천연한약재(목통, 삼릉, 치자) 추출물의 항산화효과 및 항균활성효과

김정균,강영미,엄광식,고영민,김태영 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.4

한약재로 많이 이용되고 있는 목통, 삼릉 및 치자의 항산화활성과 항균활성 정도를 검토하기 위해 한약재를 물, 70% methanol(MeOH) 및 100% MeOH로 추출한 후 각 추출물의 페놀화합물함량, 전자공여능, hydroxy radical 소거효과, reducing power 및 항균활성시험을 하였다. 그 결과 실험에 사용한 3가지 한약재 모두 항산화효과와 항균활성이 뛰어났으며, 특히 목통 70% MeOH 추출물이 그 효과가 가장 컸으며 다음이 100% MeOH, 물추출물 순이었다. Antioxidant activity and antimicrobial activity of medicinal plants extracts from 3 edible or medicinal plant were examined. The extracts from Akebia quinate Decaisne, Scirusfluviatilis A. Gray, Gardenia jasminoides for. grandiflora Makino showed comparatively strong antioxidative activity on test. Electron donatating abilities and hydroxy radical scavenging ablities of 70% methanol, 100% methanol extracts were higher than thoes of water extracts. Some extracts of medicinal plants showed antimicrobial effect, especially, the extracts showed antimicrobial effect in the 70% methanol higher than other extracts.

Anti-oxidative Properties of Lipids Extracted from Rainbow Trout (Oncorhynchus mykiss) Fed with Carotenoids and Conjugated Linoleic Acid

Eum, Gwang-Sik,Kim, Jeong-Gyun,Ha, Yeong-Lae,Park, Chan-II,Kang, Seok-Joong,Choi, Yeung-Joon,Choi, Byeong-Dae The Korean Society of Fisheries and Aquatic Scienc 2009 Fisheries and Aquatic Sciences Vol.12 No.4

A commercial diet supplemented with carotenoids and conjugated linoleic acid was fed to rainbow trout (Oncorhynchus mykiss) for 8 weeks. To investigate the anti-oxidative properties of these compounds, lipids from the muscle and viscera of the fish were subjected to different assays. At $10\;{\mu}g/mL$, L-ascorbic acid exhibited 95% 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, while the tissue lipids showed little radical scavenging activity. At 50 and $100\;{\mu}g/mL$, the lipids of the muscles and viscera showed 11.7-22.6% and 11.3-24.9% DPPH radical-scavenging activity, respectively. A lipid peroxidation inhibitory assay using the ferric thiocyanate method was also performed in comparison with $\alpha$-tocopherol at a concentration of 6.0 mg/mL. Our results indicate that the anti-oxidative property of the lipids in fish muscle, which was 85.2% compared to 85.3% for the visceral lipids, was stronger than that of $\alpha$-tocopherol (74.3%) following 3 days of storage at $40^{\circ}C$.

뱃멀미지수와 해군 함정 승조원의 멀미약 소비량과의 관계

김도연 ( Do Yeon Kim ),박기웅 ( Ki Woong Park ),양수경 ( Soo Kyung Yang ),신기식 ( Ki Sik Shin ),엄석현 ( Suk Hyun Eum ),한광남 ( Gwang Nam Han ),김명원 ( Myung Won Kim ) 국군의무사령부 2019 대한군진의학학술지 Vol.50 No.1

Objectives; Korea Hydrographic and Oceanographic Agency (KHOA) developed Korean motion sickness index which estimates the incidence of sea sickness and helps prevention of sea sickness for citizens and ship crew. Because avoidance of sea sickness is impossible for naval crew, prevention and supply of anti-sea medication is important in sailing. Method and material; This research was an ecologic study in two naval destroyers. The study included available sailing days which includes domestic sail records from April 2018 to October 2018. We collected daily Korean motion sickness index from KHOA and daily supply of anti-motion sickness medication. Motion sickness index was divided into 5 stages (Very good, good, usual, bad, very bad). We defined anti-motion sickness medication consumption rate (AMCR) as (total consumption of anti-motion sickness medication) / (total crew numbers) X 100. Motion sickness index and AMCR were compared with univariate analysis. Results; Among available 60 sailing days, motion sickness index of good was 15 days, usual was 31, and bad was 14 days. There was no very good or very bad days among sailing days. Average scores of components in motion sickness index were 3.32 for wave height, 4.42 for ship weight, 1.55 for sailing time, and 2.72 for maximum wind speed. Averagic AMCR were 2.44%. AMCR for good sailing day was 0.22%, usual was 1.36%, and bad was 6.99%. The medication consumption was increased in bad sailing day by 31 times. Conclusions; We found inverse correlation between motion sickness index and AMCR in this study. Before sailing, supply of motion sickness medication could be estimated through Korean motion sickness index.

