조기분만진통 임부와 만삭 전 조기양막파열 임부의 정맥혈장내 산화 스트레스 지표

김윤하 ( Yoon Ha Kim ),노은지 ( Eun Ji Noh ),백주아 ( Ju A Back ),김종운 ( Jong Woon Kim ),송태복 ( Tae Bok Song ) 대한주산의학회 2015 Perinatology Vol.26 No.4

목적: 조기분만진통 및 만삭 전 조기양막파열 임부의 정맥혈장내 산화능과 항산화능의 차이를 비교하고 산화 스트레스가 조기양막파열의 병태생리에 어떤 역할을 하는가를 규명하고자 하였다.방법: 75명의 임부를 대상으로 정맥혈을 채취하고 원심분리 후 혈장을 얻었다. (1) A군, 정상 임부 24명, (2) B군, 조기분만진통 임부 25명, (3) C군, 만삭 전 조기양막파열 임부 26명. thiobarbituric acid 반응을 이용하여 지질과산화물 농도를 측정하였고, 2,4-dinitrophenylhydrazine 방법을 이용하여 단백질의 carbonyl 기 함량을 측정하였으며, oxygen radical absorbance capacity assay (ORAC)로 총 항산화능을 측정하였다. Mann-Whitney U test, Kruskal Wallis test를 이용하여 분석하였다. 결과: B군과 C군의 정맥혈장내 지질과산화 치는 A군에 비해 의미있게 높았으며(B군: 4.59±0.30, C군: 5.40±0.22 vs. A군: 3.90±0.26 nmol/mg protein, P<0.05), C군의 정맥혈장내 지질과산화 치는 B군에 비해 의미있게 높았다. C군의 정맥혈장내 carbonyl기 함량은 A군에 비해 의미있게 높았으며(C군: 5.68±0.42 vs. A군: 5.43±0.41 nmol/mg protein, P<0.05), A군과 B군간의 carbonyl기 함량에는 차이가 없었다. 항산화능에 있어서는 B군과 C군이 A군에 비해 의미있게 낮았으며(B군: 117.91±0.48, C군: 111.68±1.23 vs. A군: 119.14±1.12 mM/mL, P<0.05), C군이 B군에 비해 의미있게 낮았다(P<0.05). 결론: 만삭 전 조기양막파열시 조기분만진통에 비해 정맥혈장에서 산화적 변화가 증가되어 있었으며 항산화능은 감소되어 있었다. 이는 조기양막파열시 산화적 스트레스가 증가되어 있을 가능성을 시사한다. Purpose: This study was performed to investigate the oxidation and antioxidation capacity in the maternalvenous plasma of preterm labor with intact membranes, and premature rupture of membranes (PPROM), andalso to evaluate their roles in the pathophysiology of PPROM. Methods: Seventy five women in the following categories had venous blood retrieved: (1) Group A, normal pregnancy (n=24). (2) Group B, preterm labor with intact membranes (n=25). (3) Group C, preterm premature rupture of membranes (n=26). Malondialdehyde (MDA) levels as a marker of lipid peroxidation by thiobarbituric acid reaction, protein carbonyl content by 2,4-dinitrophenylhydrazine reaction, and total antioxidant capacity by oxygen radical absorbance capacity assay (ORAC) were measured. Mann-Whitney U test, Kruskal-Wallis test were used for statistical analysis. Results: Lipid peroxide levels in the venous plasma of group B and C were significantly higher than those of group A (group B: 4.59±0.30, group C: 5.40±0.22 vs. group A: 3.90±0.26 nmol/mg protein, P<0.05). Lipid peroxide levels in the venous plasma of group C were significantly higher than those of group B (P<0.05). Protein carbonyl levels in the venous plasma of group C were significantly higher than those of group A (group C: 5.68±0.42 vs. group A: 5.43±0.41 nmol/mg protein, P<0.05). There was no significant difference of protein carbonyl levels in the venous plasma between group A and B. ORAC levels in the venous plasma of group B and C were significantly lower than those of group A (group B: 117.90±0.48, group C: 111.68±1.23 vs. group A: 119.14±1.12 mM/mL, P<0.05). ORAC levels in the venous plasma of group C were significantly lower than those of group B (P<0.05). Conclusion: In the blood of the women with preterm premature rupture of membranes, the lipid peroxidation was increased and the antioxidant capacity was decreased compared to women with normal pregnancy and preterm labor with intact membranes. These results suggest that oxidative stress was increased in preterm premature rupture of membranes.

