우리나라의 축산분뇨 발생 및 처리 현황

전병관,나성주,김선화 동신대학교 환경연구소 2004 환경연구 Vol.9 No.1

우리나라에서 사육되는 주요 가축들을 대상으로 하여 2000년부터 2003년까지 4년 동안 주요통계자료를 바탕으로 가축의 사육 두수와 배출량, 처리시설 현황 등을 분석한 결과 축산분뇨 발생은 대부분의 가축에서 2002년 이후 소폭 증가하였다. 2003년 기준으로 우리나라의 축산분뇨 공공처리 시설은 47개소이며 자원화가 가능한 처리시설은 20개소로써 이는 전체 처리시설의 42.6%밖에 되지 않는 실정이다. 따라서 자원화를 위한 신규 공공처리시설의 확충이나 기존 공공처리시설의 보강이 필요할 것으로 판단된다. 개별 축산시설의 경우 신규축산 농가와 사육두수를 늘리는 축산농가에 대해서는 반드시 분뇨분리 시설을 설치하도록 유도함으로써 점차적으로 분뇨분리 형태의 축사시설을 정착화 시켜야하며, 지역현황이 고려된 적정 축산두수의 유지와 소규모 축산농가들의 계획된 축산단지로 이전 또는 공동시설화해야 할 것이다. After, based on the major statistical data from year 2000 to 2003, the number of domestic livestock-breeding, the status of treatment plants, and the amount of livestock's waste generation had been analyzed, the amount of livestock's excretions generated were increased within narrow limits. At the basic year of 2003, the public treatment plant of livestock's excretions in Korea were 47 places and among them, only 20 plants(42.6%) could make excretions into resources. Therefore, an expansion of new public treatment plants and reinforcement of the existing plants must be needed. At the individual breeding facility, new stock farmhouse and farmhouse who expands the number of livestock must be induced to set up the livestock's waste treatment plant. Hence, gradationally, livestock's waste treatment plants should be installed widely, and the proper number of the livestock should be maintained under the considered status of an area. Also, Small scale stock farmhouses should move to planned complex and collaborate.

폐암에서 CYFRA 21-1과 다른 종양표지자의 진단적 의의

이상구,이호현,전병철,김성자,이영현,김문연,하경임 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

