Thermomicrobium roseum 유래 Alcohol Dehydrogenase의 정제 및 특성

윤석영,홍민표,김은호,공광훈 중앙대학교 기초과학연구소 2000 基礎科學硏究所 論文集 Vol.14 No.-

Alcohol dehydrogenase from Thermomicrobium roseum was purified to electrophoretic homogeneity approximately 77-fold with a 38% activity yield by adenosine 5'-monophosphate and Mono Q column chromatography. The molecular weight of the enzyme was determined to be approximately 43,000 by SDS-polyacrylamide gel electrophoresis and 88,000 by gel chromatography, indicating a homodimeric structure. The Km and Vmax values of the enzyme for ethanol were 24.2 mM and 12.8 ㎛ol/min/mg, respectively. Pyrazole notably inhibited the enzymatic activity. Ca2+ ion activated the enzyme activity, but Cu2+ inhibited. The activity of the enzyme was optimal at pH 10.0 and 60℃.

후두 및 하인두 편평세포암종의 경부곽청술에서 Level Ⅰ의 절제는 필요한가?

최은창,김영호,김동영,이세영,홍원표 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

톨루엔의 급성흡입이 마우스의 1차 체액성 면역반응에 미치는 영향

표명윤,주은영 숙명여자대학교 약학연구소 1998 약학논문집-숙명여자대학교 Vol.15 No.-

Toluene was inhaled at concentration of 10,000ppm to ICR mice for 10, 20, 30min, and mice were sacrificed on day 2, 4, 6 or 8 following inhalation. Acute toluene inhalation didn't change the body weight gains, thymus and kidney weights. Spleen weight was slightly decreased, but there's no significant difference. Liver weight was significantly decreased on the 6th day after inhalation and recovered on the 8th day after. The number of WBC was significantly decreased on the 4th day and recovery from toluene hazard was appeared on the 8th day. Platelets were markedly and significantly decreased only on the 2nd day in every inhaled group. Red blood cells were increased on the 6th day and recovered on the 8th day after. The number of IgM plaque forming cells in splenocytes and IgM level in antiserum were increased in every inhaled group when toluene( 10,000 ppm, 10, 20, 30 min) was exposed to mice prior to primary immunization. When mice were exposed to toluene after immunization, IgM plaque forming cells and IgM level were increased in 10 min inhaled group and similar to PFC of control in 30 min inhaled group. Therefore the acute toluene inhalation at high dose in mice seems to increase the primary humoral immune response irrespective to the time of SRBC-antigen injection.

Cisplatin이 1차 감작된 마우스의 IgM 용혈반 형성 세포수와 임파구 증식능에 미치는 영향

표명윤,강명옥,윤희은,주은영 숙명여자대학교 약학연구소 1996 약학논문집-숙명여자대학교 Vol.12 No.-

The effects of cisplatin on the IgM plaque forming cells and the lymphocyte proliferation in primarily sensitized mice were investigated. Cisplatin was i.p. injected with a single low does (0.825, 1.65, 3.3mg/kg, which is 5, 10, 20% of LD_50) to mice. The splenic IgM antibody plaque forming cell (PEC) was assayed after ICR mice had been treated in vivo with a combination of three different doses of Cisplatin at different time (day-2, day+2) plus SRBC(day 0). IgM PFC was significantly increased when drug was injected prior to immunization with SRBC, but it was decreased when administered after SRBC antigen. Splenocytes from mice injected with Cisplation(1.65, 3.3mg/kg) on the 2nd day before the test day were cultured with Con A and LPS. The splenocyte proliferation to Con A mitogen was slightly increased, but to LPS was significantly and markedly increased. It was found that Cisplatin at low dose showed either immunosuppression or immunostimulation, depending on the time of drug-application in relation to antigenic treatment.

톨루엔의 급성 흡입이 마우스의 자발운동량에 미치는 영향

표명윤,주은영 숙명여자대학교 환경과학연구센터 1996 환경과학논문집 Vol.4 No.-

To investigate the effects of acute toluene exposure on the spontaneous locomotor activity. Female ICR mice were exposed for varying periods of time(10, 20, 30min)to 10,000ppm toluene and were tested at intervals during and after exposure. The spontaneous locomotor activity was markedly de­creased at high dose(10,000ppm) toluene inhalation. Rapidity of recovery was dependent upon the time of inhalation.

