인체 췌장암세포주(Capan-1)의 증식에 미치는 amiloride의 억제효과

임대관,김신,김유리,노지훈,이지현,김지연,박무인,정근옥,박건영,구자영 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

Background/Aims Cytoplasmic alkalinization induced by activation of the Na+/H+anti porter which is stimulated upon the addition of growth-promoting agents, such as insulin, epidermal growth factor, phorbol ester, plays an essential role in the initiation on cell proliferation. In the present study the effects of amiloride, a specific and reversible inhibitor of Na+/H+antiporter, on the growth of human pancreatic carcinoma cell line, Capan-1 cells was examined and the effects of 5-fluorouracil (5-FU) were also studied. Cell cycle analysis was done to examine the mechanisms for the inhibitory effects of amiloride. Materials/Methods The growth of Capan-1 cells were examined by counting cell number on two and four days treatment with 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 80 μM, 160 μM amiloride, and 0.1 ㎍/㎖, 0.3 ㎍/㎖ 5-FU, after plating Capan-1 cells into 35-mm2 plastic dishes at d density of 10x104 cells/dish. The reversibility of the effects of amiloride was examined on two day to eight days treatment with 20 μM amiloride after seeding 2×104 cells/dish. Cell cycle analysis was done on the sells after four days treatment with 20 μM amiloride. Results Amiloride significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion (p<0.05). The inhibitary effect of amiloride on the growth of Capan -1 cells was firstly shown at the concentration of 5 μM, which is not so higher than the concentration of 0.1-0.2 μM attainable by administration of usual dose of amiloride (5-10㎎). Forty-eight percent inhibition of growth was found at an amiloride concentration of 20μM after 4 days treatment, and ninety-three percent inhibition of growth was found at an amiloride concentration of 160μM after 4 days treatment. The inhibitory effect of amiloride on growth of Capan-1 cells was reversible since removal of amiloride by a media change after 48 hours treatment lead to significantly more growth than amiloride treated group (p<0.05). The reversibility of growth inhibition suggests that amiloride in not a non-specific cytotoxin for Capan-1 cells. Amiloride combined with 5-FU significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion compared to an amiloride or a 5-FU alone (p<0.05). After four days treatment with 20 μM amiloride, the faction of cells in G0-G1 phase, S phase and G2-M phase was 47.3%, 35.8%, 16.9% respectively in the amiloride group (20 μM), and 44.3%, 37.1%, 18.6% in the control group. showing no significant differences between the two groups. Conclusions Amiloride significantly inhibited the growth of Capan-1 cells in a dose-dependent fashion, which was reversible. The reversibility of growth inhibition suggests that amiloride is not a non-specific cytotoxin for Capan-1 cells. The concentration of 5 μM, which is not so higher than the concentration of 0.1-0.2 μM attainable by administration of usual dose of amiloride (5-10㎎), which suggests amiloride or its analogues may be used alone or in conjunction with 5-FU for the treatment of pancreatic carcinoma. Further study is needed to clarify the effects of more potent analogues of amiloride on the growth of human pancreatic carcinoma cells.

골관절염 환자에서 관절내 Sodium Hyaluronate(Hyruan®)의 투여가 슬관절통에 미치는 영향

김훈정,임경준,위상우,정종달,유벙식,안태훈,소금영 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background : Sodium Hyaluronate has been found to be deficient in the synovial fluid of patients with osteoarthritis. We evaluate the effect of intraarticular Sodium Hyaluronate(Hyruan®) on the knee joint pain in patients with osteoarthritis. Method : Ten patients with osteoarthritis of the knee were participated in this study. Sodium Hyaluronate was injected into knee joints weekly for 5 weeks consecutively. Clinical evaluations were performed using visual analogue scale(VAS) for pain, tenderness and swollen joint counts weekly for the first 5 weeks and at 10 weeks postinjection. Result : VAS, tenderness and swollen joint were all significantly improved from 2 weeks to 10 weeks. There was no complication, except pain on injection site in two patients. Conclusion : Intraarticular injection of Sodium Hyaluronate has been shown to be effective on the treatment of patients with osteoarthritis of the knee.

