Glia-Like Cells from Human Mesenchymal Stem Cells Protect Neural Stem Cells in an In Vitro Model of Alzheimer's Disease by Reducing NLRP-3 Inflammasome

Hwang Mina,Song Se hyeon,장미숙,고성호 대한치매학회 2021 Dementia and Neurocognitive Disorders Vol.20 No.1

Background and Purpose: Neural stem cells (NSCs) have the ability to regenerate, proliferate, and differentiate, enabling them to play important roles in the recovery of the damaged nervous system. However, in neurodegenerative diseases such as Alzheimer's disease (AD), the NSCs are damaged as well. Glia-like cells from human mesenchymal stem cells (ghMSCs) are functionally enhanced adult stem cells. In the present study, we investigated whether ghMSCs could protect NSCs from amyloid beta (Aβ)-mediated toxicity. Methods: Rat NSCs were obtained from E13–14 fetal rat cortices. NSCs were seeded in pre-coated plates, and the next day, cells were simultaneously treated with 20 μM Aβ and 0.4 μm pore insert well-seeded ghMSCs. After 48 hours of co-treatment, cell viability and proliferation were evaluated. After 2 hours of co-treatment, western blotting was performed to measure inflammasome-related factors, such as NOD-like receptor family pyrin domain containing 3, caspase-1, and interleukin-1β. Results: The results showed that ghMSCs increased viability and proliferation and reduced the toxicity of NSCs injured by Aβ by reducing the NRLP3 inflammasome activation of NSCs induced by Aβ. Conclusions: In this study, we confirmed that ghMSCs could protect NSCs in an in vitro model of AD through the regulation of inflammatory response.

Effects of aspirin and clopidogrel on neural stem cells

Hwang, Mina,Park, Hyun-Hee,Choi, Hojin,Lee, Kyu-Yong,Lee, Young Joo,Koh, Seong-Ho Springer-Verlag 2018 Cell biology and toxicology Vol.34 No.3

Osteoblast-Like Cell Behaviors on Non-Woven Silica Fabric

Hwang, Mina,Yoon, Hanna,Lee, Yong Keun,Lim, Bum Soon,Rhee, Sang Hoon Trans Tech Publications, Ltd. 2006 Key Engineering Materials Vol.309 No.-

<P>The biological activity of osteoblast-like MC3T3-E1 cells on the newly developed non-woven silica fabric was investigated. The attachment, proliferation, and differentiation of osteoblast-like MC3T3-E1 cells were evaluated by MTS and alkaline phosphatase activity assays, respectively. The non-woven silica fabric showed higher biological activities than those of tissue culture plates with regard to attachment and proliferation while there was no significant difference with respect to differentiation. These results suggest that the non-woven silica fabric has a potential application as a bone grafting materia</P>

Relationship between social frailty, use of digital device, and depression among community-residing older adults