병원정보시스템의 경영전략적 활용방안에 관한 연구

이은효,신종연,윤병준,김영훈 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

This study includes an empirical analysis of utilization of strategic management with hospital information system and a policy implication on hospital management. The purposes of this study are to analyze the effects of strategic information and implementation on the major performance factors. For this study. the materials used are mailing survey data of 65 hospitals and related literature was reviewed. Crosstabulation and multiple response analysis were made to find the frequencies by groups. ANOVA and multiple regression analysis were adopted to examine the differences and the relationship between variables involved. The major findings are as follow: 1. Internal information needed for strategic management focuses on the patients' data and the ones of services being delivered. Using portion of financial indices also ranked 2nd by respondents. but financial analysis was not performed frequently. 2. The Effects of strategic information on the hospital management environment were the most in hospital planning and hospital administration and medical support tasks. Ane the effects of assessment of the external factors and rapidity and accuracy of decision making, however. were relatively low. 3. The more computer system group satisfy planning group on the material requested by planning group. the more effects of cost reduction and service promotion have favorable results. Besides. the more computer education and PR are performed. the more efficiencies of hospital management have favorable results. In result of more exploration, the relationship of between computer education and PR item and satisfaction level of planning group on requested material and cost reduction effects was statistically significant. Of the above results. there are favorable effects on the major performance factors. However. there are few results of being performed on systematic strategic planning, action and management. With regard to hospital management information system, there are many challenges in the future when applying strategic management methods. Hospital executives must begin to use information system strategically for survival and growth in the 2000s.

부산시 고령자의 통행행태에 관한 연구

오윤표,안영종,김은정 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.29 No.1

The purpose of this study os to analyze the elderly's travel behavior and to measure the factors affecting their travel mode choice. Questionnaire survey was conducted for this study. It is assumed that the elderly is over 65 years old for this study. The major results of this study are as follows:(1) Because driving alone is very expensive travel mode, bus is considered as main travel mode for the elderly over 65 years old. (2) The friendship, recreation, an medical treatment are significant travel purpose. It was showed that the elderly's travel pattern is irregular. (3) Going up and down of stairs are the most important restriction for the elderly's travel.

의사소통의 활성화가 수학적 추론 능력의 발달에 미치는 영향에 관한 사례연구

김익표,권은영,최정현 大邱大學校 師範大學 附設 敎育硏究所 2010 學校敎育硏究 Vol.5 No.2

NCTM(2000, p.60)은 수학적 추론 능력의 신장을 위한 의사소통의 활성화를 강조하고 있다. 그리고 추론을 포함한 수학적인 활동의 활성화를 위한 학생들 사이의 의사소통을 강조한 사례연구(Elbers, 2003; Goos, 2004)도 소개되었다. 이와 같이 많은 문헌들이 수학적 추론과 의사소통과의 관련성을 언급 하고 있지만 서로 어느 정도의 영향을 미치고 있는지에 대한 연구가 부족한 실정이다. 추론과 의사소통 을 포함한 수학적인 활동들이 별개의 것이 아닌 통합된 전체의 각 부분을 차지하면서 밀접하게 관련되어 있음을 인지하는 것은 학교 수학에서 중요한 부분이다. 따라서 수학교육의 목표를 향하여 가는 학생 들에게 관계망으로 연결된 수학적인 활동들은 효과적으로 목표에 도달할 수 있도록 도와주는 뼈대의 역할을 할 것이다. 본 연구의 목적은 학교 수학에서 의사소통의 활성화가 수학적 추론능력의 신장에 미치는 영향의 분석이다. 이를 위하여 학생들에게 단계적인 과제의 제시를 통하여 의사소통의 활성화를 유도하고 이것이 수학적 추론 능력의 신장에 미치는 영향을 탐색하고자 한다. The importance of mathematical communication and mathematical reasoning is currently standing out in mathematics education. As a matter of fact, lots of theses and documents are dealing with this subject, and are mentioning that these two factors develop together. However, there are few qualitative case studies in this fields. So, this study aimed at qualitative case study about the effects of active communication on mathematical reasoning devolopment throughout the experiments. This study recorded the process of improving mathematical reasoning through mathematical communication. For this, various problems which are from basic to advanced were presented by degrees. We hope this study has a lot of discussions about various methods in order that mathematical communication occurs actively in schools and that students become to recognize the importance of mathematical reasoning.

