Application of a wet oxidation method for the quantification of ³H and ¹⁴C in low-level radwastes

Ahn, H.J.,Song, B.C.,Sohn, S.C.,Lee, M.H.,Song, K.,Jee, K.Y. Pergamon Press ; Elsevier Science Ltd 2013 Applied radiation and isotopes Vol.81 No.-

Wet oxidation methods are utilized to separate and quantify <SUP>3</SUP>H and <SUP>14</SUP>C radionuclides in inorganic and organic radwastes generated at nuclear facilities. Because <SUP>3</SUP>H and <SUP>14</SUP>C are pure beta emitters with half-lives of 12.3 and 5,730 years, respectively, these radionuclides should be chemically separated from other radionuclides present in radwastes for accurate quantification. In particular, a collection technique for <SUP>14</SUP>C radionuclide in radwastes is needed because it is converted into <SUP>14</SUP>CO<SUB>2</SUB> gas by an oxidation reaction. To confirm the recoveries of <SUP>3</SUP>H and <SUP>14</SUP>C, various standard radioactive sources were used to verify the proposed method. Because the majority of <SUP>3</SUP>H radionuclides are distributed in tritiated water (HTO), only tritiated water was used as a standard for <SUP>3</SUP>H radionuclides. Additionally, <SUP>14</SUP>C-labeled methanol (<SUP>14</SUP>CH<SUB>3</SUB>OH), lauric acid (<SUP>14</SUP>CH<SUB>3</SUB>(CH<SUB>2</SUB>)<SUB>10</SUB>COOH), sodium bicarbonate (NaH<SUP>14</SUP>CO<SUB>3</SUB>), and toluene (C<SUB>6</SUB>H<SUB>5</SUB><SUP>14</SUP>CH<SUB>3</SUB>) were used as <SUP>14</SUP>C standards. The compounds were oxidized with chemical oxidants and then separated. The individual species were mixed with a scintillation cocktail and counted using a liquid scintillation counter. The recoveries of <SUP>14</SUP>C and <SUP>3</SUP>H were 82-97% and 98%, respectively. The wet oxidation method will be applied to RI wastes for clearance.

The Use of Collagen Content as Determined by Spectral Domain Polarization-Sensitive Optical Coherence Tomography to Assess Colon Anastomosis Healing in a Rat Model

Son, K.H.,Jeong, H.-W.,Jung, W.-W.,Kim, H.-S.,Lee, S.K.,Kim, K.T.,Ahn, C.B.,Park, K.Y.,Kim, B.-M.,Lee, S.H. S. Karger AG 2014 European surgical research Vol.52 No.1

<P>Abstract</P><P><B><I>Background/Purpose:</I></B> Many studies have been undertaken to prevent anastomosis leakage of the colon, and several methods have been used to assess anastomosis healing, such as measurement of bursting pressure or hydroxyproline (a marker of collagen) content at the anastomosis site. However, these methods are inappropriate for comparing anastomosis healing at two time points in the same animals. In the present study, we measured the collagen level by spectral domain polarization-sensitive optical coherence tomography (SD-PS-OCT) to assess anastomosis healing. <B><I>Methods:</I></B> Sprague-Dawley rats were divided into groups C (saline-administered controls; study group) and M [a 5-fluorouracil (5-FU)-administered experimental group]. Immediately after end-to-end anastomosis of the colon, SD-PS-OCT images of anastomoses were taken (baseline). Animals were administered saline or 5-FU for 7 days. On the 7th postoperative day, SD-PS-OCT images were acquired, a histopathologic exam was performed, and hydroxyproline levels as well as mRNA expressions of collagen-1 and collagen-3 were measured at the anastomosis site. <B><I>Results:</I></B> Fibroblast proliferation and inflammatory cell infiltration were greater in group C than in group M. The mRNA expressions of collagen-1 and collagen-3 were substantially higher in group C. Hydroxyproline levels were higher in group M than in group C. Though collagen levels measured by SD-PS-OCT at 7 days were elevated compared with baseline in group C, no such changes were observed for group M. <B><I>Conclusion:</I></B> Collagen levels at the colon anastomosis site, measured with SD-PS-OCT, were not increased at 7 days postoperatively versus baseline when 5-FU was injected, but were increased in saline-treated controls. The measurement of collagen content by SD-PS-OCT was found to provide a good means of assessing anastomosis healing, because it allows in situ assessment of collagen contents at baseline and during the postoperative period.</P><P>© 2014 S. Karger AG, Basel</P>

