Insulin-like Growth Factor가 치주인대세포의 증식에 미치는 효과에 관한 연구

조종만,김형찬,이종흔 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

Insulin-like Growth Factor(IGF-I) has the potent chemotactic and proliferative effect on preosteoblasts and hematocytes. There were many researches on bone cells, but now there are insufficient data of the effect of IGF-I on the periodontal ligament(PDL) cells. The purpose of this study is to evaluate the effects of IGF-I on the PDL cells. Human PDL cells were prepared from the first premolar tooth extracted for the orthodontic treatment and were cultured in DMEM/10% FBS at a CO_2 incubator(37℃). The concentrations of IGF-I were as follows: 3ng/㎖, 6ng/㎖, 12ng/㎖, 25ng/㎖, 50ng/㎖, 100ng/㎖ and 150ng/㎖. In the IGF-I group, cell numbers were estimated by counting cells under the microscope at 2 days after. Protein determination was made by the method of Lowry et al. The observed results were as follows: 1. The IGF-I group showed the concentration-dependent increase of cell numbers at 2 days after. IGF-I stimulated significantly PDL mitosis at all concentrations. 2. The maximum effect of PDL proliferation was observed at the concentration of 25ng/㎖ IGF-I. Its effect was about twofold than that of the control group. At that concentration, cell numbers were(12.28±1.10)×10^4cell/㎖ 3. Protein contents of PDL cells was increased in all experimental groups and the degree of increase in protein contents was highest at concentration of 25ng/㎖ IGF-I

SCA(sinus crestal approach) kit를 이용한 새로운 상악동 거상술: 증례보고

조종만 대한치과이식임플란트학회 2008 The Korean Academy of Implant Dentistry Vol.27 No.2

전치부에서의 연조직 관리를 통한 심미적 접근

조종만,Cho, Jong-Mahn 대한심미치과학회 1999 Journal of the Korean Academy of Esthetic Dentistr Vol.8 No.1

A tooth solely can not be an exclusive factor to attain our goal, esthetics. Other consideration, such a proportion of individual tooth, harmonized arrangement of the dentition and surrounding tissues including gingiva, lip and face should be taken into account. Esthetic gingival pattern and incisal level in the anterior area are the very important factors for esthetic restoration of the smile. We often propose adequate periodontal and prosthodontic approaches in order to improve the esthetics of the anterior area. Here, I would like to share some cases which include soft tissue management for ovate pontic placement, osteoplasty, resin filling for severe cervical caries, and bleaching approach to discolored root caused by endodontic treatment, and so on.

염색체 자동분류 시스템의 설계 및 구현 ( Design and Implementation of Computer Aided Automated Karyotyping System )

조종만,문철홍,정상봉,홍승홍 대한전자공학회 1988 대한전자공학회 학술대회 Vol.1988 No.11

ROS 17/2.8 세포배양에서 골기질 형성 및 석회화와 관련되는 SPARC, bone sialoprotein, osteopontin 및 alkaline phosphatase의 특성에 관한 연구

