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Han, Mi Na,Byeon, Hyeon Seop,Han, Seong Tae,Jang, Rae Hoon,Kim, Chang Seop,Choi, Seok Hwa The Korean Society of Veterinary Service 2018 韓國家畜衛生學會誌 Vol.41 No.4
Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.
Han, Mi Na,Byeon, Hyeon Seop,Lee, Cho Yeon,Jo, Nam Sin,Lee, Jong Hwa,Jang, Rae Hoon,Kim, Chang Seop,Na, Ki Jeong The Korean Society of Veterinary Service 2019 韓國家畜衛生學會誌 Vol.42 No.4
Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the 'A' farm and 3~72 weeks old in the 'B' farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm 'A' and 'B' were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was 'GKQGRLIG' which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by entire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid sequence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.
Han, Mi-Na,Kim, Jeong-Ho,Lee, Sang-Kab,No, Kyong-Ok,Chae, Mun-Hui,Cho, Woo-Kyoung,Lee, Jong-Hwa,Jang, Rae-Hoon,Kim, Chang-Seop,Byeon, Hyeon-Seop The Korean Society of Veterinary Service 2020 韓國家畜衛生學會誌 Vol.43 No.2
This case describes outbreaks of acute aspergillosis in a red-crowned crane. A six-month-old, male, crane had showed clinical signs (i.e. anorexia, performance loss, ruffled feathers and drooped wings and open mouth breathing, etc.) before death. In necropsy examination, spherical to oval nodules disseminated from the respiratory tract to other organs. Those nodules were formed predominantly in air sacs, lung, peritoneum, serosa of esophagus and trachea. The nodules varied in size from 1 mm to over 1cm and the color was white to yellow. Microscopically, most of lung architecture were replaced by multiple foci which were characterized by well demarcated eosinophilic and karyorrhetic debris and surrounded by numerous Inflammatory cell. Most within necrotic center of the nodules, large numbers of fungal hyphae were present. Microbiology result indicated fungal growths on sabroud dextrose agar and bacterial growths on blood agar. Bacteria identified as E. rhusiopathiae using MALDI-TOF (microflex, BRUKER, USA) and fungi identified as A. fumigatus, A. terreus by sequencing the ITS1 and ITS4 regions. To confirm the route of infection, we checked the existence of the same pathogens in cohabitant (i.e. mother crane). The young age and weakened immunity (i.e. bacterial infection, etc.) causes fatal aspergillosis in birds.
생화학적 마커와 Microsatellite 마커를 이용한 KCR 랫드의 유전모니터링
박한진(Han-Jin Park),이철호(Chul-Ho Lee),차달선(Dal-Seon Cha),조재우(Jae-Woo Cho),조규혁(Kyu-Hyuk Cho),최양규(Yang-Kyu Choi),현병화(Byung-Hwa Hyun),조진원(Jin-Won Cho),한상섭(Sang-Seop Han),송창우(Chang-Woo Song) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.2
The cataract mutant rat which showed lens opacity was found in the colony of Sprague-Dawley rat at Korea Research Institute of Chemical Technology (KRICT) in 1996. The mode of inheritance of affected rats was autosomal recessive. These cataractous rats were mated by sister and brother to 27th (F₂₇) generation and named as Korea Cataract Rat (KCR). We performed genetic monitoring of KCR rat using biochemical and microsatellite markers to determine their genetic identified homozygous and distinctive genetic profile. In biochemical monitoring, six rats from each of the F₁₈/F₁₉F₂₀ were tested using 13 biochemical markers. In molecular genetic monitoring, nine rats from the F₂₅ and F₂₆ were tested using 13 microsatellite markers. No differences were found among the genetic profiles of the F₁₈/F₁₉F₂₀ in biochemical monitoring. Also, in the molecular genetic monitoring, we identified that both genetic profiles of F₂₅ and F₂₆ were the same. The genetic profile of KCR rats was unique against the other major rat strains. In addition, the polymorphysm of KCR rat for microsatellite markers was different from the other strains. These results suggest that KCR rat is genetically well qualified as far as in this test and it has been well established as a new inbred strain which has a novel genetic profile and cataract trait.