폐암의 확실한 조직학적 진단이 어려울 경우 종양표지자의 검사가 보조적인 진단 수단이 되고, 조기 진단이나 경과 관찰에 이용되고 있다. 이에 저자는 CYFRA 21-1이 폐암의 종양표지자로서 효용성이 있는지 판정하고, CYFRA 21-1과 다른 폐암 종양표지자인 SCC Ag, CEA, NSE의 폐암의 조직학적 유형에 따른 민감도와 특이도를 비교하였다. 그리고 병기 진행에 따른 CYFRA 21-1치의 증가 여부를 관찰하고, 4가지 종양표지자를 동시에 측정하였을 때의 진단적 효용성을 비교하고자 하였다. 이 연구는 1994년 12월부터 1995년 11월까지 동국대학병원에 입원한 폐암 환자 40명과 양성 폐질환 환자 40명을 대상으로 하였다. 편평상피세포암 21명, 선암 10명, 소세포암 7명, 대세포암 2명이었다. 혈청 CYFRA 21-1의 cytokeratin 19 분절에 대한 쥐의 두 가지 단일 클론항체(KS 19-1과 BM 19-21)를 이용하는 RIA방법으로 측정하였다. CEA는 MEIA 방법으로, SCC 항원과 NSE는 RIA로 측정 하였다. 1. CYFRA 21-1의 혈중 농도는 폐암군 22.08±43.00ng/mL, 양성 폐질환군 1.14±1.04ng/mL로 폐암군에서 양성 폐질환군보다 유의하게 높았다(P<0.05). 폐암환자군에서 55%의 양성율을 보였고, SCC 항원 30%, CEA 44.7%, NSE 54.5%의 양성율을 나타내 CYFRA 21-1이 가장 높은 양성율을 보였다. 2.CYFRA 21-1은 비소세포암군 23.79±44.49ng/mL, 소세포암군 13.90±33.57ng/mL로 양군에서 유의한 차이를 보였다. 폐암환자군에서 민감도와 특이도는 CYFRA 21-1 55.0%와 96.7%, SCC항원 30.0%와 96.4%, CEA 42.5%와 92.8%, NSE 36.4%, 76.9%로 CYFRA 21-1에서 가장 높은 민감도와 특이도를 보였다. 폐암의 조직학적 유형에 따른 민감도는 편평상피세포암에서 CYFRA 21-1이 61.9%로 CYFRA 21-1의 민감도가 가장 높았고, 선암에서는 CEA가 88.8%로, 소세포암에서는 NSE가 85.7%로 가장 민감도가 높은 것으로 나타났다. 3. 비소세포암군에서 CYFRA 21-1치는 병기가 진행됨에 따라 증가하는 경향을 보였으나 통계학적으로 유의하지는 않았다. 4. 폐암환자에서 단일 종양표지자를 측정하는 것 보다 동시에 여러 종양표지자를 측정하는 것이 특이도는 떨어지나 민감도는 80.0%로 더 높은 것으로 나타났으며 정확도에는 큰 차이가 없었다. 양성 표지나 수에 따른 비교분석시 폐암의 상대 예측도는 두 표지자 양성인 경우가 76.5%, 3가지 표지자와 4가지 모든 표지자에서 양성으로 나온 경우는 100%로 나타났다. 본 연구 결과 CYFRA 21-1은 새로운 폐암의 종양표지자로 기존의 종양표지자 보다 민감도가 높고 비소세포암 특히, 편평상피세포암에 유용한 표지자로 사료되며, 폐암이 의심되는 환자에서 단일 종양 표지자를 측정하는 것 보다 수종의 종양표지자를 동시에 측정하는 것이 진단에 더욱 도움이 되리라 생각된다. Background: Cytokeratin 19 is a subunit of cytokeratin intermediate filament expressed in simple epithelia and their malignant counterparts. A fragment of cytokeratin subunit 19 can be measured in serum with a immunoradiometric assay using two mouse MoAb KS 19-1 and BM 19-21. Thus this cytokeratin 19 fragment is referred to as CYFRA 21-1. The aims of this study are to evaluate the clinical utility of CYFRA 21-1 in the diagnosis of lung cancer and to compare the diagnostic sensitivity and specificity of CYFRA 21-1 with those of CEA, SCC Ag, NSE according to histological type of lung cancer. Methods: In 40 patients with lung cancer(21 with squamous cell carcinoma, 10 with adenocarcinoma, 7 with small cell carcinoma, 2 with large cell carcinoma) and 40 patients with non-malignant lung disease, serum CYFRA 21-1 was measured by solid-phase immunoradiometric assay(CIS Bio International, France). Serum NSE and SCC Ag were measured by immunoradiometric assay, and CEA was measured by microparticle enzyme immunoassay. Results: 1) The mean value of CYFRA 21-1 was 22.08±43.00ng/mL in the lung cancer and 1.11±1.04ng/mL in me non-malignant lung disease group(P<O.O5). 2) Using the cut-off value of 3.3ng/mL, the sensitivity and specificity of CYFRA 21-1 were 55.0%, 96.7% in the lung cancer. The sensitivity of CYFRA 21.1 was 61.9% in squamous cell carcinoma 3) The level of CYFRA 21-1 was increasing tendency with the progression of stage in non-small cell carcinoma but statistically not significant. 4) Simultaneous determination of four tumor markers revealed increased sensitivity to 50.0% in lung cancer. As the number of positive markers was increased, the relative possibility of lung cancer was also increased. If two markers were positive, it increased to 76.5% and three markers were positive, it increased to 100%.5 Conclusions: CYFRA 21-1 is a useful serum marker for patients with lung cancer, especially in squamous cell carcinoma of the lung. The simultaneous measurement of CYFRA 21-1, CEA. SCC Ag and NSE would provide additional information for the diagnosis of lung cancer, especially in patients with high risk group of lung cancer.

