Synergistic effect of –COOH and Zr(IV) with a short distance in Zr-MOFs for promoting utilization of H2O2 in oxidative desulfurization

Zhaoyang Qi,Yan Wang,Changshen Ye,Jie Chen,Ting Qiu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

The effectiveness of the catalytic oxidative desulfurization (CODS) process crucially depends on the catalyticactivity of the catalyst and the utilization of oxidizing agent. Catalyst with multiple active sites is aneffective method to improve the CODS effectiveness. The Zr(IV) and –COOH-containing metal–organicframework, UiO-66-(COOH)2, was synthesized and used as an active and recyclable catalyst for CODS. The UiO-66-(COOH)2 shows higher catalytic activity and H2O2 utilization in the CODS process thanUiO-66/–COOH, UiO-66, and H2BDC. And the high catalytic performance is derived from the synergisticeffect between the Zr(IV) open site and free –COOH functionality within the framework. Experimentalanalysis and DFT calculations results indicate that the synergistic effect, which changes the reaction pathand protects the *O2 – radical, is a very effective strategy to promote desulfurization efficiency and the utilizationefficiency of H2O2. In addition, it is essential to highlight that the active-site distance also has asignificant influence on the effectiveness of CODS. This research may provide a new avenue for improvingthe CODS performance.

Enhanced activity of MCM-48 based tin catalyst for synthesis of 3-methylbut-3-en-1-ol by adjusting the mesochannel environment

Zhaoyang Fei,Shenglan Ai,Zhe Zhou,Xian Chen,Jihai Tang,Mifen Cui,Xu Qiao 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6

In the present work, tin incorporated MCM-48 catalysts were prepared by different strategies andapplied in the synthesis of MB-AC and MBOH. The performance of the catalysts is strongly affected bymesochannel environment and acid texture. The organic-graft method (Sn-G-MCM-48) can modify themesochannel environment and enhance the acid intensity and concentration (0.28 mmol/gcat),resulting in the improvement of the catalytic activity to the target reactions. The total yield of MB-AC andMBOH is as much as 68.4% over Sn-G-MCM-48 under mild conditions.

The Combined Effect of Sodium Butyrate and Low Culture Temperature on the Production, Sialylation, and Biological Activity of an Antibody Produced in CHO Cells

Fei Chen,Tianci Kou,Li Fan,Yan Zhou,Zhaoyang Ye,Liang Zhao,Wen-Song Tan 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6

Cell cultures containing 0 ~ 5 mM sodium butyrate (NaBu) and grown at 30 and 37°C were conducted to investigate the combined effect of NaBu and low temperature on the quantity and quality of an antibody production in CHO cells. Although NaBu addition decreased cell viability by apoptosis in a dose-dependent manner at both 30 and 37°C, the onset of significant apoptosis induced by NaBu was delayed by lowering culture temperature. The highest specific antibody productivity (q_p) of 23.26 pg/cell/day was obtained in the culture containing 2 mM NaBu at 30°C; however, the highest antibody concentration of 167.84 mg/L was achieved in the culture containing 1 mM NaBu at 30°C, as the detrimental effect of further NaBu addition on cell growth compromised its beneficial effect on q_p. Moreover, protein quality with respect to the total sialic acid content and Nglycolylneuraminic acid (Neu5Gc) level was evaluated. There were no apparent changes regarding the total sialic acid content of the antibody, but manipulation of cultures with NaBu treatment or (and) low culture temperature did decrease Neu5Gc levels by 5 ~ 10%. Biological activity of the antibody was also assessed, and no obvious changes were observed. Collectively, the simultaneous application of NaBu and low culture temperature was an effective way to extend culture period and enhance final antibody concentration,without compromising the sialic acid content or biological activity.

A Study on Geometry Design of Spiral Bevel Gears based on Conjugate Curves

Bingkui Chen,Dong Liang,Zhaoyang Li 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

A study on geometry design of spiral bevel gears based on conjugate curves is put forward in this paper. According to the theory of conjugate curves, generation principle and mathematical model of spiral bevel gears are developed for a given spiral bevel curve. The meshing equation in the given contact position for conjugate curves is derived. Tubular meshing surfaces contacting along the orientation of designated contact angle are proposed to build up the circular-arc tooth profiles, which inherit all properties of conjugate curves. Numerical example is illustrated for this research including the computerized simulation of three dimensional models and analysis of meshing characteristics. Theoretical and numerical results demonstrate the feasibility and correctness of conjugate curves theory. Compared with general spiral bevel gears, this novel gear is different in the viewpoints of meshing and generation principles. And it is expected to have more excellent transmission performance which will be further studied in future.

