Epidemioclinical Feature of Early-Onset Colorectal Cancer at-Risk for Lynch Syndrome in Central Iran

Zeinalian, Mehrdad,Hashemzadeh-Chaleshtori, Morteza,Akbarpour, Mohammad Javad,Emami, Mohammad Hassan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Background: Colorectal cancer (CRC) is becoming one of the most complicated challenges of human health, particularly in developing countries like Iran. In this paper, we try to characterize CRC cases diagnosed < age 50 at-risk for Lynch syndrome within central Iran. Materials and Methods: We designed a descriptive retrospective study to screen all registered CRC patients within 2000-2013 in Poursina Hakim Research Center (PHRC), a referral gastroenterology clinic in central Iran, based on being early-onset (age at diagnosis ${\leq}50years$) and Amsterdam II criteria. We calculated frequencies and percentages by SPSS 19 software to describe clinical and family history characteristics of patients with early-onset CRC. Results: Overall 1,659 CRC patients were included in our study of which 413 (24.9%) were ${\leq}50years$ at diagnosis. Of 219/413 successful calls 67 persons (30.6%) were reported deceased. Family history was positive for 72/219 probands (32.9%) and 53 families (24.2%) were identified as familial colorectal cancer (FCC), with a history of at-least three affected members with any type of cancer in the family, of which 85% fulfilled the Amsterdam II Criteria as hereditary non-polyposis colorectal cancer (HNPCC) families (45/219 or 20.5%). Finally, 14 families were excluded due to proband tumor tissues being unavailable or unwillingness for incorporation. The most common HNPCC-associated extracolonic-cancer among both males and females of the families was stomach, at respectively 31.8 and 32.7 percent. The most common tumor locations among the 31 probands were rectum (32.3%), sigmoid (29.0%), and ascending colon (12.9%). Conclusions: Given the high prevalence of FCC (~1/4 of early-onset Iranian CRC patients), it is necessary to establish a comprehensive cancer genetic counseling and systematic screening program for early detection and to improve cancer prognosis among high risk families.

Tripropylammonium Fluorochromate (TriPAFC): A Convenient New Reagent for Oxidation of Organic Substrates

Ghammamy, S.,Hashemzadeh, A. Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8

Tripropylammonium Fluorochromate (TriPAFC): A Convenient New Reagent for Oxidation of Organic Substrates

S. Ghammamy*,A. Hashemzadeh 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.8

Controlled Synchronization of Nonlinear Teleoperation in Task-space with Time-varying Delays

Amir Zakerimanesh,Farzad Hashemzadeh,Ali Torabi,Mahdi Tavakoli 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.8

This paper introduces a novel control framework for bilateral teleoperation system with the redundant remote robot to ensure the end-effectors’ position tracking while satisfying a sub-task control such as obstacle avoidance in the presence of the nonlinear dynamics for the manipulators and bounded time-varying delays in the communication channels. The asymptotic stability of the closed-loop dynamics is studied using a Lyapunov-Krasovskii functional under conditions on the controller parameters and the maximum values of time-varying delays. Simulation and experimental results are provided to validate the theoretical findings.

The adsorption of malachite green (MG) as a cationic dye onto functionalized multi walled carbon nanotubes

Amir Hossein Mahvi,Mohammad Shirmardi,Bayram Hashemzadeh,Abolfazl Naeimabadi,Ghasem Hassani,Mehdi Vosoughi Niri 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.8

Synthetic dyes are widely used by several industries to color their products. The discharge of colored wastewater into the hydrosphere causes serious environmental problems. We used functionalized multi wall carbon nanotubes as an adsorbent for the adsorption of cationic dye, malachite green, from aqueous solution. Based on information provided by the Iranian Research Institute of Petroleum Industry, carbon nanotubes are produced using a chemical vapor deposition (CVD) technique. These as-received MWCNTs were functionalized by acid treatment. The remaining dye concentration was read by UV-visible absorption spectroscopy at maximum adsorption wavelength. The effect of different operational parameters such as contact time, pH of solution, adsorbent dose and initial dye concentration were studied. The results showed that by increasing of contact time, pH and adsorbent dose the removal of dye increased,but by increasing initial dye concentration, the removal efficiency decreased. Adsorption isotherms and kinetics behavior of f-MWCNTs for removal of malachite green was analyzed, and fitted to various existing models. The experimental data were well correlated with the Langmuir isotherm with a maximum adsorption capacity (qm) and regression coefficient (R2) of 142.85 mg/g and 0.997, respectively. The results of this study indicate that functionalized multi wall carbon nanotubes can be used as an effective adsorbent for the removal of dyes.

