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재조합 Adeno-associated Virus 제작과 효율적 신조적 감염
최순연,박종구 啓明大學校 醫科大學 2001 계명의대학술지 Vol.20 No.2
본 실험에서는 신질환 질병모델의 in vivo 실험을 위해서 IL-10과 대조군으로 LacZ 유전자를 가진 재조합 AAV를 생산하였다. 또한, 신조직에서 유전자 운반 매개체로서의 재조합 AAV의 타당성을 확인하였다. 생산된 재조합 바이러스는, in vitro상에서 바이러스의 감염성과 유전자 발현 능력을 평가하였다. In vitro 실험에서 평가된 재조합 바이러스는 in vivo 실험에 사용되었다. 재조합 AAV를 8 주령 된 ICR 생쥐 왼쪽 신장의 실질조직으로 직접 주입한 후, 마취사 시켜 절취한 신장을 X-gal용액으로 염색하였다. 그 결과, 신장은 β-galactosidase 유전자 발현을 일개월간 관찰할 수 있었다. 또한, 광학현미경상에서 해부학적으로 조직의 염색된 부위를 관찰한 결과는 다음과 같았다. 주로 β-galactosidase 유전자로 형질 도입된 신조직은 세뇨관 상피세포와 보우만 주머니의 상피세포주위에 편중되어 나타났으나, 사구체, 모세혈관, 간질에서 단백질의 발현은 관찰할 수 없었다. 이러한 결과는 재조합 AAV가 신조직의 유전자 도입 매개체로서 이용 가능하며, 사이토카인 IL-10을 이용한 신질환치료에 효과적일 것이라 기대된다. Recombinant viral vectors based on the nonpathogenic parvovirus, adeno-associated virus (AAV), have a number of attractive features for the purpose of gene theraphy including the lack of cytotoxicity, the ability to transduce non-dividing cells and its long-term transgene expression. In this study, we studied if recombinant AAV (rAAV-LacZ and rAAV-hIL10) vectors could efficiently transduce cells both in vivo and in vitro. We initially examined AAV-mediated gene transfer in HeLaRC32 cells in vitro. Expression of transgenes in rAAV was evaluated by RT-PCR (rAAV-hIL10) and X-gal staining. We then studied if rAAV was effective in transducing renal tissue by direct injection of rAAV harboring the β-galactosidase gene (1× 10 exp (10)) physical particles/injected kidney) into the left kidney of mice. To assess gene transfer, the transduced renal tissue of mice was stained with X-gal. Based on visual assessment of X-gal staining, The expression of the β-galactosidase gene was mainly localized in tubular epithelial cells and Bowman's capsular epithelial cells. The expression of the β- galactosidase gene in the renal tissue was detected for more than one month after the transduction of rAAV. These results suggest that rAAV vectors can be effective for transgene expression in the renal tissue.
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병원조직에 팀제 도입이 팀직무만족과 팀조직 몰입에 미치는 영향
최순연(Soon Yeon Choi),남은우(Eun Woo Nam) 한국병원경영학회 2000 병원경영학회지 Vol.5 No.1
The introduction of a team system to any organization is usually to improve that organizations dynamics, by increasing the proper adaptation to rapidly changing environments, by reducing approval procedures, and by promoting power authorization. In this regard, this study was carried out in an attempt to determine (1) whether or not the introduction of a team system can be as effective in hospital organizations as it has been in business organizations, and (2) what behavior factors influence such effectiveness. For the current study, questionnaires were distributed to the employees of a university hospital in Pusan. Four hundred eighty three of them (94.7%) were retrieved and analyzed through statistical processes. The three types of variables that were found to influence team effectiveness were : (1) independent variables of team organization characteristics, such as communication, organizational atmosphere, leadership and team knowledge, (2) intervening variables of team organization characteristics, such as job autonomy, technological variety, task subjectivity, task feedback and task importance, and (3) the dependent variables of team level effectiveness, such as job satisfaction and commitment to team organization. The gathered data were processed by using the 6.12 version of the statistical program SAS (Statistical Analysis System), and the reliance coefficient of those measured variables was then evaluated as an average of 0.78. The influence of team level effectiveness was analyzed by using multiple regression analysis. These differences in effectiveness were then analyzed on the basis of related mean values, while the differences among demo-sociological characteristics were analyzed by using the dispersion analysis (ANOVA) and the t-test. The results of the study can be summarized by the following. First, regarding the relationship between team organization characteristics and team level effectiveness, all of the factors including communication, organizational atmosphere, leadership and team knowledge were found to have a significant influence on effectiveness. Second, the factors of team job characteristics, such as technological variety, task importance and job autonomy, had significant effects on job satisfaction and organizational commitment among the teams members. Team level effectiveness was generally high, while team job satisfaction was higher the teams organizational commitment. Third, regarding the demo-sociological characteristics, team level effectiveness was found to be higher among those who were older, who had more job experience, and who had a higher position. Comparing the differences among job types, the effectiveness was relatively higher in the fields of nursing and administration/management then in other fields. Further study should be done towards the setting of variables for characteristics of hospital organization, developing suitable methods of measurement, and researching individual level effectiveness.
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류황건,최순연,김명옥 고신대학교 영도발전연구소 2002 영도연구 Vol.4 No.-
The purpose of this study is to identify smoking behaviors of students. The subject of this study population was students and their parents who are attending at elementary schools in Yeongdo-Gu. 500 questionnaires were distributed and 497 questionnaires were returned. Finally, 493 responses were analyzed. The resulted were as follows: (1) 4.5% of respondents had experiences of smoking cigarettes. (2) The smoking rates of father was 63.2% and the smoking rate of grand father was 29.9%. (3) Most of the students had a lack of knowledge about the harm of smoking. Smoking students had lower level of knowledge then non-smoking students. (4) But the level of desirable attitudes about smoking was a high rate on students(85.1%) and their parents(81.2%) (5) The family history of smoking influenced on the family smoking rate significantly(p<.01). The fathers of students with smoking grand fathers showed highest smoking rate(81.8%).
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