결장직장암 환자의 임상적 고찰 : 조선대학교 부속병원의 10년 간의 결과

나은종,문성표,장정환,김권천,민영돈,김성환,조현진,김정용,김경종 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: The colorectal cancer rates 4th in the total cancer prevalence in Korea. The clinical patient demographics were considered with the incidence rate of the colon and rectal cancer. The clinicopathological factors were analyzed for the 5 year survival rate. Materials Methods: Of the 282 patients who were operated from Jan. 1, 1992 to Dec. 31, 2001 and diagnosed by pathological biopsy as colo-rectal cancer, 234 patients were followed up. The age, sex, location of the cancer, TNM stage, operation method, histopathological classification, metastatic region, complications after operation and the survival rate were investigated. Results: Of the 234 patients, male and female consisted of 120 cases (51.3%) and 114 cases (46.7%), with an average age of 61.6 years. The average follow up period was 52.3 months and the peri operative mortality was 5.6%. The site of the cancer was most frequent at the rectum with 127 cases (54.3%). The patients were classified according to the TNM stages with 38 cases of stage 1 (16.2%), 98 cases of II (41.9%), 72 cases of III (30.8%) and 26 cases of IV (11.1%). The 5 year survival rate in the age group older than 50 was 63.3 % and 73.5% in the group younger than 50. In regard to the operation type, the 5 year survival rate was 65.4% for elective operation and 65.8% for emergency operation. The 5 year survival rate for well differentiated, moderately differentiated, poorly differentiated cancer, and mucinous adenocarcinoma was 73.7%, 60.2%, 58.3%, and 92.6%, respectively. The 5 year survival rate of each of the TNM stage is 81.1%, 74.8%, 57.0%, and 11.5% for Classes I, II, III, and IV, respectively. Conclusion: The clinical factors were analyzed for the 5 year survival rate with no statistical significance for the age, sex, and location of the cancer. The TNM stage and histopathological differentiation were statistically significant. The colon cancer showed better prognosis compared to the rectal cancer.

Calcium Carbonate와 fibrin adhesive의 병용이 성견 2급 치근분지부 치주조직 재생에 미치는 영향

서은표,정현주,김영준 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The purpose of this study was to evaluate the effect of combined use of fibrin tissue adhesive and porous resorbable calcium carbonate on the periodontal regeneration of the class Ⅱ furcation defects in dogs. Class Ⅱ furcation defect was surgically created on the second, third, and fourth premolars bilaterally in the mandibles of six mongrel dogs. The experimental sites were divided into four groups according to the treatment modalities: Control-surgical debridement only; GroupⅠ-calcium carbonate grafting; Group Ⅱ-application of fibrin adhesive only; Group Ⅲ-application of fibrin adhesive after calcium carbonate grafting. The animals were sacrificed at 2, 4, and 12 weeks after periodontal surgery and the decalcified specimens were prepared for histologic and histometric examination. The results are as follows : Clinically, there were no inflammatory response in all groups after 2, 4, and 12 weeks. In the Control group, junctional epithelium migrated apically to the reference notch. In Group Ⅰ, graft materials exfoliated from the defect throughout the experimental periods and new bone was seen in the notch area at 4 and 12 weeks' specimens. At 2 weeks, fibrin adhesive was indistinguishable in Group Ⅱ. In Group Ⅲ, the graft material was maintained in the defect throughout the experimental periods and the amount of periodontal tissue regeneration was greater than other groups. These results suggest that the use of fibrin tissue adhesive in conjunction with porous resorbable calcium carbonate would improve the stability of graft material and inhibit the epithelial downgrowth and contribute to periodontal regeneration.