어성초로부터 분리된 Quercetin의 카드뮴에 대한 독성억제효과

최화정,노용주,강정일,백승화,Choi, Hwa-Jung,No, Yong-Ju,Kang, Jeong-Il,Baek, Seung-Hwa 대한약학회 2009 약학회지 Vol.53 No.1

The aim of this study was to develop antitoxic compound for cadmium-induced cytotoxic Vero cells. These cells were divided into five groups; control group (medium only), cadmium group (cadmium only), and two experimental groups. SRB (sulphorodamine B) assay was performed to evaluate the cytotoxicity of cell organelles. After cadmium was treated on Vero cells, we determined IC50 values to examine the detoxification effects of Houttuynia cordata methanol extract and quercetin under cadmium-induced cytotoxicity. Furthermore, morphological changes were observed by the light microscope. In Vero cells, methanol extract of Houttuynia cordata, and quercetin showed inhibitory effects on cadmium-induced cytotoxicity and these detoxification effects were increased in a concentration-dependent manner. These results suggest that methanol extract and quercetin from Houttuynia cordata retain a potential antitoxic activity.

Streptococcus thermophilus로 발효한 한약재 발효분말의 항염증 및 미백 효과

최화정 ( Hwa-jung Choi ),이정희 ( Jung-hee Lee ),윤미영 ( Mi-young Yun ),이재숙 ( Jae-sug Lee ) 대한화장품학회 2015 대한화장품학회지 Vol.41 No.2

새로운 항염증 및 미백 소재를 찾기 위해서, 본 연구에서는 6가지 한약재 추출물(유자, 상백피, 오미자, 율무, 당귀, 고삼)을 Streptococcus thermophilus에 의해 발효한 발효분말의 미백 및 항염증 효과를 피부섬유 아세포에 대한 피부독성, 산화질소 생성, tyrosinase 활성, 멜라닌 형성의 저해 효과를 측정함으로써 평가하였다. 발효물은 37 ℃에서 2일 동안 Streptococcus thermophilus에 의해 발효한 후, 동결건조에 의해 발효분말을 제조하였다. 발효분말은 피부섬유아세포에 대해 500 μg/mL의 농도에서 cytopathic effect reduction 방법을 사용하면서 측정하였을 때 세포독성을 나타내지 않았다. 또한 발효분말은 Griess reagent system을 사용하면서 산화질소 생성에 대한 저해 효과를 나타내었다. 더욱이 발효분말은 tyrosinase 활성에 대한 저해 효과가 농도 의존적으로 나타났다. 발효분말은 배양액으로부터 멜라닌 생성에 대해 유의적인 저해를 나타내었다(p < 0.05). 그러므로 이러한 자료로부터 발효분말은 항염증 및 미백 효과를 갖는 것으로 나타났으며, 화장품을 위한 효과적인 성분으로써 사용가능할 것으로 사료된다. To identify new anti-inflammatory and whitening material, this study investigated the whitening and anti-inflammatory effects of the lyophilized powder from 6 oriental plant extracts (OPE; Citrus junos Tanaka, Mori cortex Radicis, Schisandra chinensis Baillon, Coix lachrymajobi var. mayuen, Angelica gigas NAKAI, and Sophora japonica L.) fermented with Streptococcus thermophilus by assessment of cytotoxicity on human dermal fibroblast, inhibitory effect of nitric oxide (NO) prodction, tyrosinase activity and melanin formation. The OPE was fermented with Streptococcus thermophilus at 37 ℃ for 2 days and the lyophilized powder was manufactured by freezing-dryer. OPE didn’t show cytotoxicity at concentration of 500 μg/mL using a cytopathic effect reduction method. OPE also exhibited inhibitory effect on nitric oxide (NO) prodction by Griess reagent system. Furthermore, OPE showed inhibitory effect on tyrosinase activity with dose dependent manner, and exhibited significant inhibition of melanin formation by measurement of melanin from culture media (p < 0.05). From these results, 6 OPE extracts showed anti-inflammatory and whitening effect and may be used as an active ingredient for cosmetics.

