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        Osteoblast-Like Cell Behaviors on Non-Woven Silica Fabric

        Hwang, Mina,Yoon, Hanna,Lee, Yong Keun,Lim, Bum Soon,Rhee, Sang Hoon Trans Tech Publications, Ltd. 2006 Key Engineering Materials Vol.309 No.-

        <P>The biological activity of osteoblast-like MC3T3-E1 cells on the newly developed non-woven silica fabric was investigated. The attachment, proliferation, and differentiation of osteoblast-like MC3T3-E1 cells were evaluated by MTS and alkaline phosphatase activity assays, respectively. The non-woven silica fabric showed higher biological activities than those of tissue culture plates with regard to attachment and proliferation while there was no significant difference with respect to differentiation. These results suggest that the non-woven silica fabric has a potential application as a bone grafting materia</P>

      • KCI등재

        Glia-Like Cells from Human Mesenchymal Stem Cells Protect Neural Stem Cells in an In Vitro Model of Alzheimer's Disease by Reducing NLRP-3 Inflammasome

        Hwang Mina,Song Se hyeon,장미숙,고성호 대한치매학회 2021 Dementia and Neurocognitive Disorders Vol.20 No.1

        Background and Purpose: Neural stem cells (NSCs) have the ability to regenerate, proliferate, and differentiate, enabling them to play important roles in the recovery of the damaged nervous system. However, in neurodegenerative diseases such as Alzheimer's disease (AD), the NSCs are damaged as well. Glia-like cells from human mesenchymal stem cells (ghMSCs) are functionally enhanced adult stem cells. In the present study, we investigated whether ghMSCs could protect NSCs from amyloid beta (Aβ)-mediated toxicity. Methods: Rat NSCs were obtained from E13–14 fetal rat cortices. NSCs were seeded in pre-coated plates, and the next day, cells were simultaneously treated with 20 μM Aβ and 0.4 μm pore insert well-seeded ghMSCs. After 48 hours of co-treatment, cell viability and proliferation were evaluated. After 2 hours of co-treatment, western blotting was performed to measure inflammasome-related factors, such as NOD-like receptor family pyrin domain containing 3, caspase-1, and interleukin-1β. Results: The results showed that ghMSCs increased viability and proliferation and reduced the toxicity of NSCs injured by Aβ by reducing the NRLP3 inflammasome activation of NSCs induced by Aβ. Conclusions: In this study, we confirmed that ghMSCs could protect NSCs in an in vitro model of AD through the regulation of inflammatory response.

      • KCI등재

        읽기부진 중학생의 다의동사 처리 특성

        황민아(Mina Hwang) 단국대학교 특수교육연구소 2020 특수교육논총 Vol.36 No.3

        연구목적: 읽기는 교과 과정을 학습해야 하는 학생들에게 반드시 요구되는 능력이다. 학년이 올라갈수록 글을 이해하기 위해서는 다양한 의미정보를 확인하고, 적절한 의미를 빠르게 찾아야 한다. 본 연구에서는 중학생 읽기부진학생들이 다의동사의 의미를 처리함에 있어 일반학생과 어떠한 차이를 보이는지 조사하였다. 연구방법: 본 연구는 중학교 1~3학년 읽기부진학생 11명과 일반학생 23명을 대상으로 하였다. 의미성판단과제를 통해 다의동사가 포함된 문장이 의미상으로 말이 되는지 여부를 판단하도록 하여 반응정확도와 반응시간을 측정하였다. 과제의 조건은 의미일치조건과 의미불일치조건으로 나뉘며, 점화 문장과 목표 문장에 포함된 다의동사의 의미의 일치여부에 따라 분류되었다. 연구결과: 읽기부진학생과 일반학생 모두 의미일치조건에 비해 의미불일치조건에서 반응정확도가 낮았으며, 반응시간이 더 소요되었다. 읽기부진학생의 경우 일반학생에 비해 두 조건 모두에서 반응정확도가 유의미하게 낮았으며, 특히 의미불일치조건에서 더 낮은 정확도를 보였다. 결론: 읽기부진학생은 일반학생에 비하여 목표문장의 의미적절성을 판단하는데 어려움을 보였다. 읽기부진학생은 불필요한 정보를 억제하는 데 어려움이 있는 것으로 보이며, 이것이 이들의 성공적 읽기 이해에 부정적인 영향을 미칠 수 있다. Purpose: Since the curriculum of secondary school includes advanced courses requiring extensive readings, middle school students with reading difficulties may experience more academic frustration. However, the studies on secondary students with reading difficulties in Korea were relatively limited. The purpose of the present study is to investigate the semantic processing of ambiguous verbs in middle school students with reading difficulties. Method: Of the students who participated in the study, 11 had reading difficulties while 23 did not. Two sentences containing a same ambiguous verb were presented consecutively on a computer screen. The participants were asked to judge whether each sentence made sense (Sensicality judgment task). In the consistent condition, the verb meaning of the first sentence(priming sentence) was identical to that of the following sentence(target sentence). Whereas, in the inconsistent condition, the verb meanings of the priming and the target sentences were different. Results: Compared to the normal peers, the students with reading difficulties were significantly less accurate in judging sensicality of the sentences containing ambiguous verbs. Both groups of students were significantly more accurate in the consistent condition than in the inconsistent condition. Significant interaction effect between group and condition was also noted. Both groups of students responded significantly faster in the consistent condition than in the inconsistent condition. No significant group difference and interaction effect were noted in response time to the target sentences. Conclusion: The students with reading difficulties performed poorly compared to the normal peers in determining the semantic appropriateness of a sentence containing an ambiguous verb, especially when a different meaning of the verb was used in the preceding priming sentence. They appeared to be ineffective in suppressing irrelevant word meanings activated previously. Unsuppressed meanings of the ambiguous verbs might interfere with the accurate interpretation of the sentences. Ineffective suppression of irrelevant information may negatively affect on reading comprehension of the students with reading difficulties.

