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      • 중환자에서 동맥혈 가스분석 횟수와 그로인한 실혈량에 대한 연구

        이석열,전철우,정영수,오희숙,마분란,이정미,정혜영,이만복,이길노 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

        Background : Arterial blood gas analysis is the most comon exam in intensive care unit. In this study, the frequency of blood gas analysis and the amount of blood loss after Blood Gas Analysis in Intensive Care Unit were observed. Methods : From November 1999 to February 2000, 115 patients admitted to intensive care unit at Soonchunhyang university Kumi hospital were studied prospectively for the frequency of blood gas analysis and the amount of blood loss after blood gas analysis in Intensive Care Unit. Results : The mean value of frequency of blood gas analysis was 6.41±6.49 and the mean value of amount of blood loss after blood gas analysis was 0.78ml. This was lower than that of other foreign reports. The patients with ventilator therapy above 24 hours showed higher frequency of blood gas analysis than that of not applied ventilator patients. Pulse oxymetry was applied to all patients and the frequency of blood gas analysis was decreased due to the use of strict pulse oxymetry. Conclusion : Doctors and nurses in intensive care unit keep in mind that adequate blood sampling is needed for the decrease of blood loss. Education and protocol for the decrease of blood loss after arterial blood gas analysis may be necessary.

      • Helicobacter pylori와 대장균의 Shuttle Vector 개발

        조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

        In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

      • KCI등재

        Proper Tooth-Brushing Technique According to Patient’s Age and Oral Status

        Hye-Jeong Bok,Cheon-Hee Lee 대한예방치과학회 2020 International Journal of Clinical Preventive Denti Vol.16 No.4

        Tooth brushing is a basic technique to prevent major oral diseases (e.g., dental caries and periodontal disease). Several methods of tooth brushing technique devised by some dental scholars have been introduced. However, some guidelines to select the proper method are needed for an individual patient considering age and oral status. Methods such as rolling, Fones’, Bass, Stillman, Charters, Watanabe’s tooth-pick, and other modifications have been introduced. The rolling technique was recommended for general use, the Fones’ technique for preschool children, the Bass technique for the patient with gingivitis with gingival sulcus problem, the Stillman technique for widespread gingivitis, and the Charters technique for bridge wearer. Moreover, fixed-type orthodontic appliance wearer is recommended to use the mix-type methods as Bass, rolling, and Charters. Moreover, the Watanabe’s technique would be available for patients with a dental implant or periodontal problem. The denture wearer should clean the denture with a partial denture brush for a partial denture and a full denture brush for a full denture with the rolling method or its modification. The selection of the proper tooth brushing method suggested in this article would be one of the guidelines for clinical use of tooth brushing instruction for preventive measures.

      • KCI등재

        A Study on the Internal Oral Factors Affecting Components of Halitosis

        Hye-Jeong Bok,Eun-hee Lee 대한예방치과학회 2020 International Journal of Clinical Preventive Denti Vol.16 No.4

        Objective: The present study aimed to investigate the relationship between internal oral factors and components of halitosis and prepare clinically applicable data for efficient prevention of halitosis and establishment of a halitosis control plan. Methods: A hundred and one healthy adult men and women without systemic diseases were recruited in the present study. Values for each component of halitosis and internal oral factors, including oral hygiene index, using a disclosing agent and Quantitative light-induced fluorescence- device (AIOBIO, Seoul, Korea), salivary flow rate, oral humidity, and tongue plaque index, were measured, and a correlation analysis was performed for each figure. Results: According to the results of the association analysis between halitosis components and internal oral factors, hydrogen sulfide had a statistically significant relationship with simulated saliva and the tongue coating index (p<0.05). Methyl mercaptan was found to have a significant relationship with the O’Leary index and the simple plaque score (p<0.05). Conclusion: The above results suggest that of the internal oral factors studied, O’Leary index, simple plaque score, tongue coating index, stimulated saliva, etc., have associations with halitosis components

      • KCI등재

        Evaluation on Home Visiting Oral Health Program for the Elderly in Rural Community

        Hye-Jeong Bok,Wang-Keun Yoo,Cheon-Hee Lee 대한예방치과학회 2014 International Journal of Clinical Preventive Denti Vol.10 No.1

