한국형 워터파크의 개발방향

최식원 경기대학교 관광종합연구소 2011 여가관광연구 Vol.18 No.-

관광지에서의 음식서비스 경험이 관광자만족에 미치는 영향

최식원 ( Shick Won Choi ),박석희 ( Suk Hee Park ) 경기대학교 관광종합연구소 2012 여가관광연구 Vol.19 No.-

한국의 국가 이미지에 대한 조사 결과 조사 대상의 절반이 넘는 52.4%가 ‘한국 하면 독특한 문화가 떠오른다’고 대답했고, 문화 관련 항목 중에서는 음식(51.8%)이 가장 강한 이미지로 나타났다(성균관대학교 국가브랜드 경영연구소, 2003: 38, 53). 이는 음식의 문화관광자원으로서의 가치를 시사하고 있으며, 외식업의 한 부분으로서의 음식이 아니라 관광활동, 더 나아가서는 문 화생활 등 폭 넓은 관심이 필요하다는 것을 말해주고 있다. 이에 본 연구는 음식에 대한 관심 과 중요도가 커짐에 따라 관광활동에서의 음식에 대한 불만 또한 증가하고 있는 것을 확인하고, 관광활동에서 음식과 관광전반만족에 어떤 관련성이 있는지 조사하였다. 경주를 방문 관광 객을 대상으로 실증분석 결과, 음식서비스에 대한 만족은 관광만족과 방문객 충 성도에 긍정적 인 영향을 미치고 그 중 음식가치 요인이 가장 큰 영향력을 보이는 것을 확인하였다. According to "Survey on National Image" conducted by National Brand Management Institute at Sungkyungwan Univ, half of respondents mentioned "culture" as one of Korea`s representing images. Among them, Korean food and beverage were taken account of 51.8%. This shows the potential of food and beverage as tourism resource and activity. In this regard, the purpose of this paper is to analyze the relations between food service and overall tourist satisfaction. Data were collected by conducting the survey to 398 available visitors in Gyeongju where one of the best tourist destination in Korea. The results are as follows: Firstly, food and beverage was one of big determinants of overall satisfaction. Secondly, the satisfaction on food and beverage made positive effect on visitors royalty. Thirdly, longer stayers was more affected by food and beverage service. This implies that there needs continuing study on the relations between tourist satisfaction and food service, so we can reach a general model on the role of food service. And also there needs ongoing evaluation and concern on food service so we can apply the result through different management technique to the practice.

한국형 워터파크의 개발방향

최식원 ( Shick Won Choi ) 경기대학교 관광종합연구소 2011 여가관광연구 Vol.18 No.-

Comparison of nutritional components (isoflavone, protein, oil, and fatty acid) and antioxidant properties at the growth stage of different parts of soybean [Glycine max (L.) Merrill]

서우덕,강재은,최식원,이광식,이미자,박기도,이진환 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2

This is the first study to investigate antioxidant capacities of isoflavones prepared using microwave-assisted hydrolysis method from different parts (seeds, leaves, leafstalks, pods, stems and roots) of soybean at growth stages. In addition, the fluctuations in the isoflavone, protein, fatty acid, and oil contents in R6-R8 (R6: beginning; R7: beginning maturity; R8: full maturity) seeds were confirmed. The R7 seeds exhibited the most predominant contents of isoflavones (1218.1±7.3 μg/g) in the following order: daidzein (48%)>genistein (35%)>glycitein (17%). The second highest isoflavone content was found in the leaves (1052.1±10.4 μg/g), followed by R8 seeds>roots>R6 seeds>leafstalks> pods; the stems exhibited the lowest isoflavone content (57.2±1.7 μg/g). Interestingly, daidzein showed the highest individual isoflavone content with remarkable variations (57.2-766.8 μg/g), representing 46-100% of the total isoflavone content. R8 exhibited higher protein, fatty acid, and oil contents than R6 or R7. Moreover, the antioxidant capacities against two radicals in different parts of soybean plant showed considerable differences depending upon the isoflavone content. Our results suggested that soybean leaves and seeds might be useful materials for functional foods.

