효소와 미생물의 복합 처리에 의한 두유박 단백질소재의 제조

채희정,인만진,이종대 ( Hee Jeong Chae,Man Jin In,Jong Dae Lee ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.1

The effects of soymilk residues solubilization by cellulase, protease, koji and yeast were investigated on dry matter and protein yields, amino acid and organic acid contents. Co-treatment of soymilk residues by cellulase and protease gave high dry matter yield and protein yield. Koji treatment followed by yeast fermentation was effective for increasing organic acid content and producing soy sauce-like taste and odor. Organic acid content of fermented hydrolysates was improved by cellulase treatment. Protease treatment rather than koji treatment gave high amino acid content, and cellulase treatment seemed to have little effect on increasing free amino acid content. In sensory evaluation, koji-treated hydrolysate showed higher overall acceptance than other hydrolysates, however it showed lower overall acceptance than commercial fermented soy sauce.

단백소재 첨가물로서의 효소분해 대두 단백질의 특성

채희정,안만진,김민홍 ( Hee Jeong Chae,Man Jin In,Min Hong Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.5

Enzymatically hydrolyzed vegetable protein (eHVP) was produced from soy protein using proteases, and the physicochemical properties were examined. Soy protein hydrolysate of 60% protein and 50% degree of hydrolysis was useful for the base of savory ingredients. The Maillard-reacted and flavoring compound-added hydrolysate had improved flavor. It was for enzymatically hydrolyzed soy sauces and dehydrated seasonings. ISP hydrolysate of low molecular weight (MW∼250) and high protein content (85%) was suitable for special uses such as infant diets, sports nutrition, and medical diets. The eHVP gave no limitation of dosage in the formulation as a flavor enhancer. The byproduct of protein hydrolysis was found to have high content of fiber (21%) and to have potential for the use as dietary fiber or bulking agents.

Colletotrichum orbiculare에 대한 길항세균 Pseudomonas aurantiaca YC4963의 분리 동정 및 항균물질 Phenazine-1-carboxylic acid의 생산

채희정,김루미,문석식,안종웅,정영륜,Chae Hee-Jung,Kim Rumi,Moon Surk-Sik,Ahn Jong-Woong,Chung Young-Ryun 한국미생물학회 2004 미생물학회지 Vol.40 No.4

경남 지역의 근권 토양 및 식물뿌리 에서 다양한 길항세균을 분리하여 오이 탄저병원균 Colletotrichum orbiculare에 대한 길항효과를 조사하였다. 그중 국화과에 속하는 털진득찰 (Siegesbeckia pubescens Makino)뿌리에서 분리된 YC4963 균주가 병원균의 균사 생장에 대한 억제 능력이 가장 우수하였으며, 배양 상등액을 이용한 in vitro 실험에서도 C. orbiculare의 발아관 형성과 균사 생장을 억제하였다. 이 균은 Gram음성과 양성세균에 대 해서도 억제 능력이 있었으며, Botrytis cinerea, Fusalium oxysporum, Rhizoctonia solani 등의 다양한 식물병원균에 대해서도 억제 능력이 좋았다. 이 균주의 형태, 생리$\cdot$화학적 특성과 분자생물학적 특성을 조사한 결과 Pseudomonas aurantiaca로 동정되었다. 이 길항 세균이 분비하는 항생물질의 구조결정을 위하여 대량 배양 후 물질 분리와 여러 종류의 크로마토그래피를 수행하였다. 분리 정제하여 얻은 순수 물질은 노란색 바늘 모양의 결정체이었고, 질량 분석, FT-IR spectrum분석 및 NMP spectrum 분석을 바탕으로 구조를 추정한 결과 phenazine-1-carboxylic acid로 확인되었다. 이 항생물질의 활성을 조사하기 위하여 농도를 달리하여 C. orbiculare의 발아관 및 부착기 형성을 조사한 결과, 처리 18시간 후 발아관 생장은 103 ${\mu}m$로 대조구의 798 ${\mu}m$보다 8배 정도 억제되었으나 발아관과 부착기 형성비율은 큰 차이가 없었다. P. aurantiaca에 의한 phenazine-1-carboxylic acid의 생산과 이 항생물질에 의한 탄저병군의 억제효과는 본 연구에서 처음 보고되는 것이다.$생산 유전자가 전달되는 것을 확인하였다. Random amplified polymorphic DNA와 pulsed field gel electrophoresis분석을 사용하여 genomic DNA에 대한 유전형을 분석한 결과 균주간의 유전적 연관성은 매우 낮은 것으로 나타나 한 병원에서 발견되는 균주는 clonal spread에 의한 것이라는 일반적인 보고와 다른 결과를 얻었다., 체외순환을 시작한 이후부터는 2군에서 지속적으로 더 높은 경향이 있었으며(1군 $48.5\~64$ mL/min100 g, 2군 $65.8\~88.3$ mL/min/100 g), 특히 30분에서의 측정값은 통계적으로 유의한 차이를 보였다(1군$47.5{\pm}18.3\;mL/min100\;g,$ 2군$83.4{\pm}28.5\;mL/min100\;g,\;p=0.026$). 혈액 뇨질산, 크레아티닌, 그리고 혈장 용혈헤모글로빈의 변화는 두 군간에 차이가 없었다. 결론: 일정한 펌프 혈류 조건에서 박동성 혈류의 평균 혈압이 더 높다는 것은, 비박동성 혈류보다 조직관류압(Tissue Perfusion Pressure) 측면에서 우수하여 말초장기의 조직관류 효과에 유리한 요인이라고 볼 수 있다. 본 연구를 토대로 장시간의 체외순환에서는 신장기능을 대표하는 수치들에도 영향을 미칠 수 있으리라 예상되며, 신장 이외에 다른 주요 장기에 미치는 영향에 대한 연구를 더 진행할 필요가 있을 것으로 생각한다.예측 인자가 될 수 있을 것으로 생각된다.있을 것으로 판단된다.%$의 무기물질(Zeolite)이 첨가되어진 Modified California putting green system이 최적의 putting green 조건과 우수한 Bentgrass 잔디품질을 4년 동안 유지하였음을 이 실험을 통해 조사되어졌다. A bacterial strain YC4963 with antifungal activity against Colletotrichum orbiculare, a causal organism of cucumber anthracnose was isolated from the rhizosphere soil of Siegesbeckia pubescens Makino in Korea. Based on physiological and biochemical characteristics and 16S ribosomal DNA sequence analysis, the bac­terial strain was identified as Pseudomonas aurantiaca. The bacteria also inhibited mycelial growth of several plant fungal pathogens such as Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani on PDA and 0.1 TSA media. The antifungal activity was found from the culture filtrate of this isolate and the active compound was quantitatively bound to XAD adsorption resin. The antibiotic compound was purified and identified as phenazine-l-carboxylic acid on the basis of combined spectral and chemical analyses data. This is the first report on the production of phenazine-l-carboxylic acid by Pseudomonas aurantiaca.

