출생후 흰쥐 구치의 발육에 따른 치수의 신경지배 발달에 관한 면역조직화학적 연구

김영진,남순현,배용철,김동길,김현정 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.4

The purpose of this study was to investigate the postnatal development of pulpal innervation with the tooth development and eruption process in developing rat mandibular molars (postnatal 1, 5, 10, 15, 20, 25, 35day-old and adult rats). Immunohistochemical method was carried out for the detection of nerve fibers, using the antibody against calcitonin gene-related peptide(CGRP). The results were as follows: The feature of CGRP-IR nerve fibers were shown in a bead-like appearance. The time of nerve entering into the dental papilla of tooth follicle began at the occured advanced dentinogenesis. The development of Raschkow plexus began at the formative stage of the roots and was accelerated at the stage of the crown emerged into the oral cavity. The number of nerve fibers entering the odontoblastic layer increased with the tooth eruption and mastication. The development of innervation was shown to be related with the stage of the development of individual teeth rather than the chronological age of the rat.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.

국내 주요 호수의 육수학적 조사(4): 주암호

김범철,박주현,허우명,임병진,황길순,최광순,최종수 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

In this study limnological characteristics of Lake Juam was surveyed from June 1993 to May 1994 in order to provides important information regarding water resources. Seschi disc transparency, epilimnetic chlorophyll a(chl-a), tatal nitrogen(TN), total phosphorus(TP) concentration and primary productivity were in the range of 2.0~4.5 m, 0.9~13.6 mgChl/㎥, 0.78~2.32N/l, 11~56 mgP/㎥, 270~2,160mgCm^(-2)day^(-1), respectively. On the basis of TP, Chl-a and Secchi disc depth, the trophic state of Lake Juam can be classified as mesotrophic lake. The phosphorus inputs from non-point sources are concentrated in heavy rain episodes during the monsoon season. As a result, phosphorus concentration are higher in summer than in winter. TP loading from the watershed were estimated to be 0.9 gPm^(-2)yr(-1), which correspond to a boundary of the critical loading (1.0 gPm^(-2)yr(-1), which correspond to a boundary of the critical loading (1.0 gPm^(-2)yr(-1)) for eutrophication. From the results of the algal assay, both phosphous and nitrogen act as limiting nutrients in algal growth. The seasonal succession of phytoplankton community structure in Lake Juam was similar to that observed in other temperate lakes. Diatoms(Asterionella formosa and Aulacoseira granulata var. angustissima) were dominant in spring and winter, cyanobacteria (Microcystis aeruginosa, M. sp. and M. viridis) were dominant in warm season. The organic carbon, nitrogen and phosphorus content of lake sediment were 9.5~14.0 mgC/g, 1.01~1.82 mgN/g, and 0.51~0.65 mgP/g, respectively. The allochthonous organic carbon loading from the watershed and autochthonous organic carbon loading by primary production of phytoplankton were determined to be 1,122 tC/yr and 6,718 tC/yr, respectively. To prevent eutrophication of Lake Juam, nutrient management of watershed should be focus on reduction of fertilizer application, proper treatment of manure, and conservation of topsoil as well as point source.

