상피성 암세포주에 대한 녹차 Catechin의 효과

박정현(Jeong Hyun Park),김대중(Dae Joong Kim),한장희(Jang Hee Hahn),김홍태(Hong Tae Kim),정용욱(Yong Wook Jung),성훈기(Hoon Ki Sung),김주영(Joo Young Kim),송인환(In Hwan Song),성언기(Eon Gi Sung),이융창(Yung Chang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.5

Catechin은 녹차에서 추출되는 폴리페놀의 주요성분으로 고혈압 및 동맥경화의 예방효과, 당뇨억제효과, 항산화작용, 항암작용 등에 직접 관여하는 물질이다. 본 연구에서는 태평양에서 제공받은 녹차 catechin을 이용하여 상피성 암세포주인 A549 (폐암)와 EATC (복수암)세포에 투여한 후 처리농도와 시간에 따른 변화를 비교, 관찰함으로써 녹차의 효과와 작용기전을 밝혀내는데 목적이 있었다. 본실험은 A549 세포와 EATC 세포를 배양한 후 녹차 catechin을 1, 10, 100, 500 μg/ml의 농도로 48시간 동안 처리하였고, 광학현미경, 공초점현미경, 전자현미경 등을 이용하여 세포의 구조적 변화를 확인하였으며 MTT분석, 전기영동, 유세포분석기 등을 사용하여 세포 손상정도를 파악하였다. A549 세포에서는 catechin 1 μg/ml와 10 μg/ml 농도에서는 대조군에 비하여 큰 변화를 관찰할 수 없었다. 100 μg/ml catechin을 처리하였을 때 세포내의 검은 과립들의 수가 증가하였고 층판소체의 손상이 나타났다. 세포주기의 장애가 나타나 DNA 합성전기에 있는 세포들의 수가 급격히 증가하였다. 500 μg/ml 농도에서는 층판소체와 사립체의 파괴가 심하게 나타났으며 세포생존율이 감소하였고 세포주기의 장애도 관찰되었다. EATC 세포에서는 catechin의 농도가 A549 세포의 경우보다 낮은 농도에서도 세포증식 억제 및 세포손상 효과가 나타났다. 10 μg/ml 농도에서도 세포의 위축과 생존율의 감소가 일어났으며 전기영동상에 괴사되는 세포들이 파악되었다. 100μg/ml catechin을 처리하였을 때 자연사의 형태학적 관찰, 전기영동, 유세포분석 등에서 자연사 과정에 있는 세포들이 많이 나타났다. 결과적으로 녹차 catechin을 배양한 상피성 암세포에 투여함으로서 세포의 생존율과 증식이 억제되었고 그 과정에서 괴사, 자연사, 세포주기의 장애 등이 관여하는 것으로 생각된다. 그러나 일련의 세포손상과정을 유도하는 데 있어 세포의 종류, 처리 시간, 농도에 따라 다소 차이가 있음을 확인하였다. Catechin is main component of polyphenol extracts from green tea, it is associated with prevention of hypertension and atherosclerosis, anti-diabetic effect, antioxidant, antitumor. The purpose of this research is to investigate the effect and its mechanism of green tea catechin on epithelial cancer cell lines in various concentrations and durations. For this study, epithelial cancer cell lines, A549 (lung cancer), EATC (Ehrlich-Lettre ascites tumor cell) were used. Inverted, light, confocal and electron microscopes were applied to find morphological changes. MTT assay, flowcytometric analysis, gel electrophoresis were used to compare severity of cellular damages to control after exposure to 1, 10, 100 and 500 μg/ml catechin for 48 hours. In the A549 cells, after 1 μg/ml and 10 μg/ml catechin treatments, there was no notable changes. However, exposure to 100 μg/ml catechin induced increase of cytoplasmic granules, destruction of lamellar body, inhibition of cell cycle, especially G0/G1. In the early phase of 500 μg/ml catechin administration, decrease of cell population, severe destruction of lamellar bodies and mitochondria, derangement of cell cycle were shown. In the EATC, such as those effects occurred after exposure to lower concentration of catechin than in that of A549 cells. After exposure of 10 μg/ml catechin, rounded-up cells and necrotic cells were found. Whereas, most of cells were under apoptotic changes-cytoplasmic condensation, nuclear fragmentation, cellular shrinkage, ladder pattern in the electrophoresis, when administrated 100 μg/ml catechin. These results suggested that exposure of catechin induced severe cellular damage and growth inhibition in dose- and time-dependent manner. And we confirmed that these effects of catechin were involved with apoptosis, necrosis and cell cycle arrest and were quite different according to cancer type. Therefore, much more research would be demanded before clinical application of catechin to human cancer therapy and this study would be the basic source for further study of green tea.

