Streptozotocin유도 당뇨병이 백서하악 절단과두 치유에 미치는 영향의 실험적 연구

황의환,이상래,홍정표,김원철 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.2

The purpose of this study was to investigate the remodeling process of the streptozotocin-induced diabetic rat's resected condyle. This experiment was performed with male Sprague-Dawly strain rats weighing approximately 250gm, which were rendered diabetic by an intravenous injection of streptozotocin(70 ㎎/㎏ body weight). After condylectomy, experimental rats were serially terminated on the 1st week, the 2nd week, the 3rd week, and the 4th week. The following termination, the mandibles were dissected out to make specimens. Each mandibular condyle was radiographed with Hitex HA-80(Hitex Co., Ltd. Japan). In addition to radiographic observation, the mandibular condyles, further decalcified and embedded in paraffin, were sectioned and stained with Hematoxylin and Eosin, Toluidine blue and Masson's trichrome. The were observed with a light microscope and a polarizing microscope. The results were as follows. 1.Soft X-ray radiograms revealed proliferation of bone after 1 week in both groups. Irregularly repaired bones and dense trabeculae were clearly observed in experimental group. 2.The resected condyles were repaired by intramembraneous and endochondral bone formation in both groups. 3.Bone tissue repair was initiated from the adjacent margin of resected bone, and cartilginous tissues were observed at the top of repaired one in both groups. 4.The number of osteoblasts of experimental group was small, compared with control group. Each osteoblast was small and flap. The thin trabeculae were irregularly formed. 5.Collagens of bone were gradually matured in both groups, but the degree of maturation was lower in experimental group. 6.Fibrous tissues covered the upper parts of repaired bone were densely arranged in the both groups. Conclusively, atrophied osteoblasts, immature collagen of bone, and this and irregular trabeculae which were characterized in the diabetes experimental group showed diabetes disturbed osteoblastic function and caused disturbance of remodeling process of bone.

치성 및 비치성 조직이 악골 창상치유에 미치는 영향에 관한 실험적 연구

황의환,이상래,김소정,홍정표 大韓口腔顎顔面放射線學會 1996 Imaging Science in Dentistry Vol.26 No.2

This study was for comparing healing patterns and effects between with odontogenic and nonodontogenic tissues on the defected mandible. Experimental bone defects that measured 3 mm in diameter were created on the mandibular body of guinea pig by removal of bone with the use of trephine burs and bone defects were grafted with Biogran(Orthovita Co., U.S.A.)and covered with Dura Mata(Pfrimmer-Viggo GmbH Co., Germany). Guinea pigs were serially terminated by fours on the 3 day, the 1 week, the 2 weeks. the 3 weeks, the 4 weeks, and the 5 weeks after experiment, and the mandibular body was removed and fixed with 10% neutral formalin. They were decalcified adnembedded in paraffin as using the usual methods. The specimen sectioned and stained with hematoxylin and eosin and toluidine blue. They were observed with a light microscope and a polarizing microscope. The obtained results were as follows: 1. Defected bone was healed fast from the odontogenic tissues in early stage of the experiment. 2. The arrangement of the bone matrix was relatively regular in the bone from the nonodontogenic tissues, but irregular in the bone from the odontogenic tissues. 3. Compact bone has started to be resorbed and changed to the pattern of matrix bone tissue from 3 weeks after experiment.

불안저감치료음악을 통한 정신훈련이 골프퍼팅학습에 미치는 효과

황석연,박정래 公州大學校스포츠科學硏究所 1999 스포츠科學硏究所論文集 Vol.- No.13

The purpose of this study was to investigate the effect of mental practice accompanied by anxiety relaxation music therapy on golf putting Learning The subjects, forty male students in H Middle School, Who were willingly applied for the experiments, were divied into four groups (each group has 10 subjects) : the group for physical training, physical training accompanied by anxiety relaxation music therapy, mental-physical training, and mental training accompanied with music for anxiety relaxation therapy before physical training. The results of the experiments through 10 pre-tests, 60 acquisition phase, 20 retention phase were analyzed One-way ANOVA, which was the supplementary program of SPSS/PC+. In order to ascertain any differences among the groups another analysis was performed by Tukey's HSD. The results were the followings. 1) Mental Practice was effective to improve Golf Putting Learning. 2) Anxiety Relaxation Therapy Music was effective to improve Golf Putting Learning.

