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The Limitation of Making Hepatic Fibrosis in NAFLD Animal Model
( Han Seul Park ),( Jae Young Jang ),( Soung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( Boo Sung Kim ),( So Young Jin ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Animal models of NAFLD give crucial information, not only pathogenesis of NAFLD but also therapeutic effects of various agents. We investigated the degree of steatosis, inflammation and fibrosis of the liver obtained from three animal models of NAFLD with different mouse species and diets. Methods: Group1: The mice were fed a control diet. Group2: The gene responsible for the production of leptin deficient ob/ob mice fed a 60% fat diet. Group3: The leptin receptor deficient db/db mice were fed a diet deficient in methionine and choline (MCD) diet. Group4: The leptin receptor deficient db/db mice were fed a 60% fat + 2.5% cholesterol diet and drinking water (55% fructose, 45% glucose w/w). Change of body weight was observed and blood was collected before sacrifice. After being sacrificed, the liver tissues were collected and fixed in formalin. Histological evaluation was conducted by blindly pathologist. Results: The body weight in group1 & 2 & 4 (control & high-fat-diet fed mice) were increased during the study, whereas group3 (MCD feeding mice) showed weight loss for unclear reasons. Liver histology showed no significant difference in hepatic steatosis and inflammation among the three groups (group2-4). Meanwhile, liver fibrosis was slightly more frequent in group3 than that in groups2 and 4. However, the maximum degree of fibrosis in group3 was Ib. The level of liver enzyme showed no significant difference, whereas the level of triglyceride was significantly increased in group4 (TG: 30.8±3.2 (group1), 31.8±4.9 (group2), 32.8±1.7 (group3), 141±15.20 (group4) (p < 0.001) (mg/dL)). Conclusions: The results show that the three animal models are thought to induce hepatic steatosis and inflammation very well, but the induction of hepatic fibrosis is still considered to be limited. A better animal model development or integration model would be needed.
Over Expression of Aminopeptidase N has Negative Effect on Boar Fertility
Won-Ki Pang,Kyu-Ho Kang,Ki-Uk Kim,Ki-Jin Kwon,Sae-Han Kang,Amena Khatun,Do-Yeal Ryu,Md Saidur Rahman,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Artificial insemination is a commonly used technology in the porcine industry that directly linked to the genetic upgradation of future progeny and profitable farm management. Therefore, accurate prediction of boar fertility is a matter of paramount importance. Although prediction of semen/sperm fertility using conventional analysis provides a preliminary assumption of male fertility, its practical value is limited. Protein content in the cell provides an important and fascinating insight to the dynamics of cell function. Therefore, the study of protein function in spermatozoa and their corresponding relationship with male fertility might be an excellent alternative tool for more accurate prediction of male fertility. Recently we demonstrated that aminopeptidase N (APN) activity is negatively correlated with motility and fertility of mice spermatozoa. Here, we investigated whether APN is also correlated with the fertility of boar. Spermatozoa were collected from high and low fertility boar based on their field fertility data (average litter size 13.2±0.06 and 11.1±0.14, respectively). Simultaneously, APN levels were examined by Western blotting using the corresponding antibody. In addition, spermatozoa from both fertility groups were examined by conventional semen analysis such as computer assisted sperm analysis (CASA) and chlorotetracycline (CTC) staining. Our data showed a significant higher APN expression in low fertility boar spermatozoa compare to the fertile counterpart (p<0.05). Interestingly, no significant difference was noticed in motility, motion kinematics, and capacitation status between both groups. Based on this findings, it is tempting to speculate that APN activity might directly correlate with boar fertility. Therefore, APN activity in spermatozoa is more accurate and sensitive biomarker for the detection of boar fertility then conventional semen analysis. However, further studies and needed to confirm our initial findings
Rethinking the US Presidential Election: Feminism and Big Data
Sae Won CHUNG,Han Woo PARK 한국콘텐츠학회(IJOC) 2021 International Journal of Contents Vol.17 No.4
The 2020 US Presidential Election was a highly-anticipated moment for our global society. During the election period, the most intriguing issue was who would be the winner-Trump or Biden? Among the possible main themes of the 2020 election, from the COVID-19 pandemic to racism, this study focused on feminism (‘women’) as a main component of Biden’s victory. To explore the character of Biden’s supporters, this paper focused on internet spaces as a source of public opinion. To guide the data analysis, this study employed four indices from empirical studies on Big Data analytics: issue salience, attention diversity, emotional mentioning, and semantic cohesion. The main finding of this study was that the representative keyword ‘women’ appeared more prevalently within content related to Biden than Trump, and the keyword pairs indicated that female voters were the main reason for Trump’s failure but the root cause of Biden’s victory. The results of this study indicated the role of the internet as a forum for public opinion and a fountain of political knowledge, which requires more rigorous investigation by researchers.
