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      • 실험용 야생계통 생쥐 고환의 Lectin 결합양상에 대한 비교연구

        최병태,최영현,조기진,이원호,조운복 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

        실험용 생쥐 BALB/c, ICR, C57BL/6N의 3계통과 진영 및 포천에서 채집한 한국산 야생 생쥐(Mus musculus subspecies) 고환의 복합당질 분포를 7가지 lectin(DBA, SBA, PNA, BSL-1, sWGA, UEA-1 및 Con A)으로 검색하였다. 정조세포와 일차정모세포의 세포표면에 PNA, sWGA 및 Con A가 반응을 나타내며 일차정모세포의 세포질에 PNA와 Con A가, 골지복합체부위에는 SBA와 sWGA가 반응을 나타내었다. 골지기에서 첨단체기에 이르는 정자세포의 첨단체에서는 SBA만 반응을 나타내었고, 세포질에서 PNA와 Con A가, 세포표면에서 PNA, sWGA 및 Con A가 반응을 나타내었다. 성숙기 정자세포의 미부와 Sertoli세포는 PNA, sWGA 및 Con A에 반응을 나타내고 세포질소적에는 이외에 SBA에도 반응을 나타내었다. 이상의 반응을 보면 정조세포에서는 미약하나 정자발생이 진행될 수록 그 반응정도가 강하게 나타났으며 SBA, PNA 및 sWGA의 반응이 현저하였다. 계통간 lectin 결합양상은 서로 유사하나 반응정도가 다소 상이한데 실험용 생쥐에서 대부분 일차정모세포의 골지복합체부위와 첨단체기 정자세포의 첨단체에 SBA반응을 보인 반면 야생형에서는 일부 세포만이 반응을 나타내었으며, 정자 세포의 세포표 면 PNA반응고 골지기와 두모기 첨단체의 SBA반응이 다른 계통에 비해 BALB/c가 강한 반응을 보였다. To localize glycoconjugates in the testis of laboratory (BALB/c, ICR and C57BL/6N)and wild mouse (Mus musculus subspecies) strains, seven different biotinylated lectins (DBA, SBA, PNA, BSL-1, sWGA, UEA-1 and Con A) were applied with ABC method. The cell surface of spermatogonia and primary spermatocytes showed an affinity for PNA, sWGA and Con A. Affinity for PNA and Con A was found in the cytoplasm, and SBA and sWGA in the Golgi region of primary spermatocytes. The developing spermatids in transition from Golgi to acrosome phase of spermiogenesis, affinity for SBA was detected in acrosome, PNA and Con A in cytoplasm, and PNA, sWGA and Con A in cell surface. However, affinity for PNA, sWGA and Con A was observed only in the tail of spermatids on maturation phase of spermiogenesis, and additional affinity for SBA revealed in the cytoplasmic droplet. Affinity for PNA, sWGA and Con A showed in the cytoplasmic process of Sertoli cells. And affinity for lectins continued to increase according to development from spermatogonia to spermatid, especially, SBA, PNA and sWGA elicited stronger affinity than the others. Between labolatory and wild mouse strains, affinity for lectins showed a similar pattern except SBA and PNA. Most Golgi region of primary spermatocytes and acrosome of spermatid on acrosome phase showed an affinity for SBA in laboratory mouse strains, however, in wild strains, this affinity was present in a few cells. More intense both affinity for PNA in cell surface of developing spermatid and SBA in acrosome of spermatid on golgi and cap phase revealed in BALB/c strain as compared with the others.

      • KCI등재

        상산의 $NF-{\kappa}B$ 활성억제작용과 $IKK{\gamma}$의 연관성 연구

        최병태,이용태,황장선,문혜인,이경수,안원근,김동완,Choi, Byung-Tae,Lee, Yong-Tae,Hwang, Jang-Sun,Moon, Hae-In,Lee, Kyung-Soo,An, Won-Gun,Kim, Dong-Wan 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6

