Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

^27Al(p,α pn)핵반응에 의한 무담체 ^22Na 제법 연구

유국현,서용섭,양승대 東國大學校 1998 東國論叢 Vol.37 No.-

A method for the production of no-carrier added(NCA) ^22Na was developed via ^27Al(p, ㄷαpn) nuclear reaction with 50.5MeV protons. the half life of ^22Na is 2.6 years and main γ-energy is 1274.5keV and it is used standard source and sodium catabolism study. The cross-section and thick target yield for the reaction was measured in detail in the energy range of 50.5→20.2MeV in order to determine the optimum conditions for the production of ^22Na. the maximum cross-section for the production of ^22Na was 40.8 mbarn at 43.85MeV. the calculated production yield of ^22Na by 50.5MeV protons on aluminium was 60.9μCi/μAh. The seperation of ^22Na was carried out by ion exchange, precipitation and diffusion. It was found that ion exchange column operation using AG50W-X4 resin was the most efficient method among them.

MC-50 싸이클로트론을 이용한 Mn-54 제법 연구

유국현,서용섭 東國大學校 2000 東國論叢 Vol.39 No.-

The half-life of ^54Mn is 312.2 days and main γ-energy is 835keV, therefore it is used as a standard source for a multi-channel analyzer and a dose calibrator and managanese catabolism study in human body. In this paper, a method for the production of no carrier added ^54Mn was studied via ^54Cr(p,n) and ^59Co(p, αpn), with MC-50 cyclotron and described chemical isolation method of ^54Mn from a irradiated target by ion exchange resin. Chromium and cobalt were used as target materials. The production yields of ^54Mn by 50.5 MeV protons was 4.5 μCi.μ/Ah on chromium target and 11.85 μCi/μAh on cobalt target. The cross-section and thick target yield of ^59Co(p, αpn)^54Mn reaction were measured in the energy range of 50.5→22.2MeV in order to determine the optimum conditions for the production of ^54Mn. the maximum cross-section was 47.48 mbarn at 41.2MeV.

절인반경차이에 따른 연질재료의 정밀가공 특성 연구

유기현,권용기 韓國工作機械學會 2000 한국생산제조학회지 Vol.9 No.1

This paper deals with the precision cutting characteristics of mono-crystal diamonds, poly-crystal diamonds and tungsten carbide tool on ductile material. The cutting tests were carried out under various uncut chip areas and 20㎛ depth of engagement. The machinability in precision machining was discussed from the viewpoints of the normal cutting forces and the surface roughness of the workpiece. As the feed rate decreases, the normal force difference for cutting edge radii appears to be large. In various cutting edge radii, the surface roughness difference when cut the copper which is ductile material than the aluminium alloy is large. As the same cutting condition, the hardness value on cut surface with the diamond tool appears to be smaller than that of the tungsten carbide tool.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

제주 신선당근의 대일 수출경쟁력에 관한 경제분석

현공남,유영봉 濟州大學校 地域社會發展硏究所 1995 社會發展硏究 Vol.11 No.-

응용을 위한 ISFET의 측정회로

유상대,신장규,이용현,이종현 慶北大學校 1983 論文集 Vol.36 No.-

The ion-sensitive field-effect transistor (ISFET) is one of the family of chemically sensitive semiconductor devices(CSSD) which has been the object of considerable research and development. This study describes the operation of an ISFET as an electronic device. The measurement circuit based on this operation theory is developed for an appropriate ISFET application.

심장사상충증 이환견의 치료 1례

심재현,김유수,김건우,이병희,김지용,박성준,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 4-years old pointer dog was referred to the Veterinary Teaching Hospital of Chungnam National University with chief compliment of anorexia, exercise intolerance, hemoptysis, coughing and ascites. It was diagnosed as heartworm disease (class 3) by physical and laboratory examination, radiography, ultrasonography and electrocardiography. This dog was undertaken by adulticidal therapy. Normal conditions and good appetite after therapy were observed.

마이크로 프레스 구동을 위한 기어시스템 개발

홍용,김현식,고유곤,홍동표,김성욱 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

In this paper, gear system for Micro-press actuator parts is developed. The micro-press system consists of AC geared servo motor, gear system and high precision ball screw. Herein, the micro-press gear system not only forms the micro thin foil valve but also forms the hydro dynamic bearing for a high precision rotational equipment. we analyzed the transmitted power capacity of gear system and torque, angular velocity of ball screw, because we are to select the appropriate servo motor and then we apply result of analysis to the micro press system. For the next step, we plan to study for the development of forming speed for a productivity with high precision.

國內藥草의 抗癌成分硏究 (1) : 馬兜鈴, 石蒜, 三稜, 無花果皮, 碧梧桐皮, 人參 등 70餘種에 對하여

丁明鉉,李敦日,金永洙,朱興珪,劉永鐘,金成鎬,鄭鐘南 朝鮮大學校 1977 綜合論文集 Vol.1977 No.-

Alcohol, water, ether, and petroleum ether extracts of 75 species Korean medicinal plants have been tested for their antitumor activity. Most of the extracts had slight activity only. Some extracts had more pronounced acitivity. For antitumor activity, ddD-Mice of weighing 18-25g were used. 0.2 ml of cell suspension in 0.9% saline-glucose solution containing 10?? ascites tumor cells of either Sarcoma 180, Ehrlich carcinoma was transplanted intraperitoneally into each mouse. The control group animals in a given experiment consisted of 14 mices and the experimental group, 7 mices. The materials to be tested were administered once a day by intraperitoneal injection, commencing the day after tumor transplantation until the test animal died, and then the control group was received only 0.5% carboxy methyl cellulose (CMC). The mean survival time of each group was calculated according to the formula indicated in the protocols of the Cancer Chemotherapy National Service Center. In the antitumor activity screening, extracts had pronounced greater prolongation of survival time (50%-100%) were Aristo-lochia contorta, Lycoris radiata, Scirpus maritimus, Ficus carica, Firmiana platanifolia, Panax ginseng, Piper nigrum among the 75 species. The antitumor screening result were tabulated in Table II.