The Inhibitory Effect of Lorcaserin on Non Alcoholic Fatty Liver Disease in Animal Model
( Han Seul Park ),( Jae Young Jang ),( Seoung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( Boo Sung Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Nonalcoholic fatty liver disease (NAFLD) is characterized by a wide spectrum of liver damage spanning steatosis, nonalcoholic steatohepatitis, and liver cirrhosis. The aim of this study is to investigate the efficacy of lorcaserin on NAFLD in animal model. Methods: The leptin receptor deficient db/db mice and control mice (db/m) were fed a diet deficient in methionine and choline (MCD diet) and control diet for 8, respectively. Twenty mice were divided into 3 groups. The first group was fed a control diet without treatment and referred as the control group. The second group was administered with MCD diet +0.7% DMSO. The third group was administered with MCD diet +0.7% DMSO +5mg/ml of lorcaserin. Change of body weight was observed and blood was collected before sacrifice. After being sacrificed, the liver tissues were collected and fixed in formalin. Histological evaluation was evaluated by blindly pathologist. Results: The body weight of control mice was increased during the study, whereas feeding db/db mice MCD diets for 8 weeks significantly reduced in body weight. Lorcaserin treated group was associated with more rapid body weight loss compared with DMSO-treated controls. MCD diet induced excessive fat accumulation, inflammation, and some fibrosis. Liver enzyme and triglyceride were improved in lorcaserin-treated group compared with DMSO-treated control (DMSO vs lorcaserin: AST 411.3 ±40.26 vs 304.7 ±28.88 (U/L), ALT 544.8 ±38.7 vs 434.5 ±29.68 (U/L), triglyceride 31.8 ±2.02 vs 26 ±1.58 (mg/dL)). Liver histopathology showed that the fat accumulation and inflammatory cell infiltration were decreased in MCD diet +lorcaserin-treated mice compared with MCD diet +DMSO-treated controls. Conclusions: These results showed beneficial effects of lorcaserin against excessive fat accumulation and inflammation as well as liver enzyme. Therefore, our findings indicate that lorcaserin could be contributing to the decline of progression of nonalcoholic fatty liver disease.
RNA editing in <i>RHOQ</i> promotes invasion potential in colorectal cancer
Han, Sae-Won,Kim, Hwang-Phill,Shin, Jong-Yeon,Jeong, Eun-Goo,Lee, Won-Chul,Kim, Keon Young,Park, Sang Youn,Lee, Dae-Won,Won, Jae-Kyung,Jeong, Seung-Yong,Park, Kyu Joo,Park, Jae-Gahb,Kang, Gyeong Hoon The Rockefeller University Press 2014 The Journal of experimental medicine Vol.211 No.4
<P>RNA editing can increase RNA sequence variation without altering the DNA sequence. By comparing whole-genome and transcriptome sequence data of a rectal cancer, we found novel tumor-associated increase of RNA editing in <I>ras homologue family member Q</I> (<I>RHOQ</I>) transcripts. The adenosine-to-inosine (A-to-I) editing results in substitution of asparagine with serine at residue 136. We observed a higher level of the <I>RHOQ</I> RNA editing in tumor compared with normal tissue in colorectal cancer (CRC). The degree of RNA editing was associated with RhoQ protein activity in CRC cancer cell lines. RhoQ N136S amino acid substitution increased RhoQ activity, actin cytoskeletal reorganization, and invasion potential. <I>KRAS</I> mutation further increased the invasion potential of RhoQ N136S in vitro. Among CRC patients, recurrence was more frequently observed in patients with tumors having edited <I>RHOQ</I> transcripts and mutations in the <I>KRAS</I> gene. In summary, we show that RNA editing is another mechanism of sequence alteration that contributes to CRC progression.</P>
