Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

고관절 전치환술 후 합병된 비구 컵 해리와 동반된 활액낭종 : 증례보고 Case Report

성일보,정국진,박수철,김진호,정형진,황재광,염재광,정병현,안종국 대한고관절학회 2001 Hip and Pelvis Vol.13 No.1

1차 의료기관에서 코감기에 처방한 향균제

정형준,이훈재,김은실,이진수,정문현 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

Background : Upper respiratory infections (URIs) are common benign illnesses that are associated with significant economic burden, adverse effects of medications, and antimicrobial resistance. These effects have been evaluated chiefly at the tertiary health care centers. The purpose of this study was to investigate the prescribing habits, including antibiotic use, for the management of URIs among the primary care physicians. Methods : Between July and August 2006, 23 medical students visited 122 clinics of primary care physicians for the management of simulated common cold. They were instructed to present symptoms that simulated common cold; clear rhinorrhea and nasal stuffiness that lasted for 3 days, and neither was accompanied with fever nor myalgia. Results : The physicians' specialties were as follows: 43 were general internists; 40, otolaryngologists; 23, general practitioners; 15, family practitioners; and 1, pediatrician. The mean number of prescribed oral medications was 4.71 (S.D.±0.951). Glucocorticoids were prescribed in 10 (8.2%) clinics. Antibiotics were prescribed in 50 (41.0%) clinics - cephalosporins in 17, amoxicillin-clavulanate in 16, amoxicillin in 8, fluoroquinolones in 5, macrolides in 3, and trimethoprim-sulfamethoxazole in 1 clinic. There was no difference in the rates of antibiotic prescription among the specialties. Injections were recommended in 32 (26.4%) clinics. With the exception of chest X-ray recommended in 1 clinic, no laboratory or radiologic evaluation was performed. Conclusion : The antibiotic prescription rate observed in this study is lower than those of the previous studies. Since diagnostic tests are seldom performed, diagnostic ambiguity might be a key cause for the overuse of antibiotics in the management of URI. The reasons for the high rates of prescription of parenteral medications need to be evaluated. 목적 : 상기도 감염은 매우 흔한 질환으로 개개인으로 보면 중요하지 않은 병이나 국가 전체로 보면 비용, 부작용, 항균제 내성에 미치는 영향이 더 문제가 되는 병이다. 이런 영향에 대한 연구가 주로 3차 병원에서만 되므로, 3차 병원에 도착하기 전까지 받은 치료 내역이 연구 결과에 큰 영향을 미칠 수 있지만, 국내에서는 의약분업 후로는 의원에서 감기 처방에 대한 조사가 없어 현재 진료 상황을 알 수가 없다. 이에 의약분업 이후, 1차 의료에 종사하는 의원들을 대상으로 코감기에 관련된 진단의 다양성, 주사제의 사용, 항균제 사용에 대해 조사를 하였다. 재료 및 방법 : 2006년 7월과 8월에 걸쳐 서울, 경기, 인천에 개업하고 있는 일반의, 내과의, 이비인후과의, 가정의를 대상으로 122개 의원을 조사 하였다. 23명의 의과대학생들이 코감기 환자로 모의를 하여 개원의들의 진찰을 받은 후, 진단과 처방을 분석하였다. 코감기 증상은, 3일간 지속한 콧물과 코막힘을 주소로 했고, 발열과 오한은 없었으며, 목아픔과 기침은 약간 있는 정도로 하였다. 결과 : 조사한 의원에서 코감기에 사용한 경구 약제 수는 평균 4.71 (SD±0.951), 과별 차이는 없었다(P=0.99 by chi-square test). 부신피질호르몬은 10개(8.2%) 의원에서 처방하였다. 항균제는 50개(41.0%) 의원에서 처방하였으며, 세팔로스포린 17건, Augmentin 16건, amoxicillin (7) 또는 bacampicillin (1) 8건, 퀴놀론 5건, macrolide 3건, trimethoprim-sulfamethoxazole 1건이 었다. 과별로는 내과 34.9%, 이비인후과 52.5%, 가정의학과 33.3%, 일반외과 39.1%, 소아과 0%였고 통계적 의미는 없었다(P=0.41 by chi-square test). 주사를 권한 경우는 32 (26.4%)예였다. X-선 검사를 권유받은 1건 외에는 검사를 권유받지 않았다. 결론 : 이전 보고보다는 감기에서 항균제 사용률은 낮았으며, 진단을 하기 위한 검사가 적어 진단의 불분명을 극복하기 위한 것도 항균제 남용의 한 원인으로 생각된다. 주사제 처방률도 높은 듯하며 이는 빨리 증상이 호전되지 않을 때 병원을 바꾸는 환자들의 행위에 대한 반응일 가능성이 있으며, 항균제 사용 역시 빠른 증상 호전을 위한 것이어서, 환자의 행동 양상에 대한 조사가 필요하다.

파킨슨씨병 환자에 있어서 자가부신수질 이식술

정상섭,박상근,오성훈,김선호,윤도흠,박정수,양우익,최인준,안영수,김경환,박창일,김진수,이현철 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.6

Recent experimental studies and clinical cases have suggested that grafting tissue from the adrenal medulla into the brain may alleviate the symptoms of Parkinson's disease. Autologous adrenal medullary grafts into the striatum was performed stereotactically in two patients with Parkinson's disease. Both patients were bedridden preoperatively, but 4 months after the surgery both became ambulatory and one was even capable of returning to his job. Even though the number of cases was small⑵ and the follow-up period was short, this study shows that adrenal medullary transplantation into the striatum may have some beneficial effects in patient with Parkinson's disease. Further experimental and clinical trials are necessary to see whether this procedure will be a recommendable surgical treatment for patients suffering from Parkinson's disease.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

새로운 알랄아민 항진균제의 합성과 생물학적 평가

정병호,박은주,문현주,유진철 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

Some allylamine compounds which are benzothiazole substituants in stead of naphthylring in naftifine, antifungal agents, were sythesized as potential antimycotics. The intermediate Schiff bases that were obtained by condensation of 2-aminobenzothiazole and trans-cinnamaldehyde, were reduced to imine compounds to give allylamines(5a-5d) after mrthylation. These compounds which were tested in vitro against five fungal cell lines containing Trichophyton mentagrophytes. showed no activity in 0.1∼100 μg/ml range.

