동종 말초혈액 조혈모세포이식에서 이식된 CD34+ 세포량에 따른 임상적 결과

채의수,전석봉,성우진,임종우,김동환,김종광,박태인,손상균,서장수,이규보 大韓血液學會 2003 大韓血液學會誌 Vol.38 No.1

동종 말초혈액 조혈모세포를 이용한 다양한 임상적용에 관한 단일병원 경험보고

채의수,손상균,성우진,서광운,박성원,김종광,정진태,이난영,서장수,이규보 경북대학교 병원 2002 경북대학교병원의학연구소논문집 Vol.6 No.1

배경:동종 말초혈액 조혈모세포 이식은 골수이식에 비해 많은 양의 조혈모세포 및 T―림프구 이식으로 인해 빠른 회복이 가능하며 강력한 이식편대종양반응이 유도될 수 있는 장점이 있어 이를 잘 응용한다면 악성 혈액질환에서 치료율을 높일 수 있을 것으로 사료된다.본 연구에서는 동종 말초혈액 조혈모세포 이식의 장점을 이용한 다양한 임상적용을 소개하고자 한다. 방법:경북대학교병원 조혈모세포 이식센터에서 동종 말초혈액 조혈모세포 이식을 시행한 증례 중에서 동종 골수이식수술에 비해 말초혈액 조혈모세포 이식이 유리할 것으로 생각디는 재발의 가능서이 높은 악성질환에서의 더욱 강력한 이식편대종양반응 유도를 위한 동종 말초혈액 조혈모세포를 이용한 예방적 공여자 림프구주입 등의 임상적용을 시도한 증례 및 결과를 분석하였다. 결과:17례의 재발의 가능성이 높은 고위험군의 악성 혈액질환을 대상으로 한 동종 말초혈액 조혈모세포 이식에서 90일 이내의 치료와 관련된 사망률은 23.5%이었고 중앙 추적기간 563일(범위 120∼926일)간의 무병생존율은 35.3%이었다.CSF로 가동화한 말초혈액 조혈모세포를 추가적으로 수혈받은 환자 7명 중 4명(57.1%)이 중앙 추적기간 538일(범위120∼926일)째에 완전관해 상태로 생존 중에 있다.그 외 말초혈액 조혈모세포를 아용한 미니 이식,동종 말초혈액 조혈모세포를 이용한 예방적 stepwise dose-increment 공여자 림프구주입술 등을 시행한 증례에서 장기 생존의 결과를 보여주고 있다. 결론:동종 말초혈액 조혈모세포 이식은 많은 양의 조혈모세포 및 T-림프구의함유로 인해 높은 생착률과 빠른 회복,강력한 이식편대종양반응 등이 유도되는 장점이 있으므로 기존의 골수이식술에 비해 보다 광범위한 임상적용이 가능하리라 사료된다. Background: It is apparent that more stem cells can be harvested by mobilization treatment with recombinant human G-CSF and/or GM-CSF from normal healthy donors in allogeneic peripheral biood stem celltransplantation(allo-PBSCT) compared to allogeneic bone marrow transplantation(allo-BMT).It is also known to be more effective in inducing the graft-vs-tumor affects than allogeneic BMT because of higher T cell content. Methods: A variety of clinical applications with allo-PBSCT was done for patients with hematoligical malignancies with a high risk of relapse in sigle transplantation center. We reported the preliminary results on trial of allo-PBSCT followed by planned prophyactic G-and/or GM-CSF primed donor lymphyocyte infusion additionally reserved at harvest in hematological malignancies with a high risk of relapse and also presented the successful trial of non-myeloablative transplantation for old aged AML paient in 4th complete remission and cases with 2nd transplantation with allo-PBSCs. Results: Seventeen patients with hematological malignancies with a high risk of relapse were enrolled in trial of allo-PBSCT followed by prophylactic donor lymphocyte infysion.All patients received allogeneic PBSCT from HLA- matched sibling donor.Aeven out of 17 patients recived additional PBSCs with a median number of CD3+ CELLS OF 5.0×10^7/kg(range,3.0 to 9.9×10^7/kg), between day 41 and day 120.Four surving patients(4/7) were free of disease when last assessed(median follow-up duration, 538 days), but were suffering from chronic GVHD(1 limited and 3 extensive). A 56 year old acute myeloid leukemia patient in the 4th complete remission was successfully treated with allo-PBSCT with non-myeloablative conditioning regimen.One of 2 patients who received second transplantation with allo-PBSCT has shown a long term disease free survival. Conclusion: A merit of allo-PBSCT would allow us to design a variety of clinical applications. Allo-PBSCT might be preferable in special clinical setting such as non-myeloablative transplantation, second transplantation,or the situation in need of the strong GVL effect. And also CSF-primed PBSCs can be used for the purpose of donor lymphocyte infusion.(korean J Hematol 2001;36:214~222)