견과류에서 Ganglioside 분석

김현수,전길자 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-

소수성인 지방 부분과 친수성인 당 부분을 가진 양쪽성 물질로서 N-acetylneuraminic acid를 포함하는 ganglioside가 견과류에 존재하는지를 확인하기 위해서 잣, 호도, 살구씨, 아몬드로부터 Folch-Suzuki방법으로 당지질을 분리하였다. 또한 periodate-resorcinol 방법으로 N-acetylneuraminic acid를 정량한 결과 각각 10당 잣은 0.013㎛ol, 호도는 0.015㎛ol,살구씨는 0.05㎛ol, 아몬드는 0.015㎛을 포함하고 있었다. 견과류에서 추출해낸 당지질을 HPTLC로 분석한 결과 다양한 당지질의 존재를 확인할 수 있었다. 그 중 잣과 살구씨에서 분리한 당지질을 DEAE-Sephadex A-25 column chromatography를 수행한 결과 잣에서는 여러 종류의 중성 당지질과 monosialoganglioside를, 살구씨에서는 여러 종류의 중성 당지질과 disialoganglioside를 분리할 수 있었다. 본 연구결과 식물에도 ganglioside가 존재하는 것을 밝히게 되었다. The compositions of gangliosides in Pinus koraiensis S. et Z.(pine nut), Juglans sinensis DODE (walnut), Armeniaca semen (apricot seed), and Prunus dulicis D.A. Webb(almond) were investigated by Folch partition, HPTLC. DEAE Sephadex A-25 column chromatography. Total polar glycolipid was extracted in aqueous phase by Folch-Susuki method. Extracted glycolipids were separated on HPTLC glass plate in the solvent system(chloroforn: methanol: water containing 0.02% CaCl_2 ? 2H_2O=55:45:10,v/v/v). Glycolipids including ganglioside were visualized with resorcinol reagent or orcinol-ferric chloride-sulfuric acid reagent. The amount of total N-acetylneuraminic acid was determined using the periodate-resorcinol method. Pinus koreaiensis S. et Z.,Juglans sinensis DODE, Armeniaca semen, and Prunus dulicis D. A. Webb contained 0.013,0.015,0.050, 0.010㎛ol of N-acetylneuraminic acid per 10g samples respectively. Glycolipids extracted from pine nuts 100g and apricot seeds 100g were analyzed on DEAE Sephadex A-25 column (acetate form, 1.3×14㎝). The gangliosides were eluted with continuous gradient of ammonium acetate in methanol (0 to 0.6M) and our results suggest that monosialoganglioside exists in pine nuts and disialoganglioside exists in apricot nuts.

Cyclosporin A의 肝毒性에 미치는 右歸飮의 影響

金昊顯,申興默,金吉萱 동국대학교 한의학연구소 1993 東國韓醫學硏究所論文集 Vol.2 No.2

The purpose of this study is to observe the effect of Woogyuyem on serum reaction and hepatic tissue in Cyclosporin A treated rats. In this study, the experimental rats divided two group(Contro1 group and Sample group). Under the Same condition, both control group and sample group were injected to abdomen with Cyclosporin A for 7days. And then, control group were administered water, sample group were administered Woogy-uyeum for 30days. The change of transaminase and bile acid and SOD activity in blood serum, hepatic tissue were measured at 3rd, 7th, 15th, 30th day. The obtained results are surnmarised as follows : 1. In the change of SGOT contents, as compared with control group, sample group was significantly decreased at 7th and 15th day. 2. In the change of SGPT contents, as compared with control group, sample group was signi�icantly decreased at 15th day. 3. In the change of serum bile acid contents, as compared with control group, sample group was significantly decreased at 7th and 15th day. 4. In the change of serum SOD activity, as compared with control group, sample group was significantly increased at 15th and 30th day. 5. In the change of hepatic tissue, as compared with control group, the vacuolation of hepatocytes in the sample group was reduced, the liver plates were reconstructed, and the epitherial cell wasn't destructed.

미국의 식물유래약용물질(Plant-Made Pharmaceuticals, PMPs)의 안전성 규제

김태산,원소윤,이근표,류태훈,진용문,이길복,김현준 한국국제농업개발학회 2005 韓國國際農業開發學會誌 Vol.17 No.1

1. 식물유래 약용물질 (PMP)은 살아있는 식물에서 생산된 치료용 단백질로, 발효조에서 세포계통을 이용하는 것보다 적은 비용으로 높은 순도의 단백질을 생산할 수 있어 이에 대한 연구개발이 확대될 전망이다. 2. 식물유래 약용물질의 재배시험을 감독하는 주관부서는 미농무부(USDA)의 동식물 검역청(APHIS)이다. 3. PMP의 생산에는 알팔파. 옥수수, 벼 잇꽃과 담배 등이 이용되고 있다. 4. 현재 상업화된 PMP는 없으나 ProdiGene, Inc사 등 몇몇 생명공학 회사에서 이들의 연구개발이 진행되고 있다. 5. 2002년에 PMP 재배시험은 130에이커 규모로 34개 시험장에서 20건이 허가 되었으며 주별로는 애리조나, 네브라스카 등 14주에서 시험이 이루어 졌다. 6. 미국 USDA의 PMP 재배 지침에 의하면 PMP의 재배생산에는 안전성확보를 위한 별도의 전용장비를 필요로 하며 파종에서 생산까지 엄격하게 관리된다. 7. 미국 USDA의 APHIS는 2002년 PMP 옥수수를 생산하는 ProdiGene, Inc사에 대하여 2건의 포장시험허가 위반사항을 적발하여 벌금 조치를 한바있다. Plant-made pharmaceuticals(PMPs) are therapeutic agents(pharmaceutical proteins) produced in genetically engineered plants. To yield these valuable products plants offer several advantages that include large-scale production capacity at a reduced cost, equivalent purity/activity to other manufacturing systems, and freedom from contamination with animal pathogens, prions, or disease-causing germs. But there are risks of contamination of the food supply and unintended effects on ecosystems. In the U.S. PMPs are regulated under stringent requirement of the U.S. Department of Agriculture(USDA) and the Food and Drug Administration(FDA). In this article, contamination incident with PMP corn engineered by ProdiGene, Inc will be briefly introduced and regulations of PMPs will be discussed.