Preparation and properties of Joule thermal effect self-healing polyurethane

Xu Qi,Liu Yang,Chen Yu,Zhang Zhaoyang,Wei Yan Yan 한국탄소학회 2022 Carbon Letters Vol.32 No.1

Carbon nanotubes (CNTs) were added into the self-healing polyurethane materials as conductive filler, the mass fraction of carbon nanotubes was adjusted, and 1% polyaniline was doped. The conductive self-healing polyurethane composites with different carbon nanotubes content (PU)-1/3/5/8/10 were prepared, and analyzed and tested. The result shows that the permeability threshold value of the composite material is 8wt%, and the comprehensive performance of the composite material PU-8 is the best; the resistance of PU-8 is 1278Ω, PU-8P has a resistance of 1400Ω; using an infrared camera, it can be seen that the material can reach 143.3 °C under the DC current of 0.1A, reaching the temperature condition when the material is repaired; the swelling test shows that the PU-8P equilibrium swelling rate is 177%, the gel content is 52.67%, and there is no dissolution in dimethyl sulfoxide. Solvent stability is better than PU-8;DSC test shows that the glass transition temperature of the soft segment of PU-8P is 43 °C, and the glass transition temperature of the hard segment is − 55 °C, which is not much different from that of PU-8; TG test shows that the epitaxial starting temperature of PU-8P is 365 °C; the observation photo is magnified by a stereo microscope at ten times and the PU-8P sample is cut of in the middle at room temperature, applying a constant voltage of 30 V, the cracks disappeared. The material cracks realized self-healing with electricity, and the repair efficiency reached 20.5%.

Preparation and Characterization of a pH-responsive Polymer that Interacts with Microbial Transglutaminase during Affinity Precipitation

Sipeng Li,Jialing Chen,Xuanjun Zhang,Zhaoyang Ding,Xuejun Cao 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1

Microbial transglutaminase (MTG) has been widely used in the food and pharmaceuticals industries. In this study, MTG was purified using affinity precipitation with an affinity polymer (PMMDN-T), which was synthesized using a pH-responsive polymer (PMMDN) coupled with L-thyroxin as an affinity ligand. Interactions between MTG and PMMDN-T were investigated using turbidimetric titration, zeta potential measurements, and low-field nuclear magnetic resonance (LF-NMR). We found different behaviors, architectures, and phase states of pH-dependent interactions between MTG and PMMDN-T interactions. Binding energetics between MTG and PMMDN-T were determined by isothermal titration calorimetry (ITC). The isoelectric point (pI) of the affinity polymer was 4.65 and was recovered with 96.7% efficiency after recycling the polymer three times. The optimal adsorption condition was 0.02 mol/L phosphate buffer (pH 6.0) with 1.0 mol/L NaCl at 30.0°C and a ligand density of 50.0 μmol/g. The maximum elution recoveries of total MTG were 98.44% (protein) with 92.19% (activity) using 0.02 mol/L pH 10.0 Gly-NaOH as the eluent.

Comprehensive Analysis of Gut Microbiota Alteration in the Patients and Animal Models with Polycystic Ovary Syndrome

Zhou Jing,Qiu Xuemei,Chen Xuejing,Ma Sihan,Chen Zhaoyang,Wang Ruzhe,Tian Ying,Jiang Yufan,Fan Li,Wang Jingjie 한국미생물학회 2023 The journal of microbiology Vol.61 No.9

Polycystic ovary syndrome (PCOS) is a common disease of endocrine–metabolic disorder, and its etiology remains largely unknown. The gut microbiota is possibly involved in PCOS, while the association remains unclear. The comprehensive analysis combining gut microbiota with PCOS typical symptoms was performed to analyze the role of gut microbiota in PCOS in this study. The clinical patients and letrozole-induced animal models were determined on PCOS indexes and gut microbiota, and fecal microbiota transplantation (FMT) was conducted. Results indicated that the animal models displayed typical PCOS symptoms, including disordered estrous cycles, elevated testosterone levels, and ovarian morphological change; meanwhile, the symptoms were improved after FMT. Furthermore, the microbial diversity exhibited disordered, and the abundance of the genus Ruminococcus and Lactobacillus showed a consistent trend in PCOS rats and patients. The microbiota diversity and several key genera were restored subjected to FMT, and correlation analysis also supported relevant conclusions. Moreover, LEfSe analysis showed that Gemmiger, Flexispira, and Eubacterium were overrepresented in PCOS groups. Overall, the results indicate the involvement of gut microbiota in PCOS and its possible alleviation of endocrinal and reproductive dysfunctions through several special bacteria taxa, which can function as the biomarker or potential target for diagnosis and treatment. These results can provide the new insights for treatment and prevention strategies of PCOS.

