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Compound K improves skin barrier function by increasing SPINK5 expression
Park, No-June,Bong, Sim-Kyu,Lee, Sullim,Jung, Yujung,Jegal, Hyun,Kim, Jinchul,Kim, Si-Kwan,Kim, Yong Kee,Kim, Su-Nam The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.6
Background: The skin acts as a barrier to protect organisms against harmful exogenous agents. Compound K (CK) is an active metabolite of ginsenoside Rb1, Rb2 and Rc, and researchers have focused on its skin protective efficacy. In this study, we hypothesized that increased expression of the serine protease inhibitor Kazal type-5 (SPINK5) may improve skin barrier function. Methods: We screened several ginsenosides to increase SPINK5 gene promoter activity using a transactivation assay and found that CK can increase SPINK5 expression. To investigate the protective effect of CK on the skin barrier, RT-PCR and Western blotting were performed to investigate the expression levels of SPINK5, kallikrein 5 (KLK5), KLK7 and PAR2 in UVB-irradiated HaCaT cells. Measurement of transepidermal water loss (TEWL) and histological changes associated with the skin barrier were performed in a UVB-irradiated mouse model and a 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis-like model. Results: CK treatment increased the expression of SPINK5 and decreased the expression of its downstream genes, such as KLKs and PAR2. In the UVB-irradiated mouse model and the DNCB-induced atopic dermatitis model, CK restored increased TEWL and decreased hydration and epidermal hyperplasia. In addition, CK normalized the reduced SPINK5 expression caused by UVB or DNCB, thereby restoring the expression of the proteins involved in desquamation to a level similar to normal. Conclusions: Our data showed that CK contributes to improving skin-barrier function in UVB-irradiated and DNCB-induced atopic dermatitis-like models through SPINK5. These results suggest that therapeutic attempts with CK might be useful in treating barrier-disrupted diseases.
전기영동에 의한 황어의 계통발생 단계 및 성체 조질들에서 동위효소의 유형
소준노,류훈,장선일,이금형,박호원 한국유전학회 1989 Genes & Genomics Vol.11 No.2
Developing embryos of dace, Tribolodon hakonensis, were analyzed with regard to both gross morphological changes and specific enzymatic changes from the unfertilized egg stage until some 3 week posthatching. The patterns of gene expression among the different tissues in adults were also investigated. The tissue and developmental expression of 13 loci encoding various enzymes and isozymes were visualized by histochemical staining after polyacrylamide gel electrophoresis. Distribution of enzymes and isozymes in the adult tissues usually show tissue specific patterns. The expression of LDH-C and G6-PD-1 loci was the more restricted than the other loci, and these loci were expressed only predominantly in the liver. Many of the enzyme systems investigated exhibited rich patterns (all loci except for MDH-1A locus) of ontogenetic change, while a few (MDH-1A) remained relatively unchanged throughout the period studied. The appearance of several rather tissue - specific isozymes (LDH-A, C and MDH-1B) was closely correleated with the morphological and functional differentiation of the muscle, liver and gill tissue.
Jin-Gu No,Sung-June Byun,Hyeon Yang,Sun A Ock,Jae-Seok Woo,Hwi-Cheul Lee,In-sul Hwang,Ji-Youn Kim,Sang Hyoun Park,Joo Young Lee,Keon Bong Oh 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3
Acute vascular rejection has been known as a main barrier occurring in a xenograted tissue of alpha 1,3-galactosyltransferase knock-out (GalT KO) pig into a non-human primate (NHP). Adenosine which is a final metabolite following sequential hydrolysis of nucleotide by ecto-nucleotidases such as CD39 and CD73, act as a regulator of coagulation, and inflammation. Thus xenotransplantation of CD39 and CD73 expressing pig under the GalT KO background could lead to enhanced survival of recipient NHP. We constructed a human CD39 and CD73 expression cassette designed for endothelial cell-specific expression using porcine Icam2 promoter (pIcam2-hCD39/hCD73). We performed isolation of endothelial cells (pAEC) from aorta of 4 week-old GalT KO and membrane cofactor protein expressing pig (GalT-MCP/-MCP). We were able to verify that isolated cells were endothelial-like cells using immunofluorescence staining analysis with von Willebrand factor antibody, which is well known as an endothelial maker, and tubal formation assay. To find optimal condition for efficient transfection into pAEC, we performed transfection with GFP expression vector using four programs of nucleofection, M-003, U-023, W-023 and Y-022. We were able find that the program W-023 was optimal for pAEC with regard to viability and transfection efficiency by flow cytometry and fluorescent microscopy analyses. Finally, we were able to obtain GalT-MCP/-MCP/CD39/CD73 pAEC expressing CD39 and CD73 at levels of 33.3% and 26.8%, respectively. We suggested that pACE isolated from GalT-MCP/-MCP pig might be provided as a basic resource to understand biochemical and molecular mechanisms of the rejections and as an alternative donor cells to generate GalT-MCP/-MCP/CD39/CD73 pig expressing CD39 and CD73 at endothelial cells.
박웅(Woong Park),유두만(Du Man Yoo),소준노(June No So) 한국생물공학회 2014 KSBB Journal Vol.29 No.1
Planaria were recently reported to be a simple and sensitive model to investigate the mechanistic aspect of seizure and to screen potential anticonvulsants. Using planarian model, we analyzed the pharmacological effect of ukgansan (UGS), an oriental herbal medicine containing seven medicinal herbs, on the planarian locomotor velocity (pLMV) and glutamate-induced seizure-like activity (pSLA). To test whether D. japonica is suitable for studying anti-seizure agents, we investigated the effect of glutamate on pLMV and pSLA in D. japonica. In the present study we first confirmed that pSLA in D. japonica was induced by L-glutamate. Glutamate significantly produced pSLA in a dose dependent manner, but did not affect pLMV. These glutamate-induced paroxysms were decreased by antiepileptic drug, topiramate. A similar inhibitory effect on glutamate-induced pSLA was observed after the treatment of UGS. The present results suggest that UGS and its active constituents possess useful substance inhibiting seizure in planarian and that D. japonica provides a convenient model to search active herbs containing anti-seizure activity.
