녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

행우서옥본(杏雨書屋本)『황제내경태소(黃帝內經太素)』 권(卷)21, 권(卷)27의 출간(出刊) 의의(意義)와 그 내용에 대한 고찰(考察)

김종현 ( Jong Hyun Kim ),백유상 ( You Sang Baik ),장우창 ( Woo Chang Jang ),정창현 ( Chang Hyun Jeong ) 대한한의학원전학회(구 대한원전의사학회) 2011 대한한의학원전학회지 Vol.24 No.5

『Hwangjenaegyeong-taeso(黃帝內經太素)』 is a classic work of Yang Sang-seon(楊上善), which comprises original articles of 『Hwangjenaegyeong(黃帝內經)』 along with 『Somun(素問)』, 『Yeongchu (靈樞)』, and 『gapeul(甲乙)』, as a one of the oldest annotated publications. Therefore, its significance lies in that 『Hwangjenaegyeong-taeso(黃帝內經太素)』 is a valuable work to reconstruct the original text of 『Hwangjenaegyeong(黃帝內經)』 and comprehend its fundamental ideas. The only printed edition of 『Hwangjenaegyeong-taeso(黃帝內經太素)』 was photocopied in 1981, and is currently known as ``Orient Edition``. While ``Orient Edition`` was referred to as the draft for the latest revised edition, volume 21 and 27 were photocopied from hand-copied edition, not the original. The original publications of ``Orient Edition`` have been stocked at ``Haengwuseook(杏雨書屋)`` of Japan and were recently published. Hence, a comparative study between the two original volumes and the former ones has been conducted. Although the most of the differences were trivial, some may have led to distorted interpretation of the text. The errors of the former revised edition fall into a few specific categories, and the most significant ones were errors that were made during the hand-copying procedure. Moreover, there were errors that were made due to the low resolution of the former draft, and simple errors during the publishing. In this work, examples of such cases were presented, and the results were collected.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

평면 2자유도 모델을 이용한 리니어 모터로 구동되는 다축 마이크로 밀링머신 크로스헤드의 진동해석

장성현,황현영,정용민,최영휴,홍정표 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

In this paper, a simple 2-D.O.F. planar motion model is proposed in order to analyze the snake motion of a cross head assembly of a Multi-axis micro milling machine (M4) for BT-factory, that is travelling on linear guide. In the proposed mathematical model, the friction between head and guide ways is neglected, and also the support structures including the linear guides of M4 are assumed to be rigid. The equations of motion of the proposed model are derived and successfully solved to determine vibration responses of a cross head assembly due to some applied traction forces.

위 MALT 림프종에서 다발성 림프절 전이로 오인된 동시성 림프절 결핵 1예

김성은,김규종,김도현,송준영,문대성,장리라,유찬희,문원,박무인,박선자,김영옥 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

28세 남자가 내시경적 점막절제술을 통해 저등급 위MALT 림프종으로 진단되었다. 병기설정을 위해 18F-FDG PET 검사를 시행하였고 우측 쇄골상부, 우측상/하부 기관주위, AP window 부위에 강한 섭취가 관찰되어 전이성 림프절로 판단하였다. 그러나, 우측 쇄골상부 림프절에서 시행한 절제생검에서 만성육아종성염증소견이 관찰되었고 조직의 결핵 중합효소연쇄반응에서도 양성 반응을 보였다. 2개월간 항결핵제의 사용 후 시행한 추적 컴퓨터단층촬영 검사에서 림프절 크기의 감소를 보여 최종적으로 위 MALT 림프종에서 광범위 림프절 전이로 오인된 동시성 림프절 결핵으로 진단되었다. A 28-year-old man was diagnosed as gastric MALT lymphoma in diagnostic EMR (endoscopic mucosal resection). He subsequently underwent an 18F-FDG PET. 18F-FDG PET showed intense multifocal uptake in right supraclavicular, right upper and lower paratracheal, and AP window areas that was considered radiologically to represent widespread metastatic lymphadenopathy. Excisional biopsy of a supraclavicular lymph node, however, revealed chronic granulomatous lymphadenitis with a positive reaction of Tb-nested PCR. After antituberculosis treatment for 2 months, a follow-up CT scan showed complete resolution of the lesions. Intense 18F-FDG uptake could be due to an infectious process such as synchronous tuberculous lymphadenitis. Therefore, this could mimic a malignant condition such as lymphoma with extensive lymph node metastasis due to a false-positive finding, which may lead to a misdiagnosis.