형질변형성장인자가 손상골 재생능에 미치는 영향에 관한 실험적 연구

황의환,이상래,홍정표,이병도 大韓口腔顎顔面 放射線學會 1995 Imaging Science in Dentistry Vol.25 No.2

The purpose of this study was to observe the effect of TGF-β1, which promotes differentiation and proliferation of osteoblasts, on bone regeneration. Experimental bone defects that measured 3 mm in diameter were created on the mandibles of guinea pig by removal of bone with the use of trephine burns. In one side of mandibular body the experimental groups, bone defects were grafted with Biogran(Orthovita Co., U.S.A.) and TGF-β1(R&D System Co., U.S.A.). IN the remaining side of the mandiblar body, the control groups, bone defects were grafted with only Biogran. Guinea pigs in the control and experimental groups were serially terminated by fours on the 3 days, the 1 week, the 2 weeks, the 3 weeks, and the 4 weeks after experiment, and both sides of the mandibular bodies were removed and fixed with 10% neutral formalin. They were decalcified and embedded in paraffin as using the usual method. The specimen sectioned and stained with hematoxylin and eosin. Also, they were radiographed with a soft X-ray apparatus The obtained results were as follows; 1. Hemorrhagic condition, observed in the granulation tissues, disappeared on the 1 week after experiment in both groups, and more prominent in the experimental group. The granulation tissues of the experimental group had larger number of cells than those of the control group. 2. Osteoblastic differentiation in the margin of grafted material and adjacent bone was observed on the 1 week after experimental in both groups. Also, bone formation was observed in immature form on the 1 week after experiment, and more prominent in the experimental group. 3. In the polarizing microscopic examination, bone matrix was very loose on the 1 week after experiment, but increase in density with time, and more prominent in the experimental group. 4. In the microradiographic examination, newly formed bone was observed in the experimental group on the 2 weeks after experiment, and this was observed earlier than in the control group. Newly formed bone was increased with time and defected area was markedly decreased on the 4 weeks after experiment.

원격 강의 활용 방안에 관한 연구 : 교내 네트워크 중심

황인선,김희수,박경우,조정래 광주보건대학 1998 論文集 Vol.23 No.-

We describe the distance learning at campus network. Many schools are now focusing on producing effective local network. Distance learning are using the PC, workstation, multimedia devices and be linked together. Distance learning has many distinguish characteristics: ① digital libraries. ② registration systems. ③ interation with legacy systems. And multimedia is material presented in a combination of text,graphics, video, animation and sound. A tool that gives the information product designer the freedom to link various kinds and pieces od data in a variety of ways so that students can moves through it campus network. A system capable of presenting multimedia material in its entirety.

Gas Chromatography를 이용한 Methoxime tert.-butyldimethylsilyl 유도체로서 α-Keto Acids의 분석에 관한 연구

金京禮,金正浩,金正漢,吳昌桓,黃恩卿 성균관대학교 약학연구소 1991 成均藥硏論文集 Vol.3 No.1

Abstract-Thirteen α-keto acids which are biochemically important were converted to methoxime(MO) derivatives with MOX and then to tert.-butyldimethylsilyl(TBDMS) derivatives using MTBS-TFA in isooctance prior to dual capillary column GC analysis. Single symmetric peaks were obtained for each MO TBDMS α-keto acids on the dual chromatograms. Optimum methoximation required heating at 60℃ for 2 hours and TBDMS derivatization required heating for 30 minutes. MO α-keto acids were stable during the tert.-butyldimethylsilylation and the methoximation of carbonyl group prevented silylation of enols. Mass spectrometric analysis of MO TBDMS derivatives was performed for structure identification. Base peaks at [M-57], intense ions at m/z=89, and weak ions at [M-15] were present in all spectra. The ions at m/z=89 were characteristic for α-keto acids.