Operation Safety Risk Assessment of Water Distribution Networks Based on the Combined Weighting Method (CWM)

Wei Liu,Wanli Cui,Mingji Chen,Qunfang Hu,Zhaoyang Song 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.5

This study proposes the risk assessment framework of water distribution networks with a focus on the determination of weights of various risk indices. First, the pipe risk list is built, in which pipe failure probability and consequence are considered. By integrating the analytic hierarchy process (AHP) and the entropy weighting method (EWM), the weights of these indices are obtained by the combined weighting method (CWM), by which both human experience and data distribution can be considered comprehensively. Taking a real WDN in an industrial zone in China as an example, the proposed method is demonstrated in detail and the risks of all pipes are determined. The accuracy of the CWM is validated from the perspective of risk levels of pipes with historical failure records. It is found that the AHP or EWM underestimates or overestimates pipe risks, respectively. Compared to them, the CWM provides reasonable results. In addition, the risks of plastic pipes, newly buried pipes less than five years old, and pipes over twenty years old are much higher.

Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1

( Qiuke Hou ),( Yongquan Huang ),( Yan Wang ),( Liu Liao ),( Zhaoyang Zhu ),( Wenjie Zhang ),( Yongshang Liu ),( Peiwu Li ),( Xinlin Chen ),( Fengbin Liu ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10

Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which microRNAs and lactobacilli exert their beneficial effects on intestinal epithelial permeability. Hence, to elucidate whether miRNAs and lactobacilli play roles in intestinal epithelial barrier regulation, we compared miRNA expression levels in intestinal epithelial cells (IECs) under Lactobacillus casei (L. casei LC01) treatment. IECs and L. casei LC01 were co-cultured and then subjected to microRNA microarray assay. qRT-PCR, western blot and ELISA were used to detect the expression of occludin (OCLN) and zonula occludens 1 (ZO1/TJP1). The interaction between miRNAs and L. casei LC01 acting in IECs was investigated through transfection of RNA oligoribonucleotides and pcDNA 3.1 plasmid. The results are as follows: 1) L. casei LC01 decreased the expression of miR-144 and FD4 and promoted OCLN and ZO1 expression in IECs; 2) L. casei LC01 enhanced the barrier function of IECs via downregulation of miR-144 and upregulation of OCLN and ZO1; 3) Under L. casei LC01 treatment, OCLN and ZO1 overexpression could partially eliminate the promoting effect of miR-144 on intestinal permeability in IECs. Our results demonstrate that L. casei LC01 regulates intestinal permeability of IECs through miR-144 targeting of OCLN and ZO1. L. casei LC01 can be a possible therapeutic target for managing dysfunction of the intestinal epithelial barrier.

Dynamic recrystallization and microstructure evolution of a Nb-V microalloyed forging steel during hot deformation

Zhao, Yang,Chen, Liqing,Liu, Xianghua Techno-Press 2014 Advances in materials research Vol.3 No.4

In this study, a forging steel alloyed with both Nb and V was used as experimental material and the hot deformation behavior has been studied for this steel by conducting the compressive deformation test at temperature of $900-1150^{\circ}C$ and strain rate of $0.01-0.01s^{-1}$ in a MMS-300 thermo-mechanical simulator. The microstructure evolution, particularly the dynamically recrystallized microstructure, of the experimental steel at elevated temperatures, strain rates and strain levels, was characterized by optical microstructural observation and the constitutive equation in association with the activation energy and Zener-Hollomon parameter. The curves of strain hardening rate versus stress were used to determine the critical strain and peak strain, and their relation was connected with Zener-Hollomon parameter. Under the conditions of processing temperature $900^{\circ}C$ and strain rate $0.01s^{-1}$, the dynamic recrystallization took place and the austenite grain size was refined from $164.5{\mu}m$ to $28.9{\mu}m$.