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G. Patrick Flanagan,Cory J. Stephanson,Anne M. Stephanson 한국식품영양과학회 2003 Journal of medicinal food Vol.6 No.3
The hydroxyl radical scavenging capacity and efficacy of a novel organosiliceous anionic hydride compound,silica hydride, were quantified by a recently developed method. The method measures a direct relationship between the hy-droxyl radical scavenging capability of the antioxidant compound and the linear decrease in signal from a fluorescent 2-hy-droxyterephthalate product created by reacting an Fe 21 -EDTA complex in the presence of a potential radical scavenger. Afluorescence signal half-inhibition, IC 50, value of 1.4 6 0.1 mM was obtained for silica hydride compounds. The validity ofthe analysis was verified by electron spin resonance spectroscopy, spectrophotometric analysis of NAD1 /NADH ratios, mi-tochondrial membrane potential measurements, and assays of both cytochrome c(Fe31 ) to cytochrome c(Fe21 ) and epineph-rine to adenochrome reductions.
G. Patrick Flanagan,Cory J. Stephanson,Anne M. Stephanson 한국식품영양과학회 2002 Journal of medicinal food Vol.5 No.1
Treatment of Chinese hamster ovary and mouse hybridoma cells with Mega-H™ brand sil-ica hydride, a marketed antioxidant, after photosensitization with singlet oxygen and hy-droxyl/superoxide reactive oxygen species through the use of rose bengal diacetate and mala-chite green resulted in an effective method of reducing free radical activity by more than 96%against singlet oxygen species and more than 86% for hydroxyl and superoxide free radicalswith the dosage recommended by the manufacturer. The analysis used a combinational spec-trafluorometric technique to determine cell viability and cytotoxicity through the mechanismof intracellular esterase activity and plasma membrane integrity. Photosensitized controls nottreated with silica hydride showed less than 1% viability under the same conditions. The re-duction of the introduced fre radicals and singlet oxygen species and the consequent highlevels of cell viability may be the result of effective and efficient antioxidant and radical scav-enging properties of silica hydride.9