강관 보강된 전단벽 경계부재의 전단성능에 관한 실험연구

성수용,윤현도,정수영,장민수 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

The purpose of this study is to investigate the begavior of concrete filled square stee ltubular boundary elements subjected to cyclic shearing force and constant axial force. Six boundary elements, with a/D=1.0,were tested to determine their maximum shear force and compare primary curve, energy absorption capacity and stiffness degradation. As a result, in the both high and normal strength concrete boundary elements, the larger the wall breadth to wall thickness ratio(D/t) of the steel tube,the more prominent the decrease in structural performance due to cyclic loading. In the boundary element with D/t=25, the crushing of tube concrete were accompanied by considerable lateral displacement,while in the boundary element with D/t more than 39, the lacal bucking of steel tube and the crushing concrete were accompanied by considerable axial shortening.

Phytosphingosine의 protection 화학 및 이 유도체들의 반응성에 관한 연구

장소현,남궁성건 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

To develop synthetic routes for the target molecule, (2S,3R,4R)-2-aminooctadecane-1,3,4-triol (1), three kinds of syntheses for key intermediates (2a), (4) and (5) were explored from phytosphigosine through several protection processes. The first approach was proceeded by the reaction of oxazoline (2) with acetone, producing bicyclic compound (3) in 70% yield, instead of acetal (2a). Formation of the compound (3) was unavoidable in Lewis acid-induced acetal protection reactions, as a major product. The second key intermediate (4) was prepared from the reaction of oxazoline (2) with CH2Br2 under basic phase transfer reaction condition in 60% yield. Methylene acetal (4) was not reactive with strong bases so that it was not converted into the desired isomer (4a) at all. Finally, synthesis of another key intermediates (5) was successfully achieved by the reaction of oxazoline (2) with diethyl carbonate in 83% yield. The lithium hexamethyldisilazide (LHMDS)-promoted reaction of carbonate (5) only gave tricyclic compound (6) in 83% yield, without formation of the precursor (5a). The proposed formation mechanism for compound (6) is described.

304L 스테인레스강의 변태유기 소성에 미치는 변형온도의 영향

성장현,강창룡 동아대학교 공과대학 부설 한국자원개발연구소 1992 硏究報告 Vol.16 No.1

The effect of deformation temperature on transformation induce plasticity in 304L stainless steel with metastable austenitic phase investigaged. The result obtained from this study are summarized as follows. Tensile properties of the metastable austenitic 304L stainless steel remarkably influenced by deformation temperature and mainly controlled by transformation induced martensite. Tensile strength increased with decreasing deformation temperature and the elongation showed maximum value near 40℃ With lowering the deformation temperature, the volume fration of transformation induced martensite increased at the same strain. ε-martensite was formed at the begining stage of deformation in the low temperature region of low stability of austenite. The volume fration of ε-martensite increased with increasing strain and decreased after showing the maximum value. The maxium quantity of ε-martensite was obtained at low strain with decreasing deformation temperature.

복합조직강의 열처리경로가 조직학적인자 및 기계적성질에 미치는 영향

성장현 東亞大學校 海洋資源硏究所 1988 硏究論文集 Vol.1 No.1

The effect of changes in heat treatment paths and the addition of alloying elements on the formation of ferrite and austenite in dual phase steel heat treated in the two phase field of both ferrite and austenite were investigated. For this investigation Fe-C-Mn-Cr and Fe-C-Mn-Mo alloys were prepared throughout the experiment. The results obtained from this study are summarized as follows. The growth rate of ferrite obtained during isothermal heat treatment in the range of ferrite plus austenite directly from austenite single phase region decreased with the addition substitutional alloying elements such as Mn, Cr and Mo. This phenomena were remarkable with increasing temperatures of isothermal heat treatment. The carbide particles exsisted in the matrix of ferrite became a source of carbon supply at the austenites which were in the vicinity of grain boundary of ferrite. The easist diffusion path of carbon atom from ferrite matrix to austenite was the dislocations formed near to the ferrite due to the continuous growth of austenite. The volume fraction of martensite for the specimens isothermally heat treated after austenitization was shown to be remarkably different compared to that for the specimens obtained by using intercritical annealing, depending on the addition of Cr and Mo. The substitutional alloying elements involved were considered to become more effective to inhibit the formation of ferrite than to accerate the austenite formation. During intercritical annealing of the steel which has the initial structure of ferrite and carbide, tensile strength increased and yield strength became lowered with the formation of austenite at the grain boundaries of ferrite and simultaneously dissolution of carbide. Yield strength and elongation became minimum and maximum respectively after remarkable dissolution of pearlite and carbides.