의류제품의 치수 규격동향

한상미,Han, Sang-Mi 기술표준원 2003 기술표준 Vol.15 No.-

채취 시기 및 지역에 따른 봉독의 성분 분석

한상미,윤형주,백하주,Han, Sang-Mi,Yoon, Hyung-Joo,Baek, Ha-Ju 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.3

서양종 꿀벌 독의 채집시기와 지역에 따른 봉독의 성분 변화 및 약리효과에 미치는 영향을 검토하였다. 채집시기는 5월부터 9월까지, 채집지역은 전국 35개 지역으로부터 채취한 봉독을 대상으로 2010년과 2011년 2년에 걸쳐 동일 지역에서 동일한 방법으로 봉독을 채취하였다. 채취한 봉독은 액체크로마토그래피를 통해 멜리틴과 아파민 그리고 포스포리파아제 A2의 성분 함량을 분석하였다. 그 결과 채집시기와 지역에 따른 성분에 유의한 차이는 확인되지 않았다(One way-ANOVA, Duncan's test (${\alpha}$=0.05)). 봉독의 성분은 채집시기와 지역에 관계없이 멜리틴 $55.2{\pm}2.07%$, 아파민 $2.57{\pm}0.103%$ 그리고 포스포리파아제 A2는 $12.51{\pm}0.37%$을 차지하였다. 이상의 결과로부터 봉독은 채취시기에 따른 주요 성분은 차이를 갖고 있지 않았으며, 이는 꿀벌의 먹이, 사육온도 등 외부 환경이 봉독 분비에 영향을 주지 않는 것으로 사료되었다. This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).

호박벌 일벌독의 성분 분석 및 생리활성 탐색

한상미,이광길,여주홍,권해용,우순옥,윤형주,김미애,김원태,백하주,Han, Sang-Mi,Lee, Kwang-Gill,Yeo, Joo-Hong,Kweon, Hae-Yong,Woo, Soon-Ok,Yoon, Hyung-Joo,Kim, Me-Ae,Kim, Won-Tae,Baek, Ha-Ju 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2

호박벌 일벌독의 성분과 생리활성을 규명하기 위하여 단백질 성분분석과 암세포 생육 저해 효능, 항균력을 검토하였다. 이차원단백질 분석을 통해 호박별의 일벌독은 63개의 단백질이 존재하는 것으로 확인되었으며, 가장 많은 함량을 보이는 3개의 단백질을 염기서열 분석하였다. 그러나 이들 성분은 아직 밝혀지지 않은 성분으로 판단되었다. 호박벌 일벌독의 암세포 (간암; Hep3B, 폐암; A549, 유방암; BT-20, 위암; AGS) 에 대한 생육 저해능은 시료 농도가 증가함에 따라 증가하는 경향을 보이며 100ng/ml에서 간암세포 (Hep3B) 에 대한 생육 저해능이 55%로 가장 높았다. 항균활성에 E. faecium 과 S. sonnei에 대하여 최소발육억제농도와 최소살균농도 모두 각각 0.256ug/ml 로 강한 항균활성을 나타내었으며, 그 외의 피검균에 대해서도 비교적 높은 활성을 보였다. 이상의 결과로부터 호박벌 일벌독의 성분은 다른 벌의 독성분과는 차이를 보이며, 그 생리활성에 있어 약리학적 이용이 가능할 것으로 판단되었다. Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1ng/ml to 100ng/ml. Expecially, the treatment of 100ng/ml B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256ug/ml, respectively.

무당벌레(Harmonia axyridis) 추출물에 의한 BV-2 세포주의 Nitric Oxide 생성 저해 활성

한상미,이상한,윤치영,강석우,이광길,김익수,윤은영,이평재,김선여,황재삼,Han Sang-Mi,Lee Sang-Han,Yun Chi-Young,Kang Seok-Woo,Lee Kyung-Gill,Kim Ik-Soo,Yun Eun-Young,Lee Pyeong-Jae,Kim Sun-Yeou,Hwang Jae-Sam 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

