Disruption of rsmA Gene of Pectobacterium carotovorum subsp. carotovorum LY34 and Effect on Pathogenicity

Kim, Min Keun,Kang, Tae Ho,Kim, Sung Kyum,Jeong, Yu Seok,Yun, Han Dae,Kim, Hoon The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6

The rsmA gene was cloned from soft-rot bacterium Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34), and its role in pathogenicity was investigated by marker exchange mutagenesis. From a cosmid library of Pcc LY34 genomic DNA, a positive clone carrying the rsmA gene was selected, and the gene was cloned by polymerase chain reaction (PCR) amplification. The gene is 186 bp in size and encodes a protein of 62 amino acids with a predicted molecular mass of 6,839 Da. The calculated pI of the RsmA is 8.16. The phylogenetic tree showed that the RsmA of Pcc LY34 appeared genetically identical to the CsrA of Pectobacterium atrosepticum SCRI1043 (100% identity) and similar to the CsrA of Yersinia pestis KIM10+(98.3%). The gene was disrupted by the $Km^r$ gene, and the cells became mutated (i.e., $RsmA^-$ mutant). The pathogenicity test revealed that the disease rating of the $RsmA^-$ mutant only differed slightly from that of the wild type on a slice of potato tuber and a Chinese cabbage stalk. These results suggest that RsmA is not an essential factor for the pathogenicity of Pcc LY34 and that the rsmA gene of Pcc LY34 is not completely derepressed in the $RsmA^-$ mutant for virulence-related genes, contrary to the results of Erwinia carotovora subsp. carotovora $RsmA^-$ mutant, which proved hypervirulent for celery petioles. These results showed that the microenvironmental conditions of the host and/or strain of pathogen are important for the coordination of virulence gene expression.

Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells

Dahae Lee,Ki Sung Kang,Jae Sik Yu,Jung-Yoon Woo,Gwi Seo Hwang,Dae-Woon Eom,Seung-Hoon Baek,Hye Lim Lee,Ki Hyun Kim,Noriko Yamabe 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3

Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated. Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay. Results: The reduction in LLC-PK1 cell viability by 60mM FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with 60mM FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by 60mM FK506 treatment, whereas it was decreased after cotreatment with KRG. Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.

제 1형 신경섬유종증 환자에서 발생한 위장관 간질종양 1예

원경준,이준,변유미,조민근,한경택,김원,홍란,임성철,김경종,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract, but this tumor is rarely seen in association with type l neurofibromatosis (NFl) Generally, the majority of GISTs are isolated neoplasm and they are sporadically found in the stomach, but the GISTs in NFl patients are usually multiple and usually found in the small intestine. We report a case of multiple GISTs in the jejunum of a 63-year-old woman diagnosed as NFl accompanied by complication of gastrointestinal bleeding, In this case, Patient had multiple cafe-au lait spots and neurofibroma on skin and had freckling on axilla and groin, and then, we made a diagnosis of NFl. Gastrointestinal bleeding is controlled by resection of multiple GISTs. Generally, only 3-5% of all gastrointestinal bleeding comes from the small bowel. Causes of small intestinal bleeding are angiodysplasia, neoplasm, NSAIDs induced ulcer etc. but, If patients have NFl accompanied by complication of gastrointestinal bleeding, GISTs on small bowel must be considered.

Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells

Lee, Dahae,Kang, Ki Sung,Yu, Jae Sik,Woo, Jung-Yoon,Hwang, Gwi Seo,Eom, Dae-Woon,Baek, Seung-Hoon,Lee, Hye Lim,Kim, Ki Hyun,Yamabe, Noriko The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3

Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated. Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay. Results: The reduction in LLC-PK1 cell viability by $60{\mu}M$ FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with $60{\mu}M$ FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by $60{\mu}M$ FK506 treatment, whereas it was decreased after cotreatment with KRG. Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.

2019년도 2학기 신라대학교 재학생의 부산인식 설문조사 분석 : 인문사회계 학생을 대상으로

김대래, 신군재, 유영명, 장희정, 정쾌영 신라대학교 부산학연구센터 2016 부산연구 Vol.17 No.2

이 논문은 신라대학교에 재학 중인 학생들을 대상으로 부산에 대한 인식을 알아보기 위해 수행 한 설문지에 대한 분정확히 알아보기 위하여 분석에서는 부산출신자를 대상으로 하였다. 학생들과 부모의 출생연도와 연령 등에 관한 기초분석을 하고 부산의 자랑과 특성에 관한 인식을 분석 하였다. 아울러 학생들의 일석이다. 총 237부의 응답지를 받았는데 그 중에서 부산출생자 153명 을 대상으로 분석을 하였다. 부산에 관한 인식을 좀 더 상생활과 관련된 설문을 분석하였다. 분석에는 spss24를 이용하여 빈도분석과 기술통계분석 그리고 교차분석을 하였다.

