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Hyo-Min Heo,Hyeong-WookJo,Kyu-Won Hwang,Jung-Hun Sun,Joon-Kwan Moon 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
Simultaneous analytical method using LC/MSMS for imidazolinone herbicides from livestock (egg, milk, beef, pork and chicken) for monitoring was developed with QuEChERS preparation. Sample weighing (5g) in a 50mL conical tube, added 0.1 M potassium phosphate dibasic solution (5mL) was shaken for 10min. After 0.5mL 6 NHCl, 10mL acetonitrile were added, it was shaken for 10min. And QuEChERS extraction salt (Original method, 4g MgSO₄, 1g NaCl) were added to the sample in the 50 mL conical tube. The mixture was strongly shaken for 1 min and was centrifuged at 3,000g for 10 min. The acetonitrile layer was purification with dSPE (150mg MgSO₄, 25mg C18) and was centrifuged at 13,000g for 5min. The supernatant was filtered with a membrane filters (pore size: 0.2um) before analysis. ME (%, Matrix effect) for almost analytes range were -6.56 to 5.70%. MLOD (Method LOD) and MLOQ (Method LOQ) was calculated by S/N ratio. MLOQs were 0.01 mg/kg. The linear correlation coefficients (r2) were > 0.99 within the range of 2 ~ 100 ug/kg for all of the 3 imidazolinone herbicides. The percentages (of imidazolinone herbicides) recovers were in the range of 84.1 ~ 110.6% (0.01mg/kg level), 90.8 ~ 97.1% (0.1mg/kg level) and 94.4 ~ 102.1% (0.5mg/kg level) within the validation criteria (recover; 70-120% with RSD < 20%)
Min Ji Kim,Jong Min Kim,Jong Hyun Moon,Hyo Lim Lee,Hye Rin Jeong,Min Ji Go,Tae Yoon Kim,Seung Gyum Joo,Ho Jin Heo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In this study, anti-inflammatory effect of the ethyl acetate fraction of Eucommia ulmoides leaves (EFEL) on particulate matter (PM)2.5-induced intestinal damage mice model was evaluated. The EFEL showed a cytoprotective effect against PM2.5-induced cell death in HT29 cells. The EFEL ameliorated reduced glutathione (GSH) and superoxide dismutase (SOD) level in intestinal tissue. In addition, EFEL inhibited the intestinal malondialdehyde (MDA) levels. Furthermore, myelin peroxidase (MPO) level using the marker of intestinal inflammation was decreased in the EFEL group. Furthermore, the inflammatory cytokines such as (IL-1β, TNF-α and p-IκB-α) levels were decreased in the EFEL group. Also, intake of EFEL protected the tight junction proteins such as occluding and claudin-1 on PM2.5-induced intestine damage mice.
Hyo Lim Lee,Jong Min Kim,Jong Hyun Moon,Min Ji Kim,Hye Rin Jeong,Min Ji Go,Tae Yoon Kim,Seung Gyum Joo,Ho Jin Heo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was conducted to assess the synbiotics interaction between the Corni fructus and Lactobacillus reuteri in a dextran sulfate sodium (DSS)-induced colitis and cognitive dysfunction in C57BL/6 mice. Mice were treated with Corni fructus (PRE; prebiotics), Lactobacillus reuteri (PRO; probiotics), and synbiotics (SYN; combination of Lactobacillus reuteri and Corni fructus) for 3 weeks. Consumption of PRE, PRO and SYN ameliorated body weight loss and colon-length shortening. Those treatments improved intestinal permeability by decreasing of in serum and increasing of tight junction protein expression in colonic tissue. Those also decreased the expression of inflammatory cytokine, and the myeloperoxidase activity. Especially, SYN significantly increased the content of short chain fatty acids in colitis mice fecal compared to PRE and PRO. Furthermore, PRE, PRO and SYN improved behavioral dysfunction by evaluating the Y-maze and passive avoidance tests. SYN significantly protected the antioxidant damages and cholinergic dysfunction, and regulated protein expression of acetylcholinesterase and choline acetyltransferase compared with PRE and PRO.
