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- 저자 : Hasan Azad (검색결과 5 건)
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Hassan Gholizadeh,Azade Ghorbani‑HasanSaraei,Hasan Tahermansouri,Seyed‑Ahmad Shahidi 한국탄소학회 2019 Carbon Letters Vol.29 No.3
The carboxylated multi-walled carbon nanotubes (MWCNTs–COOH) were used as adsorbent for the separation of flavonoids (naringin and rutin) from bitter orange peel. The influence of the parameters such as, pH values, contact time, and desorption conditions was investigated. The samples were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, derivative thermogravimetric, scanning electron microscopy, UV–Vis spectroscopy, and high-performance liquid chromatography. After separation and desorption process, the eluent was injected for chromatography analysis. Under the optimal conditions, experimental results showed that the extraction efficiency of rutin was higher than naringin and other compounds. Moreover, the desorption percentage of flavonoids was calculated 83.6% after four cycles. This research confirmed that this method for separation of flavonoids is simple and less cost. In addition, the separated flavonoids can be used as antioxidant for the future applications.
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( Abul Kalam Azad ),( Asraful Jahan ),( Mahbub Hasan ),( Takahiro Ishikawa ),( Yoshihiro Sawa ),( Hitoshi Shibata ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.11
The Sec61α subunit is the core subunit of the protein conducting channel which is required for protein translocation in eukaryotes and prokaryotes. In this study, we cloned a Sec61α subunit from Penicillium ochrochloron (PoSec61α). Sequence and 3D structural model analysis showed that PoSec61α conserved the typical characteristics of eukaryotic and prokaryotic Sec61α subunit homologues. The pore ring known as the constriction point of the channel is formed by seven hydrophobic amino acids. Two methionine residues from transmembrane α-helice 7 (TM7) contribute to the pore ring formation and projected notably to the pore area and narrowed the pore compared with the superposed residues at the corresponding positions in the crystal structures or the 3D models of the Sec61α subunit homologues in archaea or other eukaryotes, respectively. Results reported herein indicate that the pore ring residues differ among Sec61α subunit homologues and two hydrophobic residues in the TM7 contribute to the pore ring formation. [BMB reports 2011; 44(11): 719-724]
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Apoptosis Induction by Ocimum sanctum Extract in LNCaP Prostate Cancer Cells
Sivanesan Dhandayuthapani,Hasan Azad,Appu Rathinavelu 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.7
Tulsi (Ocimum sanctum Linn), commonly known as ‘‘holy basil,’’ has been used for the treatment of a wide range of ailments in many parts of the world. This study focuses on apoptosis-inducing ability of tulsi extract on prostate cancer cells. For this purpose LNCaP prostate cancer cells were treated with different concentrations of 70% ethanolic extract of tulsi (EET) and then the cytotoxicity was determined after 24 and 48 h. After treatment with EET externalization of phosphatidyl serine (PS) from the inner membrane to outer leaflet of the plasma membrane was clearly evidenced by the results obtained from both flow cytometry analysis with Annexin V-FITC and pSIVA-IANBD binding fluorescence microscopy assay. Depolarization of the mitochondrial membrane potential was also evidenced by the presence of 5,5',6,6'-tetrachlolo-1,1',3,3'-tetraethyl benzimedazolyl carbocyanine iodide ( JC-1) monomeric form in the EET-treated cells that emitted the green fluorescence when compared with the control cells that emitted the red fluorescence due to aggregation of JC-1. Furthermore, the level of poly (ADP-ribose) polymerase (PARP) cleavage and Bcl-2 were determined using western blot analysis. When compared to the control cells the level of cleaved PARP was found to be higher with a concomitant decrease in the Bcl-2 level after 24 h of treatment of cells with EET. In addition, treatment with EET significantly elevated the activities of caspase-9 and caspase-3 in LNCaP cells compared with the control. Also, after 48 h of treatment all doses used in this study showed clear fragments of DNA, which is one of the hallmarks of apoptosis. Taken together, our findings suggest that, EET can effectively induce apoptosis in LNCaP cells via activation of caspase-9 and caspase-3 that can eventually lead to DNA fragmentation and cell death.
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Gholizadeh Hassan,Ghorbani-HasanSaraei Azade,Tahermansouri Hasan,Shahidi Seyed-Ahmad 한국탄소학회 2019 Carbon Letters Vol.29 No.3
The carboxylated multi-walled carbon nanotubes (MWCNTs–COOH) were used as adsorbent for the separation of flavonoids (naringin and rutin) from bitter orange peel. The influence of the parameters such as, pH values, contact time, and desorption conditions was investigated. The samples were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, derivative thermogravimetric, scanning electron microscopy, UV–Vis spectroscopy, and high-performance liquid chromatography. After separation and desorption process, the eluent was injected for chromatography analysis. Under the optimal conditions, experimental results showed that the extraction efficiency of rutin was higher than naringin and other compounds. Moreover, the desorption percentage of flavonoids was calculated 83.6% after four cycles. This research confirmed that this method for separation of flavonoids is simple and less cost. In addition, the separated flavonoids can be used as antioxidant for the future applications.
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Md. Iftekharul AMIN,Aumit AHSAN,Mahmud Al MUKTADIR,Muntasir AZAD,Razib Hasan Bin REZANUR 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.12
A prerequisite of a sound financial system is effective channeling of financial resources to efficient users; hence maximizing economic and societal welfare. To that end, the prevalence of bad loans in banks in emerging economies is a major policy concern. In an attempt to add to the growing body of literature explaining the interrelationship between macroeconomic and firm-specific factors, and non-performing loans (NPL), this paper examines data from 24 scheduled commercial banks in Bangladesh from 2008 to 2019. Macroeconomic factors as well as firm-specific factors related to profitability, capital strength, and efficiency are considered. Panel data regression analysis is performed to estimate pooled OLS, fixed effects, and random effects models. Following the necessary testing, it was found that the fixed effects model with robust standard error is appropriate. Results show that return on assets and inflation have a negative influence on NPL, but GDP growth has a favorable impact. The paper concludes by asserting that the evidence supports similar findings from studies both in Bangladesh and elsewhere and it is noted that a combination of these macroeconomic and firm-specific factors explains only a small portion of the total variation in NPL
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