Mina Hwang,Yeji Hwang 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

3-5세 일반아동의 한글 인식 발달에 관한 연구

황지선(Hwang, Ji Sun),황민아(Hwang, Mina) 단국대학교 특수교육연구소 2008 특수교육논총 Vol.20 No.-

초등학교 고학년 읽기부진학생의 문장읽기에서 통사 처리 특성

황민아(Mina Hwang) 한국언어청각임상학회 2008 Communication Sciences and Disorders Vol.13 No.3

배경 및 목적: 읽기부진학생들의 통사처리 능력을 다룬 선행 연구들에 서는 주로 구어에 대한 문법검사를 이용하거나 메타통사적(metasyntactic) 과제에 대한 수행을 검 사함으로써 일상적으로 문장을 읽고 이해하는 과정에서의 통사처리 특성을 구체적으로 밝히지 못 하였다. 본 연구의 목적은 초등학교 고학년 읽기부진학생들이 문장을 읽고 이해할 때 통사처리의 특성을 조사하는 것이다. 방법: 초등학교 4~6학년 읽기부진학생 50명과 일반학생 52명을 대상으 로 구문 구조의 복잡성이 다른 두 유형의 문장(단순문장, 관형절 포함 복문)에 대한 문장-그림 확인 과제를 실시하였고, 이해정확도와 반응시간을 측정하였다. 단순문장은 규범어순(Subject-Object- Verb: SOV, 이하 SOV)과 비규범어순(Object-Subject-Verb: OSV, 이하 OSV) 문장을 포함하였 고, 관형절문장은 주격관형절과 목적격관형절을 포함하였다. 결과: 이해정확도의 측면에서 읽기부 진학생은 관형절문장 뿐 아니라 단순문장 이해에 있어서도 일반학생에 비하여 유의미하게 저조한 수행을 보였다. 두 집단 학생들 모두 단순문장에서는 OSV문장보다 SOV문장을, 관형절문장의 경 우에는 목적격관형절문장보다 주격관형절문장을 더 정확하게 이해하였다. 집단과 어순, 집단과 관 형절유형의 상호작용은 유의미하지 않았다. 이해반응시간에 대한 자료분석 결과, 단순문장이해 검 사에서 어순과 집단의 상호작용이 유의미하였는데, 일반학생들만 SOV문장에 대한 반응시간이 OSV문장에 비해 유의미하게 빨랐고, 읽기부진학생들에게는 어순에 따른 반응시간의 차이가 유의 미하지 않았다. 논의 및 결론: 읽기부진학생들은 전반적으로 일반학생에 비하여 통사처리에 어려움 을 보였지만 어순이나 관형절유형에 따른 이해검사의 수행 양상은 일반학생과 유사하였던 본 연구 의 결과를 통해 이들이 일반학생들과 비슷한 방식으로 통사분석을 한다는 것을 알 수 있었다. 그러나, 단순문장에 대한 이해반응시간에서의 집단과 어순의 상호작용은 읽기부진학생들이 일반학생들 에 비하여 문장이해시 어순정보를 활용하는 효율성이 떨어지는 증거로 여겨진다. Background & Objectives: Studies on syntactic skills of students with reading disabilities or poor readers focused on their metasyntactic skills or their subtest scores on grammar from standardized tests of spoken language, not on their syntactic processing during sentence reading. The purpose of the present study was to investigate the characteristics of syntactic processing of poor readers while they read and comprehended given sentences. Methods: A total of 102 elementary students (50 poor readers and 52 normal readers) at grades 4 to 6 participated in this study. Each participant performed two sentence comprehension tasks using two types of sentences of different syntactic complexity (simple sentences of noun-noun-verb form and complex sentences containing a relative-clause). A half of simple sentences were of SOV order and the other were of OSV order. Complex sentences contained either a subject relative-clause or an object relative-clause. A sentencepicture verification method was used for both sentence comprehension tasks and response times (RT) as well as response accuracies were measured. Results: The poor readers were significantly less accurate than the normal readers in performing both simple-sentence and complex-sentence comprehension tasks. Both groups of students’ comprehension accuracies were greater for SOV sentences than for OSV sentences, and were greater for subject relative-clauses than for object relative-clauses. No statistically significant interaction effects involving group and sentence types were noted in accuracy measures of the two tasks. In the analysis of RT for simple sentence comprehension, a significant interaction effect between group and word-order was noted. The normal readers were significantly faster in comprehending SOV sentences than in comprehending OSV sentences, while such RT difference across sentence types was not observed in the poor readers. Discussion & Conclusion: In general, the poor readers showed difficulties in syntactic processing during sentence comprehension relative to their normal peers. However, they did not exhibit atypical patterns of syntactic processing as their performance patterns in two sentence comprehension tasks were similar to those of the normal readers. The significant interaction effect between group and word-order of simple sentences in the RT measures was interpreted as an evidence of the poor readers’ inefficiency in utilizing word-order and case-markers in analyzing the syntactic structures of sentences.