특수학급 학생의 성격특성과 식습관의 관계

정진자,김지현 한국특수교육문제연구소 2001 특수교육저널 : 이론과 실천 Vol.2 No.2

본 연구는 특수학급 학생의 성격특성과 식습관의 관계를 살펴보기 위해 서울, 인천, 전북지역에 소재한 중 ㆍ 고등학교 특수학급 학생 130명을 대상으로 식습관 조사를 위한 설문지와 어린이 및 청소년 성격유형 검사 (MMTIC)를 사용하여 얻어낸 결과 특수학급 학생의 성격 특성은 외향적,감각적,인식적,감정적인 성격을 갖고 있으며 특수학급 학생의 성격특성에 따라 식습관 형성인자,식사의 규칙성,간식의 종류,중점을 두는 식사,식사중 일어나지 않기 등에서 각각 다른 반응을 나타냈다. The purpose of this study was to investigate the relationship between the pcrsonality traits and food habit of students in special class. The subjects in this study were 130 selected special class students in middle and high schools in Seoul, lnchon, and Chollabukdo. The Following tests were given questionnaire of food habit and Murphy-Meisgeier Type lndicator for Children(MMTIC). The result of the present study were as following. First, personality traits of students in special class have extraversion, sensing, perceiving, feeling type. Second, the relationship between personality traits and the food habit of the special class students investigated, is as follows. The meal they found most delicious was lunch in those types of extraversion, intuition, thinking, judging but dinner in the perceiving type, lunch and dinner in sensìng type. Sensing type didn ’t have their meals regularly, the thinking type didn ’t show any distinct preference and the other types had them irregularly. When they got angry or was displeased, they didn't eat, but occasionally the intuition type ate. And all the types ate boiled rice and side dishes altemately. These four types of extraversion, sensing, thinking, judging left the table at meal but other four types of intraversion, intuition, feeling, perceiving rarely left the table at meal The side dishes were eaten evenly by all the types except the thinking type, but all the types left no boiled rice and had a meal three times a day. In eating speed these three types of extraversion, sensing, judging tended to eat with normal speed but other four types of intraversion, intuition, feeling, perceiving fast. The taste of food was the müst important element to these five types of extraversion, sensing, thinking, feeling, perceiving. But it was nutritive value to the intuition type and there was no special choice to the judging type. Recipe gave an average impact on their eating but the intuition type didn ’t eat the food with different cookery. All the types had breakfast everyday‘ The perceiving type didn ’ t eat breakfast because they had no appetite in the moming, but with the other types the reason was that they had no time. They ate between meals once a day, but the thinking type more than once or three times These two types of extraversion, judging ate between meals made by their own mothers and the other types bought to eat their favorite one. Their favorite food of eating between meals was soft drink or ice cream. These five types of extraversion, intuition, feeling, judging, percelVmg didn ’t had an unbalanced diet and other three types of intraversion, sensing, thinking didn ’t eat milk product. The thinking type had an unbalanced diet because of style and color, and other types because of taste. The most favorite food to the extraversion type was sweet one, pungent one and lightly seasoned one but to the intraversion type sourish one and to all the other types pungent one.

피압수가 존재하는 연약점토지반 내에 연직배수재의 관입깊이에 따라 압밀거동에 미치는 영향

정성교,진현식,백승훈 동아대학교 건설기술연구소 1999 硏究報告 Vol.22 No.2

In the Yangsan area developed for housing complex the soft clay deposited as about 20 to 40 meters in thickness and there was the Artesian pressure of about 20kPa under the clay stratum. To investigate the consolidation behaviour due to partially or fully penetrating vertical drains in the clay layer, a finite element analysis was carried out in this study. As a result of analysis, it was shown that the consolidation rate and the final settlement for the case of fully penetrating vertical drains were faster and less than of partially penetration, respectively.

Salmonella Typhimurium DT104

정현진,김의종 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.4

스포츠센터 소비자의 고객충성도에 영향을 미치는 요인에 관한 연구 : 서비스 품질과 서비스 가치를 중심으로 Centered on the quality and value of services

정의권,최영진,조현철 중앙대학교 스포츠과학연구소 2002 體育硏究 Vol.- No.17

This study centers on the quality and value of services perceived by consumers regarding factors that affect customer loyalties of sport center consumers. Study has been administered by a questionnaire survey of 200 male and female adult members of four sport centers in Seoul area. Following is the results: 1. Analyzing the relationship between demographic characteristics of sport center consumers and factors of their selection of those sport centers, there is significant difference in consumers' schooling and their family income. 2. Service quality perceived by sport center consumers appears to have a positive influence on service value. 3. Service value of sport center consumers appears to have a positive influence on customer loyalties. 4. Service quality perceived by sport center consumers appears to have a direct influence on customer loyalties. 5. Assessing the causal relationship between the service quality and value perceived by sport center consumers and customer loyalties, direct influence that the perceived service quality has on customer loyalties is stronger than indirect influence that mediated by service value.