HgCdTe 제작 다이오드 어레이의 열처리에 따른 전기적 특성

이정환,권순석,김명녕,이상래,황문구 大田産業大學校 2001 한밭대학교 논문집 Vol.18 No.-

This paper is investigated on the study of uniformity improvement and electrical characteristics for the 1×128 diode array used Hg^0.7Cd^0.3Te. The electrical characteristics were measured for the device manufactured by several different annealing processes and temperatures being discrepant from existing method of annealing processes and temperatures. The results of uniformity were improved from 60∼80[%], the rate of existing uniformity, to 99[%]. The results obtained from analyzing and examining the characteristics of current-voltage(I-V) and capacitance-voltage(C-V) are following. First, the reverse current show 6[㎁] or so. It is seemed to be resulted from the decrease of direct tunneling and trap tunneling of the device. Second, when the input bias voltage is zero, the maximum value of reverse resistance show 12[GΩ], and the mean value of reverse resistance show 5[GΩ]. Third, it is shown that the voltage of the maximum value of resistance is shifted 0.1[V] to the reverse direction.

형질변형성장인자가 손상골 재생능에 미치는 영향에 과한 실험적 연구

이병도,황의환,이상래,홍정표 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to observe the effect of TGF-81, which promotes differentiation and proliferation of osteoblasts, on bone regeneration. Experimental bone defects that measured 3 mm in diameter were created on the mandibles of guinea pig by removal of bone with the use of trephine burs. In one side of mandibular body, the experimental groups, bone defects were grafted with Biogran(Orthovita Co., U.S.A.) and TGF 131(R&D System Co., U.S.A.). In the remaining side of the mandiblar body, the control groups, bone defects were grafted with only Biogran. Guinea pigs in the control and experimental groups were serially terminated by fours on the 3 days, the 1 week, the 2 weeks, the 3 weeks, and the 4 weeks after experiment, and both sides of the mandibular bodies were removed and fixed with 10% neutral formalin. They were decalcified and embedded in paraffin as using the usual method. The specimen sectioned and stained with hematoxylin and eosin. Also, they were radiographed with a soft X-ray apparatus. The Obtained results.=were as follows; 1. Hemorrhagic condition, observed in the granulation tissues, disappeared on the 1 week after experiment in both groups, and more prominent in the experimental group. The granulation tissues of the experimental group had larger number of cells than those of the control group. 2. Osteoblastic differentiation in the margin of grafted material and adjacent bone was observed on the 1 week after experiment in both groups. Also, bone formation was observed in immature form on the 1 week after experiment, and more prominent in the experimental group. 3. In the polarizing microscopic examination, bone matrix was very loose on the 1 week after experiment, but increase in density with time, and more prominent in the experimental group. 4. In the microradiographic examination, newly formed bone was observed in the experimental group on the 2 weeks after experiment, and this was observed earlier than in the control group. Newly formed bone was increased with time and defected area was markedly decreased on the 4 weeks after experiment.

