Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

자궁경부암에서 Glutathione S Transferase-π의 분포에 관한 연구

양승하,김의한,강영근,김대중,강상균 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Uterine cervical carcinoma is the most common malignant tumor of woman in korea. GST-π is multifunctional and is implicated to detoxication of drug and carcinogenesis. This enzyme is expressed in many tumors of human. Relationship between GST-π and drug resistence on uterine cervical carcinoma is not known, but condyloma accuminatum, CIN and invasive carcinoma express this isoenzyme, and it is regarded as a useful marker in preneoplasia and neoplasia. To observe the expression of GST-π in CIN and invasive carcinoma, we analyzed CIN 26 cases and invasive squamous cell carcinoma 61 cases by immunohistochemical stain using monoclonal mouse anti-human GST-π. The results are as followings: 1) The GST-π showed positive reactionin normal and metaplastic epthelium adjacent to CIN and invasive carcinoma in forty-three cases(62.3%) of sixty-nine cases. 2) The GST-π showed positive reactionin CIN in eighteen cases(69.2%) of twenty-six cases. Positive rates of CIN Ⅰ, CIN Ⅱ and CIN Ⅲ are 66.7%, 71.5%, 68.8%, respectively. 3) The GST-π showed positive reaction in invasive squamous cell carcinoma in fifty-one cases(83.6%) of sixty-one cases. Positive rates of microinvasive and invasive squamous cell carcinoma are 84.6% and 83.3%, respectively. According to cell type, keratinizing and nonkeratinizing type are 81.8% and 84.6%, respectively. Above results revealed that GST-π may be significance as a immuohistochemical marker in CIN and invasive SCC, and may be implicated in intrinsic resistance for anticancer drug.

F형 종골 금속판의 역학적 특성

양규현,박영희,원정훈,김대야 대한골절학회 2004 대한골절학회지 Vol.17 No.2

심인성 다음다갈증 2예

양창국,최병무,한홍무,김영대,주승재 동아대학교의과대학 1992 동아의대학술지 Vol.4 No.1

만성신부전증 환자의 HLA 동종항체 분석

김대원,양윤선,김선희,오하영 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.1

침식된 유치 법랑질에 대한 레이저 조사 및 불소도포의 재광화 효과

양영숙,이광희,,김대업,라지영 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

연구목적은 산성 음료수에 의해 탈회된 법랑질의 표면에 대한 Nd:YAG 레이저 조사의 재광화 효과와 불화물 도표의 효과를 평가하는 것이다. 치아우식증, 색소, 손상, 부착물이 없는 건전한 사람의 유치의 평활면으로부터 법랑질 시편을 제작하고, 코카콜라에 12시간 동안 담가 탈회시켰다. 탈회된 법랑질 표면에 6 W 출력, 50 mJ, 20 Hz pulse rate 의 에너지 밀도로 Nd:YAG 레이저를 조사하였다. 탈회 전후와 레이저 조사 후에 미세경도 및 Diagnodent 점수를 측정한 후, 0.05% 불소 용액, 1.23% 불소 젤, 0.1% 불소 바니쉬 중의 한 가지를 도포하였으며 미세경도 및 Diagnodent 측정을 통하여 레이저의 재광화 효과와 불소의 효과를 평가하였다. 1. Diagnodent 측정치의 변화에서, 탈회 후에는 초기 점수의 23.51%로 감소하였고, 레이저 조사 후에는 초기 점수의 37.37%로 증가하였으며, 불소도포 후에는 초기 점수의 51.34%로 증가하였고, 전체적으로 보면 초기 점수, 탈회 후 점수, 레이저 조사 후의 점수, 불소도포 후의 점수 및 그 백분율 사이에 모두 유의한 차이가 있었다(p<0.05). 3개 불소군 사이의 차이는 초기점수 및 불소도포 후 점수와 백분율에서 모두 유의하지 않았으며, 각 불소군 내에서는 불소 용액군과 불소 바니쉬군에서 초기 점수, 탈회후 점수, 레이저 조사 후의 점수, 불소도포 후의 점수 및 그 백분율사이에 모두 유의한 차이가 있었고, 불소 젤군에서는 레이저 조사 후와 불소도포 후의 점수 및 백분율 사이에 유의한 차이가 없었다. 2. 미세경도의 변화에서, 탈회 후의 경도는 초기 경도의 33.58%로 감소하였고, 레이저 조사 후의 경도는 초기 경도의 43.99%로 증가하였으며, 불소도포 후의 경도는 초기 경도의 51.38%로 증가하였고, 전체적으로 보면 초기 경도, 탈회후 경도, 레이저 조사후의 경도, 불소도포 후의 경도 및 그 백분율 사이에 모두 유의한 차이가 있었다(P<0.05). 불소군 별로 보면 3개 불소군 사이의 차이는 모두 유의하지 않았고, 백분율에서도 초기경도와 불소도포 후 경도에서 유의하지 않았으며, 각 불소군 내에서는 초기 경도, 탈회 후 경도, 레이저 조사 후의 경도, 불소도포 후의 경도 및 백분율 사이에 모두 유의한 차이가 있었다. The purpose of this in vitro study was to evaluate the remineralizing effects of Nd:YAG laser irradiation and fluorides application on primary tooth enamel eroded by acidic drink. The materials were 30 sound primary teeth with intact smooth enamel surfaces. They were demineralized with Coca-cola at 37℃ for 12 hours and then irradiated by Nd:YAG laser with 6W power, 50mJ/㎠ energy density, and 20 Hz pulse repetition. After laser irradiation. teeth were treated by three kinds of fluorides: (1) 0.05% NaF solution, (2) 1.23% APF gel and (3) 0.1% fluoride varnish. Diagnodent scores and microhardness (VHN) were measured before and after the each treatment. The results were as follows: 1. Diagnodent scores decreased to 23.51% from the initial after demineralization, and then increased to 37.37% after laser irradiation, and to 51.34% after fluoride treatment. There were significant differences between the total scores of initial, demineralization, laser irradiation and fluoride treatment(P<0.05). There was no significant difference between scores after fluoride treatment according to fluoride types. 2. Microhardness(VHN) decreased to 33.58% from the initial after demineralization, and then increased to 43.99% after laser irradiation, and to 51.38% after fluoride treatment. There were significant differences between the total scores of initial, demineralization, laser irradiation and fluoride treatment (P<0.05). There was no significant difference between scores after fluoride treatment according to fluoride types.