        Objective: The study was carried out to evaluate the performance of the home visiting oral health program of the elderly in rural community. One hundred twelve subjects, who were low income class, disabled and had chronic diseases, living in their own houses, institutions for the aged in two counties, Gyeongbuk Province were selected. And oral examination and face-to-face interview questionnaire survey for the subjects were implemented. Methods: The various indexes relating to oral health such as patient hygiene performance, calculus index, community periodontal index, tongue plague index, halitosis, saliva secretion, denture hygiene index were compared before and after the home visiting oral health program. The appraisal of the home visiting oral health program was done after 6 months of the program implementation. Results: The results showed that there was positive change in denture hygiene index, halitosis patient hygiene performance, tooth behavior such as brushing method, brushing times, while there was no positive change in calculus index, community periodontal treatment index, saliva secretion. Conclusion: Thus, various measures including provision of transportation for the visiting to medical facilities in order to improve the accessibility of the oral health services for the disabled elderly with low income, chronic diseases should be needed.

      • KCI등재

        Chorioamniotic membrane separation caused by the seromucinous collection from a placental chorioangioma

        ( Hye Mi Eom ),( Young Nam Kim ),( Byung Hee Choi ),( Eun Jeong Jeong ),( Jung Mi Byun ),( Dae Hoon Jeong ),( Moon Su Sung ),( Kyung Bok Lee ),( Ki Tae Kim ),( Hye Kyoung Yoon ) 대한산부인과학회 2016 Obstetrics & Gynecology Science Vol.59 No.3

        Placental chorioangioma is a benign non-trophoblastic tumor of the placenta that can have various adverse effects on the mother and fetus depending on its size. Chorioamniotic membrane separation is rare condition of detachment between the amniotic membrane and chorionic membrane. Chorioamniotic membrane separation after the second trimester of pregnancy is usually occurs after invasive procedures or may occur spontaneously; it is mostly associated with fetal abnormalities. Here, we report a case of chorioamniotic membrane separation that might be occurred caused by the seromucinous secretion from a placental chorioangioma.

      • KCI등재후보

        Engineering of Cell Derived-Nanovesicle as an Alternative to Exosome Therapy

        Jang Hye-Jeong,Shim Kyu-Sik,Lee Jinah,Park Joo Hyeon,Kang Seong-Jun,Shin Young Min,Lee Jung Bok,Baek Wooyeol,Yoon Jeong-Kee 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

        Background Exosomes, nano-sized vesicles ranging between 30 and 150 nm secreted by human cells, play a pivotal role in long-range intercellular communication and have attracted significant attention in the field of regenerative medicine. Nevertheless, their limited productivity and cost-effectiveness pose challenges for clinical applications. These issues have recently been addressed by cell-derived nanovesicles (CDNs), which are physically synthesized exosome-mimetic nanovesicles from parent cells, as a promising alternative to exosomes. CDNs exhibit structural, physical, and biological properties similar to exosomes, containing intracellular protein and genetic components encapsulated by the cell plasma membrane. These characteristics allow CDNs to be used as regenerative medicine and therapeutics on their own, or as a drug delivery system. Methods The paper reviews diverse methods for CDN synthesis, current analysis techniques, and presents engineering strategies to improve lesion targeting efficiency and/or therapeutic efficacy. Results CDNs, with their properties similar to those of exosomes, offer a cost-effective and highly productive alternative due to their non-living biomaterial nature, nano-size, and readiness for use, allowing them to overcome several limitations of conventional cell therapy methods. Conclusion Ongoing research and enhancement of CDNs engineering, along with comprehensive safety assessments and stability analysis, exhibit vast potential to advance regenerative medicine by enabling the development of efficient therapeutic interventions. Background Exosomes, nano-sized vesicles ranging between 30 and 150 nm secreted by human cells, play a pivotal role in long-range intercellular communication and have attracted significant attention in the field of regenerative medicine. Nevertheless, their limited productivity and cost-effectiveness pose challenges for clinical applications. These issues have recently been addressed by cell-derived nanovesicles (CDNs), which are physically synthesized exosome-mimetic nanovesicles from parent cells, as a promising alternative to exosomes. CDNs exhibit structural, physical, and biological properties similar to exosomes, containing intracellular protein and genetic components encapsulated by the cell plasma membrane. These characteristics allow CDNs to be used as regenerative medicine and therapeutics on their own, or as a drug delivery system. Methods The paper reviews diverse methods for CDN synthesis, current analysis techniques, and presents engineering strategies to improve lesion targeting efficiency and/or therapeutic efficacy. Results CDNs, with their properties similar to those of exosomes, offer a cost-effective and highly productive alternative due to their non-living biomaterial nature, nano-size, and readiness for use, allowing them to overcome several limitations of conventional cell therapy methods. Conclusion Ongoing research and enhancement of CDNs engineering, along with comprehensive safety assessments and stability analysis, exhibit vast potential to advance regenerative medicine by enabling the development of efficient therapeutic interventions.

