Phenolic Extract of Dialium guineense Pulp Enhances Reactive Oxygen Species Detoxification in Aflatoxin B1 Hepatocarcinogenesis

Abdulwasiu O. Adeleye,Taofeek O. Ajiboye,Ganiyat A. Iliasu,Folakemi A. Abdussalam,Abdulazeez Balogun,Oluwayemisi B. Ojewuyi,Musa T. Yakubu 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.8

This study investigated the effect of Dialium guineense pulp phenolic extract on aflatoxin B1 (AFB1)–induced oxidative imbalance in rat liver. Reactive oxygen species (ROS) scavenging potentials of free and bound phenolic extract of D. guineense (0.2–1.0 mg/mL) were investigated in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion (O2-), hydrogen peroxide (H2O2), hydroxyl radical, and ferric ion reducing system. In the in vivo study, 35 animals were randomized into seven groups of five rats each. Free and bound phenolic extract (1 mg/mL) produced 66.42% and 93.08%, 57.1% and 86.0%, 62.0% and 90.05%, and 60.11% and 72.37% scavenging effect on DPPH radical, O2 - radical, H2O2, and hydroxyl radical, while ferric ion was significantly reduced. An AFB1-mediated decrease in the activities of ROS detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glucose 6 phosphate dehydrogenase) was significantly attenuated (P < .05). AFB1-mediated elevation in the concentrations of oxidative stress biomarkers; malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl, and percentage DNA fragmentation were significantly lowered by D. guineense phenolic extract (P < .05). Overall, the in vitro and in vivo effects suggest that D. guineense phenolic extract elicited ROS scavenging and detoxification potentials, as well as the capability of preventing lipid peroxidation, protein oxidation, and DNA fragmentation.

Efficient synthesis of bio-based activated carbon (AC) for catalytic systems: A green and sustainable approach

Aderemi Timothy Adeleye,Amos Adeleke Akande,Chuks Kenneth Odoh,Monday Philip,Timothy Tizhe Fidelis,Pigweh Isa Amos,Oluwakemi Oluwabunmi Banjoko 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.96 No.-

Tremendous efforts in developing sustainable processes for integrated production of value-addedproducts/chemicals and fuels in biorefineries increase through delicate designs towards sustainability. This review focuses on the synthesis of activated carbon (AC) from renewable precursors and itsutilisation in catalytic systems for a gentle and sustainable approach. Owing to the unique shape andporosity-controlled properties, these carbon materials could offer strong, active phase-supportinteractions, leading to unusual catalytic activities and selectivity in biomass upgrading. Porous carbonshave been developed and used as heterogeneous solid catalysts infine chemical and biofuels synthesis asa sustainable and economical alternative over homogeneous catalytic systems. This review revealed theAC's significance and potential as solid catalysts/supports in renewable feedstocks' valorisation. Theliterature showed that bio-derived activated carbon could be a promising and sustainable solid catalyst orsupport for producing biofuels/ value-added products with appreciable BET surface area (750 m2/g) andtotal pore volume (0.37 cm3/g). However, the surface area and pore volume vary with the treatment/nature of cellulose used as the precursor for AC production. Finally, the utilisation of these renewablefeedstocks/waste streams presents us with the avenues to realise sustainable synthesis through greenprocess and design for a sustainable future.

Studies on Improved Amylases Developed by Protoplast Fusion of Aspergillus species

( Tolulope Modupe Adeleye ),( Sharafadeen Olateju Kareem ),( Bankole Mobolaji Olufunmilayo ),( Olusegun Atanda ),( Michael Bamitale Osho ),( Olawale Dairo ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.1

Improved amylases were developed from protoplast fusants of two amylase-producing Aspergillus species. Twenty regenerated fusants were screened for amylase production using Remazol Brilliant Blue agar. Crude enzyme extracts produced by solid state fermentation of rice bran were assayed for activity. Three variable factors (temperature, pH and enzyme type) were optimized to increase the amylase activity of the parents and selected fusants using rice bran medium and solid state fermentation. Analysis of this optimization was completed using the Central Composite Design (CCD) of the Response Surface Methodology (RSM). Amylase activity assays conducted at room temperature and 80℃ demonstrated that Aspergillus designates, T5 (920.21 U/ml, 966.67 U/ml), T13 (430 U/ml, 1011.11 U/ml) and T14 (500.63 U/ml, 1012.00 U/ml) all exhibited improved function making them the preferred fusants. Amylases produced from these fusants were observed to be active over the entire pH range evaluated in this study. Fusants T5 and T14 demonstrated optimal activity under acidic and alkaline conditions, respectively. Fusants T13 and T14 produced the most amylase at 72 h while parents TA, TC and fusant T5 produced the most amylase after 96 h of incubation. Response surface methodology examinations revealed that the enzyme from fusant T5 was the optimal enzyme demonstrating the highest activity (1055.17 U/ml) at pH 4 and a temperature of 40℃. This enzyme lost activity with further increases in temperature. Starch hydrolysis using fusant T5 gave the highest yield of glucose (1.6158 g/100 ml). The significant activities of the selected fusants at 28 ± 2℃ and 80℃ and the higher sugar yields from cassava starch hydrolysis over their parental strains indicate that it is possible to improve amylase activity using the protoplast fusion technique.

