 생쥐 꼬리 정맥을 통해 주사하여 유발시켰다. 당뇨에 미치는 사인추출물의 영향을 관찰하기 위하여 사인추출물의 영향을 관찰하기 위하여 사인추출물(2.5㎎/생쥐)을 하루에 두 번 생쥐 복강 내로 투여하였다. 당뇨에 미치는 사인추출물의 영향을 관찰하기 위하여 생쥐에 사인추출물을 처리한 다음 혈당 및 혈청 인슐린 농도 변화 및 조직학적인 소견으로 확인하였다. 결과:생쥐에 알록산을 투여하면 췌장 베타세포가 파괴되어 고혈당 및 hypoinsulinemia가 유발되었다. 사인추출물을 알록산 투여 전 2일 동안 전처리하면 알록산에 의한 고혈당이 완전히 억제되었다. 뿐만 아니라 알록산에 의해 유발된 췌장 베타세포의 손상은 사인추출물의 투여로 현저히 개선되어 혈당 및 혈청 인슐린 농도가 거의 정상 수준으로 회복되었다. 알록산을 투여한 생쥐의 췌장 소도는 정상군에 비해 수가 현저히 감소되었을 뿐 아니라 남아있는 소도의 크기도 현저히 줄어들어 있었으나, 사인추출물을 처리하면 모두 회복되어 정상군과 유사한 양상을 보였다. 결론:사인추출물은 알록산에 의해 유발되는 당뇨병을 억제하는 방어 작용 뿐만 아니라, 알록산 투여로 이미 손상된 췌장 베타세포를 개선시킬 수 있는 치료 효과작용도 가지고 있음을 확인하였다. 이를 토대로 사인의 당뇨 개선 기전을 규명하면 당뇨병 치료제로서 이용할 수 있으리라 사료된다. Background : During the screening of natural products for potential antidiabetogenic components, a strong protective effect of Amomum xanthoides extract on alloxan-induced β-cell damage and in a mice diabetic model. In this study, the therapeutic effect of Amomum xanthoides extract was investigated after induction of diabetes by alloxan. Methods : Experimental diabetes was induced by the injection of alloxan(60mg/kg) to the mouse via the tail vein. To examine the effect the of Amomum xanthoides extract on diabetes, Amomum xanthoides extract (2.5mg/mouse) was admini-strated intraperitoneally. The effect of the Amomum xanthoides extract on alloxan-induced diabetes was observed by measuring the blood glucose and serum insulin level, and a histological examination. Results : Alloxan caused hyperglycemia and hypoinsulinemia by a selectively destroying pancreatic β-cell. Pretreating the with an Amoumum xanthoides extract completely protected them from the hyperglycemia induced by alloxan. In addition, the Amomum xanthoides extract administe 3 days after the of alloxan injection significantly abolished the hyperglycemia and hypoinsulinemia induced by alloxan. The alloxan-treated mice showed a marked change of in the pancreatic islets: the number of islets was reduced and the size of the remaining islets also decreased. However these effects of alloxan were significantly recovered by a later administration of the Amomum xanthoides extract. Conclusion : The amomum xanthoides extract contains potentially effective components, which both protect and treat alloxan-induced diabetes. The identification and action mechanism of the effective components of the Amomum xanthoides extract requires further investigation and it is suggested that the Amomum xanthoides extract be used as a therapeutic drug for diabetes(J Kor Diabetes 26:126~133, 2002).

기계부하를 고려한 GT 셀 형성방법

신동목,노형민,이종원 한국경영과학회 1989 한국경영과학회 학술대회논문집 Vol.- No.1

The purpose of this study is to develop Balanced Clustering (BC) algorithm for forming GT cells in manufacturing system. The BC algorithm considers the number of products per machine and production time as well as the kinds of machines. This consideration can relieve in some degree the bottleneck machine problem and the unbalance of machine load after clustering, which exist in current clustering algorithms. The BC algorithm is written in PASCAL and compared with another algorithm through an example.

급성 척수허혈 및 재관류손상에 대한 Na^+/H^+ 교환억제제(DMA)의 보호효과 : 가토 척수의 허혈 및 재관류손상 모형에 의한 실험적 연구 An Experimental Study in Spinal Cord Ischemia and Reperfusion Injury Model