복령 균핵의 에탄올 추출물에서 분리한 화합물의 조골세포 분화 촉진 및 파골세포 생성 억제 효과

이소라,김석주,문보욱,최식원,유림,이형원 한국산림과학회 2024 한국산림과학회지 Vol.113 No.1

복령(Wolfiporia hoelen (Fr.) Y.C.Dai & V. Papp)은 아시아 및 유럽 등 많은 나라에서 약용 및 식용으로 사용하고있는 중요한 약재이다. 복령의 주요 성분들의 약리 활성이 많은 연구를 통해 밝혀지고 있으며, 골대사에도 영향을 주는 것으로 나타났다. 이에 본 연구는 복령 균핵 50% 에탄올 추출물이 골질환 예방 및 천연 치료제의 소재로 활용 가능성이 있는지 알아보고자 하였다. 복령 균핵의 에탄올 추출물을 계통분획법에 따라 n-헥산, 디클로로메탄, 에틸아세테이트순으로 분획하여 C2C12세포에 처리하였으며, 디클로로메탄 분획물에서 C2C12 세포의 ALP 분화 활성이 대조구보다 약 29%가증가함을 알 수 있었다. 활성을 보인 디클로로메탄 분획물로부터 총 4개의 화합물을 분리하였으며, 기기분석 결과와 문헌을 참고하여 3α-dehydrotrametenolic acid, ergosterol, pachymic acid와 dehydrotumulosic acid로 동정할 수 있었다. 골대사 효능이 있는 지표물질을 찾기 위하여 4개의 화합물을 C2C12 세포의 ALP 분화 활성과 BMMs의 RANKL 유도 억제 활성을농도별(1, 3, 10, 30, 100 μM)로 평가하였으며, 세포독성 검정도 동일한 농도에서 수행하였다. 그 결과 분리된 화합물 중dehydrotumulosic acid의 3 μM 농도에서 ALP 활성이 160%로 나타났으며, BMP-2 대조구보다 약 24% 유의미하게 증가하였다. 또한 BMMs의 RANKL 유도 억제 활성은 dehydrotumulosic acid를 10-100 μM 농도로 처리할 때 대조구보다 약15-86%로 유의미하게 감소하였으며, 10 μM 농도에서 나타난 RANKL억제 활성은 세포독성과 다른 패턴으로 나타나는 것을 확인하였다. 본 연구결과를 토대로 복령의 균핵 에탄올 추출물이 골모세포 분화를 증진시키거나 억제할 수 있는 가능성을 확인하였으며, 복령의 디클로로메탄 분획물로부터 분리 정제된 dehydrotumulosic acid가 골다공증 예방 및 치료에 도움을 줄 수 있을 것으로 사료된다. Wolfiporia hoelen (Fr.) Y.C.Dai & V. Papp, commonly known a s Poria cocos, is a significant traditional herb used for medicinal and culinary purposes Asian and European countries. Many studies have confirmed that the main components of W. hoelen have pharmacological activities and thatits extract has been shown to affect bone metabolism. This study aimed to the potential of a 50% ethanol extract of the sclerotium of W. hoelen for preventing and treating bone diseases. The ethanol extract was systematically fractionated using n-hexane, dichloromethane, and ethyl acetate. The dichloromethane fraction caused an approximately 29% increase in alkaline phosphatase (ALP) differentiation activity in C2C12 cells compared to the control. Four compounds isolated from this active dichloromethane fraction were identified through instrumental analysis and literature references as 3α-dehydrotrametenolic acid, ergosterol, pachymic acid, and dehydrotumulosic acid. All four compounds were evaluated at increasing concentrations (1, 3, 10, 30, and 100 μM) to determine their effects on ALP differentiation activity in C2C12 cells and RANKL-induced inhibition activity in bone marrow macrophages (BMMs), with a concurrent assessment of cytotoxicity at these concentrations. At a concentration of 3 μM, dehydrotumulosic acid caused a 160% increase in ALP activity, 24% higher than in the BMP-2 control. BMMs treated with dehydrotumulosic acid at concentrations between 10 and 100 μM showed a substantial 15–86% decrease in RANKL-induced inhibition activity compared to the control, with distinct patterns of RANKL inhibition and cytotoxicity observed at 10 μM. These findings suggest that the ethanol extract from the sclerotium of W. hoelen has potential to modulate bone-cell differentiation, while highlighting the possible benefits of dehydrotumulosic acid isolated from the dichloromethane fraction of W. hoelen for preventing and treating osteoporosis.