농촌지역 여성고령자의 현미덤벨체조가 체조성, 체력 및 식습관에 미치는 영향

이기철 ( Lee Gi Cheol ),정창주 ( Jeong Chang Ju ),손기수 ( Son Gi Su ),우임수 ( U Im Su ),이원경 ( Lee Won Gyeong ),공무현 ( Gong Mu Hyeon ),이수천 ( Lee Su Cheon ) 한국운동영양학회 2003 Physical Activity and Nutrition (Phys Act Nutr) Vol.7 No.2

The present study evaluated the possibility of dumbbell exercise as to improve the physical fitness and the health starus of old people in rural area. Although eighty seven volunteers of old women (over 64) living in Seonsan, Gumi city were subjected, twenty of them were attended the whole program for 6 months and the data from the twenty was analysed in this study. The results were drawn out from comparison of the body constitutuion, the physical strength, the medical aspectrs, and the questionaires of before and after this program. They showed increased physical fithess tested by grip strength (left), bar gripping reaction time, standing trunk flexibility, on foot standing test through dumbbell exercise for 6 months. Questionaire revealed the betterment of dietary pattem during this program. In the light of in these results, it is possible to conclude that dumbbell exercise had improved the basic physical condition and the health status through the increasement of physical fitness and dietary pattem change. Therefore, dumbbell exercise would be a useful program for the old people in reual area to improved their health status.

예천지역 화강섬록암 풍화대내 흑운모의 Rb-Sr 동위원소연대 변화

정기영,정창식,이봉호 한국광물학회 2005 광물과 암석 (J.Miner.Soc.Korea) Vol.18 No.1

예천지역 화강섬록암 풍화단면내의 흑운모가 산화흑운모로 풍화되는 과정에서 Rb-Sr 동위원소연대의 변화를 분석하였다. 신선한 흑운모와 풍화된 흑운모들의 Rb-Sr 동위원소 조성을 열이온 화질량분석기로 측정하였고, 이를 기존의 K-Ar 자료와 비교하였다. Rb-Sr 동위원소연대는 흑운모의 산화정도에 따라 체계적으로 감소하는 경향을 보이며, 이는 K-Ar 연대의 감소경향과 잘 일치하였다. Fe^(²+)의 산화로 발생하는 과잉전하로 구조내 양이온들이 방출되는 과정에서 층간의 방사기원 핵종도 일부 방출되었는데, 일가 양이온인 ^(87)Rb이 이가 양이온인 ^(87)Sr로 붕괴되어 딸핵종인 ^(87)Sr이모핵종인 ^(87)Rb보다 심하게 흑운모로부터 제거된 것으로 보이고, 그 결과 Rb-Sr 동위원소연대가 감소하였다. 풍화된 흑운모는 모암의 연대측정대상으로 부적합한 것으로 보이지만, 풍화과정에서 방사기원 동위원소들의 거동은 흑운모 풍화의 지화학적 및 구조적 과정에 대한 유용한 정보를 제공 한다. Rb-Sr isotopic ages of oxidized biotite in the weathering profile of granodiorite, Yecheon area, were measured by thermal ionization mass spectrometry, and compared with their K-Ar ages. A decrease of Rb-Sr isotopic age is well correlated with iron oxidation, and consistent with K-Ar age. Octahedral and interlayer cations including Rb and Sr were partly released from the oxidizing biotite by excess positive charge from iron oxidation. Divalent ^(87)Sr decayed from monovalent ^(87)Rb was more easily released from biotite, resulting in the reduction of Rb-Sr isotopic age. Weathered biotite is not suitable for the age dating of parent rocks, but behaviour of radiogenic isotopes provides useful information on the geochemical and structural changes of biotite during weathering.

소나무 재생버섯(Fomitopsis pinicola Jeseng) 다당류의 추출 특성

장경호,신진기,이명예,이상일,김정숙,오승희,김순동 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

This study was conducted to investigate the extraction characteristics of the polysaccharide from Jeseng mushroom (Fomitopsis pinicola Jeseng). Yields of the polysaccharide extracted from powdered mushroom by autoclaving(120, 30 min) with water at different pH and salt concentration were 8.2~9.2% in pH 5~11, 4.7~5.5% in 1.5% salt solution, respectively. The yield by the 0.05~1.0 N KOH-extraction was ranged 3.45~13.20%, while that by HAS-extraction(homogenizing after KOH swelling) using 1~2.5 N KOH 73.6~78.4%. Content of carbohydrate, protein, lipid and ash of the crude polysaccharide extracted from fruits body and its cultured mycelium by method of water extraction, KOH extraction(0.005~1N) and HAS-extraction were ranged 86.5~92.6%, 2.3~13.1%, 0.1~4.2% and 0.1~1.7%, respectively. The polysaccharide were composed of 62.0~77.8 g/g of pentose, 138.0~187.8 g/g of hexose and 21.2~117.3 ㎎/g of protein. From these results, the polysaccharide extracted was supposed to be a protein-bound polysaccharide.