불화석 용액내 불소농도의 장기변화와 첨가물의 영향에 관한 실험적 연구

유두선,이광희,최윤주,김대업 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2

The purpose of this study were to measure the solubility of the stannous fluoride experimentally,to find a method for improving the solubility of the stannous fluoride,and to observe the effect of longterm storage of the variation of the concentration of fluoride in the stannous fluoride solutions.By adding such materials as antiseptics,dye,flavor,and tests to solution,the variation of the fluoride concentration was also observed. Ten groups of 0.4% stannous fluoride solutions to which glycerine,sodium chloride,chlorhexidine,dye,flavor,xylitol,and sorbitol were added were prepared.The measurements were carried out by direct calibration. The obtained results were as follows. 1.Effect of adding glycerine as solvent:This solubility of the stannous fluoride increased in the case of adding glycerine.By increasing the glycerin concentration,the fluoride level in stannous fluoride solution also increased. 2.Effect of adding sodium chloride and chlorhexidine.:Comparing to the case of pure water,low fluoride level was measured in case of adding sodium chlorid and high fluoride level was measured in case of adding chlorhexidine. 3.Effect of adding erythrosin as dye and banna essence as flavor.:Adding erythrosin and banna essence didn't affect fluoride level. 4.Effect of adding xylitol and sorbitol.:The effects of xylitol and sorbitol were nearly the same as the effect of adding erythrosin and banna essence.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

ATM망에서 위반 셀을 이용한 트래픽제어에 관한 연구

김대영,김장형,이유경 濟州大學校 産業技術硏究所 1998 산업기술연구소논문집 Vol.9 No.1

In this paper, we proposed priority scheduling and buffer assignment algorithm using two finite buffers and multiple threshold values as a priority cell scheduling mechanism to serve efficiently multimedia traffic in ATM networks. Multimedia traffics can be categorized into four classes based on their real time properties and loss tolerance. we analysed priority control mechanisms to achieve the cell loss rate requirements and the delay requirements of each service class using an ATM switch with output buffer. Basic concept of proposed priority control scheme is flexible to serve the four classes service using double buffers in ATM Adaptation Layer( AAL) The service classes of our concern are satisfied with the CBR(Constant Bit Rate) class and VBR(Variable Bit Rate) class. The CBR is applied to Push Out cell loss priority method and the VBR is applied PBS to method with two thresholds. The performance of the proposed method is to firstly discard the tagged cell from the UPC parameter using two threshold is better than the VBR with one threshold.

2019년 2학기 ‘부산의 하루’ 수강생의 부산인식 설문조사 분석

김대래, 유영명, 정용석 신라대학교 부산학연구센터 2019 부산연구 Vol.17 No.2

이 논문은 2019년도 2학기 신라대학교에 재학 중인 학생들을 대상으로 부산에 대한 인식을 알 아보기 위해 수행한 설문지에 대한 분석이다. 총 115부의 응답지를 받았지만 부산출생자 69명을 대상으로 분석을 하였다. 이 조사는 매년 2학기에 이루어지고 그 분석결과는 부산연구에 게재되고 있다. 이와 같은 연구는 오랜 기간에 걸쳐 누적될 때 그 의미가 더 커지리라고 생각된다.

2014년도 신라대학교 '부산의 하루' 수강생 설문조사 결과 분석

김대래, 유명길 신라대학교 부산학연구센터 2017 부산연구 Vol.15 No.1

2014년도 '부산의 하루'를 수강하는 신라대학교 재학생 가운데 부산에서 출생한 182명을 대상으로 별첨의 설문조사를 한 결과 주요 내용은 다음과 같았다. 첫째, 인문사회계, 1학년, 남학생이 이 과목을 주로 수강하고 있엇으며, 수강신청 학생의 3/4의 학생들이 사전에 부산에 관해 교육을 받은적이 없었다. 둘째, 부산의 상징에 대해서는 해운대>광안대교>서면>벡스코>남포동 순서로 응답을 하였는데, 해운대와 광안대교가 압도적 선택을 받았다. 외지인들에게 구경시켜 주고 싶은 곳은 광안대교야경>해운대>서면>불꽃놀이>국제영화제 순으로 나타났는데, 부산의 상징에 비해 집중도가 덜하고 광안대교야경이 해운대를 앞서고 있었다. 셋째, 부산의 건축물이 아름다운가에 대한 설문에는 명확한 판단을 유보한 반면, 살기 좋은 도시인가라는 설문에는 긍정적인 답변이 지배적이었다. 부산의 길거리가 깨끗한가에 대한 설문에는 그렇지 않다는 응답이 우세하였다. 넷째, 부산사람들의 기질과 관련한 설문에서 대학생들은 부산사람들이 솔직하고 인정이 많고 화끈하며 남의 일에 관심을 표현하는 편이라고 인식하였다. 또 거칠지만 친절하고 이기적이지 않으며 개방적이며 의리가 있고 활기차다고 응답하였다. 반면 시끄럽고 무질서하며 남의 일에 간섭을 하며 성격이 급하다고 인식하고 있었다. 다섯째, 부산의 발전방향과 관련하여서는 일자리와 경제활력이 있는 도시를 가장 중요하게 꼽았으며 자신이 사는 동네에 큰 쇼핑몰이 들어오면 동네가 발전할 것이라는 인식을 보였다. 졸업 후 절반은 부산에서 그리고 30%가량은 수도권에서 일자리를 갖고 싶어했다. 앞으로 부산에 새로 생겼으면 하는 것으로는 공원>체육관>인디밴드공연장>오페라하우스>광장=연극공간의 순이었다. 부산사람들이 부산을 사랑하고 있는가에 대해서는 긍정적인 답변이 우세하였으며, 부산에 살고있는 것이 자랑스러운가에 대해서도 긍정적인 답변이 대부분이었다.