      • KCI등재

        근로자의 직무스트레스가 구강건조감 및 구강증상에 미치는 영향

        조혜진 ( Hye Jin Cho ),조민희 ( Min Hee Cho ),조윤지 ( Yoon Ji Jo ),장영은 ( Young Eun Jang ),전은희 ( Eun Hee Jeon ),정복림 ( Bok Lim Jeong ),정윤선 ( Yoon Sun Jeong ),차혜경 ( Hye Kyung Cha ),최영주 ( Young Joo Choi ),한인성 ( I 한국치위생과학회 2012 치위생과학회지 Vol.12 No.6

        직무스트레스는 점차 복잡하고 다양화되어 가는 현대사회에서 건강 및 구강건강에 중요한 문제로 대두되고 있다. 본 연구는 2012년 6월 1일부터 6월 20일까지 366명의 근로자를 대상으로 직무스트레스와 구강건조정도를 파악하고 자가 구강증상을 측정하여 직무스트레스 및 구강건조정도와의 관련성을 알아보고자 하였다. 연구결과 다음과 같다. 1. 성별에 따른 직무스트레스 수준 차이 결과 남성은 직무요구, 관계갈등, 직장문화에서 상위 50%에 속해 있었으며, 여성은 관계갈등, 직무 불안정, 직장문화에서 상위 50%에 속해있었다. 2. 직무스트레스 수준에 따른 구강건조감과 자가 구강증상의 차이 결과, 구강건조감은 직장문화에서 유의한 차이를 나타냈다. 구강증상은 직무요구, 관계갈등, 조직체계, 보상부적절, 직무스트레스에서 유의한 차이를 나타냈다. 3. 구강건조감은 직장문화와 양의 상관관계를 나타냈으며, 구강증상은 직무요구, 관계갈등, 직무불안정, 조직체계, 직무스트레스, 구강건조감과 양의 상관관계를 나타냈다. 4. 직장문화, 보상부적절은 구강건조감에 유의한 영향을 주는 것으로 나타났으며, 구강건조감, 직무불안정, 조직체계는 구강증상에 유의한 영향을 주는 것으로 나타났다. 따라서 근로자 개인의 구강건강증진과 기업의 생산성 향상을 위해 직무스트레스의 원인을 정확히 파악하여야 하며, 스트레스로 인한 구강 내 증상을 인지하고 관리함으로써 삶의 질 향상에 큰 영향을 미칠 것이다. Job stress is emerging as one of major issues related to oral health in modern society that has increasingly been complicated and diversified. The purpose of this study was to examine the job stress and dry mouth of workers and their self-diagnosed oral symptoms in an attempt to determine the relationship between job stress and dry mouth. The subjects in this study were 366 workers, and a survey was conducted to June 1 to 20, 2012. Whether there would be any differences in dry mouth and self-diagnosed oral symptoms according to job stress was analyzed, and it`s found that there were significant differences in dry mouth according to organizational climate. And oral symptoms made significant differences to job demand, interpersonal conflicts, organizational system, lack of reward and job stress. There was a positive correlation between dry mouth and organizational climate, and oral symptoms had a significant correlation to job demand, interpersonal conflicts, job insecurity, organizational system, job stress and dry mouth. Organizational climate and lack of reward had a significant impact on dry mouth, and oral symptoms were under the significant influence of dry mouth, job insecurity and organizational system.

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