척수강내 Bupivavacain에 첨가한 소량의 Neostigmin의 진통효과와 부작용

소금영,정종달,김병철,유벙식,안태훈,김훈정,임경준 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background : Spinal cholinergic receptor has been shown to have a antinociceptive action, an effect that can be mimicked by spinal cholinesterase inhibitor. Intrathecal injection of neostigmine cause analgesia and adverse effect in a dose-dependent pattern in the patients. The purpose of this study was to determine whether small doses of intrathecal neostigmine (10, 25 and 50 ㎍) produce analgesia and any side effects. Method : After getting informed consents, 60 patients scheduled for orthopedic surgery of lower extremities under spinal anesthesia were randomly assigned to one of four groups. Using dose-ranging design, patients received either normal saline or neostigmine l0, 25 or 50 ㎍ in a 1-㎎ solution of 5% glucose in normal saline with heavy bupivacaine 12 ㎎. Heart rate, blood pressure, degree of motor and sensory block were recorded. The assessment of postoperative analgesia included time to first rescue, total number of rescue medication and pain score on visual analog scale (VAS) at 1, 3, 6, 9, 12, 24 hour. Nausea and vomiting were assessed by using the visual analog scale at 24 hr postperatively and the incidence of them was recorded postoperatively. Result : There were no significant differences among the four groups in characteristics of spinal blocks. Compared to saline, neostigmine groups significantly prolonged time to first rescue medication and decreased total number of rescue medication and pain visual analog scale score, but incidence of nausea and 24hr nausea VAS score were not increased in neostigmine group compared to control group. Conclusion : These data in patients injected intrathecal neostigmine suggest that analgesia may occur at doses less than neostigmine 50 ㎍ and neostigmine 50㎍ has a better analgesia effect with fewer side effects than other doses for postoperative analgesia.

[PD-0036] CAM Biodesign: Engineering Crassulacean Acid Metabolism (CAM) Photosynthesis into C₃ plants for Improving Water-use Efficiency

Sung Don Lim(Sung Don Lim),Hong-Ju Lee(Hong-Ju Lee),Yu Kyong Hu(Yu Kyong Hu),Jeong Ho Shin(Jeong Ho Shin),Sang Hun Kim(Sang Hun Kim),John C. Cushman(John C. Cushman ) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

관절강내 투여된 Tramadol 과 Neostigmine 이 슬관절경 수술 후 통증완화에 미치는 영향

김승수,오경희,임경준,유병식,소금영,김훈정,정종달,안태훈 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.41 No.4

The Effects on Pain Relief of Intraarticular Tramadol and Neostigmine after Knee Arthroscopic Surgery Kyung Joon LIM, M.D., Hun Jeong Kim, M.D., Kyung Hee Oh, M.D. Tae Hun An, M.D., Keum Young So, M.D., Byung Sik Yu, M.D. Chong Dal Chung, M.D., and Seung Soo Kim, M.D.^* Department of anesthesiology, College of Medicine, Chosun University, Gwangju, Korea, and ^*Seoul Pain Clinic, Gwangju, Korea Background: Evidence has accumulated that tramadol hydrochloride can produce relief of moderate to severe pain across the range of acute and chronic pain states by combining a synergistically weak opioid and a monoaminergically mediated antinociceptive mechanism. Neostigmine can produce anti-nociceptive effects by interacting with muscarine receptors in peripheral tissues. This study was designed to determine whether intraarticular tramadol results in better analgesic effect and whether tramadol and neostigmine would provide superior analgesia to tramadol alone, after knee arthroscopic surgery. Methods: Forty-five ASA class 1 or 2 patients undergoing arthroscopic knee surgery were randomly allocated to three treatment groups. All patients received general anesthesia with nitrous oxide, O_2 and inhalational agents. When the surgical procedure was completed, the study drug was injected into the patient's knee joint through the arthroscope. Patients in group 1 (n = 15) received 30 ml of 0.5% mepivacaine; patients in group 2 (n = 15) received tramadol 50 mg and 30 ml of 0.5% mepivacaine; patients in group 3 (n = 15) received a combination of tramadol 50 mg, neostigmine 100 ㎍ and 30 ml of 0.5% mepivacaine. Postoperative pain was assessed using the visual analogue scale (VAS) at 1, 2, 4, 6, 12 and 24 hours after the intraarticular injection. Results: There were no significant differences among the three groups in the 1 to 2 hour postoperative period and groups 2 and 3 showed singificantly lower VAS score than group 1 from 4 to 24 hours postoperatively. Conclusions: It is concluded that after knee arthroscopy, intraarticular injection of tramadol had a good analgesic effect, whereas neostigmine added to tramadol did not show superior analgesic effects over tramadol alone. (Korean J Anesthesiol 2001; 41: 461~466)