Min Ji Go,Jong Min Kim,Jong Hyun Moon,Min Ji Kim,Hyo Lim Lee,Hye Rin Jeong,Tae Yoon Kim,Seung Gyum Joo,Ho Jin Heo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In this study, the improvement effect of Korean red pine (Pinus densiflora) bark extract (PineXol<SUP>®</SUP>) against the behavioral dysfunction and neurotoxicity in amyloid beta (Aβ)1-42-induced ICR mice was confirmed. Consumption of PineXol<SUP>®</SUP> improved learning and memory dysfunctions compared to the Aβ group by evaluating the Y-maze, passive avoidance and Morris water maze tests. The PineXol<SUP>®</SUP> regulated acetylcholinesterase (AChE) activity, acetylcholine (ACh) content and expression of choline acetyltransferase (ChAT) and AChE related to a cholinergic system. In addition, the PineXol<SUP>®</SUP> restored antioxidant defisit by regulating the malondialdehyde (MDA) and reduced glutathione (GSH) levels in mouse brain tissue. Furthermore, PineXo<SUP>l®</SUP> significantly improved mitochondrial activity by ameliorating the mitochondrial membrane potential (MMP) and mitochondrial reactive oxygen species (ROS) level.
산수유의 Prebiotics 특성 및 Lipopolysaccharide로 유도된 장 상피세포 보호 효과
이효림(Hyo Lim Lee),이욱(Uk Lee),박선경(Seon Kyeong Park),강진용(Jin Yong Kang),김종민(Jong Min Kim),김길한(Gil Han Kim),김민지(Min Ji Kim),문종현(Jong Hyun Moon),정혜린(Hye Rin Jeong),박효원(Hyo Won Park),허호진(Ho Jin Heo) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.5
본 연구에서는 산수유(Corni fructus)의 유익균 증식에 대한 prebiotics로서의 활용 가능성과 LPS로 유도되는 세포독성에 대한 장 상피세포 보호 효과 및 주요 생리활성 물질을 분석하였다. 2% 산수유 물 추출물은 양성대조군인 2% fructo-oligosaccharide보다 L. reuteri 및 L. brevis의 적정산도 및 생육에 도움을 주었으며 특히 L. reuteri의 아세트산 생성을 증가시켰다. 그리고 산수유 물 추출물은 LPS로 유도된 장 상피세포(HT-29 cells) 독성에 대한 보호 효과와 산화적 스트레스를 감소시켜 주었으며, TLR4와 NF-κB의 발현을 효과적으로 저해하고 밀착연접 단백질의 발현은 증가시켜 주었다. HPLC 분석 결과 산수유 물 추출물의 생리활성물질은 morroniside와 loganin으로 확인되었다. 결과적으로 산수유는 유익균 증식과 LPS로 유도되는 세포 손상 및 장 질환 예방 소재로서의 활용 가치가 있을 것으로 판단된다. 하지만 산수유에 존재할 수 있는 미량의 페놀성 화합물동정 및 prebiotics 소재에 대한 평가로서 동물모델 상에서의 추가적인 연구가 필요할 것으로 판단된다. To evaluate the prebiotic effect of water extract from Corni fructus (WCF), we measured the growth curves, titratable acidity and short-chain fatty acid (SCFAs) contents in Lactobacillus reuteri (L. reuteri) and Lactobacillus brevis strains. After 24 h incubation, growth curve of the WCF-treated L. reuteri strain showed a relatively higher viable count and titratable acidity, as compared to the fructo-oligosaccharide (FOS)-treated strain (positive control). Considering that SCFA contents are useful metabolites for gut health, our results revealed higher contents of acetate in the WCF-treated strain (1,235.98±62.71 mM) than the FOS-treated strain (763.57±5.38 mM) of L. reuteri. DCF-DA [intracellular reactive oxygen species (ROS) content] and MTT assays [cell viability on lipopolysaccharide (LPS)-induced cytotoxicity] were applied in human colon epithelial HT-29 cells to determine the cell protective effect of the WCF. The results indicate that WCF effectively decreases the intracellular ROS contents and increases cell viability in LPS-induced HT-29 cells. To confirm the inhibitory effect of WCF on LPS-induced inflammation, we examined the expression levels of toll-like receptor 4 (TLR4) and nuclear factor-ĸB (NF-κB). Regulation of the LPS-induced inflammatory reaction by WCF was achieved by decreasing the expressions of TLR4 and NF-ĸB in HT-29 cells. Exposure to WCF also inhibited the cell permeability injury, as determined by increased expressions of tight junction proteins (occludin and claudin-1) in LPS-induced HT-29 cells, indicating the anti-inflammatory effect of WCF. Finally, morroniside and loganin, the major compound iridoid glycosides in the WCF, were analyzed by high-performance liquid chromatography. Taken together, our results indicate that Corni fructus is a potentially good natural source as a prebiotic to prevent intestinal dysfunction.