부상형 직파육묘방법에서 cell 크기에 따른 dibbling의 깊이가 담배 묘 생육에 미치는 영향

김영신,신주식,백기현,배길관 충북대학교 엽연초연구소 2001 煙草硏究 Vol.18 No.-

The objects of this study were to search ideal dibbling depth and cell size in direct-seeding improved float system. Percentage of dry cell was high in dibbling depth of 0.0mm and 7.5mm in every cell size. Dibbling treatment increased the seed germination rate. Germination power of the 128 cell was high in dibbling depth of 15.0mm or more, and that of the 162 cell and 200 cell was high in the depth of 12.5mm or more. Rate of usable plant of the 128 cell was high in dibbling depth of 15.0mm, and that of the 162 cell and 200 cell was high in the depth of 12.5mm. Compaction and low oxygen levels of media by deeper dibbling depth contribute significantly to spiral root. According to the interacting results of the treatments, dibbling depth of 15mm in 128 cell and that of 12.5mm in 162 cell and 200 cell were recommended to get high seed germination rate and good seedling growth.

진동시험에 의한 참외의 진동특성

김만수,정현모,김기석,박정길 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

During a long journey of agricultural products from the production area to markets, the quality of agricultural products was always affected by some degree of vibration. The vibration input during the transportation may cause serious agricultural product injury, and this damage is particularly severe whenever the vegetable inside package is free to bounce, and is vibrated at its resonant frequency. The objectives of this study were to determine the resonant frequency of the oriental melon and to investigate the relationships between resonant frequency and physical properties of the oriental melon such as mass, volume and major and minor axes. In this study vibration testing device was constructed to determine the vibration response of the oriental melon in frequency ranges of 5 to 150 Hz. The computer program for controlling the vibration shaker and the function generator and measuring the vibration characteristics of the oriental melon was developed. The ranges of resonant frequency and peak acceleration at resonance of the oriental melon were 51 to 73 Hz and 1.24 to 1.92 G-rms, respectively. The resonant frequency and the peak acceleration decreased with the increase of the sample mass, volume, major and minor axes of the oriental melon. Multiple regression models for resonant frequency and peak acceleration of the oriental melon as a function of mass, major axis and minor axis of the sample were developed and analyzed.