The Relationship Between Ankle Muscles and An EMG-Based Physically Interactive Game

( Yu-min Ko ),( Seol Park ),( Ho-cheol Lee ),( Chang-hun Lim ),( Ji-won Park ) 대한물리치료학회 2015 대한물리치료학회지 Vol.27 No.6

Purpose: This study was to identify the relationship between the game score and muscle strength in order to elucidate whether the obtained score for the dorsiflexor and plantar flexor muscles in the ankle joint using an EMG-based interactive game system can reflect muscle strength as measured conventionally. Methods: Forty adults were enrolled in the present study. They had no congenital deformities, and no neurological or orthopedic disorders in the 6 months prior to the start of the study. The Biodex were used to measure the isokinetic concentric maximal strength of the plantar flexor and dorsiflexor muscles in the ankle joint. EMG electrodes were attached to the tibialis anterior and gastrocnemius. Results: (1) There was a positive relationship between the obtained game score by the plantar flexor (sPF) and muscle strength of the plantar flexor (tPF) and dorsiflexor (tDF). In addition, the tPF affected the sPF, but the tDF did not. Thus, the higher the tPF, the higher the sPF. (2) There was no relationship between the obtained game score of dorsiflexor (sDF) and tPF or tDF. In addition, neither the tDF or tPF affected the sDF. Conclusion: The game score had a relationship with muscle strength, which is related to ankle instability and re-impairment. Thus we suggest that this game system can be used to predict the degree of weakness of muscle strength.

Prediction of post-stroke dementia using NINDS-CSN 5-minute neuropsychology protocol in acute stroke

Lim, Jae-Sung,Oh, Mi Sun,Lee, Ju-Hun,Jung, San,Kim, Chulho,Jang, Min Uk,Lee, Sang-Hwa,Kim, Yeo Jin,Kim, Yerim,Park, Jaeseol,Kang, Yeonwook,Yu, Kyung-Ho,Lee, Byung-Chul Cambridge University Press 2017 INTERNATIONAL PSYCHOGERIATRICS - Vol.29 No.5

<B>ABSTRACT</B><B>Background:</B><P>The National Institute of Neurological Disease and Stroke-Canadian Stroke Network (NINDS-CSN) 5-minute neuropsychology protocol consists of only verbal tasks, and is proposed as a brief screening method for vascular cognitive impairment. We evaluated its feasibility within two weeks after stroke and ability to predict the development of post-stroke dementia (PSD) at 3 months after stroke.</P><B>Method:</B><P>We prospectively enrolled subjects with ischemic stroke within seven days of symptom onset who were consecutively admitted to 12 university hospitals. Neuropsychological assessments using the NINDS-CSN 5-minute and 60-minute neuropsychology protocols were administered within two weeks and at 3 months after stroke onset, respectively. PSD was diagnosed with reference to the American Heart Association/American Stroke Association statement, requiring deficits in at least two cognitive domains.</P><B>Results:</B><P>Of 620 patients, 512 (82.6%) were feasible for the NINDS-CSN 5-minute protocol within two weeks after stroke. The incidence of PSD was 16.2% in 308 subjects who had completed follow-up at 3 months after stroke onset. The total score of the NINDS-CSN 5-minute protocol differed significantly between those with and without PSD (4.0 ± 2.7, 7.4 ± 2.7, respectively; p < 0.01). A cut-off value of 6/7 showed reasonable discriminative power (sensitivity 0.82, specificity 0.67, AUC 0.74). The NINDS-CSN 5-minute protocol score was a significant predictor for PSD (adjusted odds ratio 6.32, 95% CI 2.65-15.05).</P><B>Discussion:</B><P>The NINDS-CSN 5-minute protocol is feasible to evaluate cognitive functions in patients with acute ischemic stroke. It might be a useful screening method for early identification of high-risk groups for PSD.</P>

Korean Association for Clinical Oncology : Slide Session ; P-35 : Head & Neck Cancer ; Early-Stage Oral Cavity Cancer: Can We Expect a Curative Role of Defl nitive Radiotherapy?