어성초로부터 분리된 Quercetin의 Protein Tyrosine Phosphatase 1B 활성

최화정(Hwa Jung Choi),배은영(Eun Young Bae),노용주(Yong Ju No),백승화(Seung Hwa Baek) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.6

손바닥 선인장(Opuntia humifusa) 발효액의 화학적 성분과 자궁경부암 세포주에 대한 항암작용

최화정(Hwa-Jung Choi),박승춘(Seung-Chun Park),홍태희(Tae-Hee Hong) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.10

손바닥선인장(Opuntia humifusa)의 줄기, 뿌리 그리고 잎 발효액의 화학성분 비교 및 자궁경부암 세포주에 대한 항암 활성을 조사하였다. 줄기 발효액의 열량, 조단백, 조지방 그리고 조탄수화물의 성분비율은 86.21 kcal, 0.92%, 0.12% 그리고 20.34%로 분석되었다. 열매에서는 65.32 kcal, 0.08%, 1.04% 그리고 15.15%로 각각 구성되었다. 무기물 분석에서 줄기와 열매의 발효액에 칼슘(calcium)과 철(ferrous)의 농도는 100 g 당 1,800 ㎎과 388 ㎎ 그리고 21 ㎎과 10 ㎎으로 분석되었다. 발효 전에 물, 메탄올 그리고 에탄올 추출물에 대하여 항암활성을 측정한 결과 자궁경부암 세포주인 CaSki, SiHa 그리고 HaCaT에서 어떠한 항암활성도 보여주지 않았다. 그러나 손바닥선인장(Opuntia humifusa)의 열매를 효모균으로 발효한 발효액에서 HaCaT를 제외한 CaSki와 SiHa에 대해서는 항암활성을 보여주었다. The purpose of this study is to investigate anti-tumor activities, general composition, elemental composition and mineral contents of fermented liquid stem, root and fruit of Opuntia humifusa. In the general composition, the energy, crude protein, crude lipid and crude carbohydrate contents of fermented liquid stem were 86.21 Kcal, 0.92%, 0.12%, and 20.34%, respectively. Fermented liquid fruit showed 65.32 Kcal, 1.04%, 0.08%, and 15.15%. In mineral analysis, fermented liquid stem and fruit showed 1,800 and 388 mg of calcium per 100 g. The ferrous concentrations of fermented liquid stem and fruit were 21 and 10 mg per 100 g, respectively. Methanol, ethanol and water extracts of nonfermented liquid stem and fruit did not inhibit the proliferation in human cervical cancer cells (CaSki, SiHa and HaCaT), but the fermented liquid fruit showed the inhibition of proliferation with dose-response manner in CaSki and SiHa cells, but not HaCaT. Therefore, it suggests that fermented cactus may be used as one of potential adjuvant for the treatment of cervical carcinomas.