대전 지역에서 급성 A형 간염의 유전자형에 따른 임상 특성 고찰

이영우 ( Young Woo Lee ),양현웅 ( Hyeon Woong Yang ),이진아 ( Jin A Lee ),윤기호 ( Ki Ho Yun ),양성은 ( Seong Eun Yang ),이민지 ( Min Ji Lee ),박세영 ( Se Young Park ),김새희 ( Sae Hee Kim ),이향이 ( Hyang Ie Lee ),이윤정 ( Yun Ju 대한내과학회 2011 대한내과학회지 Vol.80 No.5

Background/Aims: Acute viral hepatitis A is a major health problem in Korea and the influx of genotype IIIA is thought to be one reason. We examined the differences in the clinical characteristics and laboratory findings of genotypes IA and IIIA in Daejeon. Methods: From November 2009 to June 2010, 81 patients positive for IgM anti-HAV were enrolled prospectively. The hepatitis A was genotyped using real-time polymerase chain reaction. The clinical characteristics and laboratory results were compared on the Results: The mean patient age was 32.6±7.4 years. The mean hospitalization was 7.7±2.4 days. The patient occupation varied. Clinically, vomiting and diarrhea were relatively more prevalent in genotype IIIA than in IA. Abdominal pain and skin spots were relatively more prevalent in genotype IA than in IIIA. The hemoglobin, peak aspartate aminotransferase (AST) level, and C-reactive protein were statistically higher in genotype IIIA than in IA. The distributions of the peak AST, alanine aminotransferase (ALT) and total bilirubin values tended to be perched in genotype IIIA than in IA. The international normalized ratio (INR) tended to be slightly prolonged in genotype IIIA than in IA. Conclusions: Recently, genotype IIIA of acute viral hepatitis A has become prevalent in Daejeon. Hepatitis A genotype IIIA probably causes worse laboratory abnormalities than genotype IA.

OGLE-2017-BLG-1049: ANOTHER GIANT PLANET MICROLENSING EVENT

Kim, Yun Hak,Chung, Sun-Ju,Udalski, A.,Bond, Ian A.,Jung, Youn Kil,Gould, Andrew,Albrow, Michael D.,Han, Cheongho,Hwang, Kyu-Ha,Ryu, Yoon-Hyun,Shin, In-Gu,Shvartzvald, Yossi,Yee, Jennifer C.,Zang, Wei The Korean Astronomical Society 2020 Journal of The Korean Astronomical Society Vol.53 No.6

We report the discovery of a giant exoplanet in the microlensing event OGLE-2017-BLG-1049, with a planet-host star mass ratio of q = 9.53 ± 0.39 × 10-3 and a caustic crossing feature in Korea Microlensing Telescope Network (KMTNet) observations. The caustic crossing feature yields an angular Einstein radius of θE = 0.52 ± 0.11 mas. However, the microlens parallax is not measured because the time scale of the event, tE ≃ 29 days, is too short. Thus, we perform a Bayesian analysis to estimate physical quantities of the lens system. We find that the lens system has a star with mass Mh = 0.55+0.36-0.29 M⊙ hosting a giant planet with Mp = 5.53+3.62-2.87 MJup, at a distance of DL = 5.67+1.11-1.52 kpc. The projected star-planet separation is a⊥ = 3.92+1.10-1.32 au. This means that the planet is located beyond the snow line of the host. The relative lens-source proper motion is μrel ~ 7 mas yr-1, thus the lens and source will be separated from each other within 10 years. After this, it will be possible to measure the flux of the host star with 30 meter class telescopes and to determine its mass.

Ferroelectric and Electrical Properties of Lead-Free (K0.44Na0.52Li0.04)(Nb0.86Ta0.10Sb0.04)O3 Thin Films

Ju Sung Kim,Chang Won Ahn,Amir Ullah,Song A. Chae,Ill Won Kim 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.12

The Li, Ta, and Sb-substituted lead-free (K0.44Na0.52Li0.04)(Nb0.86Ta0.10Sb0.04)O3 (KNLNTS) thin films were fabricated on Pt(111)/TiO2/SiO2/Si substrates using the radio frequency (RF) magnetron sputtering method. The KNLNTS thin films were annealed at 750 C for 1 h in an oxygen ambient. The film with a thickness of 350 nm exhibited a typical ferroelectric P − E hysteresis loop without fatigue even after 1010 pulses. The KNLNTS thin film exhibited a relatively low leakage current density of 10−7 A/cm2 even up to an applied electric field of 142 kV/cm. A well-saturated piezoelectric hysteresis loop was obtained with a piezoelectric coefficient d33 of 21 pm/V.