톨루엔 디이소시아네이트 폭로 근로자들의 작업방법에 따른 대사물질의 비교 및 면역능에 관한 연구

이수일,조병만,황인경,이철호,박정래 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.3

Following recent advanced industrialization, the amount of polyurethane to use as thermal insulating materials, upholstery, mattresses and packing materials in automotive and furniture industry is increasing world-widely, and the number of polyurethane-producing worker will be increased. Because the numerous organic solvents are used in polyurethane-producing factory, the workers in this work site is exposed to many organic solvents. Of the organic solvents, Toluene Diisocyanate(TDI) has many hazardous effects to human. The effects of TDI on human are the irritation to respiratory mucosa and gastrointestinal symptoms. Conjunctival irritation, dermal inflammation (redness, pain, vesicular formation) and gastrointestinal symptom(nausea, vomiting. abdominal pain) are reported just after short-term exposure of TDI. TDI is known to give rise to bronchial asthma, as the immune disorder. And because of strongly volatile characteristics of TDI, it is suggested as a more injurious material to human health, especially human immune system, than other organic solvents. Bronchial asthma inducing mechanism of TDI is not clearly known, but on the analogy of TDI induced symptoms and recent studies, early-onset asthma is type I hypersensitivity reaction mediated by immunoglobulin E(IgE), and late-onset asthma is maybe type III hypersensitivity reaction by circulating IgG. And we know that the complicated human immune function is likely to move in such that mechanisms, there are not studies on immune indices evaluating the bronchial asthma-related immune function. The evaluation of change patterns of humoral immunity including IgE and IgG and cellular immunity including T-helper cell. T-suppressor cell and T-cytotoxic cell will be helpful to evaluate exposure degrees and prognosis in TDI exposed workers. Because TDA(toluene diamine) as a biological exposure index of TDI becomes the focus of interest, we know that a study on the correlation between urinary TDA and air TDI and immunological indices will make a contribution to biological effect monitoring indicies. We examined human immunity indicators such as WBC, %Lymph (percentile of Lymphocyte in WBC), %T-cell(percentile of T-lymphocyte in total lymphocyte). CD4, CD8, C3, C4, IgA, IgG, IgM, IgE in peripheral blood to evaluate the health hazard of the TDI-exposed workers. And we examined TDA to evaluate correlation between exposure and effect. Total 90 subjects was selected, 45 workers who worked in the polyurethane-producing factories as an exposed group, and 45 cases who were office workers(10 cases), other blue collors(27 cases), and medical college students(8 cases) as a control group. And the results were as follows; 1. The logarithm of IgE-Log10(IgE)±SD-in peripheral blood of a exposed group was significantly higher than a control group, 2.22 ±0.62 in case group compared with 1.98±0.53 in control group. (p<0.05) 2. IgA and IgM in the polyurethane-producing workers were 261.02±83.12㎎/㎗, 151.97 ±59.64 ㎎/㎗, respectively, and 292.77±100.45, 179.17±100.78 in control group. IgA and IgM was slightly lower in polyurethane-producing group than control.(p>0.05) 3. WBC, %Lymph. %T-cell, C3, C4, CD4, CD8, CD4/CD8 ratio and IgG in case group were 6.391.1 ea/㎖, 37.53 %, 59.54 %, 76.68 ㎎/㎗, 0.76×10(9) ea/L, 0.63×10(9) ea/L, 1.39, and 1606.29 ㎎/㎗, respectively, and 6,974.7 ea/㎖, 35.12 %, 59.64 %, 71.95 ㎎/㎗, 33.94 ㎎/㎗, 0.80×10(9) ea/L, 0.61×10(9) ea/L, 1.39, and 1581.51 ㎎/㎗ in control group. There was no statistical significance between two groups.(p>0.05) 4. In the comparison of each other companies, average of individual urinary TDA in polyurethane paint manufacturing companies is higher than that of polyurethane sponge foaming companies. And, the concentration of 2.6-TDA which is a metabolite of well-vaporized 2.6-TDI is higher than that of 2.4-TDA in the polyurethane sponge foaming companies. But, the concentration of 2.4-TDA which is a metabolite of illvaporized but well skin-absorbed 2.4-TDI is higher in polyurethane paint manufactures. 5. There were no statistical significance in the correlations between individual urinary TDA and immunologic indices.

실내공간 기반 IndoorGML 데이터 편집 및 가시화 구현에 관한 연구

황정래 ( Jung-rae Hwang ),최진원 ( Jin-won Choi ),최현상 ( Hyun-sang Choi ),홍창희 ( Chang-hee Hong ),이지영 한국정보처리학회 2012 한국정보처리학회 학술대회논문집 Vol.19 No.2

최근 시설물이 대형화 및 복잡화 되어 가면서 실외 공간뿐만 아니라 실내공간에 대한 관심이 국내외적으로 높아지고 있다. 이에 실내공간을 3차원으로 표현하고, 실외와 실내를 연계 및 통합하려는 연구가 국내외적으로 시도되고 있다. 본 연구에서는 실외와 실내의 연계 및 통합을 위한 전 단계의 연구로 현재 국제 표준화 작업이 진행 중인 실내공간을 표현하는 IndoorGML 데이터모델을 대상으로 그 데이터를 편집하고 가시화하는 도구를 구현하였다.