Priming 처리 및 온도 조건이 광나무 종자발아 및 유묘 생장 활력에 미치는 영향

진언주,배은지,이혁,손예원,최식원,이소라,윤준혁 경상대학교 농업생명과학연구원 2023 농업생명과학연구 Vol.57 No.3

본 연구는 국내 산림생명자원 중 광나무의 대량생산을 위한 기초연구로, Priming 처리에 따른 발아 효율성 및 유묘 생육특성에 미치는 영향을 구명하고자 수행하였다. 종자 Priming처리는 대조구, GA3 (10, 100, 200 ㎎·L-1), Ca (NO3)2 (50, 100, 200 mM), KNO3 (50, 100, 200 mM)를 24시간 처리하여 발아특성을 분석하였고, 순화재배에 따른 생육특성 및 활착률을 조사하여 수확량을 비교·분석하였다. 발아특성은 25℃·KNO3 100 mM에서 발아율이 유의적으로(p<0.001) 높았으며, 평균발아일수는 10.3~18.1일로 15℃에서 대체적으로 빠른 발아일수를 나타냈다(p<0.001). 발아속도 및 발아균일지수 또한 25℃·KNO3 100 mM에서 유의적으로 높은 값을 보였다(p<0.001). 생육특성의 경우 2 5℃·KNO3 100 mM 처리에서 유근 길이(5.1±2.4 ㎝), 초장(5.7±0.7 ㎝), 근장(16.6±2.0 ㎝), 건중량(0.079 g)이 유의적으로 가장 높게 나타났다 (p<0.001). 유묘활력지수 또한 25℃·KNO3 100 mM 처리에서 1418.3으로 높았으며, 가장 높은 수확량(16.8 g/㎡)을 나타냈다. 결과적으로 25℃, KNO3 100 mM 처리 시 실내발아에서 발아효율성을 높일 수 있었으며, 순화재배 시 우수한 유묘 생육을 보여 수확량을 증진시킬 수 있었다. This study is fundamental research for the mass production of Ligustrum japonicum Thunb., a domestic forest genetic resource. It aimed to investigate the effects of priming treatment on germination efficiency and seedling growth characteristics. The priming treatments included a control group, GA3 (10, 100, 200 mg/L-1), Ca (NO3)2 (50, 100, 200 mM), and KNO3 (50, 100, 200 mM), which were applied for 24 hours to analyze germination characteristics. The growth characteristics and rooting rate were investigated through cultivation, and the yield was compared and analyzed. The germination rate was significantly higher (p<0.001) at 25℃ with KNO3 100 mM, and the average germination days ranged from 10.3 to 18.1, indicating generally faster germination at 15℃ (p<0.001). The germination speed and germination performance index also showed significantly higher values at 25℃ with KNO3 100 mM (p<0.001). In terms of growth characteristics, the treatment of 25℃ with KNO3 100 mM resulted in significantly longer Radicle length (5.1±2.4 cm), Plant height (5.7±0.7 cm), Root length (16.6±2.0 cm), and dry weight (0.079 g) (p<0.001). The seedling vigor index was also highest at 25℃ with KNO3 100 mM (1418.3), and it exhibited the highest yield (16.8 g/m2). In conclusion, the germination efficiency could be enhanced by cultivating at 25℃ with KNO3 100 mM, and superior seedling growth was achieved through cultivation, leading to increased yield.

옥수수수염 추출물의 항산화효과 및 피부각질세포 보호효과

김현영,서우덕,서경혜,이미자,최식원,이광식,김선림,강현중 한국작물학회 2016 한국작물학회지 Vol.61 No.3

We investigated the antioxidative and protective effects of corn silk (Zea mays L.) ethanol extracts on human HaCaT cells and erythrocytes. The NICS-2 fraction, extracted from corn silk, exhibited favorable 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities with IC50valuesof13.3± 0.3 μg/mL and 14.2 ± 0.1 μg/mL when compared with those of α-tocopherol, a positive control, with IC50=10.4± 02.2 and 22.2 ± 3.6 μg/mL, respectively. In addition, we investigated skin protection effects of NICS extracts of corn silk in HaCaT keratinocytes. To investigate the pharmacological potential of NICS-1 and NICS-2 extracts of corn silk on UV-B-induced damage in HaCaT cells, we measured the activity of interleukin (IL) 1a. Our results showed that all the corn silk extracts inhibited the UV-B-induced activity of IL-1a. In particular, NICS-1 extracts of corn silk significantly suppressed IL-1a activity in a dose-dependent manner without inducing cytotoxicity. These results indicate that the ethanol extracts of corn silk (Zea mays L.) could function as natural cytoprotective agents and antioxidants in biological systems, particularly the skin exposed to UV radiation, by protecting cellular membrane against reactive oxygen species (ROS).

