Down-regulation of SENP1 Expression Increases Apoptosis of Burkitt Lymphoma Cells

Huang, Bin-Bin,Gao, Qing-Mei,Liang, Wei,Xiu, Bing,Zhang, Wen-Jun,Liang, Ai-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.

Dynamic Analysis and Design Calculation Methods for Powertrain Mounting Systems

Wen-Bin Shangguan,Zhao Yinyan 한국자동차공학회 2007 International journal of automotive technology Vol.8 No.6

A method for dynamic analysis and design calculation of a Powertrain Mounting System (PMS) including Hydraulic Engine Mounts (HEM) is developed with the aim of controlling powertrain motion and reducing low-frequency vibration in pitch and bounce modes. Here the pitch mode of the powertrain is defined as the mode rotating around the crankshaft of an engine for a transversely mounted powertrain. The powertrain is modeled as a rigid body connected to rigid ground by rubber mounts and/or HEMs. A mount is simplified as a three-dimensional spring with damping elements in its Local Coordinate System (LCS). The relation between force and displacement of each mount in its LCS is usually nonlinear and is simplified as piecewise linear in five ranges in this paper. An equation for estimating displacements of the powertrain center of gravity (C.G.) under static or quasi-static load is developed using Newton’'s second law, and an iterative algorithm is presented to calculate the displacements. Also an equation for analyzing the dynamic response of the powertrain under ground and engine shake excitations is derived using Newton’'s second law. Formulae for calculating reaction forces and displacements at each mount are presented. A generic PMS with four rubber mounts or two rubber mounts and two HEMs are used to validate the dynamic analysis and design calculation methods. Calculated displacements of the powertrain C.G. under static or quasi-static loads show that a powertrain motion can meet the displacement limits by properly selecting the stiffness and coordinates of the tuning points of each mount in its LCS using the calculation methods developed in this paper. Simulation results of the dynamic responses of a powertrain C.G. and the reaction forces at mounts demonstrate that resonance peaks can be reduced effectively with HEMs designed on the basis of the proposed methods.

Differential antioxidation activities in two alfalfa cultivars under chilling stress

Wen-Bin Wang,김윤희,Haeng-Soon Lee,Xi-Ping Deng,Sang-Soo Kwak 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4

To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at 10C. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

Study on Volume Stability of Chemical Foaming Cement Paste

Wen-bin Liu,Xiong Zhang 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.7

The paper is aimed at investigating the mechanism and cause of volume instability of chemical foaming cement paste. Volume stability were studied by introducing ultimate shear stress of the technical parameter. The research shows that the ultimate shear stress should be between 25 Pa and 40 Pa when the paste was mixed for 30 minutes, and the ultimate shear stress reached 75 Pa when the paste was mixed for 2 hours. The paste is not easy to collapse, sink. By force analyzing of the paste, mathematical model of the paste was established. The ultimate shear stress was controlled by water-binder ratio, quick setting agent and foam stabilizer. The result shows that the water-binder ratio is 0.54~0.58, the setting accelerator is 2% of cement content, and the foam stabilizer is 1.6% of cement content, which played an important role in the volume stability of the paste.

Fabrication of Photopolymer Hierarchical Micronanostructures by Coupling Electrospinning and Photolithography for SERS Substrates

Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3

Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.

Vesicular Stomatitis Virus G Glycoprotein and ATRA Enhanced Bystander Killing of Chemoresistant Leukemic Cells by Herpes Simplex Virus Thymidine Kinase/Ganciclovir

( Bin Yin ),( Chen Xi Hu ),( Zheng Chen ),( Wen Jun Zhao ),( Li Rong Wei ),( Yan Wen Zheng ),( Chao He ),( Yan Zeng ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.2

Vesicular Stomatitis Virus G Glycoprotein and ATRA EnhancedBystander Killing of Chemoresistant Leukemic Cells by HerpesSimplex Virus Thymidine Kinase/Ganciclovir

Simulated Annealing Utilization for Intelligent Reflecting Surfaces

Wen-Bin Li,Thu L. N. Nguyen,Yoan Shin 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.6

In this paper, we first formulate the path loss model of an intelligent reflecting surface (IRS) in terms of IRS reflection coefficients, then adopt a simulated annealing algorithm to determine a global maximum point of the path loss. Consequently, we can improve the performance of IRS-assisted communication system by avoiding such position deviations in practice. Simulation results show that an optimal solution can be achieved by implementing the proposed algorithm.