수입 및 국내 유통 식품 중의 Ochratoxin A에 관한 조사연구

이동호,강민철,이선화,정동윤,김재이,김형수,김은정,유병옥,김연주,정순아,서영선,김인복,홍무기 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

곰팡이 독소 중 현재 식품골전에 r'1 등재되어 있는 ochratoxin A의 규제기준 설정에 대한 기초자료로 활용하기 위한 방안의 일찬으로 곡류, 두류, 견과류 및 장류에 있어서 ochratoxin A의 오염정도를 조사하였다. 실헐방법으로 1차 screening은 ochratoxin test t를, 최종확인은 irnrrlunoaffihie· column을 이용한 HPLC 분석법을 사용하였파. 회수율은 수수를 제외하고 모두 80%이상을 나타내었고 최저겊출 한계치는 0.Sppb였다. 총 121건(보리 9잔, 찹쌀 7건, 백미 8건, 조 9건, 수수10건, 서리태 11건, 녹두 3건, 백태 9건, 동분 5건, 팥 7건, 강낳콩 8건, 땅콩 7건, 호두 7건, 혼합장 11건, 청국장 10건)에 대하여 조사한 결과 1차 screeRing에서 39건의 양성반응을 보였으나 HPLC로 최종 확인 결과 모든 시료에서 불검출 되었다

<i>SlPMEI</i>, a pollen-specific gene in tomato

Kim, Woong Bom,Lim, Chan Ju,Jang, Hyun A.,Yi, So Young,Oh, Sang-Keun,Lee, Ha Yeon,Kim, Hyun A.,Park, Youn-Il,Kwon, Suk-Yoon Canadian Science Publishing 2014 Canadian journal of plant science. Revue canadienn Vol.94 No.1

<P> Kim, W. B., Lim, C. J., Jang, H. A., Yi, S. Y., Oh, S.-K., Lee, H. Y., Kim, H. A., Park, Y.-I. and Kwon, S.-Y. 2014. SlPMEI, a pollen-specific gene in tomato. Can. J. Plant Sci. 94: 73-83. Pectin is one of the main components of plant cell walls, and its biosynthesis is controlled by pectin methylesterase (PME). Pectin methylesterase inhibitors (PMEIs) are key regulators of PME. We report here the cloning and characterization of a novel Solanum lycopersicum L. PMEI gene, SlPMEI. RT-PCR studies of leaf, seed, fruit, flower, and flower organs confirmed that SlPMEI is expressed specifically in pollen. Promoter analysis of SlPMEI revealed pollen-specific cis-acting elements (pollen lat52 and g10). In addition, SlPMEI is expressed independently of abiotic stress, pathogen exposure, and growth stage in tomato, and a histochemical analysis of promoter activity revealed pollen-specific expression in both Arabidopsis and tomato. Under the microscope, we observed pollen-specific GUS expression in the stamen of transgenic tomato plant. These results indicate that the promoter of SlPMEI has strong pollen-specific activity, and could therefore be useful for development of industrially and agronomically important transgenic plants. </P>

Calcium Mobilization through L-type Channels in Hepatic Stellate Cell Is Essential for TGF-b-mediated CTGF