국내에서 비육한 수입 생우의 도체등급 특성에 관한 연구

박범영,조수현,김진형,이왕식,김용곤,안종남,이종문,윤상기 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

국내에 생우로 수입된 호주산 육우 Hereford, Angus, Murray Grey, Hereford X Angus 각각 10두의 도체등급 특성을 비교 분석하여 수입생우의 국내 비육에 대한 기초자료를 제시하고자 실시하였다. 육량등급요인 중 등지방 두께는 Hereford종이 13.7㎜로 가장 얇았으며, Angus, Murray Grey, Hereford X Angus은 각각 18.20, 18.90, 17.30㎜로 나타났다(p<0.05). 배최장근 단면적에서는 품종간 유의적인 차이가 나타나지 않았으며(p<0.05), 육량지수는 Hereford종이 64.63으로 가장 높았고, Murray Grey종이 62.43으로 가장 낮았다(p<0.05). 육질등급요인 중 육색은 다른 품종들에 비하여 Hereford종이 낮았으며, 지방색은 Angus종이 가장 높았다(p<0.05). 성숙도는 다른 품종에 비하여 Angus종이 가장 낮았으며(p<0.05), 근내지방도, 조직감에서는 품종간에 차이가 없었다. 품종별 근내지방도 등급 출현율은 Angus종이 근내지방도 1^++등급 이상이 50%를 차지하여 가장 높았으며, Hereford종이 전두수 1^++등급 미만으로 가장 낮게 나타났다. 품종별 최종 도체등급 출현율에 있어서 육질등급에서는 Angus종이 육질 2등급 출현율이 20%로 가장 좋았으며, Hereford종과 교잡종(Hereford X Angus)은 100% 육질 3등급으로 가장 저조한 성적을 보였다. 육량등급 출현율에 있어서는 Hereford종이 육량 B등급 출현율 30%로 가장 좋은 성적을 보였으나, Angus종과 교잡종(Hereford X Angus)은 100% 육량 C등급으로 가장 저조한 성적을 보였다. 전체적으로 볼 때 교잡종은 전 두수 육질 3등급, 육량 C등급을 보여 육질 및 육량 면에서 가장 저조하였다. 본 연구결과 수입생우(거세우)는 한우거세우에 비하여 육량, 육질 모든 면에서 저조한 결과를 보였다. 그러나 비육시험에서 한우와 동일하게 시험을 하지 못한 면에서는 아쉬운 점이 있었으며, 한우거세우와 정확한 비교를 위해서는 동일조건에서 한우와 수입생우의 비교 시험이 필요하다고 사료된다. This study was conducted to provide the information for the carcass grading properties of imported beef cattles fed in Korea. The cattles from 4 different breed such as Hereford, Angus, Murray Grey, Hereford×Angus were imported and slaughtered at 700㎏(24month). The Yield and Quality grading characteristics for 10 cattles from each breed were evaluated. Carcass weight of Murray Grey was lower than the other breed(P<0.05). The thickness of backfat was the lowest for Hereford(13.7㎜) and followed by Hereford×Angus(17.30㎜), Angus(18.20㎜) and Murray Grey(18.90㎜). Hereford had the highest Yield index(64.63%) and Murray Grey had the lowest Yield index(62.43)(P<0.05). The frequency percentages of marbling degree for Angus having the I over degrees were 50% which was the highest among the breed. In the frequencies of final carcass grades, Hereford produced 30% of B grade and 70% of C grade in Yield grade and the 3 grade produced in Quality grade. All carcasses of Angus were graded as the grade C in Yield grade and 20% carcasses of Angus had the 2 grade and 80% carcasses had the 3 grade in Quality grade. Murray Grey produced 10% of grade B and 90% of grade C in Yield grade and produced 10% of the 2 grade and 90% of the 3 grade in Quality grade. The Hereford×Angus produced 100% of grade C in Yield grade and 100% of the 3 grade in Quality grade.