Fault Diagnosis Model Based on Multi-level Information Fusion for CNC Machine Tools

Wen Yan,Tan Ji-wen,Zhan Hong,Sun Xian-bin 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.8

The difficulty of CNC machine tools fault diagnosis is bigger than other general equipments because of the complex structure and the coupling among subsystems. The fault diagnosis model based on multi-level information fusion and hybrid intelligence is studied to improve reliability of fault diagnosis. Information from built-in sensors is used to monitor the status of CNC machine tools. The diagnosis principles of internal parameters-motor current, torque, temperature and following error are analyzed. Internal information and external sensors are two main sources which provide data to diagnosis. In order to detect effective fault signal, features of time domain, frequency domain and time-frequency domain are extracted. All these features constitute the feature set. The features are selected by the method of Kernel Principal Component Analysis (KCPA). Then the sensitive feature set is obtained. The method of multiple classifier fusion based on fuzzy comprehensive evaluation is researched. The determination method of weight based on information entropy is proposed. This diagnosis model has been tested feed system mechanical fault diagnosis of CNC machine tools and the results show which is effective and versatile.

Enhanced conversion efficiency of crystalline Si solar cells via luminescent down-shifting using Ba2SiO4 : Eu2+ phosphor

Wen Bin Hung,Juyn Ye Chen,Kien Wen Sung,Teng Ming Chen 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.3

The colloidal submicron needle-like Ba2SiO4 : Eu2+ phosphor coated on microtextured silicon surface has been used as a luminescent down-shifting (LDS) material for enhancing the power conversion efficiency of solar cells. Under a simulated onesun illumination, upon phosphor coating the solar cells showed an enhancement of the short-circuit current density (Jsc) increased from 34.00 to 34.48 mA/cm2 , meanwhile the power conversion efficiency (PCE) was enhanced from 15.13% to 15.36%. The related underlying mechanism for these enhancements can be attributed to photon down-shifting and light scattering effect through the investigation of PLE spectrum, reflectance, and external quantum efficiency. Our results indicate that the colloidal submicron needle-like Ba2SiO4 : Eu2+ phosphor not only act as LDS centers in the UV region but also serve as an antireflection coating for enhancing the light absorption in the visible regime.

웹 기반 수준진단 시스템을 통한 맞춤형 생산정보화 도입 가이드라인에 관한 연구

Wen-Bin Zhao,이주연(Ju Yeon Lee),노상도(Sang Do Noh),조현제(Hyun Jei Jo),조용주(Yong Ju Jo),최석우(Seog Ou Choi) (사)한국CDE학회 2011 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2011 No.1

제조기업이 생산정보화시스템(MES) 도입 시, 기업별로 생산환경의 특성이 모두 반영된 정보화로 추진되어야 하는 관계로 대기업과는 달리 중소기업에서 도입하는 데에 많은 어려움을 겪고 있다. 일반적으로 국내의 중소형 제조기업에서 MES 를 도입할 때 기업의 생산환경 특성을 반영하는 업종이나 규모, 생산방식 및 주문방식 등 요소를 고려하지 않고 획일적인 전략을 기반으로 도입을 하고 있으며, 이로 인해 도입 후에도 시스템이 제대로 활용되지 못하고 일부의 간단한 기능만을 사용하게 되거나, 이로 인한 사용자의 만족도가 낮아지는 등의 많은 문제점이 발생하고 있다. 본 논문에서는 생산정보화시스템(MES)의 효율적인 도입을 위해 웹 기반의 정보화 수준 진단시스템을 이용한 생산정보화의 보급/확산 전략을 도출한다. 또한 웹 포털 상에서 이해하기 쉽고 간단한 대화형식의 질문을 통해 해당 기업의 수준과 필요를 도출하여 맞춤형 생산정보화시스템(MES) 도입 가이드라인을 제시한다.