( Jonghwa Kim ),( Sohee Kang ),( Soohyun Park ),( Ju-yeon Cho ),( Won Sohn ),( David A. Brenner ),( Yong-han Paik ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: In hepatic fibrogenesis hepatic stellate cell (HSC) is a major cell type responsible for producing a major profibrogenic cytokine TGF-b, and connective tissue growth factor (CTGF), a major fibrogenic mediator in several organs. The multi-functional nature of TGF-b signaling in hepatic fibrogenesis is still elusive. At the previous The Liver Week(2015) we reported that Pyk2 is essential for TGF-b-mediated, Smad-independent CTGF induction. Pyk2 is known to be calcium- sensitive, and TGF-b was reported to increase intracellular calcium level. Therefore, we investigated the relation between intracellular calcium levels and pro-fibrogenic TGF-b/Pyk2 axis in hepatic stellate cell. Methods: Immortalized human stellate cell line, LX-2, has been cultured. After TGF-b treatment, expression of CTGF and a-SMA were assessed with RT-PCR and western blot. Pharmacological inhibitor and siRNA-mediated knockdown were used to modulate the activities and expression levels of protein. Intracelllular calcium mobilization was measured with Fura-4/AM. Activation of Pyk2 was addressed in western blot using different phosphorylation site-specific antibodies. Results: CTGF expression was up-regulated within 1hr in TGF-b stimulated LX-2. This up-regulation was greatly suppressed by siRNA-mediated knockdown and pharmacological inhibitor of Pyk2. TGF-b treatment increased phosphorylation of Pyk2 on tyrosine 402, 579/580, and 881. Consistent with the previous reports, TGF-b increased intracellular calcium concentration in Fura-4-preloaded LX-2. CTGF induction by TGF-b was blocked in dose dependent manner by pre-treatment with BAPTA-AM, an intracellular calcium chelator, while A23187, a calcium ionopore, increased CTGF induction even in the absence of TGF-b, suggesting that increase of intracellular calcium level is enough to induce CTGF expression. In addition, A23187 increased phosphorylation of Pyk2, and CTGF induction by A23187 is also greatly reduced by siRNA of Pyk2. Pre-treament of LX-2 with Nifedipine (an L-type calcium channel blocker) suppressed CTGF induction by TGF-b in dose-dependent manner, while FPL64176 (L-type channel activator) increased CTGF expression without TGF-b. The CTGF up-regulations by TGF-b, A23187, and FPL64176 were all suppressed by siRNA-mediated knockdown and pharmacological inhibitors of Pyk2. Conclusions: In hepatic stellate cell, TGF-b increases the intracellular calcium level through L-type calcium channel, leading to downstream Pyk2 signaling for CTGF induction.

Identification and Characterization of a Bacteriocin from the Newly Isolated Bacillus subtilis HD15 with Inhibitory Effects against Bacillus cereus

홍성욱,Kim Jong-Hui,Cha Hyun A,정건섭,Bae Hyo Ju,Park Won Seo,Ham Jun-Sang,Park Beom-Young,Oh Mi-Hwa 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.11

Natural antimicrobial substances are needed as alternatives to synthetic antimicrobials to protect against foodborne pathogens. In this study, a bacteriocin-producing bacterium, Bacillus subtilis HD15, was isolated from doenjang, a traditional Korean fermented soybean paste. We sequenced the complete genome of B. subtilis HD15. This genome size was 4,173,431 bp with a G + C content of of 43.58%, 4,305 genes, and 4,222 protein-coding genes with predicted functions, including a subtilosin A gene cluster. The bacteriocin was purified by ammonium sulfate precipitation, Diethylaminoethanol-Sepharose chromatography, and Sephacryl gel filtration, with 12.4-fold purification and 26.2% yield, respectively. The purified protein had a molecular weight of 3.6 kDa. The N-terminal amino acid sequence showed the highest similarity to Bacillus subtilis 168 subtilosin A (78%) but only 68% similarity to B. tequilensis subtilosin proteins, indicating that the antimicrobial substance isolated from B. subtilis HD15 is a novel bacteriocin related to subtilosin A. The purified protein from B. subtilis HD15 exhibited high antimicrobial activity against Listeria monocytogenes and Bacillus cereus. It showed stable activity in the range 0–70°C and pH 2–10 and was completely inhibited by protease, proteinase K, and pronase E treatment, suggesting that it is a proteinaceous substance. These findings support the potential industrial applications of the novel bacteriocin purified from B. subtilis HD15.

수정-IPA를 통한 푸드카빙교육 효용성의 품질속성 분류에 관한 연구

이성주(Lee, Sung-Ju),임현철(Lim, Hyun-Cheo),김상미(Kim, Sang-Mi) 한국외식경영학회 2022 외식경영연구 Vol.25 No.2