      • KCI등재

        초등학교 고학년 읽기부진학생의 문장읽기에서 통사 처리 특성

        황민아(Mina Hwang) 한국언어청각임상학회 2008 Communication Sciences and Disorders Vol.13 No.3

        배경 및 목적: 읽기부진학생들의 통사처리 능력을 다룬 선행 연구들에 서는 주로 구어에 대한 문법검사를 이용하거나 메타통사적(metasyntactic) 과제에 대한 수행을 검 사함으로써 일상적으로 문장을 읽고 이해하는 과정에서의 통사처리 특성을 구체적으로 밝히지 못 하였다. 본 연구의 목적은 초등학교 고학년 읽기부진학생들이 문장을 읽고 이해할 때 통사처리의 특성을 조사하는 것이다. 방법: 초등학교 4~6학년 읽기부진학생 50명과 일반학생 52명을 대상으 로 구문 구조의 복잡성이 다른 두 유형의 문장(단순문장, 관형절 포함 복문)에 대한 문장-그림 확인 과제를 실시하였고, 이해정확도와 반응시간을 측정하였다. 단순문장은 규범어순(Subject-Object- Verb: SOV, 이하 SOV)과 비규범어순(Object-Subject-Verb: OSV, 이하 OSV) 문장을 포함하였 고, 관형절문장은 주격관형절과 목적격관형절을 포함하였다. 결과: 이해정확도의 측면에서 읽기부 진학생은 관형절문장 뿐 아니라 단순문장 이해에 있어서도 일반학생에 비하여 유의미하게 저조한 수행을 보였다. 두 집단 학생들 모두 단순문장에서는 OSV문장보다 SOV문장을, 관형절문장의 경 우에는 목적격관형절문장보다 주격관형절문장을 더 정확하게 이해하였다. 집단과 어순, 집단과 관 형절유형의 상호작용은 유의미하지 않았다. 이해반응시간에 대한 자료분석 결과, 단순문장이해 검 사에서 어순과 집단의 상호작용이 유의미하였는데, 일반학생들만 SOV문장에 대한 반응시간이 OSV문장에 비해 유의미하게 빨랐고, 읽기부진학생들에게는 어순에 따른 반응시간의 차이가 유의 미하지 않았다. 논의 및 결론: 읽기부진학생들은 전반적으로 일반학생에 비하여 통사처리에 어려움 을 보였지만 어순이나 관형절유형에 따른 이해검사의 수행 양상은 일반학생과 유사하였던 본 연구 의 결과를 통해 이들이 일반학생들과 비슷한 방식으로 통사분석을 한다는 것을 알 수 있었다. 그러나, 단순문장에 대한 이해반응시간에서의 집단과 어순의 상호작용은 읽기부진학생들이 일반학생들 에 비하여 문장이해시 어순정보를 활용하는 효율성이 떨어지는 증거로 여겨진다. Background & Objectives: Studies on syntactic skills of students with reading disabilities or poor readers focused on their metasyntactic skills or their subtest scores on grammar from standardized tests of spoken language, not on their syntactic processing during sentence reading. The purpose of the present study was to investigate the characteristics of syntactic processing of poor readers while they read and comprehended given sentences. Methods: A total of 102 elementary students (50 poor readers and 52 normal readers) at grades 4 to 6 participated in this study. Each participant performed two sentence comprehension tasks using two types of sentences of different syntactic complexity (simple sentences of noun-noun-verb form and complex sentences containing a relative-clause). A half of simple sentences were of SOV order and the other were of OSV order. Complex sentences contained either a subject relative-clause or an object relative-clause. A sentencepicture verification method was used for both sentence comprehension tasks and response times (RT) as well as response accuracies were measured. Results: The poor readers were significantly less accurate than the normal readers in performing both simple-sentence and complex-sentence comprehension tasks. Both groups of students’ comprehension accuracies were greater for SOV sentences than for OSV sentences, and were greater for subject relative-clauses than for object relative-clauses. No statistically significant interaction effects involving group and sentence types were noted in accuracy measures of the two tasks. In the analysis of RT for simple sentence comprehension, a significant interaction effect between group and word-order was noted. The normal readers were significantly faster in comprehending SOV sentences than in comprehending OSV sentences, while such RT difference across sentence types was not observed in the poor readers. Discussion & Conclusion: In general, the poor readers showed difficulties in syntactic processing during sentence comprehension relative to their normal peers. However, they did not exhibit atypical patterns of syntactic processing as their performance patterns in two sentence comprehension tasks were similar to those of the normal readers. The significant interaction effect between group and word-order of simple sentences in the RT measures was interpreted as an evidence of the poor readers’ inefficiency in utilizing word-order and case-markers in analyzing the syntactic structures of sentences.