MicroRNA-183 Family in Inner Ear: Hair Cell Development and Deafness

Mohammad Reza Mahmoodian sani,Morteza Hashemzadeh Chaleshtori,Massoud Saidijam,Mohammad-Saeid Jami,Payam Ghasemi-Dehkordi 대한청각학회 2016 Journal of Audiology & Otology Vol.20 No.3

miRNAs are essential factors of an extensively conserved post-transcriptional process controlling gene expression at mRNA level. Varoius biological processes such as growth and differentiation are regulated by miRNAs. Web of Science and PubMed databases were searched using the Endnote software for the publications about the role miRNA-183 family in inner ear: hair cell development and deafness published from 2000 to 2016. A triplet of these miRNAs particularly the miR-183 family is highly expressed in vertebrate hair cells, as with some of the peripheral neurosensory cells. Point mutations in one member of this family, miR-96, underlie DFNA50 autosomal deafness in humans and lead to abnormal hair cell development and survival in mice. In zebrafish, overexpression of the miR-183 family induces extra and ectopic hair cells, while knockdown decreases the number of hair cell. The miR-183 family (miR-183, miR-96 and miR-182) is expressed abundantly in some types of sensory cell in the eye, nose and inner ear. In the inner ear, mechanosensory hair cells have a robust expression level. Despite much similarity of these miRs sequences, small differences lead to distinct targeting of messenger RNAs targets. In the near future, miRNAs are likely to be explored as potential therapeutic agents to repair or regenerate hair cells, cell reprogramming and regenerative medicine applications in animal models because they can simultaneously down-regulate dozens or even hundreds of transcripts.

Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for Characterizing Microsatellite Instability in Persian Lynch Syndrome Patients

Farahani, Najmeh,Nikpour, Parvaneh,Emami, Mohammad Hassan,Hashemzadeh, Morteza,Zeinalian, Mehrdad,Shariatpanahi, Seyed Shervin,Salehi, Rasoul Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Background: Colorectal malignancies with high microsatellite instability (MSI-H), either hereditary (Lynch syndrome) or sporadic, demonstrate better prognosis and altered response to 5FU chemotherapy. It is now recommended to perform MSI testing for all new cases of colorectal cancer regardless of being categorized as hereditary or sporadic. For MSI detection, immunohistochemistry or PCR-based protocols using a cohort of various sets of STR markers are recommended. Here we aimed to evaluate a simplified protocol using just a single STR marker, MT1XT20 mononucleotide repeat, for detection of MSI in Lynch syndrome patients. A Promega five-marker MSI testing panel and immunohistochemistry (IHC) were used as the gold standard in conjunction with MT1XT20. Materials and Methods: Colorectal patients with a positive history of familial cancers were selected by evaluating medical records. Based on Amsterdam II criteria for Lynch syndrome 20 families were short listed. DNA was extracted from formalin fixed paraffin embedded tumour and adjacent normal tissues resected from the index case in each family. Extracted DNA was subjected to MT1XT20 mononucleotide marker analysis and assessment with a commercially available five marker MSI testing kit (Promega, USA). IHC also was performed on tissue sections and the results were compared with PCR based data. Results: Eight (40%), seven (35%) and five (25%) cases were MSI positive using with the Promega kit, IHC and MT1XT20, respectively. Among the markers included in Promega kit, BAT26 marker showed instability in all 8 samples. NR24 and NR21 markers showed instability in 7 (87.5%), and BAT25 and MONO 27 in 6 (75%) and 5 (62.5%). Conclusions: Although MT1XT20 was earlier reported as a valid standalone marker for MSI testing in CRC patients, we could not verify this in our Iranian patients. Instead BAT26 among the markers included in Promega MSI testing kit showed instability in all 8 MSI-H CRC samples. Therefore, it seems BAT26 could act well as a single marker for MSI testing in Iranian CRC patients.