Paraquat 투여 백서에서 Cyclophosphamide와 Dexamethasone의 치료 효과

신정철,신양수,유병전,임현성,정태흥,박찬국,오금탁,이병래 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

Paraquat (N,N'-dimethy 1-4,4'bipyrydinium : Gramoxone^(R), a widely used herbicide, is extremely toxic to all green plants and many eukaryotic organisms. In human, paraquat intoxication leads to multiple organ failure, it preferentially damages the lung, kidney and liver and may result in death. The participation of superoxide in initiating tissue damage by administration of paraquat is well known in which one electron reduction of paraquat leads to the formation of radical species which react with molecular oxygen to give superoxide. This process initiates immunological changes with the activation of neutrophilic leukocytes, which is related to further production of superoxide. In this experiment, therapeutic effects of cyclophosphamide and dexamethasone on blood cell count, malondialdehyde as a indicator of lipid peroxidation, and antioxidant enzymes levels in the lung, liver and kidney of paraquat intoxicated rats were inbestigated. The WBC count were significantly decreased by combined treatment of cyclophosphamide and dexamethasone in paraquat intoxicated rats at 3 and 5 days but RBC count were not changed. The contents of malondialdehyde were significantly decreased in the lung and Kidney by combined treatment at 10 days after paraquat intoxication. The catalase acitvities were markedly increased in the lung and liver of rats at 10 days after paraquat intoxication, and superoxide dismutase activities showed minimal changes in the lung, liver and kidney of rats by combined treatment. These results suggest that paraquat induced tissue damage in rats can be redeced by combined administration of cyclophosphamide and dexamethasone.

표고버섯 경영 표준진단표의 개발 및 현지 적용

전준헌 ( Jun Heon Jeon ),원현규 ( Hyun Kyu Won ),유병일 ( Byoung Il Yoo ),이성연 ( Seong Youn Lee ),이정민 ( Jung Min Lee ),지동현 ( Dong Hyun Ji ),김연태 ( Yeon Tae Kim ),강길남 ( Kil Nam Kang ),오득실 ( Duk Sil Oh ) 한국산림과학회 2013 한국산림과학회지 Vol.102 No.2

This study aims to develop a standard diagnostic table for management and administration that oak mushroom cultivators of forestry households can utilize. By diagnosing their current level of management with the table, the cultivators themselves will be able to grasp and address their operational challenges better. The table is composed of; questions on the status of forestry households; columns for a series of management performance indices, and; questionnaire with 4 categories and 20 subcategories to check the current level of management and administration by the households. In order to prepare the standard diagnostic table for oak mushroom management, 196 forestry households throughout 10areas-Cheong-yang, Gong-ju, Bu-yeo and Seo-cheon in Chuncheongnam-do, Mun-kyung and Ye-cheon in Gyeongsangbuk-do, Jin-an in Jeollabuk-do, Jang-heung and Jang-seong in Jeollanam-do, and Jeju Islands-were interviewed with and a total of 190 questionnaires were acquired and made use of in the result analysis. Then, the score on management level of each forestry household was determined in the way of aggregating scores from each subcategory. The overall average score of every household was calculated at 62.2 point with more than half of the respondents, 54.7%, belonging to the range of 60 to 80. When considered by regional groups, the average score of Jin-an was the lowest with 57.6 point while that of Jang-seong was the highest with 69.6 point. In case of the ‘cultivation management’ category, there were a lot of cultivators who expressed a negative awareness of the term ‘pest control’ because they had a tendency to think the term in connection with ‘herbicides or pesticides’. So it is inevitable to adapt and modify existing groups and grades to make sure that the cultivators can make a right choice without confusing the cincept ‘pest control’ with ‘herbicides or pesticides’. Meanwhile, the average scores of ‘management and administration’ categories were mostly low. It was remarkable in these categories that forestry households in Jeolla province, which had remained in lower ranks in the other three categories, recorded higher scores than those in Chungcheong province, boasting a relatively high level of management and administration.

