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미세조류 Chlamydomonas reinhardtii dZL 균주의 100 L 평판형광생물 반응기를 이용한 대량 배양에서의 접종 농도에 따른 바이오매스 생산성 향상 연구
박한울(Hanwool Park),강성모(Sung-Mo Kang),민지호(Ji-Ho Min),김준호(Jun-Ho Kim),조용희(Yonghee Cho),김기현(Ki-Hyun Kim),진언선(Eonseon Jin),홍성주(Seong-Joo Hong),이철균(Choul-Gyun Lee) 한국생물공학회 2020 KSBB Journal Vol.35 No.1
Beside the commercialized pigments from microalgae, such as astaxanthin, beta-carotene, and c-phycocyanin, zeaxanthin is another pigment with great potential for commercialization as it can be used to prevent or cure age-related macular degeneration. However, low zeaxanthin productivity due to its low content in microalgal biomass has been the major obstacle to commercialization. With recent advances in genome editing technology, a green microalga Chlamydomonas reinhardtii dZL was engineered to accumulate zeaxanthin with growth by knocking out enzymes that convert zeaxanthin into other carotenoids. In the present study, C. reinhardtii dZL was cultivated using TAP medium in 100 L flat-panel photobioreactors (PBRs), and the effect of initial cell density(ICD) on biomass productivity in batch operation was investigated. ICD of 0.1 g/L was predicted to yield highest biomass productivity from experiments using 2 L bubble column PBRs. In 100 L flat-panel PBRs, as ICD increased from 0.005 g/L to 0.08 g/L, biomass productivity was increased from 0.03 g/L/ day to 0.16 g/L/day. However, further increasing ICD from 0.08 g/L to 0.16 g/L reduced to 0.14 g/L/day as the maximum biomass concentration was limited by the light intensity. The results showed that ICD could affect biomass productivity in positive and negative ways, and thus ICD should carefully be determined with regards to the culture conditions. Such strategy could be applied to improve productivity for other compounds of interests in other microalgae as well.
저온 생장성이 우수한 분리 미세조류 Tetraselmis sp. 5개주의 생장 패턴 및 지방산 조성 분석
박한울(Hanwool Park),허동희(Donghee Hoh),신동우(Dong-Woo Shin),김지훈(Z-Hun Kim),홍성주(Seong-Joo Hong),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2019 한국해양바이오학회지 Vol.11 No.1
For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could improve overall productivity. Additionally, use of indigenous strain is preferred to alleviate potential impacts on the environment. In this study, five strains of Tetraselmis sp. from nearshore of Youngheung Island, Incheon, Korea, were isolated during winter and characterized for their growth patterns and fatty acid compositions in the low temperatures (5–15°C). The five strains showed various characteristics in optimal growth temperature, fatty acid contents, and compositions. Compared with a strain of Tetraselmis sp., isolated from Ganghwa island in a previous study, a rapid-growing strain with 237% higher biomass productivity and an oleaginous strain with twice higher fatty acid contents at 10°C were isolated. The oleaginous Tetraselmis strain showed the highest fatty acid productivity among the strains, having 438% higher productivity than the previous strain. Using the new isolates in the seasons with low seawater temperature would improve microalgal fatty acid productivity in ocean cultivation.
신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구
박한울 ( Hanwool Park ),임경준 ( Kyung June Yim ),민지호 ( Ji-ho Min ),강성모 ( Sung-mo Kang ),한찬우 ( Chan-woo Han ),이창수 ( Chang-soo Lee ),정지영 ( Ji Young Jung ),홍성주 ( Seong-joo Hong ),이철균 ( Choul-gyun Lee ),김지훈 ( 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
본 연구에서는 국내 낙동강 수계에서 신규하게 분리된 미세조류인 Parachlorella sp. 종의 바이오매스 및 지방산 생산성에 대한 배지의 영향을 연구하였다. 미세조류 배양에 통상적으로 사용되는 BG-11, TAP, BBM 배지를 사용하여 바이오매스 생산성은 TAP 배지에서, 지방산 축적은 BBM 배지에서 가장 잘 일어나는 것으로 확인되었고, 지방산 생산성을 향상시키기 위해 암모니아와 아세트산을 사용하는 TAP 배지의 조성을 변화하여 BBM 배지처럼 지방산 축적을 유도하며 바이오매스 생산성을 증가시킨 MTAP 배지를 개발하였다. 전체적인 바이오매스와 지방산 생산성을 높이기 위해서는 MTAP-1 배지가 적합하여 바이오매스 생산성과 지방산 생산성은 기존의 TAP 배지 대비 각각 14%, 45% 증가하였다. 생리 활성 효과로 인해 관심도가 높은 오메가-3 지방산의 생산에는 MTAP-4 배지가 가장 적합하여 바이오매스 생산성과 오메가-3 지방산 생산성이 기존 BBM 배지 대비 각각 18%, 39% 증가하여 목표 중점 생산물질(바이오매스, 총 지방산, 또는 오메가-3 지방산)의 생산성을 향상시킬 수 있는 신규 배지 2종의 조성을 개발하였다. Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.