Streptococcus thermophilus로 발효된 백년초 발효물의 피부생리활성 평가

최화정 ( Hwa Jung Choi ),정주임 ( Jung Ju Im ) 한국미용학회 2017 한국미용학회지 Vol.23 No.3

To identify materials possessing adermal bioactive properties, this study was conducted the effects of Opuntia ficusindica fruits fermented with Steptococcus thermophilus (OFST) on cytotoxicity of human dermal fibroblast, antioxidant activity, collagen biosynthesis and matrix metalloproteinase-I (MMP-1) activity. As the results, the number of viable bacteria for OFST after fermentation for 12 h showed 0.4 log CFU/mL and it exhibited 8 log CFU/mL after fermentation for 24 h. The initial pH of OFST was 7.2. The pH between 12-24 h was 6.4 and 4.7, respectively, The pH of OFST after fermentation for 24 h was 4.5. The OFST didn`t show cytotoxicity against human dermal fibroblast at tested concentration. The OFST didn`t show cytotoxicity against human dermal fibroblast at concentration of 1-100 μg/mL. In antioxidant activity, OFST showed significant result of scavenging action over 10 μg/ml. The OFST have significantly antitoxidant activity with dose-dependent manner. In biosynthesis of collagen, OFST represented a meaning effect. It was increased with dosedependent manner over 10 μg/ml. In effect on collagen synthesis, TNF-α treatment was appeared to have a effect on increasing MMP-1 activity compared with TNF-α nontreatment. However, OFST treatment on increased MMP-1 activity was decreased it. The significant effect of OFST on MMP-1 activity was showed at 50 μg/ml and 100 μg/ml. According to this results of the study, OFST will be able to suggest as effective dermal bioactive materials.

Antiviral Activity of Zanthoxylum Species against Influenza Virus

최화정(Hwa Jung Choi),송재형(Jae Hyoung Song),권두한(Dur Han Kwon),백승화(Seung Hwa Baek),안용준(Young Joon Ahn) 한국약용작물학회 2008 한국약용작물학회지 Vol.16 No.4

Eupafolin과 eupatilin의 미백 및 주름개선 효과

최화정 ( Hwa-jung Choi ),정주임 ( Ju-im Jung ) 한국미용학회 2019 한국미용학회지 Vol.25 No.2

Consumers are increasingly looking to natural ingredients to improve the appearance of their skin and delay the effects of aging. In our article, cytotoxicity of eupafolin and eupatilin isolated from Artemisia princeps was evaluated in in human dermal fibroblast cell and B16F10 melanoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Also, the effect of eupafolin and eupatilin on tyrosinase activity and α-melanocyte stimulating hormone (α-MSH) induced melanin synthesis in B16F10 melanoma cells were evaluated. Furthermore, the effect of eupafolin and eupatilin on collagen synthesis and tumor necrosis factor-α (TNF-α) induced Matrix Metalloproteinase-1 (MMP-1) expression investigated in human dermal fibroblasts. Eupafolin and eupatilin didn’t show cytotoxicity in human dermal fibroblast cells and B16F10 melanoma cells. The eupafolin and eupatilin significantly induced collagen synthesis from the skin fibroblasts (p<0.05) and also dramatically inhibited MMP-1 activity, which is the enzyme involved in collagen breakdown. Eupafolin and eupatilin significantly decreased melanin synthesis with inhibition of tyrosinase activity (p<0.05). Therefore, the eupafolin and eupatilin displayed a preventive effect when used for anti-aging purposes in human skin and may be an appropriate choice for cosmetic products that aim to provide dermal bioactive effects, and which are designated as anti-aging skin care products.

사자발쑥 추출물부터 분리된 Eupafolin과 Eupatilin의 DPPH 저해활성

최화정 ( Hwa-jung Choi ),정주임 ( Ju-im Jung ) 한국미용학회 2018 한국미용학회지 Vol.24 No.2

Natural products made from plant sources have been used in a variety of cosmetic applications as a whitening, wrinkle and inflammatory agent. The Artemisia princeps have been used as a traditional medicine for skin diseases and have been reported to possess anti-oxidant properties. Artemisia princeps was extracted with 100%, 80%, 50% methanol or ethanol and then total flavonoid content (TFC) and total phenolic content (TPC) of their extracts were measured using the sodium borohyride/chloranil-based assay and Folin-Ciocalteu reagent reaction, respectively. DPPH radical scavenging activity of their extracts were measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Fifty % methanolic extracts among the various extracts showed the highest TFC and TPC, and then 100% methanolic extracts showed the highest DPPH radical scavenging activity. Two flavonoids (eupafolin and eupatilin) were isolated from 100% methanolic extract of A. princeps. The DPPH radical scavenging activity of eupafolin and eupatilin was showed IC50 value of 35.7 μg/mL and 32.6 μg/mL by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Therefore, the eupafolin and eupatilin displayed a preventive effect when used for anti-oxidant purposes in human skin and may be an appropriate choice for cosmetic products that aim to provide dermal anti-oxidant effects.

