http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa.
Yamaguchi, Takahiro,Lee, Dong Yeon,Miyao, Akio,Hirochika, Hikohiko,An, Gynheung,Hirano, Hiro-Yuki American Society of Plant Physiologists 2006 The Plant cell Vol.18 No.1
<P>The C-class MADS box gene AGAMOUS (AG) plays crucial roles in Arabidopsis thaliana development by regulating the organ identity of stamens and carpels, the repression of A-class genes, and floral meristem determinacy. To examine the conservation and diversification of C-class gene function in monocots, we analyzed two C-class genes in rice (Oryza sativa), OSMADS3 and OSMADS58, which may have arisen by gene duplication before divergence of rice and maize (Zea mays). A knockout line of OSMADS3, in which the gene is disrupted by T-DNA insertion, shows homeotic transformation of stamens into lodicules and ectopic development of lodicules in the second whorl near the palea where lodicules do not form in the wild type but carpels develop almost normally. By contrast, RNA-silenced lines of OSMADS58 develop astonishing flowers that reiterate a set of floral organs, including lodicules, stamens, and carpel-like organs, suggesting that determinacy of the floral meristem is severely affected. These results suggest that the two C-class genes have been partially subfunctionalized during rice evolution (i.e., the functions regulated by AG have been partially partitioned into two paralogous genes, OSMADS3 and OSMADS58, which were produced by a recent gene duplication event in plant evolution).</P>
Yoo, Mi-Ae,LEE, Won-Ho,HA, Hye-Yeong,RYU, Jae-Ryeon,YAMAGUCHI, Masamitsu,FUJIKAWA, Kazuo,MATSUKAGE, Akio,KONDO, Sohei,NISHIDA, Yasuyoshi 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-
DNA polymerase β(pol β)cDNA of rat fused to an enhamcer-promoter ergion plus a poly(A) signal sequence of actin 5C gene of Drosophila(abbreviated polβ) was transferred to the Drosophila genome. Three of four constructed transgenic strains possessing transgene polβ on different chromosomes were studied. Levels of the polβ transcript and those of the polymerization activity of polβ were markedly elevated in cultured cells trans fected with polβ-bearing vectors as well as in embryos of the transgenic strains. The popular idea that DNA polymerase β participates in DNA repair was not supported by the observation that a pair of a normal and a polβ strain, and the other pair of a mei-9 mei-41(DNA-repair deficient double mutations) strain and a polβmei-9 mei-41 strain, showed no difference in survial within each pair after treatment with ultraviolet light, methylmethane sulfonate and mitomycin C. The other idea that DNA polymerase β participates in recombination was supported by the findings that spontaneous freauency of recombination, either meiotic or mitotic, is significantly higher in a transgenic polβstrain than in a non-transgenic strain. The enhanced recomdination frequency in the polβ strain may, however, reflect an indirect effect of over-produced polβ prteins on chromosomal stability. Whatever the direct effect of rat polβis, the transgenic polβ flies will be useful for study of the physiological role of polβ and the mechanism of recombination.
Kenji Koneri,Yasuo Hirono,Daisuke Fujimoto,Katsuji Sawai,Mitsuhiro Morikawa,Makoto Murakami,Takanori Goi,Atsushi Iida,Kanji Katayama,Akio Yamaguchi 대한위암학회 2013 Journal of gastric cancer Vol.13 No.1
Alpha-fetoprotein–producing gastric cancer is associated with poor prognosis because of frequent liver and lymph node metastasis. We present a case with synchronous liver metastasis who survived for 5 years. A 69-year-old man with upper abdominal pain was referred to our hospital. Gastrointestinal endoscopy revealed a Borrmann II-like tumor in the lower part of the stomach. Computed tomography revealed a tumor in the left lobe of the liver. Serum alpha-fetoprotein levels were markedly increased. We performed distal gastrectomy after administering oral tegafur/gimeracil/oteracil potassium and administered hepatic intra-arterial cisplatin injection. Liver metastasis showed partial response on computed tomography. Despite left hepatic lobectomy, further metastases to the liver and mediastinal lymph nodes became difficult to control. After sorafenib tosylate administration, stabilization of the disease was observed for 4 months. We conclude that hepatic intra-arterial chemotherapy and oral administration of sorafenib tosylate may potentially improve the prognosis in such cases.
E2F-dependent transcription of the rafproto-oncogene during Drosophila development
Kwon, Eun-Jeong,Oh, Eun-Jin,Kim, Young-Shin,Hirose, Fumiko,Ohno, Katsuhito,Nishida, Yasuyoshi,Matsukage, Akio,Yamaguchi, Masamitsu,Yoo, Mi-Ae 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-
D-raf, a Drosophila homolog of the raf proto-oncogene, has diverse functions throughout development and is transcribed in a wide range of tissues, with high levels of expression in the ovary and in association with rapid proliferation. The expression pattern resembles those of S phase genes, which are regulated by E2F transcription factors. In the 5'-flanking region of D-raf, four sequences (E2F sites 1-4) similar to the E2F recognition sequence were found, one of them (E2F site 3) being recognized efficiently by Drosophila E2F (dE2F) in vitro. Transient luciferase expression assays confirmed activation of the D-raf gene promoter by dE2F/dDP. Expression Eraf-lacZ was greatly reduced in embryos homozygous for the dE2F mutation. These results suggest that dE2F is likely to be an important regulator of D-raf transcription.
Ryu, Jae-Ryeon,Choi, Thae-Yeong,Kwon, Eun-Jeong,Lee, Won-Ho,Nishida, Yasuyoshi,Hayashi, Yuko,Matsukage, Akio,Yamaguchi, Masamitsu,Yoo, Mi-Ae 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-
The DRE/DREF system plays an important role in transcription of DNA replication genes such as those encoding the 180 and 73 kDa subunits of DNA polymeraseαas well as that encoding PCNA. In this study, we found two sequences homologous to DRE(5'-TATCGATA-3')in the 5'-flanking region(-370 to-357 with respect to the transcription initiation site) of the D-raf gene and confirmed transcription activity through gel mobility shift assay, transient CAT assays, and spatial patterns of lacZ expression in transgenic larval tissues carrying D-raf gene is another target of the Zerknu¨llt(Zen)protein with observation of D-raf repression by Zen protein in cultured cells anf its ectopic expression in the dorsal region of the homozygous zen mutant embryo. The evdience of DRE/DREF involvement in regulation of the D-raf gene obtained in this study strongly supports the idea that the DRE/DREF system is responsible for the coordinated regulation of cell proliferation-related genes in Drosophila.