아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

Safety of a Traditional Korean Medicine, Cheonggan extracts (CGX)

Jang-Woo Shin,Jung-Hyo Cho,Dong-Seok Seo,Nak-Won Sung,Min Kwon,Chang-Gue Son 대한한의학회 2009 대한한의학회지 Vol.30 No.6

Safety of a Traditional Korean Medicine, Cheonggan extracts (CGX): A 2-week Single-dose Toxicity Study in SD Rats and Beagle Dogs

Shin, Jang-Woo,Cho, Jung-Hyo,Seo, Dong-Seok,Sung, Nak-Won,Kwon, Min,Son, Chang-Gue The Society of Korean Medicine 2009 대한한의학회지 Vol.30 No.6

Objectives: To evaluate the acute toxic effects and approximate lethal dose of Cheonggan extracts (CGX) in SD rats and beagle dogs. Methods: Male and female rats were divided into 4 groups (Control, CGX 1250, CGX 2500, CGX 5000) respectively and male and female dogs were divided into two groups respectively (Control, CGX 5000) respectively. A single oral dose of CGX was treated to the rats and dogs. Mortality, signs of gross toxicity, and behavioral changes were observed over 14 days. All animals were observed every hour for 4 hours after administration and once a day thereafter for 14 days. Body weights were determined at $0_{th}$, $7_{th}$, and $14_{th}$ days. All surviving animals were sacrificed and necrotized. Major organs were inspected visually for gross findings. Results: No animals died in any of the groups during the experimental period (2 weeks), rats or dogs. Body weights of rats and dogs during the experiment continuously increased in all groups but there was no significant change. No abnormal clinical signs were observed for 2 weeks after a single administration of CGX in any dose group of CGX, rats or dogs. No abnormal findings in major organs were observed in any group of rats or dogs. Conclusion: CGX does not have acute toxic effects in rats or dogs. Therefore, an approximate lethal dose is assumed to exceed 5000 mg/kg in both rats and dogs.

급성 심근 경색중 후 추적 관찰에 관한 연구

박영배,이영우,오동진,고창순,서정돈,서봉관,오병회 대한내과학회 1986 대한내과학회지 Vol.31 No.5

To assess the change in left ventricular function after hospital discharge, 57 patients with acute myocardial infarction were studied before discharge and 26 of them after discharge as well with either or both of treadmill test and radionuclide ventriculography(RNV). Follow-up study patients were 2g men with mean age of 53.1. Their infarct locations were anterior in 16 cases, inferior in 8 cases and anterior and inferior in 2 cases; 21 cases were in Killip class I, 3 cases in II, and 2 cases in III. Follow-up study was performed 2~19 months(mean 7.3 months) after acute myocardial infarction. The results were as follows: 1) Predischarge study showed that a relationship is present between regional wall motion abnormality, Killip class, peak serum CK and left ventricular ejection fraction(LVEF). 2) Predischarge and follow-up treadmill tests showed no significant change. 3) Follow-up RNV showed worsening of regional wall motion only in 1 case: Other cases showed either improvement or no change in regional wall motion. 4) Overall patients showed a significant increase in LVEF on follow-up. By location, only anterior infarction showed a significant increase in LVEF. 5) Killip class I patients showed a significant increase in LVEF on follow-up. 6) Most cases in this study were uncomplicated myocardial infarction and they showed improved LV function(suggested by RNV) on follow-up. Treadmill test also seems to be valuable for evaluationg improvement in exercise capacity on follow-up. So it is recomended that treadmill test or RNV be performed after discharge to evaluate change in LV function objectively.

Load-cell Sensor를 이용한 산지 토사유출량 계측의 현장 적용성 검토

서준표(Jun-Pyo Seo),이기환(Ki-Hwan Lee),김종엽(Dong-Yeob Kim),우충식(Choong-Shik Woo),이창우(Chang-Woo Lee),이헌호(Heon-Ho Lee) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.1