퇴행성 뇌질환은 뇌에 존재하는 면역세포인 소교세포의 염증반응이 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 초고속자동화시스템(high throughput screening: HTS)을 이용하여 약용곤충추출물로부터 항산화와 항염증 기능이 있다고 알려진 무당벌레 추출물로부터 염증발생인자인 nitric oxide의 생성에 어떠한 영향을 주는지 다음과 같은 결과를 얻었다. 소교세포인 BV-2세포에 대한 무당벌레 추출물의 세포독성은 물과 메탄올, DMSO 추출물에서는 100 ng/ml 까지는 거의 없었으나 에탄올 추출물은 1 ng/ml에서도 세포독성이 있었다. 물과 메탄을 추출물(50 ng/ml)은 LPS로 활성화된 BV-2세포에서 $TNF-{\alpha}$와 $IL-1{\beta}$의 발생을 35-60% 가량 억제 하였다. LPS로 유도된 NO의 생성은 물과 메탄올 추출물을 처리했을 때 각각 55%, 76% 억제되었다. 또한, MeOH 추출물을 처리했을 경우 LPS에 의한 iNOS 발현 정도를 단백질 수준과 mRNA 수준에서 현저하게 억제시킴을 확인하였다. Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water$(H_2O)$ and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of $H_2O$ of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.

국산 프로폴리스의 Helicobacter pyrori 억제효과

한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

서양종 꿀벌로부터 분리한 정제봉독의 통풍 억제 효능 구명

한상미(Sang Mi Han),우순옥(Soon Ok Woo),김세건(Se Gun Kim),김효영(Hyo-Young Kim),최홍민(Hong Min Choi),문효정(Hyo Jung Moon) 한국양봉학회 2019 韓國養蜂學會誌 Vol.34 No.1

Honeybee (Apis mellifera L.) venom (PBV) is a good candidate for development as a therapeutic modality. The gout is a form of inflammatory arthritis caused by excess uric acid in the bloodstream. Gout causes sudden severe joint pain. To investigate in vitro xanthine oxidase inhibitory properties of PBV for gout. PBV was studied for in vitro xanthine oxidase inhibitory activity using a spectrophotometric method and matrix metalloproteinase (MMP)-9 expression after 24 hr of monosodium urate crystal (MSU) treatment when MMP-9 production reached a maximum in RAW 264.7 cells. The highest xanthine oxidase inhibitory activity was 94.7% in the 10 μg/mL PBV. The treatment of 10 μg/mL PBV showed an inhibitory effect of MMP-9, gout inflammatory marker. PBV was evaluated the xanthine oxidase and MMP-9 inhibitory potential in our study. We suggest that PBV could be used an excellent substance for gout prevention.

생물학적 원료의약품 사용을 위한 꿀벌 봉독의 바이러스 안전성 검증

한상미(Sang Mi Han),우순옥(Soon Ok Woo),김세건(Se Gun Kim),장혜리(Hye Ri Jang),최홍민(Hong Min Choi),문효정(Hyo Jung Moon) 한국양봉학회 2018 韓國養蜂學會誌 Vol.33 No.3

Honeybee (Apis mellifera L.) venom (BV) is a good candidate for development as a therapeutic modality for the treatment of acne vulgaris. However, BV has been restricted in the development of medical agents because BV has not been registered as an Active Pharmaceutical Ingredient (API). The aim of this study was to evaluate viral safety of BV, as biological origin agents. The viral diseases of honeybees were reported at least 18 virus types. Using RT-PCR we evaluated infected honeybees, BV isolated bee venom sac from virus infected honeybee, and BV collected from honeybees using BV collector for the presence of the seven bee viruses, complete genome sequence including Sacbrood virus (SBV), Black queen cell virus (BQCV), Deformed wing virus (DWV), Chronic bee paralysis virus (CBPV), Acute bee paralysis virus (ABPV), Kashmir bee virus (KBV), and Israeli acute paralysis virus (IAPV). The SBV, BQCV, DWV, CBPV, ABPV, KBV, and IAPV were not detected both BV isolated bee venom sac from virus infected honeybee and BV collected from honeybees using BV collector. According to these results, we have meanwhile observed that BV and BV sac might be safety for bee viruses contamination. We suggest that BV could be used a good candidate for API.

