분자역학적 방법을 이용한 부산시내 대형건물 냉각탑수의 레지오넬라균의 형별 분류

김정만,정석훈,서대영,박은희,송은주,최재철,이은엽,장철훈 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

결핵균 약제감수성 검사의 비용효율성에 관한 다기관 연구

정석훈,이대동,최재철,김선주,신정환,정윤성,이은엽,오승환,배길한,장철훈 대한감염학회 2005 감염과 화학요법 Vol.37 No.1

목적 : 우리나라에서의 결핵균 감수성 검사는 검출 균주의 일부에서만 시행되고 있다. 본 연구에서는 모든 검출 균주들에 대해서 감수성검사를 실시하면서, 진료에 필요한 결과를 충분히 제공하고 경제적이면서 현실적인 감수성검사 방안을 제시하고자 한다. 재료 및 방법 : 5개 대학병원에서 일정기간 연속적으로 의뢰된 502균주의 감수성 결과를 분석하였다. 검사결과의 해석 및 2차 약제 감수성검사의 필요성은 NCCLS approved standard M24-A의 권고 기준에 따라 판단하였다. 결과 : 최소한 1가지 이상의 약제에 내성을 보인 경우는 초치료 환자의 10% (38/363), 재치료 환자의 61%(85/139)였으며, 다약제 내성을 보인 경우는 초치료 환자의 3% (11/363), 재치료 환자의 44% (61/139)였다. NCCLS 권고에 따라 2차 약제에 대한 감수성 검사를 시행하지 않아도 되는 경우는 초치료 환자의 96%, 재치료환자의 47%였다. 결론 : 초치료 환자는 95%에서 1차 약제에 대한 검사만으로 충분하므로 필요한 경우에만 2차 약제를 추가로 검사하고 재치료 환자에서는 1, 2차 약제를 동시에 검사할 필요가 있을 것으로 생각한다. Background : The anti-mycobacterial susceptibility test is performed on only a small percentage of clinical isolates in Korea. The aim of this study is to propose an anti-mycobacterial susceptibility testing scheme, which is not only economic and practical but also fully informative to physicians. Materials and Methods : The anti-mycobacterial susceptibility test results of 502 strains, isolated from five university-affiliated hospitals, were analysed. The interpretation of the results and the need for second-line drug susceptibility test were judged according to the recommendation of NCCLS M24-A guidelines. Results : The isolates from 10% (38/363) of treatment-navie patients and 61% (85/139) of retreatment patients showed resistance to at least one of the anti-mycobactial agents; 3% (11/363) and 44% (61/139) of isolates from each group were multi-drug resistant. According to the recommendation by NCCLS, the percentage of patients not needing the susceptibility test results for second-line drugs were 96% for treatment-naive and 47% for re-treatment patients. Conclusion : Since the susceptibility test against first-line drug is sufficient for 95% of treatment-navie patients with tuberculosis patients, susceptibility test against second-line drugs may be performed only when it is necessary. As for the re-treatment patients with tuberculosis, susceptibility test for both first-line and second-line drugs should be performed simultaneously.

신경회로망을 이용한 이면비드 폭 예측에 관한 연구

정재원,김일수,박창언,김학형,서주환,김인주,심지연 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

The development of robotic welding process is a very complex assignment because the system is affected by a number of process parameters which are very difficult to determine or predict in practice. The full automation welding has not yet been achieved partly because the mathematical model for the process parameters of a given welding task is not fully understood and quantified. Several mathematical models to control welding quality, Productivity, micro structure and weld properties in arc welding processed have been studied. However, it is not an easy task to apply them to the various practical situations because the relationship between the process parameters and the bead geometry is non-linear and also they are usually dependent on specific experimental result. Practically, it is difficult, but important to know how to establish a mathematical model that can predict the result of the actual welding process and how to select the optimum welding condition under a certain constraint. In this paper, an attempt has been made to develop an neural network model to predict the back-bead width as a function of key process parameters in the GMA (Gas Metal Arc) welding and to compare the developed models with the experimental results

