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AFLP fingerprinting법을 이용한 담죽엽의 감별법 연구
심영훈,성락선,박주영,조창희,김지연,이종화,현성예,김선호,김동섭,장승엽 한국생약학회 2010 생약학회지 Vol.41 No.4
Lophatheri Herba is the aerial part of Lophatherum gracile Bronghiart(淡竹葉, Gramineae). 25~75 cm in length. Stem: cylindrical with nodes, empty inside, externally pale yellowish green. Leaf: dehiscent of lanceolate lamina, shrunken and rolled, 5~20 cm long, 10~35 mm wide; surface: pale green ~ yellowish green, parallel-formed with veins of square reticulate,more distinct of appearance on the lower surface. Banbusae Caulis In Taeniam is the stringy strip derived from the stem with the peeled-off epidermis of Phllostachys nigra Munro var. henosis Stapf, and Phllostachys bambusoides Siebold et Zuccarini (竹葉, Gramineae). Irregular in size and shape, thin plane ~ strip-shaped, sometimes powdery, sometimes 1~3 mm thick. Outer surface: pale green ~ yellowish green, sometimes grayish white L. gracile and P. nigra have different origins although they show similar morphologic features. We were able to distinguish between L. gracile and P. nigra which are almost indistinguishable through this study. AFLP(Amplifide Fragment Length Polymorphism) was more suitable for identifying differences between L. gracile and P. nigra in comparison with other genetic analysis using chemical analysis. Therefore. molecular biological methods are believed to be useful for discovering origins of herbal medicines.
Elevated Plus-maze를 이용한 Phenylpropanoid의 항불안 효과
윤병훈(Byung Hoon Yoon),최지웅(Ji Woong Choi),정지욱(Ji Wook Jung),신진선(Jin Sun Shin),현성예(Seong Ye Hyeon),정재훈(Jae Hoon Cheong),류종훈(Jong Hoon Ryu),고광호(Kwang Ho Ko) 대한약학회 2005 약학회지 Vol.49 No.5
The purpose of this study was to characterize the putative anxiolytic-like effects of phenylpropanoids using the elevated plus maze (EPM) test in mice. Cinnamic acid, p-coumaric acid, caffeic acid and ferulic acid were orally administered to male ICR mice, 1 h before behavioral evaluation in an EPM, respectively. Control mice were treated with an equal volume of vehicle, and positive control mice diazepam(1 mg/kg). A single treatment with phenylpropanoids (at 8 mg/kg) significantly increased time-spent and arm entries into the open arms of the EPM., and decreased time-spent and arm entries into the closed arms of the EPM versus control (P〈 0.05). However, no changes in the locomotor activity and myorelaxant effect were seen in any group versus the saline control. These results suggest that phenylpropanoids may be an effective anxiolytic agent.