Transformation of Pisum sativum L. var sparkle; A Non Tissue Culture Method

CHOI, Hong Jib,PARK, Soon Ki,YOON, Young Hwi,KIM, Dal Ung 慶北大學校農業科學技術硏究所 1993 慶北大農學誌 Vol.11 No.-

완두에 있어서 보다 효율적인 형질전환 방법을 모색하고 형질전환된 개체를 얻고자 본실험을 수행하여 얻어진 결과는 다음과 같다. 형질전환은 발아중인 완두의 생장점(shoot tip)을 제거한 다음 T-DNA내에 GUS gene과 neomycin phosphotransferase Ⅱ gene이 들어있는 binary vector를 가진 Agrobacterium tumefaciens를 생장점을 제거한 부위에 감염시켰다. 감염후 새로 형성된 shoot는 개체당 4∼5개 였으며, 그중 GUS유전자가 발현하는 shoot만을 정상적인 식물체로 분화 시켰다. 감염부위에서 형성된 shoot에서의 GUS유전자의 발현빈도는 10%내외였다. 이들 개체로 부터 genomic DNA를 분리하여 Dot blot hybridization분석 결과 T-DNA가 식물체 내에 존재함을 알수 있었고, 수확한 종자를 발아시켜 Sorthern blot hybridization한 결과 T-DNA가 다음세대로 전달되었음이 확인되었으며 형질전환율은 2%이내였다. The transfer of genetic material into pea tissue was accomplished by using an avirulent strain of Agrobacterium tumefaciens containing the binary vector. The method used for transformation requires non-tissue culture steps as it involves the inoculation of the site of the shoot removed of germinating seeds. The identification of β-glucuronidase activity in the tissues of ?? pea plants indicates that the plant expressible β-glucuronidase gene, contained the T-DNA region from pLPBO2, had been transferred at least into somatic tissues. Putative transformed ?? pea plants were advanced to produce T₁plants which were also assayed for the presence of the transferred β-glucuronidase gene. The presence of the β-glucuronidase gene in DNAs isolated from T₁plant was demonstrated by DNA gel blot hybridization. This analysis revealed that the transformed plants contained β-glucuronidase gene.

Effect of Seeding Time on Fatty Acid Composition, Oil Contents and Seeds Yield in Flax

Choi, Hong Jib,Park, Shin Young,Kim, Sang Kuk The Plant Resources Society of Korea 2012 한국자원식물학회지 Vol.25 No.6

Influences of different seeding dates on growth, seed yield, fatty acid composition and oil content were investigated in flax plants for two years. The results indicated that plant height in early seeding date was higher than that of delayed seeding dates during first season. Furthermore, seeding date also significantly affected the ripened seed rate and the rate increased with the delay in seeding date in first season. Seed yield in the first crop season was significantly higher than the second crop season. Palmitic acid showed variation in different seeding dates. Contrarily, stearic acid was stable and did not changed by different seeding dates. Linolenic acid was found in highest amount in all seeding dates consecutively in two cropping years. Highest oil content was recovered from the seeds of flax sown at 29 Apr. and May 9 in first and second cropping year respectively.

Reciprocal differences in low temperature germination and seedling vigor in several inbred lines of sweet corn (Zea mays)

Choi Hong-Jib,Huh Chang-Shuk,Sohn Chang-Ki 한국작물학회 2006 한국작물학회 학술발표대회 논문집 Vol.2006 No.-

참외 과실의 무산소처리가 발효관련 대사물질 발생에 미치는 영향

최성국(Choi Sung Kuk),김동헌(Kim Dong Hern),최홍집(Choi Hong Jib),최부술(Choi Boo Sul) 한국원예학회 1999 원예과학기술지 Vol.17 No.2

진행성 비소세포 폐암에 대한 5 - Fluorouracil , Vinblastine 과 Cisplatin ( FVP ) 의 복합화학요법

최홍집(Hong Jib Choi),김성록(Sung Rok Kim),양성현(Sung Hyun Yang),최수전(Soo Jeon Choi),김철수(Chul Soo Kim),김예희(Re Hwe Kim) 대한내과학회 1997 대한내과학회지 Vol.53 No.1

The 5 year survival rate after surgical resection for stage III non-small cell lung cancer(NSCLC) remains short, only 10-19%. Radiotherapy seems to show some benefit for local control, but it does not reflect to prolongation of survival, as distant metastasis prevails. Vinblastine is one of the most active agent against NSCLC. 5-Fluorouracil(5-FU) and cisplatin are synergistic in anti-cancer activity in adenocarcinoma of gastrointestinal tract and squamous cell cancer of head and neck. We initiated a phase II trial for advanced NSCLC to determine the effect of FVP regimen in response rate, survival and toxicities. The therapy consisted of 5-FU 500mg/m2/12 hours continious IV infusion for 36 hours from day 1, vinblastine 3mg/m2 IV bolus day 1 and 2, cisplatin 75mg/m2 IV infusion over 2 hours day 1 and it was repeated every 3 weeks. Among the 45 patients entered into this study, 40 patients were evaluable for response. The objective response rate was 50%(CR;1/40, 2.5% PRi19/40, 47.5%). The median survival of all the patients was 42.9 weeks(8.4+ - 140.6 weeks); the responding patients survived longer than the non-responders(mediansurvival; 54.4 weeks vs 29.7 weeks, p<0.05). The toxicities of this regimen were acceptable but 1 patient died of pneumonia associated with granulocytopenia. We concluded that the FVP regimen is effective in the treatment of advanced non-small cell lung cancer and a prospective randomized trial and long- term follow up is warranted.