Enhanced antitumor immunotherapeutic effect of B-cell-based vaccine transduced with modified adenoviral vector containing type 35 fiber structures

Kim, E-K,Seo, H-S,Chae, M-J,Jeon, I-S,Song, B-Y,Park, Y-J,Ahn, H M,Yun, C-O,Kang, C-Y Macmillan Publishers Limited 2014 Gene therapy Vol.21 No.1

For successful clinical tumor immunotherapy outcomes, strong immune responses against tumor antigens must be generated. Cell-based vaccines compromise one strategy with which to induce appropriate strong immune responses. Previously, we established a natural killer T-cell (NKT) ligand-loaded, adenoviral vector-transduced B-cell-based anticancer cellular vaccine. To enhance tumor antigen delivery to B cells, we established a modified adenoviral vector (Ad-k35) that encoded a truncated form of the breast cancer antigen Her2/neu (Ad-k35HM) in which fiber structure was substituted with adenovirus serotype 35. We observed increased tumor antigen expression with Ad-k35HM in both human and murine B cells. In addition, an Ad-k35HM-transduced B-cell vaccine elicited strong antigen-specific cellular and humoral immune responses that were further enhanced with the additional loading of soluble NKT ligand KBC009. An Ad-k35HM-transduced, KBC009-loaded B-cell vaccine efficiently suppressed the in vivo growth of established tumors in a mouse model. Moreover, the vaccine elicited human leukocyte antigen (HLA)-A2 epitope-specific cytotoxic T-cell responses in B6.Cg (CB)-Tg (HLA-A/H2-D) 2Enge/Jat mice. These findings indicated that the Ad-k35 could be appropriate for the preclinical and clinical development of B-cell-based anticancer immunotherapies.

단감 '부유' 과실의 저장특성에 미치는 수확기 동상해 및 과수원 온도조건의 영향

곽용범,김승희,안광환,이창훈,강성구,Kwack, Y.B.,Kim, S.H.,Ahn, K.H.,Lee, C.H.,Kang, S.K. 국립한국농수산대학교 교육개발센터 2021 현장농업연구지 = Journal of practical agricultural resear Vol.23 No.1