Atrioventricular Flow Wave Patterns before and after Birth by Fetal Echocardiography
김한울,이효연,백수진,홍영미 한국심초음파학회 2012 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.20 No.2
Background: Doppler echocardiographic measurements of both valves during intrauterine life can be used to calculate peak early filling velocity (E)/late peak atrial filling velocity (A) ratio as a single index of diastolic performance. The purposes of this study were to estimate the changes in atrioventricular valve flow from gestational age 37-40 weeks to 1 month of postnatal life and to clarify the difference in right and left ventricular diastolic filling patterns. Methods: Atrioventricular flow waves were analyzed in 24 full-term pregnant women by fetal echocardiography. Postnatal follow-up studies were performed at 1 hour, 6 hours, 24 hours, 3 days, 1 week and 1 month. In each time point, pulsed Doppler echocardiography was used to interrogate Doppler waveform of E velocity, A velocity, total area under the curve (time velocity integral) and heart rate. Results: Mitral E/A ratio significantly increased from 0.7 ± 0.1 before birth to 1.0 ± 0.3 at postnatal 1 hour, 1.0 ± 0.2 at 1 week,and 1.5 ± 1.0 at 1 month. Tricuspid flow E/A ratio was 0.8 ± 0.3 before birth, 0.8 ± 0.1 at 1 hour, 0.8 ± 0.2 at 3 days, 0.9 ± 1.0 at 1month. Time velocity integral of tricuspid flow was significantly higher than that of mitral flow before birth, but there was no difference after birth. Conclusion: The dominance of mitral A wave before birth was changed very quickly after birth to the dominance of E wave,but the dominance of tricuspid A wave was maintained at 1 month. Diastolic function and compliance of mitral valve were better than those of the tricuspid valve after birth.
Chlamydomonas reinhardtii dZL 균주의 광도가 세포 생장과 카로티노이드 생산량에 미치는 영향 연구
홍성주,이철균,김현우,민지호,박한울,김지훈,이창수,진언선 (사)한국해양바이오학회 2023 한국해양바이오학회지 Vol.15 No.2
Microalgae, as photosynthetic organisms, possess the ability to produce a diverse array of bioactive compounds. This study focused on the transformant Chlamydomonas reinhardtii dZL and subjected it to cultivation under varying light intensities (60, 120, 180, and 240 μmol/m2/s). Our aim was to assess the impact of light intensity on both microalgal biomass and carotenoid production. The cultivation took place in 80 mL bubble column photobioreactors, specifically the Multi-cultivator. Notably, the culture exposed to 240 μmol/m2/s exhibited the most rapid cell growth, surpassing even the cell concentration achieved at 180 μmol/m2/s by day 8. A detailed analysis of the specific irradiance rate over time unequivocally revealed a sharp decline in growth rates when the rate fell below 2 × 10-10 μmol/cell/s. Although the culture with 60 μmol/m2/s yielded the highest carotenoid content (1.2% of dry weight), the culture exposed to 240 μmol/m2/s recorded the highest carotenoid concentration at 8.9 mg/L owing to its higher biomass. Our findings reveal the critical importance of maintaining a specific irradiance rate above 2 × 10-10 μmol/cell/s to enhance biomass and carotenoid productivity. This study lays the groundwork for defining optimal light intensity conditions applicable to mass culture systems, with the objective of augmenting C. reinhardtii biomass and optimizing carotenoid productivity.
Beneficial effect of bacteria on biomass productivity of microalgae
유단비,홍성주,박한울,이철균 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Biomass productivity is one of crucial factors in microalgal biofuels production. Agal growth-promoting bacteria were isolated and their effects on algal biomass productivity was investigated. From a culture of a green microalga, Tetraselmis sp. MBEgh01L, three strains of bacteria were isolated. Each isolates was added to cultures of Tetraselmis sp. MBEh04Gc, which were pre-treated with antibiotics, to investigate the effects of the isolates on microalgal biomass productivity. Antibiotics-treated algal culture without addition of bacteria was used as control. The additions of bacteria increased algal biomass productivity in all cultures compared with the control. Each bacterial strains showed different degree of biomass productivity enhancements from 24% to 76%. The mechanism of algal growth promoting effect will be investigated as the further study, and the knowledge could provide another option to increase algal biomass productivity by creating a beneficial phycosphere.