최근 기후변화로 인한 집중호우, 태풍 등의 영향으로 산사태가 빈번히 발생하고 있다. 산사태를 예방할 수 있는 효율적인 방법은 사방사업이다. 이러한 사방사업의 효율성을 높이기 위해서는 산지에서 유출되는 토사량에 대한 정량적 측정이 필요하다. 본 연구에서는 산지계류에 Load-cell 센서를 설치하고, 분동테스트를 통하여 계류에서의 적용성과 재하 형태에 따른 출력값을 비교하였다. 개별 및 복합 하중시험을 실시한 결과, Site 1에서 평균 0.4kgf, Site 2에서 평균 0.6kgf 차이로 재하 형태의 영향은 낮게 나타났다. 또한 측정값의 정확도를 높이기 위해 하중계수를 산정하였다. 하중계수를 Load-cell 측정값에 적용하여 보정한 결과, 두 사이트에서 출력율이 각각 14.8%p, 24.6%p 상승하여 기준값에 근사하게 산정되었다. Load-cell 센서는 수위, 강우 등과 함께 시계열 분석을 통하여 산지 토사유출량을 정량적으로 산정할 수 있었다. 모니터링이 장기간 이루어진다면 산림유역에 대한 토사유출 매커니즘 구명에도 활용할 수 있을 것이다. 또한 Load-cell과 같은 센서를 산지 계곡에 적용함으로써 계측센서 등 관련분야 산업 발전에 기여할 수 있을 것으로 기대된다. Landslides occur frequently due to the effects of heavy rainfall and typhoons caused by climate change. Erosion control measures are needed to effectively prevent landslide damage. In order to improve their efficiency, it is necessary to quantitatively measure the sediment discharge from the mountain stream. In this study, a load cell sensor was installed in a mountain stream and the measured values were compared according to the applicability and load test type in the mountain stream. The result of the load test showed that the effect of the loading type (load test 1, 2) was low at average (loadings) of 0.4kgf and 0.6kgf at sites 1 and 2, respectively. The load factor was also derived by regression analysis to increase the accuracy of the measured values. According to the results of the load factor (normalized) to the load-cell measurement value, the output value increased by 14.8% and 24.6% in sites 1 and 2, respectively, and was calculated to be similar to the reference value. The load cell sensor enabled us to quantitatively estimate the amount of sediment discharge in the mountain stream through time series analysis with the water level and rainfall information. If the monitoring is carried out for a long time, it can be used to find the sediment discharge mechanism for the mountain stream. In addition, applying sensors such as load-cells to a mountain stream is expected to contribute to the development of related industries, such as the manufacturing of measurement sensors.

기후변화 시나리오에 따른 지역별 확률강우량

김영호,여창건,서근순,송재우,Kim. Young-Ho,Yeo. Chang-Geon,Seo. Geun-Soon,Song. Jai-Woo 한국방재학회 2011 한국방재학회논문집 Vol.11 No.3

본 연구는 A1B 기후변화 시나리오를 고려하여 지역별 확률강우량을 산정하고 관측소별 기존 관측자료의 특성을 고려한 적정 방법을 제안하였다. 이를 위하여 우리나라 주요 지점 강우관측소를 연구 대상지점으로 선정하여 선정된 주요 지점 관측소에 대해 24시간 연최대강우량 평균값과 매개변수의 관계를 분석하여 2100년의 빈도별 확률강우량을 산정하였다. 2100년 빈도별 확률강우량은 기상청 실측강우량 자료를 활용하여 산정하는 방법, 확률분포의 매개변수는 실측 강우자료를 활용하고, 2100년까지의 강우자료는 A1B 시나리오를 활용하는 방법, A1B 시나리오를 활용하여 확률강우량을 산정하는 3가지 방법을 적용하였다. A1B 시나리오에 의한 강우 예측값은 실측값 보다 과소 추정되어 이를 활용하는 경우에는 보정이 필요하며, 분위 사상법을 적용하여 보정한 결과 모든 관측소에서 약 2.3~3.0배의 강우량이 평균적으로 상향조정 되었다. 실측강우 자료만으로 산정한 확률 강우량의 경우, 강우량이 지속적으로 증가하여 과대 산정되어 증가하는 경향이 강하며, A1B 시나리오 자료를 활용하여 산정한 확률강우량의 경우 대체적으로 기존 관측자료의 증감율과 유사하게 산정되기는 하지만 지역적 특성을 정확히 반영하지 못하는 경우가 다소 발생하였다. 각 지점별로 24시간 연 최대 강우량 평균 증가율과 방법별로 산정된 확률강우량의 증가율을 비교하여 기후변화를 고려한 관측지점별 확률강우량 산정 방법을 선정하였다. This research proposes the suitable method for estimating the future probable rainfall based in 2100 on the observed rainfall data from main climate observation stations in Korea and the rainfall data from the A1B climate change scenario in the Korea Meteorological Administration. For all those, the frequency probable rainfall in 2100 was estimated by the relationship between average values of 24-hours annual maximum rainfalls and related parameters. Three methods to estimate it were introduced; First one is the regressive analysis method by parameters of probable distribution estimated by observed rainfall data. In the second method, parameters of probable distribution were estimated with the observed rainfall data. Also the rainfall data till 2100 were estimated by the A1B scenario of the Korea Meteorological Administration. Last method was that parameters of probable distribution and probable rainfall were estimated by the A1B scenario of the Korea Meteorological Administration. The estimated probable rainfall by the A1B scenario was smaller than the observed rainfall data, so it is required that the estimated probable rainfall was calibrated by the quantile mapping method. After that calibration, estimated probable rainfall data was averagely became approximate 2.3 to 3.0 times. When future probable rainfall was the estimated by only observed rainfall, estimated probable rainfall was overestimated. When future probable rainfall was estimated by the A1B scenario, although it was estimated by similar pattern with observed rainfall data, it frequently does not consider the regional characteristics. Comparing with average increased rate of 24-hours annual maximum rainfall and increased rate of probable rainfall estimated by three methods, optimal method of estimated future probable rainfall would be selected for considering climate change.