석이버섯 용매 추출물의 항산화 및 아질산염 소거작용

정은재 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

본 연구는 석이버섯의 기능성을 검토하기 위하여 석이버섯을 각종 용매로 추출하여 이들 추출물의 전자공여 작용, 항산화 효과 및 아질산염 소거작용을 측정하였다. 석이버섯 diethylether 및 butanol 추출물의 폐놀성 화합물의 함량은 각각 194.20㎍/ml, 98.80㎍/ml으로 높게 나타났다. 그러나 petroleum ether와 hexane 추출물에서는 페놀성 화합물이 거의 존재하지 않았다. 석이버섯 diethylether 및 butanol 추출물의 진자공여 작용은 각각 80.79%, 72.81%로서 높게 나타났으며, DPPH와의 반응초기에 흡광도가 급격히 감소하였다. 석이버섯 diethylether 및 butanol 추출물의 항상화 효과는 대조군보다 우수하였으나, BHA보다는 모두 낮은 항산화 효과를 나타내었다. 석이버섯의 아질산염 소거작용은 diethylether 및 butanol 추출물에서 각각 96.07%, 72.39%으로 나타내었다. 그러나 페놀성 화합물이 거의 없는 petroleum ether와 hexane 추출물에서는 아질산염 소거작용이 각각 15.07%, 7.3%로서 약하게 나타났다. This study was conducted to investigate electron donating ability, antioxidative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, hutanol, petroleum ether and hexane extracts were 194.20㎍/ml, 98.80㎍/ml, 1.60㎍/ml and 0.2㎍/ml, respectively. Electron donating abilities of diethylether and butanol extracts from Gyrophora esculenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethylether and butanol extracts sharply increased at initial stage. Antioxidative activities of diethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low as compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum etherextracts from Gyropyhora esculenta were 96.07%, 77.39% and 15.07%, respectively.

컴퓨터 시뮬레이션을 통한 치어사육용 다단계 배양시스템의 개발 가능성 탐색

정은수,조덕제,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

It was researched that the possibility for developing multistage culture system to cultivate larvae by computer simulation with basic experiments. This culture system was composed of 3 stages. Chlorella sp. at the 1st stage are being supplied to the rotifer(Brachionus plicatilis) at 2nd stage, and the rotifer are bing feeded to larvae at the 3rd stage. In this study, Chlorella sp. were cultivated by batch culture to search for the possibility of continuous feeding rate Chlorella sp., ???????? and ??(cells/hㆍrotifer) in the multistage culture system, then the change of the rotifer concentration at 2nd stage was simulated by computer. The required amount of rotifer for the growth of larvae was also increased as the increase of the length of larvae. On the 9th day of the culture, the rotifer uptake rate of larvae was 250(cells/dayㆍlarvae). Based on these basic experiments and results. It was suggested that the possibility of multistage culture system to cultivate larvae with continuous feeding of Chlorella sp. and rotifer.

靑皮가 마우스의 免疫細胞에 미치는 影響

殷載淳,吳贊鎬,鄭鉉雨 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

靑皮는 疏肝破氣 및 消積化滯하는 작용이 있는 약물로 대부분 氣滯로 인한 증상들에 사용되고 있지만 임상상 活血之劑와 配合하여 氣滯血瘀나 血瘀脇痛등에 應用되고 있다. 氣滯는 각종의 스트레스등으로 인하여 발생되어 血行을 不暢하게 하고, 심지어는 正氣까지도 손상시킨다. 正氣의 손상은 바로 免疫機能의 低下라고도 말할 수 있는데, 免疫이란 非自己로 인식된 異常細胞를 처리함으로써 個體의 恒常性을 維持하려는 현상을 말한다. 면역에 先天的 免疫과 T림프구 및 B림프구가 관여하는 特異性 免疫이 있고, 免疫作用에 관여하는 細胞로는 特異性 免疫에 관여하는 免疫細胞外에도 Macrophage, NK cell, 수지상 세포 및 랑게르한스 세포등을 들 수 있다. 뇌의 기능은 심장에서 방출되는 혈액의 공급에 의해 이루어지는데, 만약 혈액의 공급이 제대로 이루어지지 않으면 많은 질환들이 발생한다. 그 중 虛血性 腦疾患은 충분한 양의 혈액이 뇌를 공급되지 않거나 공급되고 있는 혈액내의 산소농도가 떨어져 발생하는 것으로써 임상적으로는 運動麻痺·知覺痲痺등 神經機能 消失등의 증상을 보이기 때문에 東醫學的으로는 이를 風病이라 하고, 風病은 대체적으로 陰虛나 血虛등으로도 발생할 수 있어 陰虛動風證 또는 血虛生風證등의 病證들이 있다. 그리하여 각종 氣滯로 인한 氣滯則血滯가 발생되거나 또는 正氣가 손상되어 氣虛則血虛하기 때문에 陰虛나 血虛등으로 인한 運動麻痺나 知覺麻痺가 발생한다. 이러한 이유등으로 本 硏究者들은 혈류장애 질환치료에 사용되는 韓藥材中 靑皮의 작용을 규명하는 硏究의 일환으로 靑皮가 면역기능에 어떠한 영향을 미치는가에 대하여 알아보기 위하여 동결건조된 靑皮를 마우스에 1일 1회씩 7일간 투여한 후 T-lymphcyte의 Apoptosis와 sub-population, 그리고 proliferation 및 Peritoneal macrophage의 nitric oxide 생성과 phagocytic activity를 관찰한 결과 DNA fragmentation과 sub-population에는 별다른 영향을 주지 않았지만 proliferation에 있어서는 증식이 촉진되었고, 복강 macrophage의 nitric oxide 생성과 phagocytic activity에 있어서는 NO는 감소하였으나 phagocytic activity는 현저히 증가하였다. 이는 靑皮가 NO 생성을 억제하여 phagocytic activity를 증가시킨 결과라 思料된다. 이상과 같은 實驗結果들을 통해서 볼 때 靑皮는 臨床的으로 각종 氣滯로 인한 正氣損傷과 血液循環障碍가 동시에 나타날 때에만 正氣를 보충해주는 役割을 할 수 있을 것으로 사료된다. The purpose of this research was to investigate effects of Citri Reticulatae Viride Pericarpium water extract(CRVP) on immunocytes in mice. The administration of CRVP did not affect apoptosis and sub-population of mouse T-lymphocytes, but increased the proliferation of T-lymphocytes. CRVP decreased nitric oxide production from mouse peritoneal macrophage, but increased a phagocytic activity of macrophages. These results suggest that CRVP does not affect T cell mediated immunity, but increases the phagocytic activity of peritoneal macrophages.

