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Effects of melatonin on development of early mouse embryos treated with Lipopolysaccharide
Haeun Park,Sohyeon Moon,Byeongseok Kim,Siyoung Lee,Semi Hwang,Youngsok Choi 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Lipopolysaccharide (LPS) is an endotoxin factor present in the cell wall of Gram-negative bacteria and induces various immune responses to severe infections. Recent studies have been reported that LPS induces cellular stress in various cell lines, oocytes, and blastocyst. Antioxidants are used as one of the ways to overcome the LPS-mediated cytotoxic effect, but the effect of co-administration with LPS and melatonin (N-acetyl-5-methoxytryptamine) on early embryonic development has not yet been confirmed. Melatonin is a regulation hormone of circadian rhythm and a powerful antioxidant. It has been known that melatonin has an effective function in scavenging oxygen free radicals. Therefore, we cultured mouse embryos until the blastocyst stage (day 5) in the in vitro culture medium containing two reagents, we evaluated the development rate and the level of reactive oxygen species (ROS) in the embryos. First, we cultured embryos in medium at concentrations of 0 (control), 12.5, 25, 50, and 100 μg/mL LPS. As a result, morula development rate was inhibited in the high concentration of 100 μg/mL LPS group (41.4 ± 5.89% vs control 65.6 ± 2.04%; p<0.05), and blastocyst development rate was decreased from the concentration of 12.5 μg/mL LPS group (35.0 ± 7.33%) compared to the control group (61.3 ± 1.83%; p<0.01). In the subsequent experiment, 12.5ug/mL LPS was selected as optimal concentration and used. Next, to confirm the effect on melatonin of embryonic development, embryos were cultured in medium of melatonin 10-8, 10-7, and 10-6 M concentrations. There was no statistical difference in embryo development rate in all melatonin-treated groups, and no toxic effects were observed. Co-administration with LPS and melatonin (10-7 or 10-6 M) significantly increased the blastocyst development rate (MT 10-7 M 52.8 ± 4.96%, MT 10-6 M 59.8 ± 9.20%; P<0.01) inhibited by LPS (LPS 34.6 ± 4.22% vs control 70.4 ±3.96%; p<0.01). ROS levels were measured during the two-cell and the blastocyst development stage. In both stages, ROS level of embryos exposed to LPS had elevated than control group, and the ROS level of embryos in co-administration group was significantly decreased. In conclusion, this experiment demonstrated the protective effect of melatonin on the inhibition of embryonic development induced by LPS. These results suggest that melatonin may possibly be a potential infertile therapeutic agent for women exposed to bacterial infections.
Park Haeun,박찬희,유승현 한국전문물리치료학회 2022 한국전문물리치료학회지 Vol.29 No.3
Background: The presence of the lumbopelvic-hip neuromuscular chain is essential for dynamic spinal stabilization; its therapeutic effects on dynamic movements of the distal extremity segment and underpinning motor mechanism remain unknown and warrant further study on participants with low back pain (LBP). Objects: We aim to compare the effects of the broken chain exercise (BCE) and connected chain exercise (CCE) on electromyography (EMG) amplitude and onset time in participants with and without LBP. Methods: Randomized controlled clinical trial. A convenience sample of 40 nonathletic participants (mean age: 24.78 ± 1.70) with and without LBP participated in this study. All participants underwent CCE for 30 minutes, 30-minute daily. We measured EMG amplitude and onset times on bilateral erector spinae (ES), gluteus maximus (GM), hamstring (HAM), transverse abdominis (TrA), internal oblique (IO), and external oblique (EO) during the prone hip extension (PHE) test before and after the BCE and CCE. We used multivariate analysis of variance (MANOVA) to analyze the amplitude and onset time difference between exercises (BCE and CCE) and Pearson’s correlations to determine any synergistic relationship among the HAM, GM, bilateral TrA/IO, and ES muscles. The statistical analyses were used at p < 0.05. Results: MANOVA showed that CCE was more decreased on EMG amplitude in HAM and bilateral ES, while increased GM and contralateral TrA/IO than BCE (p < 0.05). MANOVA EMG onset time data analyses revealed that the main effect of the conditions was significant for all HAM, GM, and bilateral ES muscles, whereas the main effect for the group was significant only for GM and contralateral ES in healthy and LBP groups. Pearson’s correlation coefficient was computed to assess the relationship between BCE and CCE on dependent variables. In most of the muscles, there was a strong, positive correlation between the two variables, and there was a significant relationship (p < 0.001). Conclusion: CCE produced more effective and coordinated core stabilization and motor control mechanism in the lumbopelvic-hip muscles in participants with and without LBP during PHE than BCE.
Park Haeun,Chang Joon Young,Hwang Jongseok,Lee Young Hee,유승현 한국전문물리치료학회 2022 한국전문물리치료학회지 Vol.29 No.3
Background: The PyeongChang 2018 Winter Paralympic Games (WPG) being one of the most successful Paralympic Games (PG) in modern athletic world history, hosted the largest number of elite athletes representing 49 National Paralympic Committees (NPCs). Objects: The present investigation highlighted the demographic and clinical characteristics of injured athletes and non-athletes and the physiotherapy services provided during the PyeongChang 2018 WPG. Methods: Prospective descriptive epidemiology study, in which the study group comprised of 201 participants (51 athletes and 150 non-athletes) who were admitted to and utilized the polyclinic physiotherapy service of 2018 PyeongChang WPG in Physiotherapy Department of Paralympic Village from March 1, 2018 to March 20, 2018. Results: Qualitative frequency analysis of injury type demonstrated highest number of chronic injuries (51%, n = 100) in athletes and non-athletes. Anatomical injury site analysis revealed that the spine and shoulder areas were affected with equal frequency for athletes (54.9%, n = 14), whereas for non-athletes, the frequencies of spine and shoulder area injuries were 36.7% (n = 55) and 26% (n = 39), respectively. The Pyeongchang WPG showed a high rate of athletes visiting the physiotherapy service during the pre-competition period (33.3%, n = 50), which may have led to smaller incidence rate of traumatic injury. The physiotherapy treatment service analysis demonstrated that manual therapy (35.4%, n = 230) was most commonly utilized, followed by transcutaneous electrical nerve stimulation/interference current therapy (TENS/ICT), therapeutic massage and therapeutic exercise. Conclusion: We established the importance of prophylactic and preventive physiotherapy services to reduce the risk of sports injuries during WPG.