Prompt neutron lifetime calculations for the NIRR-1 reactor

Ibrahim, Yakubu V.,Adeleye, Micheal O.,Njinga, Raymond L.,Odoi, Henry C.,Jonah, Sunday A. Techno-Press 2015 Advances in energy research Vol.3 No.2

Prompt neutron lifetime calculations have been performed for the NIRR-1 reactor HEU and LEU cores using the 1/v insertion and the Adjoint flux weighing methods. Results of calculations obtained for the HEU and LEU cores are respectively $57.3{\pm}0.8$ and $47.5{\pm}0.7$ for the 1/v insertion and $56.9{\pm}0.3$ and $46.3{\pm}0.5$ for the Adjoint flux. There is a good agreement seen between the two methods for both cores. The prompt neutron lifetime was observed to be shorter in the LEU than for the HEU as expected. However, the Adjoint flux weighing method seemed to be the easiest method in calculating the prompt neutron lifetime for NIRR-1.

Polyphenolic compositions and in vitro angiotensin-I-converting enzyme inhibitory properties of common green leafy vegetables: A comparative study

Ganiyu Oboh,Ayodele Jacob Akinyemi,Blessing Adeleye,Sunday Idowu Oyeleye,Opeyemi Babatunde Ogunsuyi,Ayokunle Olubode Ademosun,Adedayo Oluwaseun Ademiluyi,Aline Augusti Boligon 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This study compared the phenolic compositions of common green leafy vegetable extracts from Vernonia amygdalina (VA), Telfairia occidentalis (TO), Talinium triangulare (TT), and Amaranthus hybridus (AH) and their effects on the angiotensin-I-converting enzyme (ACE) and cisplatin-induced malonylaldehyde (MDA) production in an isolated rat kidney homogenate. HPLC confirmed the presence of phenolic compounds in the extracts. Furthermore, all extracts inhibited ACE activity dosedependently; however, the extract from VA exhibited the highest ACE activity while TT exhibited the least. Incubation of the kidney homogenate with 1mM cisplatin caused an increase in MDA production; however, all the extracts inhibited the level of MDA produced. Nevertheless, VA extract exhibited the highest inhibition. These activities of the vegetable extracts could be attributed to their phenolic compositions and may suggest some possible mechanism of the actions. However, VA appeared to be the most potent among the vegetables tested.

Polyphenolic compositions and in vitro angiotensin-I-converting enzyme inhibitory properties of common green leafy vegetables: A comparative study

Oboh, Ganiyu,Akinyemi, Ayodele Jacob,Adeleye, Blessing,Oyeleye, Sunday Idowu,Ogunsuyi, Opeyemi Babatunde,Ademosun, Ayokunle Olubode,Ademiluyi, Adedayo Oluwaseun,Boligon, Aline Augusti 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This study compared the phenolic compositions of common green leafy vegetable extracts from Vernonia amygdalina (VA), Telfairia occidentalis (TO), Talinium triangulare (TT), and Amaranthus hybridus (AH) and their effects on the angiotensin-I-converting enzyme (ACE) and cisplatin-induced malonylaldehyde (MDA) production in an isolated rat kidney homogenate. HPLC confirmed the presence of phenolic compounds in the extracts. Furthermore, all extracts inhibited ACE activity dose-dependently; however, the extract from VA exhibited the highest ACE activity while TT exhibited the least. Incubation of the kidney homogenate with 1 mM cisplatin caused an increase in MDA production; however, all the extracts inhibited the level of MDA produced. Nevertheless, VA extract exhibited the highest inhibition. These activities of the vegetable extracts could be attributed to their phenolic compositions and may suggest some possible mechanism of the actions. However, VA appeared to be the most potent among the vegetables tested.

A planned review on designing of high-performance nanocomposite nanofiltration membranes for pollutants removal from water

Samaneh Bandehali,Fahime Parvizian,Huimin Ruan,Abdolreza Moghadassi,Jiangnan Shen,Alberto Figoli,Adeyemi S. Adeleye,Nidal Hilal,타케시마쯔라,Enrico Drioli,Sayed Mohsen Hosseini 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-