Regulatory Effect of Matcha Green Tea on Fine Dust-Exposed Cytotoxicity in Respiratory System
Jong Min Kim,Jong Hyun Moon,Min Ji Kim,Hyo Lim Lee,Hye Rin Jeong,Min Ji Go,Tae Yoon Kim,Seung Gyum Joo,Jong Cheol Kim,Ho Jin Heo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was performed to evaluate the protective effect of matcha green tea on particulate matter (PM)2.5-indued nasal and pulmonary cytotoxicity. The matcha green tea increased the cell viability and inhibited the ROS production in PM2.5-induced cytotoxicity in nasal RPMI2650 and pulmonary A549 cells. The matcha green tea regulated the expression of apoptosis, such as p-Akt, BCl-2, caspase-3, caspase-1 and HO-1, and inflammation, such as TLR4, TLR4 and Nrf2, in PM2.5-indued RPMI2650 cells. In addition, the matcha green tea showed regulatory effect of the apoptosis and inflammatory effect by increasing the BCl-2 and suppressing the caspase-3, TLR4, TNF-α and COX-2 in A549 cells. In addition, the matcha green tea suppressed antioxidant deficits by regulating the reduced GSH contents, SOD activities and MDA levels, and ameliorated the mitochondrial dysfunction by regulating the mitochondrial ROS production, mitochondrial membrane potential and ATP contents in pulmonary tissue. Ultimately, consumption of matcha green tea down-regulated the inflammatory proteins such as TNF-α, p-JNK, p-IκB-α, p-NF-κB and COX-2 in lung tissue.
Jong Min Kim,Jong Hyun Moon,Min Ji Kim,Hyo Lim Lee,Hye Rin Jeong,Min Ji Go,Tae Yoon Kim,Seung Gyum Joo,Jong Cheol Kim,Ho Jin Heo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was conducted to assess the protective effect of extract of matcha green tea (EM) against particulate matter (PM)2.5- and H₂O₂-induced dermal damage in primary dermal fibroblast and BALB/c mice. In the results of in vitro cell study, the EM increased the cell viability and decreased ROS production in PM2.5- and H₂O₂-induced primary dermal fibroblast cells by suppressing the inflammatory protein expression of caspse-3, caspase-1, p-JNK, p-NF-κB, COX-2 and IL-1β in primary dermal fibroblast cells. In addition, the EM protected the PM2.5-exposed antioxidant damage in dermal tissue by regulating the reduced GSH contents, SOD activity and MDA levels. The EM attenuated the inflammatory protein expression of TNF-α, TLR4, TLR2, p-JNK, BAX and COX-2 in dermal tissue.
Walnut Improves Particulate Matter (PM)<SUB>2.5</SUB>-Induced Neurotoxicity in HT22 Cells
Jong Hyun Moon,Jong Min Kim,Min Ji Kim,Hyo Lim Lee,Hye Rin Jeong,Min Ji Go,Hyo Won Park,Chul-Woo Kim,Uk Lee,Mahn-Jo Kim,Ho Jin Heo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was performed to evaluate the protective effect of ethyl acetate fraction from walnut (Gimcheon 1ho, GC) on particulate matter (PM)2.5-induced neurotoxicity in hippocampal HT-22 cells. The GC showed considerable total phenolics contents (TPC), total tannin contents (TTC) and total flavonoid contents (TFC). In addition, the GC presented significant 2,2’-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. Moreover, the GC presented significant antioxidant capacity by evaluating the ferric reducing/antioxidant power (FRAP) Furthermore, the GC showed the inhibitory effects of malondialdehyde (MDA) level and acetylcholinesterase (AChE) activity. In the result of PM2.5-induced neurotoxicity in HT-22 cells, the GC decreased ROS production and increased cell viability. Finally, the GC ameliorated protein expression levels of p-JNK, BCl-2, BAX and caspase-1 related to cell death in PM2.5-induced neurotoxicity.