Effect of LPS and melatonin on early development of mouse embryo
Park, Haeun,Jang, Hoon,Choi, Youngsok The Korean Society of Animal Reproduction and Biot 2022 한국동물생명공학회지 Vol.37 No.3
Lipopolysaccharide (LPS) is an endotoxin factor present in the cell wall of Gram-negative bacteria and induces various immune responses to infection. Recent studies have reported that LPS induces cellular stress in various cells including oocytes and embryos. Melatonin (N-acetyl-5-methoxytryptamine) is a regulatory hormone of circadian rhythm and a powerful antioxidant. It has been known that melatonin has an effective function in scavenging oxygen free radicals and has been used as an antioxidant to reduce the cytotoxic effects induced by LPS. However, the effect of melatonin on LPS treated early embryonic development has not yet been confirmed. In this study, we cultured mouse embryos in medium supplemented with LPS or/and melatonin up to the blastocyst stage in vitro and then evaluated the developmental rate. As a result of the LPS-treatment, the rate of blastocyst development was significantly reduced compared to the control group in all the LPS groups. Next, in the melatonin only treated group, there was no statistical difference in embryonic development and no toxic effects were observed. And then we found that the treatment of melatonin improved the rates of compaction and blastocyst development of LPS-treated embryos. In addition, we showed that melatonin treatment decreased ROS levels compared to the LPS only treated group. In conclusion, we demonstrated the protective effect of melatonin on the embryonic developmental rate reduced by LPS. These results suggest a direction to improve reproduction loss that may occur due to LPS exposure and bacterial infection through the using of melatonin during in vitro culture.
Haeun Kim,Jin Tae Hong,Mi Hee Park 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.3
Traditionally, Centella asiatica leaf extracts are used to treat neurodegenerative diseases in India. Centella asiatica is reportedly used to enhance memory and treat dementia, but its promoting effect on neural stem cell differentiation has not been studied yet. In the present study, we investigated whether or not Centella asiatica leaf extracts act on neuronal precursor cells and neuronal cell lines to induce neuronal differentiation, neurite outgrowth, and neuroprotection. The neurogenesis-promoting potential of Centella asiatica leaf extracts was determined by differentiation assay on neural stem cells isolated from mouse embryos and PC12 cell lines. To understand the contribution of specific neural cell types towards increase after Centella asiatica treatment, neural stem cells were differentiated into various neural subtypes and checked by Western blotting using neural cell lineage-specific antibody markers. Neuroprotective activity of Centella asiatica was analyzed in PC12 cells exposed to 100 μM of H2O2. Cell growth was analyzed by MTT assay while cell death was analyzed by Western blotting detection of apoptosis-related proteins. Cells treated with Centella asiatica had significantly longer primary and secondary neurites as well as a higher number of neurites per cell compared to control cells. Expression levels of TUBBIII, TH, NF, and BDNF increased upon Centella asiatica treatment, suggesting that Centella asiatica has a neurogenesis-promoting effect. Centella asiatica also inhibited oxidative stress-induced neural cell damage through regulation of apoptosis- and cell cycle-related proteins. Thus, leaf extracts of Centella asiatica might promote neurogenesis, neuroregeneration, and neuroprotection in the context of neurodegenerative diseases.
Analysis of volatile compounds and α-dicarbonyl compounds in coffee soaked with organic acids
Hyunbeen Park,Seungjun Lee,Jooyeon Park,Haeun Lee,Kwang-Geun Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In this study, volatile and α-dicarbonyl compounds (α-DCs) such as glyoxal, methylglyoxal (MGO) and diacetyl (DA) were analyzed in Arabica coffee. The Arabica coffee was soaked in 6 organic acid mixture (tartaric acid, malic acid, succinic acid, ascorbic acid, citric acid, citric acid, citric acid monohydrate) for different conditions (1, 3, 6, 12 hours). Volatile compounds were analyzed by solid phase microextraction (SPME) using gas chromatography-mass spectrometer detector (GC-MSD). α-DCs were analyzed by gas chromatography-nitrogen phosphorous detector (GC-NPD). The highest concentration of volatile compounds was detected in the sample prepared by succinic acid, 128.9% higher than the sample without soaking. The level of total α-DCs was 12.90 to 100.32 μg/mL. GO, MGO and DA ranged from 0.81 to 7.74 μg/mL, l 8.53 to 89.08 μg/mL and 0.43 to 6.74 μg/mL, respectively. Arabica coffee prepared with several organic acids has higher amounts of volatile compounds and lower α-DCs concentration than coffee without soaking (p<0.05). The results obtained in this study would be useful for increasing the flavor of Arabica coffee and reducing α-DCs.