The shortage of clean water sources and increase of demand for fresh water have become as major globalchallenges not only in industries but also inhuman life. Meanwhile, membrane technology has beenextensively noticed due to high separation efficiency, energy-saving and environmentally-friendly characteristic. So, development of new membrane is a vital step in advancing of membrane application inwater-treatment. This paper presents a comprehensive review on development of high-performancenanofiltration membrane based on nanotechnology for wastewater treatment. Application of new nanomaterialshas enabled fabrication of NF-membranes with improved separation properties for pollutantsremoval from water. Recognizing that conventional membranes are not necessarily suitable for industrialapplications and/or process intensifications many attempts were made recently to use of nanomaterialsinto NF-membranes, aiming to overcome trade-off relationship between flux/selectivity and to improvetheir stability. So, development of new structured nanomaterial with desirable properties is one of mostcrucial research topics for membrane researchers. Accordingly, an overview on different methods todesign of advanced nanomaterial presented that allow us to overcome their inherent problems in membranesfabrication such as agglomeration, defects formation, insufficient pores’ tuning, poor active sitesand anti-fouling properties; however, selection of appropriate nanomaterials is a challenge of utmost difficulty. Various types of nanomaterials such as zeolites, carbon based, framework (MOF/ZIF/COF), nanobiopolymersand special nanoparticles such as SFNPs, SFNCs, POSS, ZCPs, and nickel hydroxide nanosheetare presented and their roles in NF-transport are discussed as well as strategies for surface modificationof nanoparticles to promoting their surface charges density. Moreover, fabrication methods of nanoenhanced NF-membranes including layer by layer assembly, hollow fiber spinning, electrospinning ofnanofibers, construction of thin-film nanocomposite besides blending, co-polymerization; cross-linkingand grafting techniques are discussed. A proper view for selection of appropriate nanoparticles in membranefabrication is offered while considering types of pollutants to be removed.

Serotype Distribution and Virulence Profile of Salmonella enterica Serovars Isolated from Food Animals and Humans in Lagos Nigeria

Abraham, Ajayi,Stella, Smith,Ibidunni, Bode-Sojobi,Coulibaly, Kalpy Julien,Funbi, Jolaiya Tolulope,Isaac, Adeleye Adeyemi The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.2

Distribution of Salmonella enterica serovars and their associated virulence determinants is wide-spread among food animals, which are continuously implicated in periodic salmonellosis outbreaks globally. The aim of this study was to determine and evaluate the diversity of five Salmonella serovar virulence genes (invA, pefA, cdtB, spvC and iroN) isolated from food animals and humans. Using standard microbiological techniques, Salmonella spp. were isolated from the feces of humans and three major food animals. Virulence determinants of the isolates were assayed using PCR. Clonal relatedness of the dominant serovar was determined via pulsed-field gel electrophoresis (PFGE) using the restriction enzyme, Xbal. Seventy one Salmonella spp. were isolated and serotyped into 44 serovars. Non-typhoidal Salmonella (NTS; 68) accounted for majority (95.8%) of the Salmonella serovars. Isolates from chicken (34) accounted for 47.9% of all isolates, out of which S. Budapest (14) was predominant (34.8%). However, the dominant S. Budapest serovars showed no genetic relatedness. The invA gene located on SPI-1 was detected in all isolates. Furthermore, 94% of the isolates from sheep harbored the spvC genes. The iroN gene was present in 50%, 100%, 88%, and 91% of isolates from human, chicken, sheep, and cattle, respectively. The pefA gene was detected in 18 isolates from chicken and a single isolate from sheep. Notably, having diverse Salmonella serovars containing plasmid encoded virulence genes circulating the food chain is of public health significance; hence, surveillance is required.

Serotype Distribution and Virulence Profile of Salmonella enterica Serovars Isolated from Food Animals and Humans in Lagos Nigeria

Ajayi Abraham,Smith Stella,Bode-Sojobi Ibidunni,Kalpy Julien Coulibaly,Jolaiya Tolulope Funbi,Adeleye Adeyemi Isaac 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2

Distribution of Salmonella enterica serovars and their associated virulence determinants is wide-spread among food animals, which are continuously implicated in periodic salmonellosis outbreaks globally. The aim of this study was to determine and evaluate the diversity of five Salmonella serovar virulence genes (invA, pefA, cdtB, spvC and iroN) isolated from food animals and humans. Using standard microbiological techniques, Salmonella spp. were isolated from the feces of humans and three major food animals. Virulence determinants of the isolates were assayed using PCR. Clonal relatedness of the dominant serovar was determined via pulsed-field gel electrophoresis (PFGE) using the restriction enzyme, Xbal. Seventy one Salmonella spp. were isolated and serotyped into 44 serovars. Non-typhoidal Salmonella (NTS; 68) accounted for majority (95.8%) of the Salmonella serovars. Isolates from chicken (34) accounted for 47.9% of all isolates, out of which S. Budapest (14) was predominant (34.8%). However, the dominant S. Budapest serovars showed no genetic relatedness. The invA gene located on SPI-1 was detected in all isolates. Furthermore, 94% of the isolates from sheep harbored the spvC genes. The iroN gene was present in 50%, 100%, 88%, and 91% of isolates from human, chicken, sheep, and cattle, respectively. The pefA gene was detected in 18 isolates from chicken and a single isolate from sheep. Notably, having diverse Salmonella serovars containing plasmid encoded virulence genes circulating the food chain is of public health significance; hence, surveillance is required.