Bacillus cereus, Streptococcus agalactiae와 Pseudomonas aeruginosa에 대한 정제봉독의 항균효과

한상미(Sang Mi Han),우순옥(Soon Ok Woo),김세건(Se Gun Kim),장혜리(Hye Ri Jang),이경우(Kyung Woo Lee) 한국양봉학회 2018 韓國養蜂學會誌 Vol.33 No.1

Bacillus cereus, Streptococcus agalactiae and Pseudomonas aeruginosa can cause disease in plants and animals, including humans. A species of considerable medical importance, they are a multidrug resistant pathogen recognised for their ubiquity, its intrinsically advanced antibiotic resistance mechanisms, and its association with serious illnesses - hospital-acquired infections such as ventilator-associated pneumonia and various sepsis syndromes. B. cereus, S. agalactiae and P. aeruginosa were tested for antibacterial effects to purified bee venom (Apis mellifera L.). We evaluated the antibacterial effect of purified bee venom by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill assay and postantibiotic effect (PAE). Purified bee venom exhibited significant inhibition of bacterial growth of B. cereus, S. agalactiae and P. aeruginosa with MIC value of 1.835±0.202, 0.54±0.32 and 0.056±0.01μg/ml, respectively. The MBC value of purified bee venom against B. cereus, S. agalactiae and P. aeruginosa were 33.9± 1.23, 4.08±0.3 and 0.59±0279μg/ml. In the presence of 2×MIC concentrations of the purified bee venom, indicating that growth of all bacteria was also significantly inhibited within 4 hours. Furthermore, the results of PAE values against B. cereus, S. agalactiae and P. aeruginosa showed the bacterial effect with 1.5, 1.5 and 5.5 hr. In this suty, these results were suggested that purified bee venom might be utilized as a natural antibiotic agents in animal diets.

정제봉독의 반복 첩포 피부자극시험

한상미(Sang Mi Han),홍인표(In Phyo Hong),우순옥(Soon Ok Woo),김세건(Se Gun Kim),장혜리(Hye Ri Jang),박관규(Kyun Kyu Park) 대한약학회 2017 약학회지 Vol.61 No.1

This study was performed to examine the repeated skin irritation reaction of purified bee venom (Apis mellifera L.) collected using bee venom collector. To confirm whether the purified bee venom causes primary skin irritation when used for the skin medicinal products, skin irritation testing was conducted using rabbit models. The purified bee venom (0.5 mg/kg/day) was uniformly applied on each gauze patch cut into a size of approximately 2.5 cm×2.5 cm. Then, each patch was pasted to the skin, respectively, and was fixed with an adhesive plaster. The left was controls and the right was purified bee venom for 7 days everyday. Observations were made at 1, 24, 48 and 72 hours after the patch had been removed, and then the irritation reaction was scored according to Formation of erythema and eschar and edema. No irritation reaction was observed in any of the test skins during the observation period. Therefore, the P.I.I, was 0. As a result, no irritation reaction was observed in any of the test skins during the observation period. Therefore, in the primary skin irritation test using rabbits, the purified bee venom was judged to belong to a category “non-irritant”. This study demonstrated that purified bee venom has promising potential external treatment for topical uses that do not induce significant levels of skin reaction.

정제봉독의 ADH와 ALDH 활성 효과

한상미(Sang Mi Han),홍인표(In Pyo Hong ),우순옥(Soon Ok Woo),김세건(Se Gun Kim),장혜리(Hye Ri Jang) 한국양봉학회 2017 韓國養蜂學會誌 Vol.32 No.3

We investigated whether purified bee venom increases the enzymatic activity of the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). ADH and ALDH assay were tested by in vitro kits. The purified bee venom was assayed by ultra performance liquid chromatography, The contents of melittin, apamin and phospholipase A2, as main component of purified bee venom, were 63.9%, 2.3%, and 10.9%, respectively. The ADH and ALDH acitivity of purified bee venom(at 1mg/ml) were 88.6±7.34% and 94.6±0.57%, respectively compared with positive control at 2mg/ml. These results showed that purified bee venom induces the activity of ADH and ALDH which reduce the aldehyde concentration in the blood, suggesting the possibility of purified bee venom as resource of medicine or functional beverage for hangover relieving.