        Activation of $NF-{\kappa}B$ is known to be a trigger of various cellular disorders including inflammatory and autoimmune diseases such as rheumatoid arthritis. Numerous approaches are ongoing within laboratories to identify potential therapeutic agents which inhibit the $NF-{\kappa}B$ activation. In this study, we have tested the inhibitory effects of five traditional medicines on the activation of $NF-{\kappa}B$ by NIK. Among three medicines which exhibited inhibitory effect on the expression of $NF-{\kappa}B$ repoter plasmid, we investigated further the inhibitory mechanism of Dichroa febrifuga in connection with IKKY activity. Wild type $IKK{\gamma}$ inhibited the $NF-{\kappa}B$ activation by NIK but the C-terminal deletion mutant of IKKY did not show the inhibitory effect, indicating that the C-terminal leucine zipper domain of $NF-{\kappa}B$ is important for the inhibition of $NF-{\kappa}B$ activation. The water extract of Dichroa febrifuga(DFE) also strongly inhibited the $NF-{\kappa}B$ activation by NIK. The inhibitory activity of DFE appeared to be independent of the expression of $IKK{\gamma}$, suggesting that the pathways of inhibition by Dichroa febrifuga and $IKK{\gamma}$ are different. Our results suggest that Dichroa febrifuga can be used as a medicine for inhibition of the $NF-{\kappa}B$ activation in a wide range of cells without relation to the expression of $IKK{\gamma}$.

      • 발생기 랫드의 십이지장점막 점액질에 대한 조직화학적 연구

        최병태,조운복 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

        The Changes of mucosubstances in the duodenal mucosa of developing rats were investigated by conventional methods. On the 15 and 17 day-old fetal rat, neutral mucosubstances were observed in the stratified epithelium of the duodenum, whereas both neutral mucosubstances and sialomucins were demonstrated in the oligomucous cells on 17 day-old fetal rat. 'The brush border contained neutral mucosubstances and sialomucins from 19 day-old fetal rat, and thereafter changed the properties as a mixture of neutral mucosubstances, sialomucins and weak sulfomucins. In the goblet cells, most cells showed a mixture of neutral mucosubstances and strong sulfomucins front 19 day- old fetal rat, but a few cells contained neutral ones only. The amount of neutral and acid mucosubstances increased in the goblet cells of both villi and crypt during the suckling period. However acid mucosubstances increased in the goblet cells of crypt during the weanling period. 'Ihe Brunner's gland contained neutral mucosubstances only from 21 day-old fetal rat, but from the end of suckling period, a few cells located deep gland region contained additional strong sulfomucins.

      • KCI등재
      • KCI등재

        도덕적 문제해결 능력과 도덕교육

        최병태 충남대학교 교육발전연구소 2002 교육연구논총 Vol.23 No.2

        이 글에서는 도덕교육의 핵심적인 과제는 도덕적 문제해결 능력을 기르는 것이라고 보는 관점을 비판적으로 검토하였다. 도덕교육에 있어서는 "문제"의 성격도 불분명하고, 문제를 "해결"한다는 것이 어떤 것인지도 분명하지 않기 때문에 도덕교육의 핵심적인 과제를 도덕적 문제를 해결할 능력을 기르는 것으로 해석하는 입장은 잘못된 것이라는 점을 밝혔다. 도덕교육을 도덕적 문제해결이라는 시각에서 보는 관점을 비판적으로 검토한 후에 이러한 접근에 대한 대안을 모색해 보았다. 도덕적 문제해결 능력 기르기에 대한 대안으로 제시된, 올바른 의미에서의 도덕교육의 과제는 도덕적 감수성 기르기, 규범의 내면화, 바람직한 성품의 함양 등 세 가지이다.

      • 消費者 需要의 決定要因에 관한 理論的 考察

        崔炳太 대구산업정보대학 1990 논문집 Vol.4 No.-

        A study on the determinants of consumer demand is generally dealt with the traditional consumer theory from the viewpoints of micro-economics. But in realty consumer demand depends on many factors besides price factor on basis of micro-economics. Therefore, the purpose of the present study is to reestablish the system of consumer behavior by analyzing on the determinants of consumer demand from the viewpoints of micro-economics, macro-economics, and managerial administration. As a result this study can be summarized as follows: First, from the viewpoints of micro-economics on basis of price factor, consumer demand depends on mutual relations of consumers, namely consumers actually behaviors on the bandwagon effect, snob effect, and veblen effect. Second, from the viewpoints of macro-economics on basis of income factor, consumption is determined by absolute income, relative income, permanent income, long life income and so forth. Third, from the viewpoints of manageiral administration, a study on the consumer behavior can be classified by social, cultural factor and individual, psychological factor. From the above mentioned facts, finally the determinants of consumption can't be discussed with the social economic situation. Therefore the determinants of consumption must be analyzed completely from the viewpoints of social science in future. In additions the determinants of consumption suggested in this present study must be tested empirically and measurements for this purpose must be developed.

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