Association of Vascular Endothelial Growth Factor Gene Polymorphisms with Susceptibility and Clinicopathologic Characteristics of Colorectal Cancer

채의수,김종광,손상균,Yoon Young Cho,Byung Min Ahn,문준호,Seoung Woo Jeon,박재용,In Taek Lee,최규석,Soo Han Jun 대한의학회 2008 Journal of Korean medical science Vol.23 No.3

Since vascular endothelial growth factor (VEGF) is known to be a potent pro-angiogenic factor, we evaluated the potential association of two VEGF gene polymorphisms (-634G>C and 936C>T) with the susceptibility and the clinicopathologic characteristics of colorectal cancer (CRC). The VEGF genotypes were determined using fresh colorectal tissue from 465 patients who had undergone a surgical resection and peripheral blood lymphocytes from 413 healthy controls by PCR/DHPLC assay. For the -634G>C polymorphism, the -634 GC or CC genotype was associated with a decreased risk of CRC (odds ratio [OR], 0.62; p=0.001) as a dominant model of C allele, whereas the 936 TT genotype correlated with advanced stage/ metastasis, a high serum level of CA19-9, and an higher grade in patients with CRC. In the haplotype analyses, haplotype -634C/936C and -634G/936T were associated with a decreased susceptibility of CRC (OR, 0.53 and 0.56; p<0.001, respectively). These observations imply that the VEGF gene polymorphisms may be associated with the susceptibility or clinicopathologic features of CRC. However, further studies of other VEGF sequence variants and their biological functions are needed to understand the role of the VEGF gene polymorphisms in the development and progression of CRC.

동종 말초혈액 조혈모세포이식에서 이식된 CD34+ 세포량에 따른 임상적 결과

채의수,전석봉,성우진,임종우,김동환,김종광,박태인,손상균,서장수,이규보 대한혈액학회 2003 Blood Research Vol.38 No.1

헤스스테릴 을 함유한 제대혈의 압축을 이용한 적혈구 제거효과 및 단핵구 수거율

정진태,채의수,박성원,백진호,김동환,박소향,손상균,서장수,이재태,이규보 大韓輸血學會 1999 大韓輸血學會誌 Vol.10 No.2

잎들깨에서 차먼지응애에 대한 천연 피레스럼 방제효과

서윤경,채의수,안승원,최용석 한국응용곤충학회 2020 한국응용곤충학회지 Vol.59 No.4

시설잎들깨에 발생하는 차먼지응애를 친환경적으로 방제하고자 피레스럼(pyrethrum)의 농도별 방제효과를 조사하였다. 2%, 4%, 6% 피레스럼 1,000배 희석액에 대한 차먼지응애 약·성충의 생충률은 약제 살포 3일 후 각각 58.1%, 27.5%, 22.7% 였으며, 5일 후 각각 73.4%, 37.3%, 30.6% 였다. 차먼지응애에 대하여 방제효과가 우수했던 6% 피레스럼 1,000배액와 화학약제인 밀베멕틴(milbemectin EC 2%) 1,000 배액을 이용하여 약제 살포 후 차먼지응애에 대한 약·성충 밀도를 지름 1cm 잎디스크 내에서 조사한 결과, 6% 피레스럼을 차먼지응애의 발생이 예측되는 시기인 5월 10일부터 10일 간격으로 사용한 곳에서 밀도가 낮게 유지된 반면 차먼지응애의 피해가 확인된 후 화학약제를 사용한 곳에 서는 1주 간격 1~2회의 살포로는 차먼지응애의 밀도를 낮출 수 없었으며 1주 간격 3회 살포 이후 밀도가 낮아졌다. 따라서, 시설잎들깨에서 효과 적으로 차먼지응애를 방제하기 위해서는 발생이 예측되는 초기부터 방제제를 사용하는 것이 효과적이다. We investigated an ecologically-sensitive method to control Polyphagotarsonemus latus in a green perilla polyvinyl house using concentrated pyrethrum. The survival rates of Polyphagotarsonemus latus nymphs and adults to 2%, 4%, and 6% pyrethrum 3 days after spraying were 58.1%, 27.5%, and 22.7% respectively, and 73.4%, 37.3%, and 30.6% at 5 days after spraying. These results show that the most effective control occurred using 6% pyrethrum. To investigating the densities of P. latus on a 1 cm diameter leaf-disk of green perilla, we sprayed the leaf every 10 days from May 10 with 6% pyrethrum 1,000 times and milbemectin EC 2% 1,000 times. The period chosen was one where the incidence of P. latus could be predicted. The density was kept low during the treatment period. However, if leaf damage had already occurred from P. latus, the density could not be decreased by spraying 1 or 2 times each week where milbemectin EC 2% was used, but the population could be reduced when sprayed 3 times. Therefore, in order to effectively control P. latus in green perilla greenhouses, it is important to begin treatment at an early stage when P. latus are first observed.