흰쥐에서 심정지후 Pentoxifylline이 뇌의 허혈-재관류 손상에 미치는 효과

정시경,김영민,오동렬,최경호,박승현,이운정,박규남,유은영,이원재,김세경 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Two major events occurring in the cerebral hemodynamics after successful resuscitation from cardiac arrest are reactive hyperemia and postischemic hypoperfusion. In this study, we examined the effect of Pentoxifylline(PTX) on the rat brain following cardiac arrest. Methods: Fourteen rats were anesthetized and artificially ventilated. Cardiac arrest was produced by chest compression and clamping of tracheal tube for 3 minutes in ketamine anesthetized rats. Circulation was restored by standard cardiopulmonary resuscitation methods. In 7 rats, PTX 10mg/kg was infused at 10min after cardiac arrest(PTX group). In the other 7 rats, same amount of normal saline was infused(control group). Results: In both groups, hemodynamic variables, neurologic deficit(ND) score and histopathologic findings of hippocampal CA1 neurons were observed. Hemodynamic variables and ND score were not significantly different between two groups. Delayed ischemic neurons of hippocampal CA1 were decreased in PTX group(2.2±2.4%) compared with control group(9.1±1.2%). Conclusions: We conclude that PTX prevented development of delayed ischemic neurons in hippocampal CA1 after cardiac arrest. PTX may be useful in emergency situations following cardiac arrest.

경남지역 건강검진자에서의 혈청지질에 관한 연구

정준훈,박현모,류재오,조동자,표미순,오은경 한국건강관리협회 2000 건협 Report Vol.3 No.-

실시간 처리를 위한 복합 광 패턴 인식 시스템에 관한 연구

은재정 國立 昌原大學校 産業技術硏究所 1990 産技硏論文集 Vol.4 No.-

An optical pattern recognition system using spatial light modulator (SLM) and CGH is implemented, and correlation characteristics of Matched Spatial Filter(MSF) for given inputs are analyzed. The proposed system is composed of digital processor and optical processor. Filter bank and signal detector are the main elements for digital processing and LCD and CGH for optical processing. Modified version of LCD is used for real-time input device of optical correlator, and CGH-based fSDF(filter synthetic discriminant function) is fabricated on film mask for spatial matched filter in order to classify distorted images. Total system size is effectively reduced to 148.8㎝ by using lens combinations. Input patterns are obtained from the out-or -plane aircraft image by gradually rotating it 20 degrees, and then are used for SDF training images. After analyzing the characteristics of POF and BPOF having high light throughput and higher SNR comparted with conventional MSF the fSDF synthesizing algorithm including modulation procedures of thesse filters is proposed. In this paper, optical pattern recognition system adopting the advantages of high speed and parallelism of optical processing is implemented using real-time input device and CGH.

括數根 水溶性 蛋白質의 細胞毒性

殷載淳,鄭甲基,梁在憲,權鎭,吳贊鎬,全焄 又石大學校 1995 論文集 Vol.17 No.-

The purpose of this research was to investigate effect of water-soluble protein of Trichosanthes kirillowii(TKP) on the proliferation of human tumor cells. TKP inhibited the proliferation of HeLa cells and KHOS-NP cells. The inhibitory activity of doxorubicin on HeLa cells and its of mitomycin C on KHOS-NP cells were increased by the combination of TKP. TKP did not affect on the proliferation of Balb/c 3T3 cells, but inhibited the proliferation of mouse spleen cells and human lymphocyte at 1 ㎍/ml. These results suggest that TKP bas the cytotoxicity on HeLa and KHOS-NP cells, and increase the cytotoxicity of doxorubicin or mitomycin C.