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위암주변점막에서의 c-erbB-2, p53 및 PCNA 발현양상 및 의의
이재복,김한겸,현진해,김예회,황정웅,김세민 고려대학교 의과대학 1995 고려대 의대 잡지 Vol.32 No.3
It has been postulated that dysplasia and intestinal metaplasia are precancerous stages of gastric tumorigenesis. It is known that c-erbB-2 and p53 oncoprotein are expressed in some gastric cancer and adjacent epithelia. To define the significance of dysplasia and intestinal metaplasia as a precancerous lesion, the expression rate of c-erbB-2 and p53 oncoprotein were immunohistochemically evaluated and compared with clinicopathologicalcharacteristics and PCNA index in the gastric cancer and adjacent dysplastic, metaplastic and normal mucosa. From the resected stomach of 56 gastric cancer patients who had been operated on July 25 through September 23 of 1992. 56 cases of cancerous tissue, dysplasia 21 cases, intestinal metaplasia 43 cases and normal mucosa 21 cases were selected and evaluated, respectively. The results were summarized as follows: 1. The expression rates of c-erbB-2 protein were 13/56(23.2%) in gastric cancer, 4/21(19.0%) in dysplasia, 2/43(4.6%) in intestinal metaplasia and 0/21 in normal mucosa. (p=0.007) 2. The expression rates of p53 protein were 26/56 (46.4%) in gastric cancer, 4/21 (19.1%) in dysplasia, 0/43 in intestinal metaplasia and 0/21 in normal mucosa. (p=0.000) 3. PCNA labeling indices were 43.9±18.1% in gastric cancer, 35.1±8.2% in dysplasia, 28.0±16.1% in intestinal metaplasia and 12.8±9.6% in normal mucosa. (p=0.000) 4. In the neck and basal portion of gastric glandular structures, PCNA labeling indices of dysplasia and metaplasia were higher than the index of normal mucosa. (p<0.05) 5. The cancerous tissue showed higher c-erbB-2 protein expression rate in the differentiated, intestinal- type of cancer than undifferentiated, diffuse type and the dysplasic rnucosa adjacent to advanced gastric cancer showed higher c-erbB-2 protein expression rate than early gastric cancer. 6. There was no significant correlation between p53 protein expression and clinicopathological variables. 7. Positive expression of c-erbB-2 protein showed high PCNA labeling index in the advanced, diffuse-type and undifferentiated gastric cancer, with lymphovascular emboli but the p53 expression was not closely related to the poor prognostic factors of gastric cancer. The above result showed that c-erbB-2 protein was expressed in the dysplastic and metaplastic gastric mucosa adjacent to the gastric cancer and that p53 protein was expressed in the dysplastic gastric mucosa. In the advenced gastric cancer, positive expression of c-erbB-2 and p53 protein in the dysplastic mucosa showed high proliferating activity. Despite limited cases of dysplastic lesion adjacent to gastric cancer, the dysplastic mucosa should be evaluated to precancerous lesion and the immunohistochemical staining of c-erbB-2 and p53 protein might prove useful in monitoring the evaluation of the disease in the follow-up patients at risk of developing gastric cancer.
배정원,이은숙,조민영,구범환,황정웅,김인선,박설희,이민재 고려대학교 의과대학 1998 고려대 의대 잡지 Vol.35 No.2
Breast cancer presents the third common malignancy in Korean women. The etiology of breast cancer involves very complex factors such as genetic, hormonal, and dietary. The peak age of Korean women's breast cancer is more earlier, about ten years, than western women. Extensive genetic analysis of breast cancer have been done trying to correlate these genetic alterations with the disease. p53 gene is a tumor suppressor gene. Mutations eliminating or altering the p53 protein function are the single most common genetic alteration in nearly all types of human cancers. The product of the p53 gene is hypothesized to maintain genomic stability by blocking cell replication or by initiating apoptosis after DNA damage. This study investigated to determine p53 gene mutations detected at the DNA level. Abnormalities of the p53 genes were examined in 27 primary freshed breast cancer tissues. Mutations in p53 exons 5-7 were identified in 2 of 27 cases(7%) using a polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP) analysis: one is frameshift mutation which is the addition of c(gcc→Cgcc) at codon 159, exon 5, the other is missense mutation which substitutes tcc to tGc at codon 241, exon 7. In conclusion, mutant biology of p53 gene in Korean women's breast cancer is missense and frameshift. It is necessary to perform further study in mutation of other exons 2, 8, 9, and 11 of p53 gene.