인하대병원에서의 17,744 건의 자궁경부 세포진 검사 결과
김종화,한상훈,박지현,임문환,송은섭,임영구,이병익,한지영,오관영 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.3
목적: 저자들은 인하대학교 의과대학 부속병원(인하대병원) 산부인과에서 실시한 자궁경부 세포진 검사의 결과를 분석하고 조직검사와 비교하여 차후에 중요한 자료로 이용하고자 하였다. 연구 방법: 1996년 5월 인하대병원이 개원한 이래 1998년 8월까지 28개월 동안 산부인과 외래에서 시행한 17,744건의 자궁경부 세포진 검사의 결과와 그 조직학적 검사결과를 연구내용으로 하였다. 결과: 정상과 양성 세포 변화가 93.2%, ASCUS가 2.9%, LSIL이 0.9%, HSIL이 1.4%, SCC가 0.8% 나왔고, ASCUS/SIL 비율은 1.28 이었다. 조직검사에서는 ASCUS의 경우 악성으로 나온 경우가 4.0%, LSIL에서 15.7% AGUS의 경우 9.3%로 낮게 나온 것에 비하여 HSIL에서는 48.0%, SCC에서는 56.1%, Adenoca에서는 69.2%로 높게 나왔다. 결론: 정상과 양성 세포 변화가 93.2% , ASCUS가 2.9%, LSIL이 0.9%, HSIL이 1.4%, SCC가 0.8% 나왔고, ASCUS/SIL 비율은 1.28 로 나와 기존의 발표와 매우 유사함을 보였으며 세포진의 결과가 나쁠수록 조직검사의 결과에서도 나쁜 것을 나왔다. Objective: Our purpose was to investigate the distribution of the result of 17,744 cervicovaginal smears and their pathologic results in Inha University Hospital to make a reference data. Methods: During 28 months, May 1996 to August 1998, we performed 17,774 cervicovaginal smears and arranged them by the Bethesda system and compared them with their pathologic results. Results: Among 17,744 smears, there were 93.2% of benign results, 2.9% of ASCUS, 0.9% of LSIL, 1.4% of HSIL, and 0.8% of SCC. The ASCUS/SIL ratio was 1.28. Among ASCUS, the high grade histologic outcome was composed 4.0%. Among LSIL, there were 15.7%, and HSIL 48.0%, SCC 56.1%, AGUS 9.3%, and Adenocarcinoma, the results was 69.2%. Conclusion: Among 17,744 smears, there were 93.2% of benign results, 2.9% of ASCUS, 0.9% of LSIL, 1.4% of HSIL, and 0.8% of SCC. The ASCUS/SIL ratio was 1.28. These results were very similar to other data and we would like to add our data to them as a reference. And the worse the smear results were, the worse the pathologic results were.
Mi Na Han,Hyeon Seop Byeon,Cho Yeon Lee,Nam Sin Jo,Jong Hwa Lee,Rae Hoon Jang,Chang Seop Kim,Ki Jeong Na 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.4
Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the ‘A’ farm and 3∼72 weeks old in the ‘B’ farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm ‘A’ and ‘B’ were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was ‘GKQGRLIG’ which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by en-tire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid se-quence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.
Mi-Na Han,Jeong-Ho Kim,Sang-Kab Lee,Kyong-Ok No,Mun-Hui Chae,Woo-Kyoung Cho,Jong-Hwa Lee,Rae-Hoon Jang,Chang-Seop Kim,Hyeon-Seop Byeon 한국동물위생학회 2020 韓國家畜衛生學會誌 Vol.43 No.2
This case describes outbreaks of acute aspergillosis in a red-crowned crane. A six-month-old, male, crane had showed clinical signs (i.e. anorexia, performance loss, ruffled feathers and drooped wings and open mouth breathing, etc.) before death. In necropsy examination, spherical to oval nodules dissemi-nated from the respiratory tract to other organs. Those nodules were formed predominantly in air sacs, lung, peritoneum, serosa of esophagus and trachea. The nodules varied in size from 1 mm to over 1cm and the color was white to yellow. Microscopically, most of lung architecture were replaced by multiple foci which were characterized by well demarcated eosinophilic and karyorrhetic debris and surrounded by numerous Inflammatory cell. Most within necrotic center of the nodules, large numbers of fungal hyphae were present. Microbiology result indicated fungal growths on sabroud dextrose agar and bacte-rial growths on blood agar. Bacteria identified as E. rhusiopathiae using MALDI-TOF (microflex, BRUKER, USA) and fungi identified as A. fumigatus, A. terreus by sequencing the ITS1 and ITS4 regions. To con-firm the route of infection, we checked the existence of the same pathogens in cohabitant (i.e. mother crane). The young age and weakened immunity (i.e. bacterial infection, etc.) causes fatal aspergillosis in birds.