퇴행성 요추부 질환에서 골이식 방법에 따른 치료 결과 : 후외방 유합술 및 후방 요추체간 유합술의 비교 Comparison between Posterolateral Fusion and Posterior Lumbar Interbody Fusion

양준영,이준규,김영모,남대철,강찬 대한정형외과학회 골.연부조직이식학회 2003 대한골.연부 조직이식학회지 Vol.3 No.1

생쥐 미세아교세포(BV2)에서 Corticotropin-releasing Hormone(CRH)에 의한 Nitric Oxide(NO)생성의 증가

양율희,양영,조대호 Official Journal of the Korean society for immunol 2004 Immune Network Vol.4 No.1

Adriamycin이 백서 심근에 미치는 영향에 관한 병리조직학적 연구

양종호,강대영 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

Adriamycin is one of the antracycline antibiotics used as a anticancer drug. It has diverse antineoplastic actions on tumor and commonly interacts with DNA. The clinical use of adriamycin is limited by various toxic side-effects, such as cardiotoxicity, which can lead to congestive heart failure. To study cardiotoxic effects of single high dose of adriamycin, a model of animal experiment was performed in the adult rats. Adriamycin was dissolved in isotonic saline(2mg/ml) and was administrated with 10mg per kilogram of body weight by a single injection via right jugular vein(B group) and an intraperitoneal injection (A group), after being anesthetized by ether. The rats were sacrificed at 6th hour, 1st, 2nd, 3rd, 4th, 5th, 6th, 7th, 14th, and 30th day, respectively. Light microscopic examinations were done at left ventricular myocardium. The results were as follows: 1. The myocardial lesions showed vacuolation of cardiac myocytes, loss of cross striations, homogenization of the sarcoplasm, interstitial edema, noninflammatory focal myocytolysis accompanied by interstitial fibroblastic infiltrations and myofiber atrophy. These lesions did not occur synchronously in all affected myocytes, and exhibited different evolutionary stages. Above mentioned findings were especially evident in the 7th experimental day group. In the 6th hour experimental group, the dilation of the extracellular spaces was noted. 2. The morphologic changes became mild in degree, following 14th experimental pay. 3. The morphologic changes were relatively evident in the intravenonusly injected group(B), but were not distinct in the intraperitoneally injected group(A). In summary, the results obtained by present study indicate that morphologic changes due to acute toxic effect become progressively disappeared after the restriction of adriamycin injection.

유세포 측정을 통한 CD34^(+)세포의 정량방법 비교

양형모,서영훈,엄현석,민창기,김희제,정대철,이종욱,진종율,한치화,김춘추,김학주 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.2

본 연구소에서는 현재 CD34^(+) 세포의 정량에 사용되고 있는 4가지 서로 다른 방법을 가지고 골수에 적용하여 그 결과를 비교하였다. 각각의 방법들이 편리한 점과 불리한 점들을 가지고 있었지만, 통계적으로는 유의한 차이를 보이지 않았다. 1) CD34-PE/CD45-FITC 방법13(ISHAGE: International Society of Hematotherapy and Graft Engineering), 2) CD34-PE/CD14-FITC 방법, 3) CD34-PE/CD14-FITC/CD45-PerCP 방법, 4) DNA dye/CD34-PE/CD45-PerCP4(Becton Dickinson) 등을 비교하였으며 그 결과 CD34^(+) 세포의 농도는 다음과 같았다. 1) 0.473±0.345, (%Mean±SD, N=70) 2) 0.663±0.519, (N=20), 3) 0.543±0.381 (N=46), 4) 0.624±0.341, (N=32). 시료로는 여의도 성모병원 카톨릭 조혈모세포이식 센터에서 채혈한 골수를 이용하였다. Four different methods for flow cytometric quantitation of CD34+ cell were compared using bone marrow samples (n=168). Following combination of antibodies were used for the analysis : A) CD45-FITC/CD34-PE (n=70), B) CD14-FITC/CD34-PE (n=20), C) CD14-FITC /CD34-PE /CD45-PerCP(n=46), D) DNA dye/CD34-PE/CD45-PerCP (n=32). Each method showed no significant differences among them (P=0.126, ANOVA test, SPSS Version 7.5). When we compare these methods using same samples (n=12), the P value became 0.832. The mean % of CD34+ cells calculated in this experiment were A) 0.474%, B) 0.663%, C) 0.548%, D) 0.624% respectively. In our laboratory, we decided to use CD45-FITC/CD34-PE methods as a routine protocol.