원지와 영여자로부터 분리한 8종 화합물의 항균효과

진휘승(Hwi Seung Chin),손락호(Rak Ho Son),이용화(Yong-Hwa Lee),함아름(Ahrom Ham),마응천(Woongchon Mar),김원기(Won-Ki Kim),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.2

The chemical structures of eight compounds purified from two plants (Polygala tenuifolia Willdenow and Dioscorea batatas Decene) were determined and their anti-microbial activity against three microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans) was tested. The three micro organisms were cultured in 96-well plates or Petri dishes without (control) or with the eight compounds added at concentrations of 100 to 0.01 μM (wt/vol). The growth of the microorganisms in the medium was examined after a 24-h incubation. The inhibitory effect of each compound on the growth of the microorganisms was calculated from the optical density measured at 595 nm, turbidity, and size of the inhibition zone around the treated paper disc. The minimum inhiitory concentration (MIC) of compounds 4 to 7 against S. aureus was 0.08, 0.05, 1.3 and 0.02 μM, respectively, and 0.09, 0.1, 0.2 and 100 μM against C. albicans. The IC50 (50% inhibition) values of compounds 5 and 6 were 3.1 and 6.4 μM against S. aureus, respectively, and 10 and 2.4 μM against C. albicans. Therefore, compounds 4 to 6 were the most potent anti-microbial agents among the eight compounds tested.

항비만에 대한 생약복합물의 효과

진휘승(Hwi Seung Chin),박금주(Keum Ju Pack),박승희(Seung Hee Pack),김재기(Jae Ki Kim) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.1

민간요법 중에서 비만 치료에 사용되는 4종의 식물인 지유, 옥발, 적하수오 및 삼백초를 동량으로 혼합하여 10배의 물을 사용하여 가열추출 후 감압농축 하여 HEM을 조제하였고, 이렇게 조제된 HEM을 사용하여 0~100 ㎍/mL 농도로 3T3-L1의 지방축적 억제율을 측정한 결과 농도가 증가함에 따라 유의적으로 증가하였으며, 동일 농도에서 세포독성은 없었다. 또한 지방 축적에 관여하는 효소인 GPDH의 활성을 억제함으로써 세포내 지방이 축적되는 것을 억제하였다. 5주간의 동물실험 결과 대조군과 비교하여 HEM1과 HEM2군 모두 체중 변화와 식이효율이 줄어드는 것으로 나타났으며, 혈액 분석에서 HEM군은 혈중 중성지방과 총콜레스테롤에 효과를 보이지 않았다. 해부학적인 측면에서 부고환 및 신장 주위의 지방무게와 간 무게 또한 낮았다. 이러한 결과를 통해, 본 실험에서 사용한 생약복합물은 항비만 소재로써 체지방 축적억제에 효과성이 있음을 확인하였다. In this study, we investigated the anti-obesity activity of the herbal extract mixture (HEM). The inhibitory effect of HEM on triglyceride accumulation of 3T3-L1 preadipocyte was examined by Oil-Red O staining. HEM inhibited the triglyceride accumulation of 3T3-L1 preadipocyte cell and reduced glycerol-3-phosphate dehydrogenase (GPDH) activity. We further investigated the effect of HEM in prevention of obesity in male ICR mouse for 5 weeks. Experimental groups were divided into high fat diet group (HFD), HFD supplemented with 100 ㎎/㎏ HEM group (HEM1) and HFD supplemented with 200 ㎎/㎏ HEM group (HEM2). Body weight and food efficiency ration of HEM1 and HEM2 was decreased by 52% and 50% and by 45% and 50%, respectively. The amount of adipocyte in body weight was decreased. Blood triglyceride and total cholesterol of HEM1 was significantly decreased. These results indicate that HEM may be useful in preventing obesity.

뽕나무에서 분리한 Steppogenin과 Oxyresveratrol의 효모 α-Glucosidase의 억제효과

진휘승(Hwi Seung Chin),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.5

α-Glucosidase inhibitor is a target in the treatment of type II diabetes through the mainly inhibition of glucose levels after meals. In this study, we purified steppogenin and oxyresveratrol from the stem of Morus alba L. and examined their inhibitory activity against yeast α-glucosidase. Steppogenin and oxyresveratrol were inhibited yeast α-glucosidase in a dose dependent manner. The IC50 activities (50% inhibition) were 34.4 and 9.3 μM, respectively. The kinetic inhibition of steppogenin showed noncompetitive inhibition (Km: 1.1×10-3 M; Ki: 1×10-5 M), meanwhile oxyresveratrol showed competitive inhibition (Km: 4.3×10-3 M; Ki: 3.4×10-6 M) against yeast α-glucosidase. These results indicate that steppogenin and oxyresveratrol are noncompetitive and competitive inhibitors, respectively, against yeast α-glucosidase.