고정관,박형주,이길노 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Spinal cord injury after operations on the descending thoracic and thoracoabdominal aorta remains a persistant clinical problem. Previous attemps to decrease the risk of this devastating complication by lowering the rate of metabloism of the spinal cord have met with varying success. We hypothesized that the tolerance of the spinal cord to an ischemic insult could be improved by means of Na^+/H^+ exchange inhibitor(DMA). DMA prevent intracellular Na^+ influx and maintain intracellular acid pH during ischemia by blocking the Na^+/H^+ exchange system. Additionally, subsequent Ca^++ influx during reperfusion is prevented by blocking the Na^+/Ca^++ exchange as well. It is well established that not only the intracellular acidosis during ischemia prolongs cellular survival but almost all of the reperfusion injury is associated with intracellular Ca^++ overload. Twenty four New zealnad white rabbits underwent 30 minutes of isolated infrarenal aortic occlusion after heparin anticoagulation. Clamps were placed both below the left renal vein and above the aortic bifurcation. In 12 rabbit(Amiloride Group), a bolus of amiloride(3㎎/㎏) intravenous injection and a bolus of diluted amiloride(100uM) was infused the isolated aortic segment immediately after crossclamping and immediately before release of clamping. In another control group(12 rabbits), the aortic segment was flushed with normothermic saline in a fashion identical to that of the study group. The aortic clamps were removed after 30 minutes, the abdomen was closed, and the animals were allowed to recover from anesthesia. Spinal cord function was assessed 72 hours after operation by the Talov's scale. All animals were put to death at 72 hours after operation and spinal cords were harvested for MDA and histologic analysis. The spinal cord function of all Amiloride group animals were fully intact with Talov's scores of 3-5; control group animals were all paraplegic with Talov's score of 0-2(p<0.001, student t-test). MDA level in Amiloride group was 0.046±0.06 and control group was 0.065±0.01(p<0.0001, student t-test). Histologic examination of spinal cords from Amiloride gropup rabbits revealed a little evidence of cord injury, whereas spinal cords from control group had evidence of extensive cord injuty with central gray necrosis, axonal swelling, dissolution of Nissl substance, and astrocyte and macrophage infiltration. Systemic and regional infusion of the crossclamped infrarenal rabbit aorta with Na^+/H^+ exchange inhibitor(DMA) nearly complete prevented paraplegia in our model despite a 30-minute ischemic insult.

抗生劑耐性傳達因子의 除去에 關한 實驗的 硏究

河大有,金炯魯,金圭次,李憲九 대한의학협회 1979 대한의학협회지 Vol.22 No.9

Secretion Pathway Engineering for Production of Kringle Protein LK8 and Invertase in Recombinant Saccharomyces cerevisiae

Hyung-Rho LEE,Yu-Mi KIM,Yi-Hyun BAE,Hyung-Kweon LIM,Yong-Cheol PARK,Jin-Ho SEO 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

1 . Assay of ornithine aminotransferase with ninhydrin

(Hyung Rho Kim),(Hye Won Rho),(Jin Woo Park),(Byung Hyun Park),(Jong Suk Kim),(Min Wha Lee) 전북대학교 의과학연구소 1995 全北醫大論文集 Vol.19 No.1

Intraspecific variations in macronutrient, amino acid, and fatty acid composition of mass-cultured Teleaulax amphioxeia (Cryptophyceae) strains

Lee, Bae Ik,Kim, Shin Kwon,Kim, Jong Hyeok,Kim, Hyung Seop,Kim, Jong Im,Shin, Woongghi,Rho, Jung-Rae,Yih, Wonho The Korean Society of Phycology 2019 ALGAE Vol.34 No.2

To compare the nutritional quality of TPG (Teleaulax / Plagioselmis / Geminigera) clade species of cryptomonads with that of RHO (Rhodomonas / Rhinomonas / Storeatula) clade species 6 Teleaulax amphioxeia (TA) and 1 Rhinomonas sp. strains were mass-cultured in newly designed 500-L photobioreactors to the end of exponential growth phase. Intraspecific variations (IVs) in terms of one standard deviation among the 6 TA strains in the compositions of the three macronutrients were 41.5 (protein), 89.8 (lipid), and 15.6% (carbohydrate) of the mean. When harvested from stationary growth phase mean compositions of essential amino acids (EAAs, 47.3%) and non-EAAs (52.7%) of the 2 TA strains, CR-MAL07 and CR-MAL08-2, were similar to those of a Chroomonas strain. The IVs between the 2 TA strains in the composition of EAAs (10.3 and 2.4) and non-EAAs (8.5 and 2.1% of the mean) were rather smaller than those of saturated fatty acids (30.3 and 26.1) and unsaturated fatty acids (UFAs, 12.0 and 12.5% of the mean) in f/2-Si and urea-based compound fertilizer (UCF) culture media, respectively. Mean compositions of eicosapentaenoic acid (EPA, 17.9%) and docosahexaenoic acid (DHA, 12.7%) of total fatty acids of the 2 TA strains were higher than those that of a Chroomonas strain. EPA and DHA compositions exhibited similar level of IVs between the 2 TA strains in f/2-Si (14.6 and 11.0) and UCF media (12.6 and 13.5% of the mean). Thus, the nutritional quality in terms of amino acids, UFAs, EPA, and DHA in a TPG clade species, T. amphioxeia was comparable to those of RHO clade species with notable IVs. Practically, biotechnological targets for TPG clade cryptomonad strains might be subspecies or clone level.

쿼터니언 오차모델과 등가오차모델의 비교

이형근(Hyung-Keun Lee),이장규(Jang-Gyu Lee),노용규(Young-Kyu Rho),박찬국(Chan-Gook Park) 한국항공우주학회 1997 韓國航空宇宙學會誌 Vol.25 No.2