인진쑥 노루궁뎅이 버섯균사체 발효물의 항산화 활성

김승섭(Seung-Sub Kim),경인구(Inn-Goo Kyeong),이미라(Mi-La Lee),김동구(Dong-Goo Kim),신지영(Ji-Young Shin),양진이(Jin-Yi Yang),이광호(Gwang-Ho Lee),음원식(Won-Sik Eum),강정훈(Jung-Hoon Kang) 한국유화학회 2014 한국응용과학기술학회지 Vol.31 No.4

The hot water extract from Artemisia capillaris fermented with Hericium erinaceum mycelium (AC-HE) were assessed for the protection against oxidative modification of biological macromolecules and cell death. Antioxidant activity of AC-HE evaluated using 2,2-diphenyl-1-picrylhydrazyl radical, 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical and peroxyl radical scavenging assays. AC-HE showed 61.73% DPPH radical scavenging activity at 500μg/mL, 97.39% ABTS radical scavenging activity at 250 μg/mL, and 44.18% peroxyl radical scavenging activity at 100 μg/mL. AC-HE were shown to significantly inhibited DNA strand breakage induced by peroxyl radical. AC-HE also prevented peroxyl radical-mediated human serum albumin modification. AC-HE effectively inhibited H2O2 induced cell death and significantly increased of the 11.47% cell survival at 100 μg/mL. AC-HE also decreased intracellular reactive oxygen species (ROS) levels in H2O2-treated cells. The results suggested that AC-HE can contribute to antioxidant and protected cells from oxidative stress-induced cell injury. 노루궁뎅이 버섯균사체(Hericium erinaceum)를 인진쑥(Artemisia capillaris)에 배양하여 조제한 AC-HE 열수 추출물이 생체고분자의 산화적 손상과 세포사멸을 보호할 수 있는 지를 관찰하였다. AC-HE의 항산화 활성을 DPPH radical, ABTS radical, peroxyl radical 소거활성 측정을 통해 알아보았다. 그 결과 DPPH radical 소거활성은 500 μg/mL 농도에서 61.73%, ABTS radical 소거활성은 250μg/mL 농도에서 97.39%, peroxyl radical 소거활성은 100 μg/mL 농도에서 44.18%로 나타났다. AC-HE은 DNA의 산화적 손상을 효과적으로 억제하였다. 또한 생체고분자물질인 사람의 혈청단백질의 산화적 손상을 억제하였다. 세포에 H2O2를 처리하였을 때 세포생존율에 비하여 발효물을 100 μg/mL농도로 전 처리한 세포생존율은 11.47% 증가했으며, 발효물을 50 μg/mL 농도로 처리했을 경우 세포내 ROS의 축적이 유의적으로 감소되었다. 따라서 AC-HE은 항산화 활성뿐만 아니라 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용이 뛰어난 것으로 사료되었다.

노루궁뎅이 버섯균사체를 이용한 비타민나무 발효물이 생체고분자의 산화적 변형과 세포사멸에 미치는 보호 영향

김승섭(Seung-Sub Kim),경인구(Inn-Goo Kyeong),이미라(Mi-La Lee),김동구(Dong-Goo Kim),신지영(Ji-Young Shin),양진이(Jin-Yi Yang),이광호(Gwang-Ho Lee),음원식(Won-Sik Eum),강정훈(Jung-Hoon Kang) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.1

본 연구에서는 노루궁뎅이 버섯균사체를 비타민나무 잎에 배양하여 조제한 노루궁뎅이 버섯균사체-비타민나무 발효물 열수 추출물이 생체고분자의 산화적 손상과 세포사멸을 보호할 수 있는지를 관찰하였다. 노루궁뎅이 버섯균사체-비타민나무 발효물의 항산화 활성을 DPPH radical, ABTS radical, peroxyl radical 소거활성 측정을 통해 알아보았다. 그 결과 노루궁뎅이 버섯균사체-비타민나무 발효물을 처리한 DPPH radical 소거활성은 500 μg/mL 농도에서 65.06%, ABTS radical 소거활성은 50 μg/mL 농도에서 98.83%, peroxyl radical 소거활성은 100 μg/mL 농도에서 44.03%로 높은 항산화 활성을 나타내었다. 노루궁뎅이 버섯균사체-비타민나무 발효물은 DNA의 산화적 손상을 효과적으로 억제하였다. 노루궁뎅이 버섯균사체-비타민나무 발효물 역시 사람의 혈청단백질과 Cu,Zn-SOD의 산화적 손상을 억제하였다. 세포에 H₂O₂를 처리하였을 때 세포생존율에 비하여 발효물을 100 μg/mL 농도로 전 처리한 세포생존율은 21.59% 높게 증가되었다. 또한 발효물을 50 μg/mL 농도로 처리했을 경우 세포 내 ROS의 축적이 유의적으로 감소되었다. 따라서 노루궁뎅이 버섯균사체-비타민나무 발효물은 항산화 활성뿐만 아니라 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용이 뛰어난 것으로 사료된다. In this study, hot water extract from sea buckthorn (Hippophae rhamnoides L.) leaves fermented with Hericium erinaceum mycelium (SBT-HE) was assessed for protection against oxidative modification of biological macromolecules and cell death. Antioxidant activity of SBT-HE was evaluated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2"-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, and peroxyl radical scavenging assays. SBT-HE showed 65.06% DPPH radical scavenging activity at 500 μg/mL, 98.83% ABTS radical scavenging activity at 50 μg/mL, and 44.03% peroxyl radical scavenging activity at 100 μg/mL. SBT-HE significantly inhibited DNA strand breakage induced by peroxyl radical. SBT-HE also prevented peroxyl radical-mediated human serum albumin modification. SBT-HE effectively inhibited H2O2-induced cell death and significantly increased cell survival by 21.59% at 100 μg/mL. SBT-HE also reduced intracellular reactive oxygen species levels in H₂O₂-treated cells. The results suggest that SBT-HE can contribute to antioxidant activity and protect cells from oxidative stress-induced cell injury.