조종만,김형찬 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

To determine the role of noncollagenous proteins and alkaline phosphatase during matrix formation and mineralization, the biosynthesis of secreted protein acidic and rich in cysteine (SPARC), bone sialoprotein (BSP) and osteopontin (OP), Northern blot analysis of SPARC and alkaline phosphatase (ALP) mRNAs and the specific activity of ALP were studied in cultures of clonal osteoblastic rat osteosarcoma ROS 17/2.8 cells. First, at the culture conditions of confluence, matrix formation and minerlization, ['S-methionine-labelled proteins were specifically immunoprecipitated with antibodies to SPARC, BSP and OP and analysed by SDS/PAGE on 15% cross-linked mini-gels under reducing conditions. The expression of SPARC and ALP mRNAs was estimated by Northern blot analysis and the ALP specific activity (pmol/min/106 cells) was measured by the methods of Lowry et al.'' and Majeska andRodan.''' Second, to determine the inductive effects of dexamethasone (Dex) on the expression of SPARC and ALP mRNAs and the ALP specific activity in mineralizing culture, the confluent cultures of ROS 17/2.8 cells were incubated for 0, 4, 7, and 14 days in contral group [ascorbic acid (AA), J3-glycerophosphate (/3-GP)] and Dex group (AA, /3-GP and Dex). Analysis of mRNA by Northern hybridization using the SPARC and ALP cDNA probes was performed and the ALP specific activity was measured and analysed by spectra-photometry. The results were as follows. 1. ROS 17/2.8 cells incubated in the culture condition for matrix formation exhibited multilayers and in the culture condition for mineralization they indicated many foci-like cell aggregates (bone nodules). 2. SPARC was abundant in culture medium and cell extract, but not detected in E extract of the culture condition for mineralization. BSP was present only in cell extract and OP in medium and cell extract. 3. The strongest band of SPARC was revealed in medium of the culture condition for mineralization. In BSP, it was present in cell extract of the culture condition for confluence, and in OP, it was in medium of the culture condition for matrix formation. 4. Northern blot analysis revealed that levels of SPARC mRNA the in culture conditions for matrix forma_ Lion and mineralization comparing with those in culture condition for confluence were shown to be increased by 2.1-and 31-fold, respectively. However, levels of ALP mRNA were increased markedly only in the culture condition for mineralization. 5. In mineralizing culture, Northern hybridization analysis showed a strong induction of SPARC and ALP mRNAs in Dex-treated condition. Especially, maximum effects of Dex were indicated at 7days in both SPARC and ALP mRNAs. 6. ALP specific activites in the culture condition for matrix formation and mineralization comparing with those in the culture condition for confluenece were increased by 1.8-and 3.5-fold, respectively. In Dextreated culture, ALP specific activities at 4, 7 and 14 days were increased by 4.5-, 3.5- and 2.6-fold, respectively, but cell proliferation was shown to be inhibited.

염색체 자동분류 시스템의 구현

조종만,홍승홍 대한의용생체공학회 1988 의공학회지 Vol.9 No.2

This paper describes the design and implementation of personal computer assisted karyotyping system of Giemsa stained chromosomes. The system consists of an Image Acquisition Module being capable of $256 {\times} 256$ pixels and its relevent software modules optimized for karyotyping. The results of karyotyping using this system with an image of chromosomes taken from the Rana Amurensis are acceptable. As a result of this study we can save our load oweing to the conventional hand- karyotyping and the high-cost computer.

CAD/CAM Zirconia All Ceramic Restoration and Red-White Esthetics

조종만,Jo, Jong-Man 대한심미치과학회 2004 Journal of the Korean Academy of Esthetic Dentistr Vol.13 No.1

For the prosthodontic results which are compatible with esthetics to be fulfilled, the first prerequisite would be periodontal intervention and stability, the second one may be functional competency and the ultimate goal should be ascribed to esthetic considerations. Other words, esthetic dentistry is a whole entity which encompasses the biological stability of the periodontium, physical accuracy of the prosthodontic structure and finally, the beauty which can be found in natural dentition. It also implies the harmonized lip line which reveals the well-balanced tooth morphology and health gum profiles (Red-White Esthetics). Largely, there lie some differences in the input system of the 3-dimentional data from the prepared abutments between respective computer-assisted systems available now. But the manufacturing systems (CAM) are very similar between them, to say, comprise numeric control systems with whole 3-dimensional milling units according to the restorations to be made. Now the author is going to present CAD/CAM Zirconia All Ceramic Restoration on the topics for the Red-White Esthetics, periodontal control and maintenance, treatment for the discolored teeth, post & core build-up works for the devitalized teeth, characteristics of the Zirconium oxide All Ceramics, fabrication procedures, clinical considerations and its application to diverse clinical situations.