갑상선 항진증에 관한 외과적 치료의 임상적 연구

장일성,최길돈,하기정 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

A clinical study of hyperthyroidism was performed for 20 patient who received operation at the department of surgery of C. N. U. H. from March, 1973 to July, 1987. The results were as follows: 1) In clinical classification, 14 cases (80%) were diffuse toxic goiter and 6 cases (20%) were nodular toxic goiter. 2) Male to female ratio was 1 : 19 and the highest incidence in the 3rd decade. 3) Main symptoms and signs were, in the order of decreasing frequency, neck mass (95%), palpitation (85%), fatigability (80%), heat intolerance (75%), weight loss (65%), tremor (60%) headache (50%). 4) Pulse rate changed from 130 per minute preoperatively to 60 postoperatively. 5) Thyroid function test revealed high level above the normal in 16 (80%) and the mean value of T_3RI_4 4.06mg/ml, T_3RI_4 10.48mg/ml, free T_4 4.65/ug/dl, postoperatively all cases showed teuthyroid function. 6) Electro-cardiographic findings were sinus tachycardia 9 cases (45%), normal 6 cases (30%). sinus bradycardia 5 cases (25%). 7) In preoperative preparation, Lugol solution was more frequently used 8 cases (40%) and others prophylthiouracil plus lugol solution 4 cases (20%), Inderal plus lugol solution 4 cases (20%) Methimazole plus lugol solution 2 cases (10%), prophylthiouracil plus Inderal plus comthyroid plus lugol solution 2 cases (10%). Average mean duration of preoperative preparation was 12.8 days. 8) The types of operation were in the order of decreasing frequency near total thyroidectomy 9 cases (45%), subtotal thyroidectomy 6 cases (30%), Lobectomy 4 cases (20%) and total thyroide ctomy 1 case (5%), 9) Early postoperative complication were transient tremor 3 cases (15%) transient hoarseness 1 case (5%), hypothyroidism 1 case (5%), recurrent hyperthyrodism 1 case (53%), hypoparathyroidism l. case (53%) but there was no case of operative motality.

형질전환 제초제저항성 벼 밀양 204호의 농업적 특성

정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

This study was conducted to investigated the major characteristics of genetically modified rice of "Milyang 204" originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Junambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of "Milyang 204" showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic "Milyang 204" showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the nontransgenic varieties rice.

양돈폐수로부터의 수소 생성 특성

장영복,정태영,차기철,정형근,김성헌,김동진,유익근 한국물환경학회 2004 한국물환경학회지 Vol.20 No.4

The characteristics of H₂ production from livestock wastewater were investigated through an anaerobic acid forming process using Clostridium beijerinckii and the photosynthetic process using Rhodobacter sphaeroides. The submerged separation membrane was installed in the acid forming reactor. The photosynthetic process is composed of two reactors(photosynthetic reactor 1 and photosynthetic reactor 2) which is connected continually. The removal rate of COD and the production of volatile fatty acid(VFA) in the acid forming process were approximately 50% and 1000㎎/ L, respectively. The 70% of COD in the effluent of acid forming process was removed through the photosynthetic process. The production of H₂ in the photosynthetic reactor 1 and 2 was 50 and 25mLH₂/gVFA_(COD), respectively. The values of Y in acid forming reactor, photosynthetic reactor 1 and 2 was 0.2263, 0.0601 and 0.0393, respectively. The acetic acid and butyric acid produced in acid forming process were converted to H₂ by photosynthetic bacteria.

Si 웨이퍼 내의 깊은 준위 분포 특성 파악에 관한 연구

홍창희,윤기정,안기형 동아대학교 공과대학 부설 한국자원개발연구소 1988 硏究報告 Vol.12 No.2

Distributions of deep levels are studied in the n-type Si wafer which are introduced during fabrication with e-beam deposition by using DLTS technique. Test is carried out on the Schottky diode. Metalization of the diode is done by e-beam deposition before of after electron irradiation. Deep levels of the defects are measured by using various metal such as Pd/Au, Au, Pd, Ti, Mo and Hf, and by changing the annealing temperature. It is approved that some defects are introduced in the neighbor of interface of silicon and metal by e-beam deposition, and main defect of them is E(0.42) level. In the resuts, two types of the deep level are observed ; one type is that is independent of electron irradiation, for example, E(0.42) level which is observed near the interface ; another type is that is observed only in the diode deposited by e-beam after electron irradiation, for example, E(0.18) level and so on which are observed farther from the interface. It may be the reason that interaction is presented between defects from Pd, at least, and prior defects.

뇌경색 환자의 혈중 호모시스틴치 (예비 연구)

정두신,송인욱,조상걸,성기범,박형국,신현길,장성근 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.