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허준,변성진 경희대학교 부설 조경계획연구소 1996 造景論叢 Vol.8 No.1
At the time of research, middle school students gave rise to lots of social problems. It might be remedied by the proper education and leisure in a way. It was the physical activity that they liked most to any other. But the lack of physical education and facility could not be adequate to their needs. So this research was carried out to employ the urban park in the physical education and leisure space. And the followings were the things which were connected with the solution. It was not what all kinds of park should be physical park but the park which had completed facilities or not could be used as it, because there was a physical park in the current urban park act. A self-governing body had to realize the lack of physical education space and facility so as to establish an ordinance. And the problems which could be occurred during school hours could be solved by grouping schools which lay adjacent to a park. So walking and shuttle bus could help it.
Negative Cooperative Binding of Ethidium with Calf Thymus DNA in the Presence of Spermine
허준,고동성 생화학분자생물학회 1988 BMB Reports Vol.15 No.4
Based on Scatchard plots of equilibrium binding of spermine to the DNA, the number of total binding sites of spermine per nucleotide of the DNA was estimated to be n=0.016. In the presence of spermine at the concentration to cover the total binding site on n=0.016 per nucleotide completely under the same conditions, an empirical Hill plot of the data of spectrophotometric titration of ethidium bromide with DNA shows negative cooperativity of the binding, having the Hill constant of n=0.39, while in the absence of spermine, the plot shows noncooperative binding of ethidium. From the binding characteristics of spermine with the DNA arid the differential effect of spermine on the binding characteristics of ethidium with the DNA, it can be speculated that spermine bound to a section of the DNA would exert influence alter the cooperative conformational characteristics of the spermine-free section of DNA through allosteric propagation of the conformational transition induced by the binding of the spermine. Possible applicability and usefulness of this concept for the elucidation of molecular biological problems of other and/or higher structural-level biosystems is discussed. *This was supported by the Korea Science and Engineering Foundation Grant.
Leucine-C¹⁴에 依한 Puromycin이 白鼠顎下腺의 蛋白合成에 미치는 影響에 關한 自記放射法的硏究
許濬 최신의학사 1971 最新醫學 Vol.14 No.11
Male Balb C/Strain were used in all experiment. They ranged in weight from 18 to 23 gm. Puromycin dihydrochloride was injected intraperitoneally in aqueous solution, in a concentration of 2mg. /ml. , which had been neutralized with naoh. Two doses of 80mg/kg body weight each were given on day 1 intervals. Following the last injection, 3 pairs each were sacrificed on days 4, 6, 10 and 15. Thirty minutes prior to sacrifice 49c/gm body weight of leucine-C" (Specific Activity 278mc/mM) were injected. For the light microscopic studies, tissue were fixed 2% paraformaldehyde and sections were stained with toluidine blue o. The purpose of the present study was to determine, by means of quantitative autoradiography, the effect of puromycin on the protein synthesis of submandibular gland cells. 1. Following administration of puromycin to mice for 10 days, many of the acinar cells are lack the normal basophilia, increase of the vacuoles and the nuclear pyknosis. 2. We have observed that the protein synthesis as expressed by the incorporation of leucine-C" into submandibular gland cells is suppressed during first 4 days after the puromycin injections, but was significantly suppressed by 10 days. The grain number in puromycin-treated animals increased to a level similar to that of the controls by 15 days. 3. It is concluded that the synthesis of overall cytoplasmic proteins is temporarily impaired after puro-?mycin injections in submandibular gland cells of mice.
허준 해군대학 1997 海洋戰略 Vol.- No.94
이 글은 미 해군대학(Naval war college)의 석사학위를 가지고 있고, 동대학 전략연구소의 연구원으로 재직중인 미 해군중령 Murdock이 1996년 2월호 Proceeding 지에 기고한 “SSNs Aren't Enough"를 번역한 것이다. Murdock 중령은 걸프전쟁시 미함 Jarret(FFG-33)함의 부장으로 근무하기도 했다. 1. 문제의 제기 2. 임무 3. 적용교리와 능력 4. 연안전투 잠수함
Genome sequence analysis of Sphingomonas histidinilytica C8-2 degrading a fungicide difenoconazole
허준,박인철,한병학,권순우,이세원,안재형 한국미생물학회 2019 미생물학회지 Vol.55 No.4
A bacterial strain C8-2, which can rapidly degrade a triazole fungicide difenoconazole, was isolated from an agricultural soil. The genome of strain C8-2 was sequenced and compared with its non- difenoconazole-degrading relative, Sphingomoans histidinilytica UM2 T . The OrthoANI value between the two strains was 99.3% thus strain C8-2 was identified as S. histidinilytica. The genome of strain C8-2 is composed of one chromosome and one plasmid, with a total size of 5,758,685 bp, a G + C content of 67.2%, 5,393 coding genes (CDS), 114 pseudogenes and 59 RNA genes. Strain C8-2 harbors seven aromatic ringhydroxylating dioxygenases not found in strain UM2 T , and two of them showed high amino acid sequence identities (more than 96%) with the angular dioxygenases previously reported to hydroxylate the diphenyl ether group of 3-phenoxybenzoate, yielding 3-hydroxybenzoate and catechol.