단순언어장애아동의 문장 이해

황민아(Mina Hwang) 한국언어청각임상학회 2003 Communication Sciences and Disorders Vol.8 No.3

본 연구에서는 한국어를 사용하는 단순언어장애아동들이 세 가지 단서(조사, 어순, 생물성)를 체계적으로 조작한 문장을 이해할 때, 세 단서를 활용하는 양상과 그 정도를 언어연령이 일치된 정상아동들, 그리고 생활연령이 일치된 정상아동들과 비교하였다. 단순언어장애아동들이 세 단서 각각을 활용하는 양상은 정상아동들의 양상과 유사하였으나 그 정도에서는 차이가 있었다. 단순언어장애아동들은 두 정상아동집단보다 생물성단서에 의존하는 정도가 유의미하게 높았고, 조사단서를 활용하는 정도는 언어연령이 일치된 아동들과는 유사하나 생활연령이 일치된 아동들보다는 유의미하게 낮았다. 즉, 단순언어장애아동들이 조사단서를 처리하는 능력은 언어능력이 비슷한 정상아동과는 다르지 않지만, 상대적으로 의미적 단서인 생물성단서에 의존하는 경향이 크기 때문에 이들은 문장이해에서 의미적 단서의 영향을 정상아동들보다 더 많이 받는다고 볼 수 있다. The purpose of the present study was to investigate the sentence comprehension strategies used by Korean-speaking children with specific language impairment(SLI) within a framework of competition model. Fourteen children with SLI(SLI children)(ages 4;7- 7;2) and 14 age-matched normal children(CA children), and 14 younger children with matching language ages(LA children) participated in the study. In an actout procedure, the children were asked to determine the agents in sentences composed of two nouns and a verb with varying conditions of three cues(case-marker, animacy, and word-order). The results of group comparisons revealed that the SLI children relied animacy cues significantly more than the other two groups of normal children, and the two normal groups did not differ from each other in the use of animacy cues. In terms of case-marker cues, the CA children relied on case-marker cues significantly more than the other two groups, and the SLI children did not differe from the LA children. There were no significant differences between the groups in the use of word-order cues. The results indicated that although the SLI children use case-marker cues as much as the normal children with similar language skills, they tend to rely more on animacy cues when animacy cues are available.

문장따라말하기 검사의 학령 전 단순언어장애 진단 정확도

황민아(Mina Hwang) 한국언어청각임상학회 2012 Communication Sciences and Disorders Vol.17 No.1

배경 및 목적: 문장따라말하기 검사는 영어권 연구들을 포함한 다양한 언어권 연구들에서 단순언어장애 진단에 있어서 정확도가 가장 높은 검사로 보고되어 왔다. 본 연구에서는 문장따라말하기 검사가 학령 전 한국 아동의 단순언어장애 진단에도 유용한 검사인지를 확인하고자 하였다. 방법: 연구대상은 5~6세 단순언어장애 16명, 단순언어장애아동과 생활연령이 일치하는 일반아동 16명, 언어연령이 일치하는 일반아동 16명으로 총 48명이었다. 문장 길이와 구문 복잡도를 점진적으로 변화시켜 제작한 총 36 문항의 문장따라말하기 검사를 실시하였고, 아동의 수행은 문장단위, 낱말단위 두 가지 방법으로 채점하였다. 결과: 단순언어장애아동은 생활연령일치아동에 비하여 문장따라말하기 수행이 유의미하게 저조하였으나, 언어연령일치아동과는 유의미한 차이가 없었다. 아동들의 문장따라말하기 수행은 다른 언어검사 결과들 중 일부와 상관을 보였는데, 아동 집단마다 다소 다른 양상을 보였다. 문장따라말하기 검사 수행에 근거한 단순언어장애 진단의 민감도, 특이도 및 예측도는 5세, 6세 집단 각각에서 모두 85% 이상이었다. 5세, 6세 아동집단에서 양성우도비(positive likelihood ratio)는 각각 20.83, 11.0 이었고, 음성우도비(negative likelihood ratio)는 각각 0.13, 0.09 이었다. 논의 및 결론: 문장따라말하기 검사는 한국어를 사용하는 학령 전 아동들의 단순언어장애를 진단하는 검사로서 진단 정확도가 매우 높은 것으로 확인되었다. Background & Objectives: For children speaking various languages including English, Chinese and French, sentence repetition has been found to be one of the best clinical markers of specific language impairment. In the present study, the accuracy of a sentence repetition test in diagnosing specific language impairment among Korean preschool children was investigated. Methods: Sixteen five- to six-year-old children with specific language impairment, 16 typically developing children with matched chronological ages, and 16 younger children with matched language ages participated in the present study. A sentence repetition test containing 36 sentences of varying length and syntactic complexity was developed. The children's performances on the sentence repetition test were scored in two different ways. The sentence level scoring system followed the 'correct - incorrect' criteria used in most standardized tests where a single error made during sentence repetition lead to a 0 score. In the word-level scoring system, correctly repeated words were counted. Results: The children with specific language impairment performed significantly more poorly compared to their typically developing peers, but no significant difference was observed compared to the younger children with matching language skills. The children's performances on the sentence repetition test correlated significantly with performances on other language tests but in different ways across the groups. The sensitivity, specificity, and positive/negative predictive values of the sentence repetition test in diagnosing specific language impairment were greater than 85% in both the five- and six-year age groups. The positive likelihood ratios for these age groups were 20.83 and 11.0, and the negative likelihood ratios were 0.13 and 0.09, respectively. Discussion & Conclusion: The sentence repetition test was found to be very accurate in diagnosing specific language impairment among Korean preschool children aged five to six years.