블렌딩 오일 시장 확대를 위한 소비자 선호 분석

최돈우 ( Don Woo Choi ),최홍집 ( Hong Jib Choi ),홍나경 ( Na Kyoung Hong ) 한국식품유통학회 2012 食品流通硏究 Vol.29 No.2

The number of obese people is on the rise in Korea because of westernized eating habits. Accordingly, consumers` concern about cooking oil also increases as trans fats are known to be the cause of obesity. The purpose of this paper is to investigate consumers` preference for the blending oil on the grounds that there`s ideal blending oil which mix omega 3·6·9 fatty acids in the ratio of 2:1:1. A choice experiment is designed and the aroma and prices of sesame oil, flaxseed oil and blending oil are examined as attributes. 154 Daegu metropolitan consumers were surveyed and a mixed logit model was employed to estimate WTPs. The result shows the highest WTP on the blending oil, the second highest on the flaxseed oil, the third on the sesame oil and the last on the aroma. Consumers have positive attitudes on health functional food or functionality-enhanced blending oil. The result of this paper could contribute to the development of functionality-enhanced blending oil production through transferring agricultural technology and improvement of rural household incomes.

Evaluation of forage yield and quality for wild soybean accessions and progenies derived from a cross Glycine soja × G. max

Eun-Ja Lee,Hong-Jib Choi,Dong-Hyun Shin,Chan-Ho Kwon,J. Grover Shannon,Jeong-Dong Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Soybean is desirable as a forage crop because of it has high protein and oil concentration. Wild soybean, a progenitor of cultivated soybean, has a softer stem and higher protein content in seed than cultivated soybean. There is little information on yield and forage quality for wild soybean and its derivatives. The objective of this study was to determine the forage yield and quality of wild soybeans and selected soybeans derived from a cross G. max ×G. soja. Forage yield and quality were assessed for three grain soybean cultivars, three wild soybeans and three selected lines from G. max×G. soja. Forage quality attributes such as crude protein (CP), crude fat (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), digestible dry matter (DDM), dry matter intake (DMI) and relative feed value (RFV) were determined at the R2, R4 and R6 developmental stages. Forage yield and CF were highest at stage R6 in G. max, G. soja and selected G. max×G. soja lines. CP content was similar between R2 and R4 but increased sharply after R4 and peaked at R6 in G. max and selected lines from G. soja×G. max. On the other hand, CP content was similar between R4 and R6 stage in wild soybeans. Generally, NDF and ADF were highest at stage R4 but decreased at stage R6. DDM, DMI, and RFV increased between R4 and R6. These results suggest that R6 was the optimal harvest stage to provide forage of highest quality and yield. A study was conducted in 2011 to evaluate forage yield and quality at stage R6 in 25 lines from PI483463 (G. soja)×Hutcheson (G. max) and four cultivated grain soybeans. Hutcheson had the highest forage yield with 24.7t/ha infresh weight (FW) among grain soybeans. Line W11 had the highest forage yield(25.7t/ha,FW) among G. soja×G. max selections and four other lines had similar forage yield compared to Hutcheson. Generally the 25 lines from this G. max×G. soja cross had thinner main stems and branches than cultivated soybeans. When the 25 lines were evaluated for their feed quality as per forage grade by AFGC, nine lines rated prime grade and all 25 lines were classified as forage Grade 1. Results of this study indicate crosses between wild and cultivated soybean show promise for improving soybean as a forage crop.

Genetic diversity of super-sweet corn inbred lines using SSR and SSAP markers

Woo Ri Ko,Hong-Jib Choi,Kyu Jin Sa,Ju Kyong Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

In this study we evaluate the informative and efficiency of Simple Sequence Repeat (SSR) and Sequence Specific Amplified Polymorphism (SSAP) markers for genetic diversity, genetic relationship and population structure among 87 super sweet corn inbred lines generated by different origins. The SSR showed relatively higher level of the average gene diversity and shannon’s information index value than that of the SSAP. To assess genetic relationship and to characterize among 87 super sweet corn inbred lines using the SSR and SSAP markers. The dendrogram using SSR marker divided into nine groups of clusters were observed at the genetic similarity value 53.0%. For SSAP marker, Total three main clusters were confirmed in genetic similarity value at 50.8%. Result of combine data for SSR and SSAP markers showed six subgroup were detected in genetic similarity at 53.5%. To confirm population structure, the total 87 super sweet corn inbred lines were divided into groups I, II and admixed group based on membership probability 0.8 for SSR and SSAP markers. However population structure using combine data was K=3 and divided into group I, II, III and admixed group. This study has demonstrated the comparative analysis of SSR and SSAP for the study of genetic diversity and the genetic relationship for super sweet corn inbred lines. Thus, the results of this study will be useful to maize breeding programs in Korea.