2002 년도 한국임학회 학술연구 발표논문집 / 포스터 발표 : 조록나무 ( Distylium racemosum Sieb. Et Zucc. ) 추출물의 α - amylase 및 α - glucosidase 의 저해 활성 효과

이위영,진휘승,안진권,권영진,이해익 한국임학회 2002 한국임학회 정기학술발표논문집 Vol.2002 No.-

Cadmium Toxicity and Calcium Effect on Growth and Photosynthesis of Tobacco

노광수,진휘승,Roh Kwang Soo,Chin Hiw Seung Korean Society of Life Science 2005 생명과학회지 Vol.15 No.3

담배의 생장과 광합성 색소 및 효소에 대한 카드뮴의 독성과 이에 대한 칼슘의 영향을 연구하였다. 카드뮴에 의한 생장과 엽록소 수준에서의 억제 현상은 칼슘에 의해 회복되었다. 특히, 엽록소의 함량은 카드뮴만 처리한 것 보다 카드뮴과 칼슘을 같이 처리하였을 때 현저하게 증가하였다. 또한 rubisco의 활성과 함량은 카드뮴을 처리한 것 보다 처리하지 않았을 때 현저하게 감소하였으며, 카드뮴의 이러한 효과는 칼슘에 의해 환원되었다. Rubisco activase의 활성과 함량도 rubisco와 같은 경향을 보였다. Rubisco에서 카드뮴과 칼슘에 의해 유도되는 이와 같은 변화는 rubisco activase에 의해 기인됨을 의미한다. This investigation was performed to study Cd toxicity and the influence of Ca on Cd toxicity in growth, and photosynthetic pigments and enzymes in tobacco. Cd inhibited both growth and level of chlorophyll, but the inhibition was compensated by the treatment of Ca. Especially, chlorophyll content was significantly increased by the combination of Cd and Ca treatment compared with Cd treatment alone. In addition, activity and content of rubisco by Cd treatment was also significantly lesser than the non-treated control. The highly reduced activity of rubisco was minimized by the combined treatment of Ca to Cd. Rubisco activase activity and content also showed a pattern of change similar to the rubisco level, suggesting that Cd- and Ca-induced changes of rubisco could be caused by rubisco activase.

머루 줄기와 자소자로부터 분리한 Resveratrol 올리고머와 Flavonoid의 항균효과

손락호(Rak Ho Son),진휘승(Hwi Seung Chin),함아름(Ahrom Ham),마응천(Woongchon Mar),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.1

We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, ε-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration (IC50) of compound 2 against S. aureus was 7.2 μM. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 μM, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.

Streptozotocin 유발 당뇨쥐에서 항당뇨 생약 복합물의 혈당강하 효과

박금주(Keum Ju Park),진휘승(Hwi Seung Jin),박승희(Seung Hee Park),김은호(Eun Ho Kim),김재기(Jae Ki Kim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.12

STZ를 이용한 당뇨유발은 독성물질에 의한 췌장 세포의 파괴를 인위적으로 유도시킴으로써 일정하게 고혈당을 유지 시켜주는 방법이다. 장기적인 고혈당은 인슐린의 민감도를 감소시키고 췌장기능을 저하시키며 잠재적으로는 당뇨병의 악화 및 심혈관 합병증을 유도한다(28,29). 이에 본 연구에서는 STZ로 당뇨를 유발한 흰쥐에 AD복합물을 단회 및 반복 투여한 후 혈당변화에 미치는 영향을 조사한 결과 AD복합물의 단회 투여에서 시간별(0~4시간)로 혈당변화가 관찰되었다. DC군의 공복혈당은 437.3±28 ㎎/㎗에서 3시간 후의 혈당이 443.3±28 ㎎/㎗로 거의 변화가 없었던 반면 AD1군은 414.0±19 ㎎/㎗에서 358±35 ㎎/㎗로 AD2군은 401.0±28 ㎎/㎗에서 351±16 ㎎/㎗로 혈당이 낮춰졌다. 또한 경구당부하 실험에서는 DC군에 비해 AD복합물 투여군이 포도당 투여 1시간 후의 혈당상승을 억제하고 빠른 속도로 원래의 혈당으로 회복시키는 역할을 하는 것으로 확인되었다. 이러한 결과들이 AD복합물의 장기투여에서도 나타나는지 확인하기 위하여 STZ로 당뇨를 유발시킨 흰쥐에 4주 동안 AD복합물을 투여하고 1주마다 혈당변화를 측정한 결과 DC군은 4주 동안 투여 전(0주) 혈당인 385±9 ㎎/㎗를 마지막 주인 4주까지 365±6 ㎎/㎗로 꾸준히 비슷하게 유지한 반면, AD1군은 투여 전 혈당인 349±9 ㎎/㎗에서 260±8 ㎎/㎗로 감소한 것으로 나타났고 AD2군은 투여 전 혈당인 430±10 ㎎/㎗에서 267±38 ㎎/㎗로 공복 시 혈당저하 효능을 갖는 것으로 나타났다. 또한 AD복합물의 투여는 당뇨로 인한 체중감소를 억제하는 효과가 나타났다. 이러한 결과는 체내의 당대사를 촉진시켜 공복혈당감소와 체중감소억제 효과가 나타난 것으로 사료된다. This study was performed to investigate the hypoglycemic effect of single and repeated oral administration of medicinal herbal mixture (AD) in streptozotocin (STZ) induced diabetic rats. Angelica decursiva, Lycium chinense and Adenophora triphylla var. japonica Hara were selected by oral glucose tolerance test (OGTT) and mixed for AD mixture. In an oral glucose tolerance test, the AD inhibited the increase in blood glucose levels at 1 hr and 2 hr and decreased incremental glycemic response area under the curve. In a single administration of AD1 (100 ㎎/㎏) and AD2 (500 ㎎/㎏), significant reductions by 5.3% and 12.3% were observed in fasting blood glucose level for 4 hours. During the 1 month of the experimental period, AD1 and AD2 was given to the STZ induced diabetic rats. At 4th week, the fasting blood glucose levels of AD1 and AD2 caused a fall of 25.5% and 37.9%, respectively. In addition, the body weights were decreased by 7.7% (AD1) and 1.7% (AD2), respectively, compared with diabetic control (DC, decreasing of 10.2%). This study suggests that AD could be potentially useful for fasting and post-prandial hyperglycemia treatment and all these effects concluded to the use of this plant extract to manage diabetes mellitus.