단감 '부유'의 가을 동상해 발생과 과실생장에 미치는 식재위치의 표고와 농장의 온도환경의 영향과, 서리가 발생한 과수원의 과실 저장 특성을 알아보고자 경남 창원(과원 A)과 창녕(과원 B)의 경사지 과원에서 표고별(상, 중, 하)로 과실특성을 조사하고 서리가 발생한 후 수확한 과실을 MA저장 후 저장특성(품질, 저온장해)을 조사하였다. 수확기 전후(10월25일, 11월5일, 11월20일)에 조사된 두 과원의 과실의 생장 특성(과중, 당도, 경도)은 표고와 농장 요인의 유의미한 영향을 받지 않았다. 과실 착색은 표고 요인의 영향을 받았으며, 과실의 착색(hunter 'a')은 B 과원보다 A 과원에서 더 빨리 진행되었다. 수확기에 발생한 저온으로 인하여 과원 B의 하부에서만 약 2%의 과실에서 동상해 피해가 관찰되었다. 두 과원(A, B)에서 11월 5일과 11월 20일에 수확한 과실을 저밀도 폴리에틸렌 필름을 사용하여 MA저장 한 결과, 저온지 과원(B)보다 고온지 과원(A)의 과실 경도가 높게 유지되었다. 그리고 11월 5일에 수확한 과실은 약 80일 저장 후에도 건전과율이 과원 A에서 약 73%, 과원 B에서 약 85%로 높게 유지되었으나, 11월 20일(서리 발생 후) 수확한 과실은 A 과원의 과실은 약 80일 저장 후에도 76%의 높은 건전과율을 유지하였으나 B 과원의 과실 건전과율은 약 14%에 불과했다. We analyzed the effect of planting site (elevation: high, middle, low) and temperature condition of orchard on the frost injury occurrence and storage characteristics of persimmon (Diospyros kaki cv. 'Fuyu) fruits under an early fall frost at two orchards, Changwon (A) and Changnyeong (B), Gyeongsangnam-do Korea. Also, the fruits picked at Nov. 5 and Nov. 20 were MA stored to investigate the quality and chilling injury of fruits. On 2-way factor analysis, both planting elevation and orchard factor had not a significant effect on fruit characteristics (weight, firmness, soluble solid), while planting elevation factor did on the skin color (hunter 'a')(p<0.05). A fruit skin coloration at orchard A was faster than that at orchard B. At low elevation of orchard B, 2% fruits were frost injured at harvest season. On LDPE film MA storage with the fruits harvested at Nov. 5 and Nov. 20, the fruits at orchard A had higher fruit firmness than those of orchard B during storage. In addition, the 73% (orchard A) and 85%(orchard B) fruits harvested at Nov. 5 showed no chilling injury 80 days after storage. However, At harvest of Nov, 20 (after incidence of frost), only the 14% fruits of orchard B had no the chilling disorder 80 days after storage, even though 76% fruits at orchard A did.

Gut commensal Bacteroides acidifaciens prevents obesity and improves insulin sensitivity in mice

Yang, J-Y,Lee, Y-S,Kim, Y,Lee, S-H,Ryu, S,Fukuda, S,Hase, K,Yang, C-S,Lim, H S,Kim, M-S,Kim, H-M,Ahn, S-H,Kwon, B-E,Ko, H-J,Kweon, M-N Society for Mucosal Immunology 2017 Mucosal immunology Vol.10 No.1

<P>In humans, the composition of gut commensal bacteria is closely correlated with obesity. The bacteria modulate metabolites and influence host immunity. In this study, we attempted to determine whether there is a direct correlation between specific commensal bacteria and host metabolism. As mice aged, we found significantly reduced body weight and fat mass in Atg7(Delta CD11c) mice when compared with Atg7(f/f) mice. When mice shared commensal bacteria by cohousing or feces transfer experiments, body weight and fat mass were similar in both mouse groups. By pyrosequencing analysis, Bacteroides acidifaciens (BA) was significantly increased in feces of Atg7(Delta CD11c) mice compared with those of control Atg7(f/f) mice. Wild-type C57BL/6 (B6) mice fed with BA were significantly more likely to gain less weight and fat mass than mice fed with PBS. Of note, the expression level of peroxisome proliferator-activated receptor alpha (PPARa) was consistently increased in the adipose tissues of Atg7(Delta CD11c) mice, B6 mice transferred with fecal microbiota of Atg7(Delta CD11c) mice, and BA-fed B6 mice. Furthermore, B6 mice fed with BA showed elevated insulin levels in serum, accompanied by increased serum glucagon-like peptide-1 and decreased intestinal dipeptidyl peptidase-4. These finding suggest that BA may have potential for treatment of metabolic diseases such as diabetes and obesity.</P>

Induction of Nrf2-mediated phase II detoxifying/antioxidant enzymes in vitro by chitosan-caffeic acid against hydrogen peroxide-induced hepatotoxicity through JNK/ERK pathway

Ahn, C. B.,Je, J. Y.,Kim, Y. S.,Park, S. J.,Kim, B. I. Springer Science + Business Media 2017 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.424 No.1