성인 물질남용 환자의 치료결과와 관련된 요인

김길숙,박용천,남정현,안동현,오동열 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 본 연구는 성인 물질남용 환자의 치료프로그램 탈락율, 치료결과 및 예후에 영향을 미치는 요인을 알아보기 위해 시행되었다. 방 법 : 1992년 9월 1일부터 1997년 4월 15일까지 국립서울정신병원 물질남용 입원치료프로그램에서 치료를 받고 퇴원한 환자 97명을 대상으로 병록지 검토를 통해 인구학적 특징, 물질남용상의 특징, 입원전 위험 행동력, 치료기간중의 특징이 치료완료여부와 관련이 있는지 분석해 보았고, 이중 전화추적조사가 가능했던 53명을 대상으로 물질남용 중단여부를 조사하고, 이와 관련된 요인을 알아보았다. 결 과 : 1) 퇴원형태에 따라 59명의 탈락군(60.8%)과 38명의 완료군(39.2%)으로 나누어 비교한 결과 탈락군은 입원기간이 짧고, 자의 입원율이 낮으며, 난폭행동의 과거력이 많고, 규칙위반의 횟수가 많은 것으로 나타났다(p〈.05), 프로그램에서 탈락한 주 이유는 병동내 규칙위반(45.3%), 산책중 도망(23.7%), 외출, 외박중 미귀원(15.2%)의 순이었다. 2) 전화로 추적조사가 가능했던 53명중 32명이 물질남용을 중단했고(60.4%). 21명이 아직 남용중인 것으로(39.6%) 나타났다. 남용군은 공존정신질환의 빈도가 높고, 과거 정신과적 입원 횟수가 많으며, 자살기도력의 빈도가 높은 것으로 나타났다(p〈.05). 3) 물질남용을 중단하게 된 계기는 국립서울정신병원의 치료프로그램에 참여하고 난 후가 45.3%로 가장 많았고, 치료를 완료한 환자일수록 퇴원후 치료결과가 좋은 것으로 나타났다. 그리고 물질남용을 중단한 환자일수록 직업을 가지고 있는 경우가 더 많았다(p〈.05). 결 론 : 물질남용 여부는 공존정신질환, 과거정신과적 입원횟수, 자살기도력등 동반된 정신과적 문제와 관련이 많은 것으로 나타나 이런 요인들에 대한 철저한 평가 및 치료가 필요할 것으로 추측되고, 환자의 동기는 치료를 완료하는 중요한 요인으로 나타나 입원 과정에서 치료에 대한 동기를 강화시킬 수 있는 정신과적 면담이나 전략이 필요할 것으로 생각된다. Objectives : We investigated the factors related to the treatment outcome for substance abuse inpatients. Methods : We reviewed the medical records of 97 patients who were discharged from the substance abuse treatment unit in Seoul National Mental Hospital. We then had follow-up telephone interview with 53 family members and / or patents of 97 patients. Results : 1) Among 97 discharged patients, 59 patients were dropped out, 38 patients graduated successfully from the treatment program. The dropout group had shorter treatment period, were more often admitted involuntarily, had greater incidence of violence history and violated ward rules more often(P<0.5). The main reasons for dropout were violation of rules(45.3%), escape during outdoor activities(3.7%), and refusal of return to the hospital(15.2%). 2) At the follow-up interview, 32 patients were abstaining from substances whereas 21 patients resumed use. The abuser group had more previous psychiatric admissions, more comorbid psychiatric disorders, and higher percentage of history of suicide attempts than the abstaining group(P<0.5). 3) After the discharge from the hospital, 45% of patients showed to discontinue substance. Treatment graduates were more in recovery from substance abuse. The abstaining group had a higher percentage of employment than the abuser group(P<0.5). Conclusion : The treatment outcome was more likely to be related with co-existing psychiatric problems such as previous psychiatric admission, comorbid psychiatric disorders, and history of suicide attempts. Therefore it is suggested that careful evaluation and appropriate treatment for such factors are needed for better treatment outcome. And psychiatric interview to enhance the motivation of patients is needed for completion of treatment.

殺蟲性 結晶蛋白質 特異的인 抗體의 生産

김기윤,이정민,유형진,백길현,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Bacillus thuringiensis is a gram-positive soil bacterium characterized by its ability to produce crystalline inclusions during sporulation. These parasporal bodies consist of protoxins known as crystal proteins exhibiting highly specific insecticidal activities. The Lepidoptera-specific protoxin, Cry IAc, was purified from E.coli JM103 harboring the cry IAc gene. The gene was isolated from Bacillus thuringiensis kurstaki HD-73 and subcloned into an expression vector, pKK223-3. The recombinant DNA(pOS4201) was transformed into E.coli JM103. The Cry I Ac protoxin overexpressed in the E.coli was isolated from the total proteins by differential solubility. The protoxin were trypsinized to obtain activated toxin. The proteins was resolved on SDS gel. The molecular weights of the pro-and toxin were resistered about 130,000 and 65,000 Daltons respectively. The Cry IAc toxin was used as immunogen to produce anti-Cry IAc antiserum in a rabbit. The immunogen (200㎍/ 200㎕) was mixed with an equal volume of Freund's complete adjuvant, and the mixture was hypodermically injected to the hide back (30 spots) and paws (2 spots) of the rabbit. The immunization was performed four times every two weeks. The dosage of the immunogen was decreased by half, and incomplete adjuvant was substituted for the complete one from the second immunization. The serum was prepared from the blood as described elsewhere. Immunoreactivity of the serum was examined by dot-blotting with the aid of GAR-HRP indirect immunoassay kit. It has been found that the anti-Cry I Ac antiserum recognized nano-gram quantity of antigen under these experimental conditions. The antiserum will he used for the development of immunochemical mean(s) to screen transgenic plants transformed by the insecticidal characteristics of Cry I Ac.