Verification of Scherrer Formula for Well-Shaped Poly(3-hexylthiophene)- Based Conductive Single Crystals and Nanofibers and Fabrication of Photovoltaic Devices from Thin Film Coating

Sahar Zenoozi,Samira Agbolaghi,Elaheh Poormahdi,Mahdi Hashemzadeh-Gargari,Mojgan Mahmoudi 한국고분자학회 2017 Macromolecular Research Vol.25 No.8

Scherrer formula was verified for the single crystals and nanofibers prepared from poly(3-hexylthiophene) (P3HT)-based homopolymers (non-hairy) and block copolymers (hairy) with polystyrene, poly(methyl methacrylate), and poly(ethylene glycol) in toluene, xylene, and anisole. Despite the fact that he single crystals were grown in very thicker dimensions (57-139 nm) compared to the nanofibers (2-8 nm), the data obtained in the hexyl chains direction from atomic force microscopy and grazing wide angle X-ray scattering using Scherrer formula had a high consistency for both hairy and non-hairy single crystals and nanofibers grown from toluene. This adaption was detected only in toluene for the single crystals, however, in toluene and xylene for the nanofibers. In a better solvent employed in the growth environment, both single crystals and nanofibers were composed of lower number of discrete crystallites in their thickness and longitude. The other effective parameters on the crystallite sizes inside the single crystals and nanofibers in both (100) and (020) directions were the crystallization temperature, the molecular weight of rigid P3HT block, and the end coily blocks. The longer P3HT backbones reflected the thinner and shorter crystallites assembled inside the single crystals (25-87 nm in hexyl chains and 48-166 nm in π-π stacking directions) and nanofibers (2-7 nm in hexyl chains and 2-25 nm in π-π stacking directions). Finally, thin films of pre-developed structures were employed as active layers in P3HT:phenyl-C71-butyric acid methyl ester (PC71BM) photovoltaic cells, and the device characteristics were investigated.

Genetics of Hearing Loss in North Iran Population: An Update of Spectrum and Frequency of GJB2 Mutations

Mahbobeh Koohiyan,Fatemeh Azadegan-Dehkordi,Farideh Koohian,Morteza Hashemzadeh-Chaleshtori 대한청각학회 2019 Journal of Audiology & Otology Vol.23 No.4

Diagnosis of pre-lingual hearing loss (HL) is difficult owing to the high number of genes responsible. The most frequent cause of HL is DFNB1 due to mutations in the GJB2 gene. It represents up to 40% of HL cases in some populations. In Iran, it has previously been shown that DFNB1 accounts for 16-18% of cases but varies among different ethnic groups. Here, we reviewed results from our three previous publications and data from other published mutation reports to provide a comprehensive collection of data for GJB2 mutations and HL in northern Iran. In total, 903 unrelated families from six different provinces, viz., Gilan, Mazandaran, Golestan, Ghazvin, Semnan, and Tehran, were included and analyzed for the type and prevalence of GJB2 mutations. A total of 23 different genetic variants were detected from which 18 GJB2 mutations were identified. GJB2 mutations were 20.7% in the studied northern provinces, which was significantly higher than that reported in southern populations of Iran. Moreover, a gradient in the frequency of GJB2 mutations from north to south Iran was observed. c.35delG was the most common mutation, accounting for 58.4% of the cases studied. This study suggests that c.35delG mutation in GJB2 is the most important cause of HL in northern Iran.

BMI1 and TWIST1 Downregulated mRNA Expression in Basal Cell Carcinoma

Rajabpour, Fatemeh Vand,Raoofian, Reza,Youssefian, Leila,Vahidnezhad, Hassan,Shahshahani, Mostafa Mirshams,Fathi, Hamidreza,Noormohammadpour, Pedram,Hesari, Kambiz Kamyab,Hashemzadeh-Chaleshtori, Mort Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: BMI1, TWIST1 and SNAI2/SLUG have been implicated in aggressive behavior of squamous cell carcinoma (SCC) and melanoma and BMI1 expression could identify subtypes of Merkel cell carcinoma (MCC). However, BMI1, TWIST1 and SNAI2 expression levels in basal cell carcinomas (BCCs) have not been elucidated. We hypothesized BCC could be a good model system to decipher mechanisms which inhibit processes that drive tumor metastasis. The aim of this study was to examine the mRNA expression level of BMI1, TWIST1, and SNAI2 in BCCs. Materials and Methods: Thirty-five fresh non-metastatic BCC tissue samples and seven fresh normal skin tissue samples were evaluated by real-time RT-PCR. Results: BMI1 and TWIST1 demonstrated marked down-regulation (p<0.00l, p=0.00l respectively), but SNAI2 showed no significant change (p=0.12). Conclusions: Previous literature has clearly demonstrated a positive association between BMI1 and TWIST1 expression and metastatic BCC, aggressive SCC and melanoma. Here, we demonstrated a negative association between BMI1 and TWIST1 mRNA expression level and BCC.