<P>Chemical modification of chitosan is a promising method for the improvement of biological activity. In this study, chitosan-caffeic acid (CCA) was prepared and its in vitro hepatoprotective ability against hydrogen peroxide-induced hepatic damage in liver cells was evaluated. Treatment with CCA (50-400 A mu g/mL) did not show cytotoxicity and also significantly (p < 0.05) recovered cell viability against 650 A mu M hydrogen peroxide-induced hepatotoxicity. CCA treatment attenuated reactive oxygen species generation and lipid peroxidation in addition to increasing cellular glutathione level in cultured hepatocytes. To validate the underlying mechanism, antioxidant and phase II detoxifying enzyme expressions, which are mediated by NF-E2-related factor 2 (Nrf2) activation, were analyzed and CCA treatment was found to increase the expression of superoxide dismutase-1 (SOD-1), glutathione reductase (GR), heme oxygenase-1 (HO-1), and NAD(P)H:quinine oxidoreductase 1 (NQO1). CCA treatment resulted in increased Nrf2 nuclear translocation. The phosphorylation of extracellular regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) by CCA treatment contributed to Nrf2 activation. Pharmacological blockade of ERK, JNK, and p38 MAPK revealed that SP600125 (JNK inhibitor) and PD98059 (ERK inhibitor) treatment reduced Nrf2 translocation into the nucleus while SB203580 (p38 inhibitor) exhibited weak inhibition. Collectively, CCA protects liver cells against hydrogen peroxide-induced injury and this ability is attributed to the induction of antioxidants and phase II detoxifying enzymes that are mediated by Nrf2 translocation via JNK/ERK signaling.</P>

완전이식형 인공심장 구동을 위한 무선에너지 전송시스템의 개발

안재목,이우철,김희찬,민병구 대한전자공학회 1992 秋季綜合學術大會 論文集 Vol.14 No.2

In systems in which inductive coupling between a pancake-shaped coil on the surface of the body and a similar coil within the body is utilized for the transfort of electromagnetic energy, the minimization of temperature rise in the tissue is intimately related to the achievement of minimum losses in the region of the implanted coil. the new class of amplifiers, named "class E", for inverter is defined and is illustrated by a detailed description and a set of design equations for one single member of the class. For TET circuit the authors measured 65 to 76 percent efficiency at 198khz at 30 to 50 w output from IRF250 MOSFET transistor.

Stabilized ordered-mesoporous Co₃O₄ structures using Al pillar for the superior CO hydrogenation activity to hydrocarbons

Ahn, C.I.,Koo, H.M.,Jo, J.M.,Roh, H.S.,Lee, J.B.,Lee, Y.J.,Jang, E.J.,Bae, J.W. Elsevier BV 2016 Applied Catalysis B Vol. No.

<P>The metal oxide pillared ordered-mesoporous Co3O4 was investigated to design a stable and superior catalyst for CO hydrogenation into linear hydrocarbons through Fischer Tropsch Synthesis (FTS) reaction. Enhanced structural stability was observed in ordered-mesoporous Co3O4 even under hydrogen-excess conditions after modification with a metal oxide pillar of Al2O3. The mesoporous Co3O4 was synthesized using a hard template of highly ordered three dimensional mesoporous KIT-6. A small number of metal oxide pillars such as Al2O3, Mn2O4, and SiO2 with 5 wt% were subsequently added to the orderedmesoporous Co3O4 through the incipient wetness impregnation method. The Al2O3-modifed mesoporous Co3O4 catalyst demonstrated superior CO conversion with a stable activity in CO hydrogenation reaction. The enhanced catalytic stability seems to be attributable to the lower mobility of the Al2O3 pillar which formed stronger interactions with the mesoporous Co3O4 inner surfaces. The Al2O3 modification can effectively stabilize ordered-mesoporous structures of Co3O4 by acting as an ordered mesoporous channel reactor and enhancing the transport rate of hydrocarbons formed during FTS reaction. (C) 2015 Elsevier B.V. All rights reserved.</P>

B-WLL에서 최적화된 경쟁 슬롯 수를 결정하는 동적 무선 자원 할당 알고리즘

박병주(B. J. Park),안계현(K. H. Ahn),고재욱(J. W. Ko),김영천(Y. C. Kim) 한국통신학회 2001 한국통신학회 학술대회 및 강연회 Vol.2001 No.4