5~7세 아동과 성인의 문법성판단 : 조사 오류를 중심으로

황민아(Mina Hwang),정미란(Miran Jeong) 한국언어청각임상학회 2007 Communication Sciences and Disorders Vol.12 No.2

Grammaticality judgment is widely used to reflect the development of metalinguistic awareness of children. In this study, grammaticality judgment skills of Korean- speaking children and adults were investigated. Methods: The participants were normally developing children ranging from 5-7 years old and college students. Their task was to judge the grammatical correctness of 48 short sentences, half of which contained incorrect case-markers and the other half were grammatically correct. In addition to the accuracy of responses, reaction times (RTs) of the participants’ decisions were measured. Four types of case-markers, including nominatives “i/ga”, accusatives “ul/lul”, a locative “e,” and an instrumental “ro,” were systematically changed to generate the grammatically incorrect sentences. Results: The participants’ sensitivity to grammatical errors was enhanced with age while their RTs decreased with age. They were most sensitive to errors of case-markers in nominative positions, followed by locative, instrumental and accusative positions. Errors in nominative positions had the shortest RTs while errors in accusative positions had the longest. Discussion & Conclusion: In terms of the patterns of response accuracy across the four types of erroneous case markers, the children 5-7 years old were similar to the adults. In terms of RTs, only the 7-year-old children were similar to the adults. The results’ implications are discussed on the basis of the acquisition of case-markers in Korean. 문법성판단은 메타언어 인식의 발달을 연구하는데 많이 이용된다. 본 연구에서는 메타언어인식 중 조사 오류를 탐지하는 능력의 발달을 조사하고자 하였다. 방법: 5~7세 일반아동과 성인을 포함한 140명을 대상으로 조사에 오류가 있는 문장에 대한 문법성판단과제를 실시하여 반응정확도와 반응시간을 측정하였다. 결과: 아동들은 연령이 증가할수록 A'으로 측정한 반응정확도가 증가하였고 반응시간은 감소하였다. 성별의 차이는 반응시간에서만 여자가 남자보다 유의미하게 더 빠른 것으로 나타났다. 조사에 오류가 있는 문장에 대한 오류 탐지 빈도와 반응 시간을 조사별로 분석한 결과, 오류탐지 빈도에서는 “-가”의 오류를 가장 잘 탐지하였고, “-에,” “-로,” “-을” 순으로 낮아졌다. 오류 탐지 반응시간의 경우 “-가”의 오류를 가장 빨리 탐지하였고, “-로”와 “-에”에 대한 반응시간은 서로 비슷하였으며 “-을”에 대한 반응시간이 가장 느렸다. 논의 및 결론: 조사별 오류 탐지 빈도에서는 5~7세 아동들 모두 성인과 유사한 반응 양상을 보였지만, 반응시간에서는 7세 아동의 반응양상만이 성인과 유사하였다. 본 연구의 결과를 아동의 조사습득 과정에 비추어 논의하였다.

도시내 방향별 섹터의 지가분포 특성에 대한 시계열적 분석

황민아(Hwang Mina),김정은(Kim Jeong Eun),윤기범(Yun Ki Bum),황희연(Hwang Hee Yun) 대한국토·도시계획학회 2012 國土計劃 Vol.47 No.4

The purpose of this study was to understand the change of land value distribution traits by distance from the center of Cheongju in alliance with street change by time series analysis. This study aimed to provide basic reference for future city plan and urban development policy. 4 sectors of Cheongju were set by development axis and preservation axis, and then basic data were analyzed to understand the spatial change. Then, land value distribution of each sector was analyzed to understand the form of land value distribution by distance from the center of the city. As the result of the analysis, despite the extension of the city caused by urban development, city center showed the highest land value. As the results, in each sector land value was the highest from the city center to l.5㎞ distance point. However, for further distance away from the city center, it was found that land value distribution was influenced by the city development such as housing developing site, etc.