본 연구는 푸드카빙 교육을 받은 수강생들이 지각하는 푸드카빙 효용성은 어떠한지 살펴보고, 어떠한 효용성들이 있고, 효용성의 품질 속성은 어떠한지 살펴보고자 하였다. 이러한 연구목적을 규명하기 위하여 푸드카빙 교육을 받은 경험이 있는 수강생을 모집단으로 설정하였고, 설문조사는 2020년 7월 20일부터 8월 20일까지 한 달간 진행되었다. 설문지는 총 500부를 배부하였고, 435부가 회수되었고, 불성실한 설문지 38부를 제외하고 397부가 최종 분석에 사용되었다. 분석결과, 중요 실행 요소로는 푸드카빙 교육은 지루하지 않고, 남녀노소 할 수 있고, 잡생각이 사라지고, 자존감이 높아지고, 자신의 가치가 높아지고, 마음이 편해지고, 우울함이 사라지는 등의 수업 자체에서 오는 만족감의 속성들이 포함되었다. 매력적 요소에는 푸드카빙 기술이 수익으로 연결되고, 강좌를 개설하고, 적은 금액으로 공방을 오픈하는 등의 수익 측면과 관련된 항목들이 포함되었다. 아울러, 기본적 요소에는 집중할 수 있고, 기분이 좋아지고, 자격증을 취득하고, 전문가가 될 수 있다는 측면의 항목들이 포함되었다. 이는 대체로 푸드카빙을 처음 배우는 수강생들이 기본적으로 가지는 목적들이 포함되었다. This study aims to examine the food carving utility perceived by students who received food carving education, the types of the utility, and its quality attributes. To investigate this research purpose, students who had received food carving education were set as a population, and the survey was conducted for one month from July 20 through August 20, 2020. 500 copies of the questionnaire were distributed in total, and 435 copies were collected. 397 copies were used in the final analysis, excluding 38 unreliable copies. As a result of the analysis, important execution factors included the attributes of satisfaction with the class itself, e.g., Food carving education was not boring; it could be done by men and women of all ages; it made cockeyed ideas disappear; it promoted their self-esteem; it enhanced their value; it comforted their hearts; and it made their depression disappear. Attractive elements included revenue-related items, such as food carving skills connected to profits; they could open lectures; and they could open a workshop with little money. Along with this, the basic elements included the following: They could concentrate; they could feel better; they could get a certificate; and they could become an expert. They usually included the purposes the students who would learn food carving for the first time had basically.

Novel amyloid precursor protein mutation, Val669Leu (“Seoul <i>APP</i>”), in a Korean patient with early-onset Alzheimer's disease

Bagyinszky, Eva,Kang, Min Ju,Van Giau, Vo,Shim, KyuHwan,Pyun, Jung-Min,Suh, Jeewon,An, Seong Soo A.,Kim, SangYun Elsevier 2019 NEUROBIOLOGY OF AGING Vol.84 No.-

<P><B>Abstract</B></P> <P>In this study, a novel mutation in <I>APP</I> gene, Val669Leu (“Seoul <I>APP</I>”), was reported in a Korean female patient with Alzheimer's disease. She developed cognitive decline at 56 years of age, and her memory declined rapidly over one-year period from her 1st visit to the hospital. Her Mini-Mental State Examination scores dropped from 25/30 to 13/30. Two years later, she developed parkinsonian features, myoclonic jerk, and generalized seizure. As the disease progressed, aggravated diffuse brain atrophy and small-vessel ischemic lesion was also observed, and she became mute and vegetative in 4 years from the symptom onset. Magnetic resonance imaging showed mild medial temporal lobe and hippocampal atrophy, and 18F-fluoro-deoxyglucose positron emission tomography showed bilateral temporoparietal hypometabolism. Plasma amyloid oligomer analysis revealed highly elevated Aβ oligomers levels in the proband patient. Family history revealed positive without biochemical confirmation because family members testified similar type of cognitive decline from the proband's mother and one of her aunt/uncle. Her half-siblings did not present any signs of memory impairment. Sanger sequencing of the proband patient revealed a novel mutation in <I>APP</I> gene, Val669Leu, but mutation was not found in her unaffected half-sisters. A designed algorithm by Guerreiro et al. on early-onset Alzheimer's disease–associated mutations suggested the mutation as possibly pathogenic mutation. On the other hand, PolyPhen2 and SIFT tools suggested as otherwise. Since the mutation was located nearby the β-secretase cleavage site of APP, right next to the Swedish APP (Lys,Met670/671Asn,Leu) mutation, it was named as “Seoul <I>APP”</I> mutation. 3D modeling revealed that this mutation could result in significant changes in loop orientation of APP and also its intramolecular interactions. Hence, a novel <I>APP</I> Val669Leu mutation could alter the binding interactions between APP and β-secretase, which may influence the Aβ40 and Aβ42 generations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel variant in APP, Val669Leu, was found in a Korean patient, named Seoul APP. </LI> <LI> Proband patient developed disease phenotype in her 50s. </LI> <LI> Family history may be positive, and mutation may segregate with disease. </LI> <LI> Mutation is located nearby the beta-secretase site of APP, and potentially disturbs the enzyme mechanism. </LI> </UL> </P>