볶음 공정에 따른 발아 향미차의 향기성분 및 이화학적 특성

남산,권유리,조준현,서우덕,최식원,윤광섭 한국식품저장유통학회 2016 한국식품저장유통학회지 Vol.23 No.5

This study was performed to verify the possibility of manufacturing a germinated aromatic rice tea, which was roasted at 200, 250, and 300℃ each for 10, 20, and 30 min. The roasted aromatic rice was analysed physicochemical properties, sensory characteristics and aromatic compounds. The total polyphenol content and DPPH radical scavenging activities of the germinated aromatic rice increased as the roasting temperature and time increased. Total soluble solid contents, turbidity and browning index of the germinated aromatic rice tea also increased was the roasting temperature and roasting time increased. The pH did not change by roasting. The main aromatic components in roasted germinated aromatic rice tea were 2-methyl butanal, 3-methyl butanal, benzaldehyde and nonanal, which increased according to increasing temperature and time. However, those favorable aroma components were decreased at more than 300℃ of roasting temperature. In addition, methyl benzene, pentanol were increased which affect odor aroma. The sensory score of germinated aromatic rice tea also increased with high roasting temperature and time. However, aromatic rice roasted at a higher temperature (300℃) showed lower sensory score. Therefore roasting temperature and time must be controlled for manufactureing high quality of germinated aromatic rice tea, and the optimun roasting conditions were 250℃ and 30 min, which provide best physicochemical characteristics of aromatic rice tea.

Priming 처리조건이 광나무 종자의 발아 및 유묘 생육에 미치는 영향

진언주,윤준혁,이혁,최식원 경상국립대학교 농업생명과학연구원 2022 농업생명과학연구 Vol.56 No.6

The purpose of this study is to determine the proper pretreatment condition by comparing and analyzing the treatment time, type and density during priming treatment which is effective to germination and uniformity to improve the seedling effectiveness and productivity of Ligustrum japonicum. To obtain good quality seedling, after immersion treatment for 1 day, only sedimented seeds were used. For pretreatment, the test was conducted by treating GA3 (0, 10, 100, 200 ㎎·L-1) growth regulator and Ca(NO3)2 and KNO3 (0, 50, 100, 200 mM)inorganic salts according to priming treatment hour(24, 48 hours). As a result of analyzing germination feature, 24 hour priming treatment showed Germination rate, Germination speed, Germination performance index (p≤0.001) which are significantly higher than control group while 48 hour priming treatment showed the germination feature that is lower than control group. In particular, 24 hour priming and GA3 200 ㎎ treatment showed the highest effect (p≤0.001) such as Germination rate 44.0%, Germination speed 0.59%·day, Germination performance index 0.55. On the contrary, 48 hour priming, GA3 and Ca(NO3)2 200 mM treated specimen showed no germination. As a result of researching T/R ratio and Seedling Vitality Index targeting seedling from priming treated seeds, 24 hour priming GA3 200 ㎎ treatment which showed the highest germination rate, showed low T/R rate and reduction rate, and Seedling Vigor Index is 793.0 which is the highest (p≤0.001). In conclusion, the proper treatment agent that influences to the germination and growth of Ligustrum japonicum among priming type (GA3, Ca(NO3)2, KNO3) is judged to GA3 and proper treatment condition is found to be 200 ㎎, 24 hours. 본 연구는 광나무(Ligustrum japonicum) 종자의 파종 효율성 및 유묘 생산성을 증진시키기 위하여, 발아와 균일성에 효과가 있는 priming 처리 중 처리시간, 처리제 및 처리농도를 비교·분석하여 적절한 전처리 조건을 구명하고자 실시하였다. 광나무 종자는 충실 종자를 사용하기 위해1일간 침지처리 후 침강 종자만을 사용하였다. 종자 전처리는 priming 처리시간(24, 48시간)에 따라 GA3 (0, 10, 100, 200 ㎎·L-1) 생장조절제와Ca(NO3)2 및 KNO3 (0, 50, 100, 200 mM) 무기염류로 처리하여 시험을 수행하였다. 발아특성을 분석한 결과 priming 24시간 처리는 대조구보다유의적으로 높은 발아율, 발아속도, 발아균일지수를 보인 반면(p≤0.001), priming 48시간 처리 시 대조구보다 낮은 발아특성을 나타냈다. 특히, priming 24시간·GA3 200 ㎎ 처리에서 발아율 44.0%, 발아속도 0.59%·day, 발아균일지수 0.55으로 가장 높은 효과를 보였다(p≤0.001). 한편, priming 48시간·GA3 및 Ca(NO3)2 200 mM 처리구에서는 발아가 나타나지 않았다. priming 처리된 종자로부터 생장한 유묘들을 대상으로 T/R율및 유묘활력지수를 조사한 결과 발아율이 가장 높았던 priming 24시간·GA3 200 ㎎ 처리에서 낮은 T/R율과 수분감소율을 보였으며, 유묘 활력지수는793.0으로 가장 높게 나타났다(p≤0.001). 결과적으로 priming 종류(GA3, Ca(NO3)2, KNO3) 중 광나무 종자 발아 및 유묘의 생장에 영향을적정 처리제는 GA3로 판단되며, 적정 처리 조건은 200 ㎎, 24시간 처리로 나타났다.

옥수수수염 주정 추출물의 항당뇨 활성 평가

김현영,이미자,서우덕,최식원,김선림,정건호,장기창,강현중 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-