Dexamethasone versus betamethasone:comparison of antenatal corticosteroids on neonatal outcome in preterm newborn

조민경,채의수,주다혜,이상욱,이상수,손우석 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: The aim of this study was to assess effectiveness of antenatal steroids and to compare the outcomes of the effects of dexamethasone and betamethasone in reducing perinatal complications among preterm infants. 방법: A retrospective cohort analysis of premature single infants born at a gestational age between 24 weeks and 33 weeks 6 days in our perinatal center from January 2006 through February 2015. 199 premature infants were included; who received full dose of steroid and none, fully used dexamethasone and betamethason, and non use of steroid and insufficient dose of steroid use. We excluded premature infants with clinical chorioamnionitis, fetal structural and chromosomal abnormalities, prior antenatal steroid exposure, and steroid use for other indications.(Table1) 결과: Antenatal corticosteroid therapy reduced the use of mechanical ventilation, the need for supplementary oxygen, and the need for exogenous surfactant in neonates born. Corticosteroid treatment seemed to benefit the respiratory distress syndrome (RDS; p=0.001). There were less cases of ROP with corticosteroid treatment (p<0.05). There was no statistically significant difference between the dexamethasone group and the betamethasone group.(Table 2) But, the length of ventilator use (hours) was longer in the dexamethasone group than the betamethasone group (Fig.1). Comparison between the insufficient dose group and the nonuse group showed no statistical significance either.(Table 3) 결론: Antenatal corticosteroids were effective in reducing major complications among the premature newborns in the population studied. We found that two major kinds of antenatal corticosteroids have similar outcomes. Comparison between the insufficient dose group and the nonuse group showed no statistical significance. Therefore their use and treatment duration should be sufficient enough to provide the full dose of steroid. Our data demonstrate that dexamethasone and betamethasone are equally acceptable.

Decreased Progesterone Receptor B/A Ratio in Endometrial Cells by Tumor Necrosis Factor-Alpha and Peritoneal Fluid from Patients with Endometriosis

김성훈,채의수,민진영,임효진,오영상,박소윤,채희동,김정훈,강병문 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.6

Purpose: Progesterone resistance is thought to be a major factor that contributes to progression of endometriosis. However, it is not clear what causes progesterone resistance in endometriosis. This study aimed to assess whether cytokines or peritoneal fluid can affect progesterone receptor (PR) expression in endometrial cells and to verify whether PR expression is reduced in endometriosis. Materials and Methods: The PR-B/A ratio was measured via real-time polymerase chain reaction after in vitro culture, in which endometrialcells were treated with either tumor necrosis factor-alpha (TNF-α), interleukin-1 beta, or peritoneal fluid obtained from women with advanced-stage endometriosis. Immunohistochemistry was performed to compare PR-B expression between eutopic and ectopic endometrial tissues from women with and without advanced-stage endometriosis. Results: The PR-B/A ratio was significantly decreased by treatment with either TNF-α (p=0.011) or peritoneal fluid from women with advanced-stage endometriosis (p=0.027). Immunoreactivity of PR-B expression was significantly lower during the secretory phase than during the proliferative phase in endometrial tissues from control subjects (p<0.001). PR-B expression was significantlyreduced in the eutopic endometrium (p=0.031) and ovarian endometrioma (p=0.036) from women with advanced-stage endometriosis compared with eutopic endometrium tissues from control subjects. Conclusion: Progesterone resistance in endometriosis may be caused by proinflammatory conditions in the pelvic peritoneal microenvironment.

포스타 전시 발표 : 혈액종양 ; 동종 말초혈액 조혈모세포이식에서의 거대세포바이러스 감염 및 선제치료 효과

성우진,채의수,서광운,박성원,김종광,정진태,손상균,서장수,이규보 대한내과학회 2001 대한내과학회 추계학술발표논문집 Vol.61 No.-

천연 피레스린에 대한 차먼지응애에 살충효과

서윤경,채의수,안승원,최용석 한국환경과학회 2020 한국환경과학회 학술발표회 발표논문집 Vol.2020 No.11