재활 훈련중인 환자를 위한 다채널 무구속 심박동수 모니터링 시스템

조종만,최정현,박준호,남태우,은종민,Cho, J.M.,Choi, J.H.,Park, J.H.,Nam, T.W.,Eun, J.M. 대한의용생체공학회 2008 의공학회지 Vol.29 No.3

This research focused on the development of wireless telemetry system that can monitor heart rates of multiple rehabilitation patients in real time without constraint. The whole system consists of the multiple patient's side devices (PSDs) and one central monitoring system (CMS). The PSD consists of a microphone, amplifier, filter, microcontroller, and RF (Radio Frequency) modem. In addition, the PSD was designed to be wearable and low power consumption. The CMS consists of an RF modem and general PC and it was designed to monitor heart rates from multiple patients simultaneously. The system warns an alarm signal when a patient's heart rate exceeds the pre-set range for each patient. This system can be useful to monitor the heart rate of exercising rehabilitation patients and control the patients condition and the exercising level.

ECG신호의 실시간 전송 및 처리 시스템의 구현 ( Implementation of Real Time Transmission and Processing System for ECG Signal )

조종만,장원영,문철홍,장원석,홍승홍 대한전자공학회 1988 대한전자공학회 학술대회 Vol.1988 No.7

재난관리기관간 협력체계구축을 위한 영역별 상대적 중요도 분석

조종묵(Jong Mook Cho)(趙鍾黙,),류상일(Sang Il Ryu)(柳賞溢),이재은(Jae Eun Lee)(李在恩) 위기관리 이론과 실천 2011 Crisisonomy Vol.7 No.1

우리나라의 경우 1990년대 중반이후 재난관리에 국가적 차원에서 법과 제도를 제ㆍ개정하면서 지속적인 노력을 기울임에도 불구하고, 유사한 사건사고가 발생하면 재난관리에 실패를 하고 또 다시 문제점과 한계를 드러내고 있다. 이에 본 연구는 재난관리의 효과성 향상을 위해 다양한 재난관리 참여기관들이 협력체계를 구축하기 위해서는 무엇이 우선시 되어야 하는가를 전문가를 대상으로 하여 실증연구를 실시하고자 하였다. 결과적으로, 재난관리 전문가(중앙ㆍ지방정부, 학계/연구소, 군, 경찰, 소방)를 대상으로 실시한 참여기관의 협력요인간 상대적 중요도 및 우선순위를 분석한 결과, 조직화→의사소통 및 의사결정→조정 메커니즘→계획→협력 조직문화의 순으로 중요도가 나타났으며, 또한 재난관리 참여기관 협력체계 구축을 위한 요인간 중요도 및 우선순위에 대한 복합가중치를 보면, 지식 및 정보 공유→ 협력 기구 마련→통합 지휘체계 마련→정기회의 및 결정→협력 운영절차 마련순으로 중요도가 측정되었다. 이는 재난관리 참여기관간 협력체계를 구축하기 위하여 협력 기구 및 운영 절차 등 관련규정과 제도 등을 정비하고 참여기관 간 재난정보 등을 공유하는 활동이 중요하다. 또한 초기 재난발생시 효과적인 대응을 위하여 통합지휘체계를 마련함으로서 일사불란한 재난관리 활동이 요구된다. This study examines the relative importance and priority of the policy area and policy variables in the field of the Cooperative factors of Agencies in Disaster Management by using analytic hierarchy process(AHP). According to the results of surveying disaster management specialists (central-local governments, universities/research institutes, army, police, fire service) on the relative importance and priority of factors related to cooperation among participating institutions, importance was high in order of organization → communication and decision making→ coordination mechanism → planning → cooperative organizational culture. In addition, the combined weight of the importance and priority of factors for building a cooperation system among institutions participating in disaster management was high in order of knowledge and information sharing→ establishment of a cooperation organization → establishment of an integrated command system → regular meetings and decision making → establishment of cooperative operation procedures. That is, in order to build a cooperation system among institutions participating in disaster management, it is important to improve relevant laws and systems including the cooperation organization and operation procedures and to share information on disasters among the participating institutions. Furthermore, it is required to execute well-organized disaster management activities by establishing an integrated command system for early and effective coping with disasters.