      • KCI등재

        읽기이해부진아동의 어휘판단에서 단어길이 효과

        황민아(Mina Hwang),최경순(Kyung-Soon Choi) 한국언어청각임상학회 2011 Communication Sciences and Disorders Vol.16 No.4

        배경 및 목적: 읽기장애아동의 단어해호화 과정의 특성을 실시간(on-line)으로 측정한 연구들은 난독증 아동들에 집중되어 진행된 반면, 음독능력이 정상범주에 속하는 읽기이해부진아동들에 대한 연구는 드물었다. 자소-음소 전환이 규칙적인 언어들의 경우 단어 음독의 정확도가 아니라 속도가 읽기이해와 상관이 있다는 선행연구에 근거하면 읽기이해부진아동들의 음독 정확도가 일반아동들과 유사하더라도 단어를 읽고 재인하는 속도는 일반아동들에 비해 느릴 수 있다. 본 연구에서는 읽기이해부진아동들이 단어를 읽고 재인하는 과정의 실시간 특성을 밝히고자 하였다. 방법: 연구대상은 초등학교 4학년의 읽기이해부진아동 10명과 일반아동 12명이었다. 단어재인과정에서 단어 길이의 효과를 측정하기 위해 2음절과 4음절 단어에 대한 어휘판단 과제를 실시하였다. 결과: 읽기이해부진아동은 어휘판단의 반응 정확도와 반응속도 모두에서 일반아동에 비하여 유의미하게 저조한 수행을 보였다. 단어 길이 효과는 두 집단 아동들 모두에서 나타났지만, 읽기부진아동에게서 유의미하게 더 크게 나타났다. 논의 및 결론: 읽기이해부진아동들은 표준화된 읽기 검사에서 실시한 음독의 정확도가 일반아동들과 유사하였다. 그럼에도 불구하고, 본 연구의 어휘판단 과제에서 일반아동에 비하여 단어를 읽고 재인하는 정확도가 떨어지고, 속도가 느렸으며 이러한 경향은 긴 단어에서 더욱 강력하게 나타났다. 이러한 결과는 본 연구에 참가한 읽기이해부진아동들이 읽기이해 뿐 아니라 단어재인에서도 일반아동들에 비해 어려움을 겪는다는 것을 의미한다. 한글과 같이 자소-음소 전환이 규칙적인 언어에서 단어해호화 능력의 발달을 측정할 때 음독의 정확도만을 기준으로 삼는 것의 제한점과 이에 따른 읽기장애 분류의 문제점에 대하여 논의하였다. Background & Purpose: Although there have been many studies investigating the on-line word-decoding process during reading in dyslexic children using various languages, studies on word-decoding skills of children with poor reading comprehension are limited. Poor comprehenders are supposed to have normal word-decoding skills based on their accuracy scores in standardized word-reading tests. Reportedly, for children learning to read languages with transparent grapheme-phoneme correspondence, their reading comprehension is highly correlated with reading speed but not with the accuracy of word-reading. Since the graphemephoneme correspondence of the Korean language is known to be transparent, it is possible that Korean poor comprehenders are slower in recognizing words relative to typically developing children even though the accuracy of word-reading is similar in the two groups. Methods: Ten poor comprehenders and 12 typically developing children in 4th grade participated in the present study. The children performed a lexical decision task on words with two different lengths, two syllables or four syllables. Results: The poor comprehenders were significantly slower and less accurate in their performances on the lexical decision task. The word-length effect was significantly greater in the poor comprehenders, as the differences in speed and accuracy of lexical decision between short and long words were greater in the poor comprehenders compared to the typically developing children. Discussion & Conclusion: In a standardized reading test, the poor comprehenders only exhibited reading comprehension difficulties and were similar to the typically developing children in word-reading accuracy. However, the poor comprehenders' performances in the lexical decision task indicated they also had difficulties in word-recognition. The authors of the present study suggest that, in a language with transparent grapheme-phoneme correspondence, like the Korean language, using word-reading accuracy as a measure of word-decoding skills may not be appropriate. The importance of cross-linguistic differences of reading development in diagnosing various types of reading disabilities is also discussed in the present paper.

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