발효 및 식품 공장의 용매 회수용 증류탑의 기본 설계

채희정,인만진,김민홍 ( Hee Jeong Chae,Man Jin In,Min Hong Kim ) 한국산업식품공학회 1997 산업 식품공학 Vol.1 No.2

효소반응 모델식에서의 매개변수 추정

채희정(Hee Jeong Chae),김지현(Ji Heyon Kim),차형준(Hyung Joon Cha),유영제(Young Je Yoo) 한국생물공학회 1990 KSBB Journal Vol.5 No.2

실크의 가용화 조건이 효소분해 실크 펩타이드의 분자량 분포에 미치는 영향

채희정(Hee Jeong Chae),인만진(Man-Jin In),김의용(Eui Yong Kim) 한국생물공학회 1998 KSBB Journal Vol.13 No.1

사이버 명예훼손에 대한 현행법상 규제와 그 한계

채희정(Chae Hee jeong) 충북대학교 법학연구소 2014 과학기술과 법 Vol.5 No.1

In modern society, the communication by internet plays very important roles in many aspects. Although there are many positive aspects, there are many negative aspects in internet. So we can secure diversity, liberty in society, consensus, solidarity and democratic atmosphere. This article examines the online defamation law and criticizes the recent proposals for its reform in Korea. Recently, as internet is becoming common method of communication, we can experience cyber defamation on web space so often, and be victim of cyber defamation. so because of this phenomenon and serious results of defamation victimization on cyber space, there are many kinds of opinions that new responsive means have to be taken to this problem, for example, system to open real name about internet users,more severe criminal punitive methods. Also an internet total information provider, as an intermediary of uploadings on the Internet, may be liable in torts jointly with the person directly uploading it. In case where requirements of recognition and avoidance possibility are fulfilled, the above provider is liable in torts by non-action. The Supreme Court, in this case, decides legal ground and requirements for which the above provider burdens tort liability about uploadings on the Internet containing defamatory contents. The Majority Opinion holds that in case where the recognition of an existence and unlawfulness of uploadings appears obvious; and its management and control is feasible technologically and financially, the above provider has duty of care to deleting the uploadings. But the key point to solve these serious problems is that we must find solution to secure liberty of speech or expression, simultaneously to secure safety of cyber space, because two aspects has same important meaning with us.

고체상 추출에 의한 l,3-dichloro-2-propanol과 3-chloro-l,2-propanecnol의 분석

채희정(Hee Jeong Chae),인만진(Man-Jin In),김민홍(Min-Hong Kim),한금수(Keum-Soo Han) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.1

풍속과 풍향이 미세먼지농도에 미치는 영향

채희정(Hee-jeong Chae) 대한환경위생공학회 2009 대한환경위생공학회지 Vol.24 No.3

The study has analyzed impacts and intensity of weather that affect PM₁? concentration based on PM₁? forecast conducted by the city of Seoul in order to identify ways to improve the accuracy of PM₁? forecast. Variables that influence PM₁? concentration include not only velocity and direction of the wind and rainfalls, but also those including secondary particulate matter, which were identified to greatly influence the concentration in complicated manner as well. In addition, same variables were found to have different impacts depending on seasons and conditions of other variables. The study found out that improving accuracy of PM₁? concentration forecast face some limits as it is greatly influenced by the weather. As an estimation, this study assumed that basic research units and artificially estimated pollutant emissions, study on mechanisms of secondary particulate matter productions, observatory compliment, and enhanced forecaster's expertise are needed for better forecast.

광범위큰B세포림프종에 대한 키메릭항원수용체T세포 (Chimeric Antigen Receptor T-cells) 치료

채희정 ( Heejung Chae ),윤덕현 ( Dok Hyun Yoon ) 대한내과학회 2019 대한내과학회지 Vol.94 No.2

CD19 chimeric antigen receptor T-cell (CAR-T) therapy, a genetically engineered cell therapy, showed unprecedented efficacy in the treatment of relapsed or refractory diffuse large B-cell lymphoma. Two agents, axicabtagene ciloleucel and tisagenlecleucel, were approved by the Food and Drug Administration in 2017. However, CAR-T therapy is a treatment with complex logistics and high costs, as well as inherent adverse events, including cytokine-release syndrome and neurotoxicity. In addition, predictive biomarkers for efficacy and toxicity are lacking. Industry-academy cooperation is urgently required to develop CAR-T therapy that is effective, safe, and affordable for patients in Korea. (Korean J Med 2019;94:152-158)