제한효소 DNA 분석법에 의한 국내분리 렙토스피라균의 동정

장우현, 김석용, 서정선 忠北大學校 遺傳工學硏究所 1988 遺傳工學硏究 Vol.2 No.1

본문참고

鬱血性心不全症에 對한 臨床的 觀察

徐明洙,金吉洙,金佑泰,張錫源,金承源,宋昌燮 대한적십자사 1983 적십자병원지 Vol.10 No.2

구강 편평세포암종에서 E-cadherin 및 β-catenin 발현의 변화 : Correlation with Histologic Features and p53 Expression

서진건,권창석,박진배,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Altered expression of cell adheion molecules is associated with biologic behavior of tumor. The aim of this study is to evaluate the expression pattern of E-cadherin, β-catenin and p53 protein according to histologic grade and invasion pattern of squamous cell carcinoma of the oral cavity and the relationship between E-cadherin, β-catenin and p53 protein expression. Methods and Material : The materials were fifty seven cses of squamous cell carcinoma, and clinicl parameers such as age, sex, tumor location, stage and recurrence were recorded. Histologic review was done based on histologic grade and invasion pattern(nodular vs infiltrative). Immunohistochemical stains for E-cadherin and β-ca tenin were interpreted based on staining pattern as los of membranous expression and cytoplasmic expression and p53 protein expression was defined as positive if more than 10% of nuclei were reactive. Statistical analysis between E-cadherin, β-catenin and p53 protein expressions and histologic grade and invasion pattern and the relationship between E-cdherin, β-catenin and p53 protein expressions were perfomed. Results : There wa positive correlation between higher histologic grade and infiltrative pattern. Loss of membranous expression and cytoplasmic expression of E-cadherin were noted in 33.3% and 57.9%, respectively, and loss of membranous expression of E-cadherin showed increaing tendency in poorly differentiated carcinomas, however, there was no significant difference of cytoplasmic expression rate of E-cadherin according to histoogic grade. Altered expression of E-cadherin was more frequent in poorly differentiated carcinomas. Loss of membranous expression and cytoplasmic expression of E-cadherin were more frequent in carinomas with infiltrative pattern than in carcinomas with nodular pattern, but their diferences were not significant, Loss of membranous expression and cytoplasmic expression of β-catenin were observed in 19.3% and 80.7%, respectively. Loss of membranous expression of β-catenin howed no positive correlation according to histologic grade and invasion pattern, but cytoplasmic expression rate of β-catenin was higher in poorly differentiated carcinomas and in carcinomas with infiltrative pettern. p53 protein expression rate was 45.6%, and showed invreasing tendency in poorly differentiated carcinomas, but no significant relationship with invasion pattern. There was an inverse relationship of loss of membranous expression and cytoplasmic expression of E-cadherin and β-catenin. Altered expression of E-cadherin was related to cytoplasmic expression of β-catenin, however, there were no significant relationship between altered expressions of E-cadherin and β-catenin and p53 protein expression. Conclusion : In squamous cell carcinomas of the oral cavity, altered expression of E-cadherin and β-catenin was more frequent in poorly differentiated carcinomas and in carcinomas with infiltrative pattern and there was positive correlation betwween altered expression of E-cadherin and β-catenin. These findings suggest that altered expressions of E-cadherin and β-catenin may have a role in the development of squamous cell carcinomas with aggressive biologic behavior. but altered explosions of E-cadherin and β-catenin. These findings suggest that altered expressions of E-cdherin and β-catenin my have a role in the development of squmaous cell carcinomas with aggressive biologic behavior. But altered expression of E-cadherin and β-catenin might not be related to p53 protein expression. Further study on genetic mutation related to altered expression of E-cadherin and β-catenin will be needed

CMP 공정의 재현성 확보를 위한 공정제어 범위의 결정

서용진,정소영,김철복,박성우,이경진,김기욱,박창준 대불대학교 2002 大佛大學校大學院 硏究論文集 Vol.- No.1

To achieve the ULSI goals of higher density and greater performance, STI(shallow trench isolation)-CMP(chemical mechanical polishing) process has been attracted. Recently, the direct STI-CMP process without the conventional complex reverse moat etch process has established by using slurry additive with the high selectivity between SiO2 and Si3N4 films for the purpose of process simplification and in-situ EPD(end point detection). However, STI-CMP process has various defects such as nitride residue, torn oxide and demage of silicon active region. Also, it was difficult to assure the suitable process margin in the STI-CMP process. To solve these problems, in this paper, we discussed to determine the control limit of process, which can entirely remove the oxide on nitride film from the most area of high density as reducing the damage of dense moat area and minimizing dishing effect in the large field area. We, also, evaluated the wafer-to-wafer thickness variation and the day-by-day reproducibility of STI-CMP process after repeatable tests.