In vitro 웰니스 화합물 (Ochnaflavone)에 의한 암세포 성장 저해

이재숙,최화정,김명주,박장순,Lee, Jae-Sook,Choi, Hwa-Jung,Kim, Myung-Ju,Park, Jang-Soon The Society of Digital Policy and Management 2015 디지털융복합연구 Vol.13 No.5

많은 식물들은 부작용이 적고, 가격이 저렴하며, 다양한 웰니스 융복합 화합물들을 함유하고 있기 때문에 다양한 제재에 이용되고 있다. 이 연구에서 뉴질랜드 식물인 Quintinia acutifolia (Q. acutifolia)로부터 쥐 백혈병 세포(P388 murine lymphocytic leukemia cells)의 성장을 저해하는 활성을 MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay에 의해 평가하였다. P388 murine lymphocytic leukemia 세포의 성장을 저해하는 2,3,2'',3''-tetrahydroochanaflavone (1)과 2'',3''-dihydroochana-flavone (3)을 1D/2D-NMR와 다른 분광학적 분석법에 의해 분리하였고, 구조를 규명하였다. 이 두 화합물은 두 개의 플라보노이드 기본구조를 갖는 바이플라보노이드 (biflavonoid)로써 2,3,2'',3''-Tetrahydroochnaflavone (1)과 2'',3''-dihydroochana-flavone (3) 화합물은 P388 murine lymphocytic leukemia세포에 대해 50%의 성장저해를 나타내는 농도가 각각 $8.2{\mu}g/mL$와 $3.1{\mu}g/mL$로 나타났다. 특히 2'',3''-dihydroochana-flavone (3) 화합물은 2,3,2'',3''-tetrahydroochanaflavone (1)의 B 링(ring)에 쌍으로 결합되지 않은 플라본 구조 (unconjugated flavonone system)를 갖는 것으로 나타났다. 그럼으로 두 화합물은 향후 항암 치료제 개발에 이용될 수 있으며, 더 많은 연구가 요구된다. Medicinal plants containing wellness-fusion-complex compound are increasingly being pursued as suitable alternative sources of various biological properties. In this study, inhibitory effect of Quintinia acutifolia, which is a New Zealand plant, on P388 murine lymphocytic leukemia cells using MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay. Based on $^1H-NMR$, $^{13}C-NMR$ spectral data and other spectral analysis, 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) inhibited the leukemia cells were purified from the plants. 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) are biflavonoids possessing two basic flavonoids and actively inhibited growth of P388 murine lymphocytic leukemia cells with a 50% inhibitory concentration ($IC_{50}$) of $8.2{\mu}g/mL$ and $3.1{\mu}g/mL$, respectively. Specially, 2'',3''-dihydroochana-flavone (3) possessed unconjugated flavonone system, which isn't consist of a pair with B ring of 2,3,2'',3''-tetrahydroochanaflavone (1). Therefore, the two compounds could be considered as a candidate for development of anticancer drugs and need to much studies in the future.

고삼으로부터 분리된 Flavanones의 항균효과

양희태,최화정,백승화,Young, Hee-Tae,Choi, Hwa-Jung,Baek, Seung-Hwa 대한약학회 2008 약학회지 Vol.52 No.4

Two known lavandulylated flavanones, leachianone A (1) and sophoroaflavanone G (2), were isolated from the roots of S. flavascens Ait. The structures of these compounds were determined on the basis of IR, 1D and 2D NMR in addition to direct comparison with authentic compounds. However, leachianone A (1) and sophoroflavanone G (2) did not have growth inhibition activity against any microorganisms (MIC, >200 ${\mu}g/ml$).