Methylation and microsatellite status and recurrence following adjuvant FOLFOX in colorectal cancer
Han, Sae‐,Won,Lee, Hyun‐,Jung,Bae, Jeong Mo,Cho, Nam‐,Yun,Lee, Kyung‐,Hun,Kim, Tae‐,Yong,Oh, Do‐,Youn,Im, Seock‐,Ah,Bang, Yung‐,Jue,Jeong, Seung‐ Wiley Subscription Services, Inc., A Wiley Company 2013 International journal of cancer: Journal internati Vol.132 No.9
<P><B>Abstract</B></P><P>The prognostic impact of CpG island methylator phenotype (CIMP) and microsatellite instability (MSI) on the treatment outcome of colon cancer patients receiving adjuvant 5‐fluorouracil/leucovorin/oxaliplatin (FOLFOX) is unclear. We investigated CIMP and MSI status in colorectal cancer patients treated with adjuvant FOLFOX. Stages II and III sporadic colorectal cancer patients who underwent curative resection followed by adjuvant FOLFOX were included. Eight CpG island loci (CACNA1G, CRABP1, IGF2, MLH1, NEUROG1, CDKN2A (p16), RUNX3 and SOCS1) and five microsatellite markers were examined. Disease‐free survival (DFS) was analyzed according to CIMP and MSI status. A total of 322 patients were included: male/female 192/130, median age 61 years (range 30–78), proximal/distal location 118/204 and Stages II/III 43/279. CIMP status was high in 25 patients (7.8%) and 21 patients (6.5%) had MSI‐high tumor. CIMP/MSI status was not significantly associated with DFS: 3‐year DFS 100% in CIMP(−)/MSI(+), 84% in CIMP(−)/MSI(−), 82% in CIMP(+)/MSI(−) and 75% in CIMP(+)/MSI(+) (<I>p</I> = 0.33). Results of exploratory analysis showed that concurrent methylation at NEUROG1 and CDKN2A (p16) was associated with shorter DFS: 3‐year DFS 69% in NEUROG1(+)/CDKN2A (p16)(+) versus 87% in NEUROG1(−)/CDKN2A (p16)(−) (<I>p</I> = 0.006). In conclusion, concurrent methylation of NEUROG1 and CDKN2A (p16) is associated with recurrence following adjuvant FOLFOX in Stages II/III colorectal cancer.</P>
TAK1 Mediates Lipopolysaccharide-induced RANTES Promoter Activation in BV-2 Microglial Cells
Sae-Byeol Jang,Jonghwa Won,Hongtae Kim,Jaeyoung Kim,Kweon-Haeng Lee,Hoon Han,Hyoung Kyun Rha,Chang Rak Choi 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.1
Lipopolysaccharide (LPS), a part of the outer mem-brane of gram-negative bacteria activates the expres-sion of the regulated upon activation, normal T cell expressed and secreted (RANTES), which plays an important role in the chemo-attraction of leukocytes during the inflammatory response. Recently, we found that LPS-induced RANTES production is mediated by the activation of NF-kB, but, the upstream regulatory mechanism involved in mediating this NF-kB activa-tion was unclear. In this study, we investigated signal transducing molecules that mediate LPS-induced RANTES promoter activation and found the follow-ings. First, LPS activates the RANTES gene promoter through NF-kB binding sites. Second, the expression of dominant negative mutants of TGF-b-activated kinase1 (TAK1) and NF-kB-inducing kinase (NIK), blocked the LPS-induced transcriptional activation of RANTES promoter. Moreover, the overexpression of TAK1 along with TAK1-binding protein 1 (TAB1), or NIK stimulated the transcriptional activation of RANTES in the absence of external stimuli. Third, we showed that endogenous TAK1 is phosphorylated by LPS stimulation, and that the association between TAK1 and tumour necrosis factor receptor-associated factor 6 (TRAF6) is constitutive and not induced by LPS treatment. These results indicate that NF-kB me-diates LPS-triggered RANTES induction and that TAK1 as well as NIK, as NF-kB activators participates in LPS-triggered RANTES induction.