Cloning and Characterization of the UDP Glucose/Galactose Epimerases of Oryza sativa

( Seong Kyong Kim ),( Dae Hwan Kim ),( Bong Gyu Kim ),( Young Min Jeon ),( Byoung Seok Hong ),( Joong Hoon Ahn ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.4

UDP-glucose/galactose-4-epimerase (UGE) catalyzes the interconversion of UDP-glucose and UDP-galactose. Oryza sativa contains four UGEs. Expression of OsUGE3 was higher than other OsUGEs in all tissues except the stem. OsUGE1 expression was enhanced after drought, salt or UV-irradiation stress. Four rice UGEs (OsUGE1-4) were cloned by polymerase chain reaction and sequenced. OsUGE1-4 were expressed in Escherichia coil with glutathione S-transferase fusion protein and purified. All four OsUGEs could covert UDP-glucose into UDP-galactose better than vice versa. Kinetic parameters of OsUGEs showed that OsUGE1 was most efficient. These OsUGEs could be used for biosynthesis of various UDP-sugars, which serve as a cosubstrates of UDP-dependent glycosyltransferase modification reactions of antibiotics or flavonoids.

Optimization of hexanoic acid production in recombinant Escherichia coli by precise flux rebalancing

Seong Gyeong KIM,Sungho JANG,Jae Hyung LIM,Byoung Seung JEON,Byoung-In SANG,Gyoo Yeol JUNG 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.10

Fine-tuning of atoB expression for optimization of hexanoic acid production in recombinant Escherichia coli

Seong Gyeong KIM,Sungho JANG,Jae Hyung LIM,Byoung Seung JEON,Jungyeong KIM,Kyoung Heon KIM,Byoung-In SANG,Gyoo Yeol JUNG 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

HLA and Insulin-Dependent Diabetes Mellitus in Koreans

Byoung Doo Rhee,Sang Jeon Choi,Sung Woo Park,Dong Seop Choi,Hoon Han,Geum Ryoung Kim,Seong Yeon Kim,Bo Youn Cho,Hong Kyu Lee,Chang Soon Koh,Hun Ki Min 대한내과학회 1987 The Korean Journal of Internal Medicine Vol.2 No.2

Phenotypic characterization of Hanwoo (native Korean cattle) cloned from somatic cells of a single adult

( Byoung Chul Yang ),( Seung Hwan Lee ),( Seong Soo Hwang ),( Hwi Cheul Lee ),( Gi Sun Im ),( Dong Hoon Kim ),( Dong Kyeong Lee ),( Kyung Tai Lee ),( Ik Soo Jeon ),( Sung Jong Oh ),( Soo Bong Park ) 생화학분자생물학회 2012 BMB Reports Vol.45 No.1

We investigated phenotypic differences in Hanwoo cattle cloned from somatic cells of a single adult. Ten genetically identical Hanwoo were generated by somatic cell nuclear transfer from a single adult. Weights at birth, growing pattern, horn and noseprint patterns were characterized to investigate phenotypic differences. The weights of clones at 6 and 12 months were slightly heavier than that of the donor. A horn pattern analysis revealed that seven clones had exactly the same horn pattern as the donor cow, whereas three were different. Although similarities such as general appearance can often be used to identify individual cloned animals, no study has characterized noseprint patterns for this end. A noseprint pattern analysis of all surviving clones showed that all eight animals had distinct noseprints. Four were similar to the donor, and the remaining four had more secondary-like characteristics. [BMB reports 2012; 45(1): 38-43]

호염성 세균 Micrococcus sp.가 생산하는 RNase의 특성

전병운,김찬조,오만진,최성현 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.1

The halophile, Micrococcus sp. which produces RNase was isolated from salted and fermented food. The optimum growth condition of the Micrococcus sp. in pH 7.0 of complex medium containing 2M NaCl, and at 35℃. Optimum condition for enzyme production by this strain was when it was grown in the CM medium . containing 2% yeast extract, 1.5% casamino acid and 2M NaCl in the initial pH 8.5 for 2 days. The maximal RNase activity was observed at pH 8.0 and 55℃. The Km value for RNA was determined to be 5㎎/㎖ by Lineweaver-Burk plot. The RNase activity in the absence of NaCl was maximum, but it. was completely lost by adding of 1.25M NaCl and it, was increased above 1.25M to 2.5M NaCl. When 2.5M NaCl was added, the activity of RNase showed 45% of maximum value.