( Yu Jin Lim ),( Hong Gyun Wu ),( J Hun Hah ),( Myung Whun Sung ),( Kwang Hyun Kim ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Both radiotherapy (RT) and primary surgery are treatment options in early-stage oral cavity squamous cell carcinoma (OC-SCC). This study evaluated the impact of local treatment modalities and pathologic features on patients` survival in cT1-2N0 OC-SCC. Methods: We reviewed the 167 patients with biopsy-proven cT1-T2N0 OC-SCC patients who underwent defi nitive radiotherapy (RT) (n = 33) or surgery ± postoperative RT (PORT) (n = 134) from 1990 to 2012. Cervical lymph node dissection (LND) was performed in 75 (56%) patients, and 25 (19%) patients underwent PORT. The median radiation dose for defi nitive RT and PORT were 70 Gy (range, 50.4-71.4) and 65.4 Gy (range, 50.4-67.5), respectively. Results: The median follow-up duration was 45.3 months (range, 4.4-262.4). The 5-year rates of cause-specifi c survival (CSS) in defi nitive RT and surgery were 53.8% and 85.5% (P < 0.001), respectively. The major treatment failure after defi nitive RT was local recurrence (n = 11 in 33). Defi nitive RT resulted in worse 5-year local control rate than that of surgery (29.1% vs. 84.7%, P < 0.001), and the causes of defi nitive RT (with or without medical fragility) did not impact on local control (P = 0.235). In the patients treated with surgery alone without occult lymph node metastasis (n = 106), multivariate analyses identifi ed moderately/poorly-differentiated grade and lymphatic invasion (LI) as prognostic predictors in CSS and relapse-free survival (RFS). The presence of both higher tumor grade and LI showed worse CSS and RFS compared to other patients without any of the risk factors (P < 0.001 for both). Conclusions: Defi nitive RT in cT1-2N0 OC-SCC obtained a lower cure rate compared to primary surgery group. The subgroup analyses of the low-risk patients with surgical resection alone identifi ed a high-risk group who may benefi t from PORT.

Carbon hybrid fillers composed of carbon nanotubes directly grown on graphene nanoplatelets for effective thermal conductivity in epoxy composites

Yu, Lan,Park, Ji Sun,Lim, Yun-Soo,Lee, Churl Seung,Shin, Kwonwoo,Moon, Ho Jun,Yang, Cheol-Min,Lee, Young Sil,Han, Jong Hun IOP Pub 2013 Nanotechnology Vol.24 No.15

<P>Carbon nanomaterials are generally used to promote the thermal conductivity of polymer composites. However, individual graphene nanoplatelets (GNPs) or carbon nanotubes (CNTs) limit the realization of the desirable thermal conductivity of the composite in both through- and in-plane directions. In this work, we present the thermal conductivity enhancement of the epoxy composite with carbon hybrid fillers composed of CNTs directly grown on the GNP support. The composite with 20 wt% hybrid filler loading showed 300% and 50% through-plane thermal conductivity improvements in comparison with the individual CNTs and GNPs, respectively. Moreover, it showed an enhanced thermal conductivity of up to 12% higher than that of the simply mixed GNP and CNT fillers. In more detail, hybrid fillers, whose CNTs were synthesized on the GNP support (Support C, Fe/Mo–MgO:GNP=1:0.456) for 60 min via chemical vapor deposition process, presented the highest through-plane thermal conductivity of 2.41 W m<SUP>−1</SUP> K<SUP>−1</SUP> in an epoxy composite.</P>

Mechanism and Motion of New Biped Leg Machine

Hun-ok Lim,Yu Ogura,Atsuo Takanishi 제어로봇시스템학회 2005 제어로봇시스템학회 국제학술대회 논문집 Vol.2005 No.6