Lee, Sullim,Park, No-June,Bong, Sim-Kyu,Jegal, Jonghwan,Park, Sang-a,Kim, Su-Nam,Yang, Min Hye Elsevier 2018 Journal of Ethnopharmacology Vol.214 No.-
<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>The fruits of <I>Juniperus rigida</I> have been used in Korean traditional medicine for the treatment of inflammatory diseases in humans such as rheumatoid arthritis.</P> <P><B>Aim of the study</B></P> <P>This study aimed to investigate the anti-atopic properties of <I>J. rigida</I> fruit in <I>in vivo</I> murine atopic dermatitis (AD) models.</P> <P><B>Methods and results</B></P> <P>BALB/c mouse ears ad SKH-1 hairless mice stimulated with oxazolone (4 weeks) and DNCB (3 weeks), respectively, were treated with the 1% <I>Juniperus rigida</I> fruit EtOH extract (JFE). The JFE improved AD symptoms in both oxazolone- and DNCB-induced AD mice by accelerating skin barrier recovery function and suppressing the overproduction of serum immunoglobulin E (IgE) and interleukin 4 (IL-4). The JFE was found to contain isoscutellarein-7-<I>O</I>-β-xylopyranoside, cupressuflavone, podocarpusflavone A, and hinokiflavone as major components based on phytochemical analysis. Eight flavonoids were isolated from JFE, and of those, cupressuflavone and isoscutellarein-7-<I>O</I>-β-xylopyranoside strongly down-regulated IL-4 expression and β-hexosaminidase release in RBL-2H3 cells.</P> <P><B>Conclusion</B></P> <P>Therapeutic attempts with <I>J. rigida</I> fruit and its active components might be useful in treating AD and related skin inflammatory diseases.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Sang-a Park ),( Sim-kyu Bong ),( Jin Woo Lee ),( No-june Park ),( Yongsoo Choi ),( Sang Moo Kim ),( Min Hye Yang ),( Yong Kee Kim ),( Su-nam Kim ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.6
Naturally derived diosmetin and its glycoside diosmin are known to be effective in treating inflammatory disease. This study was performed to determine whether diosmin and diosmetin have the effect of improving atopic dermatitis in a 2,4-dinitrochlorobenzen (DNCB)-induced atopic dermatitis (AD) model. DNCB was used to establish AD model in hairless mice. Skin moisture, serum immunoglobulin E (IgE), interleukin 4 (IL-4), and histological analysis were performed to measure the effectiveness of diosmin and diosmetine to improve AD. IL-4 levels were also measured in RBL-2H3 cells. Administration of diosmetin or diosmin orally inhibited the progress of DNCB-induced AD-like lesions in murine models by inhibiting transdermal water loss (TEWL) and increasing skin hydration. Diosmetin or diosmin treatment also reduced IgE and IL-4 levels in AD-induced hairless mouse serum samples. However, in the in vitro assay, only diosmetin, not diosmin, reduced the expression level of IL-4 mRNA in RBL-2H3 cells. Diosmin and diosmetine alleviated the altered epidermal thickness and immune cell infiltration in AD. Diosmin is considered effective in the cure of AD and skin inflammatory diseases by being converted into diosmetin in the body by pharmacokinetic metabolism. Thus, oral administration of diosmetin and diosmin might be a useful agent for the treatment of AD and cutaneous inflammatory diseases.
Jo, Beom-Geun,Park, No-June,Jegal, Jonghwan,Choi, Sangho,Lee, Sang,Yi, Li,Kim, Su-Nam,Yang, Min Georg Thieme Verlag KG 2019 Planta medica Vol.85 No.7
<B>Abstract</B><P> Stellera chamaejasme, also known as “Langdu”, has been traditionally used for the management of skin-related diseases such as psoriasis and skin ulcers. The aim of this study was to determine whether S. chamaejasme and its major component, luteolin 7-O-glucoside, have a preventive effect on the development of atopic dermatitis in oxazolone-treated BALB/c mice and 2,4-dinitrochlorobenzene-treated hairless mice. The epicutaneous applications of oxazolone and 2,4-dinitrochlorobenzene evoke an experimental murine atopic dermatitis-like reaction in BALB/c mouse ears and SKH-1 hairless mice. Atopic skin symptoms, including erythema (redness), pruritus (itching), exudation (weeping), excoriation (peeling), and lichenification (skin thickening), responded to treatment with S. chamaejasme aerial parts EtOH extract for 2 or 3 weeks. Histopathological examination revealed S. chamaejasme aerial parts EtOH extract significantly reduced inflammatory cell infiltration when applied to atopic dermatitis mice. In addition, luteolin 7-O-glucoside, the major active compound of the S. chamaejasme aerial parts EtOH extract, decreased serum IgE and IL-4 levels and transepidermal water loss and increased skin hydration, therefore exhibiting strong anti-atopic dermatitis activity in 2,4-dinitrochlorobenzene-induced atopic dermatitis mice. In this study, we confirmed antipruritic and antidermatitic effects of S. chamaejasme extract and its main component luteolin 7-O-glucoside in atopic dermatitis murine models. The study shows S. chamaejasme aerial parts EtOH extract and luteolin 7-O-glucoside are most likely to be potential drug candidates for atopic dermatitis treatment.</P>