Perilla屬 植物이 형태적,세포유전학적 특성 및 지방산 조성 분석

최홍집,권일찬,김재현,김달웅 慶北大學校農業科學技術硏究所 1993 慶北大農學誌 Vol.11 No.-

本 硏究는 들깨 국내 육성종을 포함한 蒐集種중 20種을 공시하여 이들 간의 形態的인 主要 特性, 生化學的인 特性과 細胞遺傳學的인 特性을 調査하고 遺傳的 多樣性을 밝혀 들깨의 分類와 品種有成에 있어서의 重要한 基礎 資科를 얻고자 本 實驗을 遂行하여 얻은 結果를 要約하면 다음과 같다. 들깨 수집종의 形態的, 生化學的, 細胞遺傳學的 特性을 調査해 본 바, 形態的 特性으로 種皮色은 약간의 농도차이는 있었으나 灰褐色, 褐色, 暗褐色(흑색)등 3群으로 나눌 수 있었으며, 于粒重은 3.5g 이하인 것이 11種으로 가장 많았고 4.5g 이하인 것이 4種 4.5g 이상인 것이 5種이었다. 또한 줄기색은 赤色과 綠色으로 분류되었는데 赤色은 안동재래를 포함 5種이었고 나머지는 綠色이었다. 生化學的인 特性으로 각 蒐集種을 대상으로 脂肪酸 조성을 調査해 본 바, 불포화도가 가장 높은 linolenic acid는 53%(영양재래)에서 65%(의성재래)에 이르기 까지 多樣하였으며, 59-61% 범위에 속하는 種이 12種으로 가장 많았다. 染色體 數는 조사한 4개의 蒐集種(안동재래, 엽실, 영광재래, 대영) 모두가 2n=38로 나타났다. This study was conducted to obtain the basic information for the classification of P erilla spp. based on their major morphological characteristics for the 20 local collections of perilla distributed in Korea. The results of this experiment were summarized as follows : Several morphological characteristics were investigated. Seed color was classified to four groups with greshywhite, greshybrown, brown and darkbrown. The 1,000 grain weight, 11 accessions were below 3.5g, four accessions was 3.6g to 4.5g and five accessions was 4.5g to 5.07g. There was two types of stem color, red and green, 15 accessions was green and 5 accessions including Andongjaerae, Yeupsil, Ockdong, Cheongsongjaerae and Chugjaso were red color. In fatty acid composition was 60% in average. Among 20 accessions, Euseongjaerae showed the highest content(65%) and Yeongyangjaerae the lowest(54%) linolenic acid content. In the cytogenetical studies on the four accessions including Andong, Yeupsil, Yeongkwang and Daeyeong, the number of chromosomes were 2n=38.

Proteomic analysis of differential protein expression in response to epidermal growth factor in neonatal porcine pancreatic cell monolayers

Hong, Oak-Kee,Suh, Sun-Hee,Kwon, Hyuk-Sang,Ko, Seung-Hyun,Choi, Yoon-Hee,Moon, Sung-Dae,Yoo, Soon-Jib,Son, Ho-Young,Park, Kyung-Soo,Lee, In-Kyu,Yoon, Kun-Ho Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of cellular biochemistry Vol.95 No.4

<P>We have proposed that porcine neonatal pancreatic cell clusters (NPCCs) may be a useful alternative source of cells for islet transplantation, and that monolayer cultures might provide an opportunity to manipulate the cells before transplantation. In addition we previously identified 10 genes up-regulated by epidermal growth factor (EGF) in cultured porcine NPCC monolayers. We have now analyzed the intracellular signaling pathways activated by EGF and searched for proteins differentially expressed following EGF treatment of the monolayers, using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). EGF treatment resulted in phosphorylation of both Erk 1/2 and Akt, as well as increased cell proliferation. Five unknown and 13 previously identified proteins were differentially expressed in response to EGF. EGF treatment increased the expression of several structural proteins of epithelial cells, such as cytokeratin 19 and plakoglobin, whereas vimentin, the intermediate filament protein of mesenchymal cells, and non-muscle myosin alkali chain isoform 1, decreased. Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 factor, which promotes epithelial cell proliferation, and hemoglobin alpha I & II also increased, whereas cyclin A1, immunoglobulin heavy chain, apolipoprotein A1, 5,10-ethylenetetrahydrofolated reductase (5,10-MTHFR), angiotensin-converting enzyme 2 (ACE2), co-lipase II precursor, and NAD<SUP>+</SUP> isocitrate dehydrogenase (NAD<SUP>+</SUP> IDH) alpha chain proteins decreased. Our results show that EGF stimulates proliferation of pancreatic epithelial cells by simultaneously activating the MAPK and PI-3K pathways. HnRNP A2/B1, hemoglobin, cyclin A1, and ACE2 may play roles in the proliferation of epithelial cells in response to EGF. © 2005 Wiley-Liss, Inc.</P>