http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
체세포복제 소 배반포의 Oct-4 발현과 DNA 메틸화 변화
차병현,최정상,황성수,정학재,임기순,양병철,김명직,조재현,성환후,고응규 韓國受精卵移植學會 2008 한국동물생명공학회지 Vol.23 No.3
DNA methylation is one of the reasons for poor survival of clone animals. The OCT-4 gene is essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that the 5'-promoter region of Oct-4 gene was a target of DNA methylation and the methylation status was changed variously during embryonic development in bovine. The study conducted to examine the expression and methylation pattern of tissue-dependent differentially methylated region (T-DMR) of Oct-4 gene in bovine somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF) blastocysts. The Oct-4 gene expression was evaluated by RT-PCR and fluorescence immunocytochemistry. The methylation pattern of T-DMR was analyzed using restriction mapping and bisulfite sequencing methods. The Oct-4 transcripts were highly expressed in IVF, while they were not expressed in SCNT. The Oct-4 protein was not detected or expressed at very low level in SCNT, the intensity of Oct-4 protein, however, was strong in IVF. On the other hand, the T-DMR of Oct-4 gene was hypermethylated in SCNT compared to that of IVF. These results suggested that expression and the failure of demethylation of Oct-4 gene was closely associated with incomplete development of SCNT embryos.
Drug repositioning of tauroursodeoxycholic acid for bone tissue regeneration
이수홍,차병현,김병주,( Arai Yoshie ),김병수,한인보 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Bone tissue engineering from bone morphogenetic protein (BMP) and stem cells has been developed with various strategies for enhancement of therapeutic effect. Our recent study found that chemical chaperone tauroursodeoxycholic acid (TUDCA) significantly reduces adipogenesis of mesenchymal stem cells (MSCs). In present study, we examine whether TUDCA as an alternative drug of BMP is able to activate bone tissue regeneration by reducing adipose tissue. It was observed that TUDCA treatment was able to promote in vivo bone tissue regeneration at calvarial defect model and spinal fusion model. In addition, TUDCA treatment significantly decreases apoptosis and inflammatory response in vivo as well as in vitro. The results indicate that TUDCA plays a critical role in not only enhancing in vivo bone regeneration also reducing adipose tissue at implantation site, therefore, would be a potential alternative drug for bone tissue regeneration in pharmacologic and therapeutic applications.
중간엽줄기세포 부착 및 증식을 위한 poly-l-lysine 도입 PLGA 나노섬유 제조 및 분석
강광식,차병현,박광숙,김희천,박한수,박귀덕,이수홍 한국생체재료학회 2013 생체재료학회지 Vol.17 No.2
Cell-substrate interaction is fundamentally essential for phenotypic maintenance and multipotency of stem cells. To regulate the cell-substrate interaction, many researchers have tried to mimic the native cellular environment by introducing physical and chemical factors. Electrospinning technique is a useful tool to produce nanofibers with various diameters, porosities and structures like the native cellular physical environment. In electrospinning technique, however,most synthetic biodegradable polymers such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA) and poly(lactic acid-co-glycolic acid) (PLGA) show low adhesion and proliferation of cells on them. Recently, poly-l-lysine (PLL) has been reported as a common coating material for the improvement of cell adhesion. In this study, we hypothesized that PLL introduction in PLGA nanofiber would enhance the adhesion and proliferation of human adipose-derived stem cells (hASCs). To aim this, we fabricated PLGA/PLL nanofiber by electrospinning and evaluated cellular behavior of hASCs on it. PLGA/PLL nanofiber significantly promoted the attachment and proliferation of hASCs without cytotoxicity. Thus, PLGA/PLL nanofiber would be a useful scaffold to enhance adhesion and proliferation of stem cells and presumably to regulate differentiation of them in tissue engineering field.
김진수,권대기,차병현,문보경,정영헌,한인보,박한수,이수홍 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.60 No.-
We used porous membranes for co-culturing adipose-derived stem cells (ASCs) with nucleus pulposus cells (NPCs) to investigate whether repeated co-cultures restored the chondrogenic properties of degenerative NPCs (dNPCs). Human dNPCs were co-cultured with ASCs for 1 week and co-cultured again with new ASCs for 1 week. The expression levels of chondrogenic genes and GAGs were significantly higher in second co-cultured dNPCs than those in the first co-cultured dNPCs and in dNPCs cultured alone, respectively. After six weeks of in vivo transplantation, compared to first co-cultured dNPCs, second co-cultured dNPCs showed a significantly higher cartilage tissue formation.
신재성,차용호,차병현 한국광학회 2019 Current Optics and Photonics Vol.3 No.3
An Nd:YAG slab structure was designed for a high-power zigzag slab laser amplifier based on computational simulation of the wavefront distortion. For the simulation, the temperature distribution in the slab was calculated at first by thermal analysis. Then, the optical path length (OPL) was obtained by a ray tracing method for the corresponding refractive index variation inside the slab. After that, the OPL distribution of the double-pass amplified beam was calculated by summing the results obtained for the first and second passes. The amount of wavefront distortion was finally obtained as the peak-to-valley value of the OPL distribution. As a result of this study, the length and position of the gain medium were optimized by minimizing the transverse wavefront distortion. Under the optimized conditions, the transverse wavefront distortion of the double-pass amplified beam was less than 0.2 µm for pump power of 14 kW.